Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004354A_C01 KMC004354A_c01
(479 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB08634.1| pectinesterase like protein [Arabidopsis thaliana] 150 7e-36
ref|NP_569041.1| pectinesterase (pectin methylesterase) family; ... 150 7e-36
gb|AAM61328.1| pectinesterase-like protein [Arabidopsis thaliana] 149 2e-35
dbj|BAC10807.1| contains ESTs D23897(R0531),AU181257(C11117),AU1... 146 1e-34
gb|AAD41439.1|AC007727_28 Strong similarity to gb|X96932 ascorba... 130 6e-30
>dbj|BAB08634.1| pectinesterase like protein [Arabidopsis thaliana]
Length = 544
Score = 150 bits (379), Expect = 7e-36
Identities = 68/88 (77%), Positives = 74/88 (83%)
Frame = -3
Query: 477 QWTDASRSTYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQVYLR 298
QWT A RS +NLVDALTRHT QVYP SWT ILVSLDNQGMWN+RSAIWERQY GQQ YL+
Sbjct: 456 QWTPAKRSLHNLVDALTRHTTQVYPESWTTILVSLDNQGMWNMRSAIWERQYSGQQFYLK 515
Query: 297 VWNAQQSLANEYGIPNNALLCGKAIGHH 214
VWN+ QSLANEY P+N LCGKA+G H
Sbjct: 516 VWNSVQSLANEYNPPDNLQLCGKAVGRH 543
>ref|NP_569041.1| pectinesterase (pectin methylesterase) family; protein id:
At5g66920.1, supported by cDNA: 116979., supported by
cDNA: gi_20453069 [Arabidopsis thaliana]
gi|20453070|gb|AAM19780.1| AT5g66920/MUD21_18
[Arabidopsis thaliana] gi|23507791|gb|AAN38699.1|
At5g66920/MUD21_18 [Arabidopsis thaliana]
Length = 546
Score = 150 bits (379), Expect = 7e-36
Identities = 68/88 (77%), Positives = 74/88 (83%)
Frame = -3
Query: 477 QWTDASRSTYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQVYLR 298
QWT A RS +NLVDALTRHT QVYP SWT ILVSLDNQGMWN+RSAIWERQY GQQ YL+
Sbjct: 458 QWTPAKRSLHNLVDALTRHTTQVYPESWTTILVSLDNQGMWNMRSAIWERQYSGQQFYLK 517
Query: 297 VWNAQQSLANEYGIPNNALLCGKAIGHH 214
VWN+ QSLANEY P+N LCGKA+G H
Sbjct: 518 VWNSVQSLANEYNPPDNLQLCGKAVGRH 545
>gb|AAM61328.1| pectinesterase-like protein [Arabidopsis thaliana]
Length = 546
Score = 149 bits (375), Expect = 2e-35
Identities = 67/88 (76%), Positives = 73/88 (82%)
Frame = -3
Query: 477 QWTDASRSTYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQVYLR 298
QWT RS +NLVDALTRHT QVYP SWT ILVSLDNQGMWN+RSAIWERQY GQQ YL+
Sbjct: 458 QWTPTKRSLHNLVDALTRHTTQVYPESWTTILVSLDNQGMWNMRSAIWERQYSGQQFYLK 517
Query: 297 VWNAQQSLANEYGIPNNALLCGKAIGHH 214
VWN+ QSLANEY P+N LCGKA+G H
Sbjct: 518 VWNSVQSLANEYNPPDNLQLCGKAVGRH 545
>dbj|BAC10807.1| contains ESTs D23897(R0531),AU181257(C11117),AU101619(R0531),
AU181258(C11117)~pectinesterase-like protein [Oryza
sativa (japonica cultivar-group)]
Length = 544
Score = 146 bits (368), Expect = 1e-34
Identities = 64/86 (74%), Positives = 73/86 (84%)
Frame = -3
Query: 477 QWTDASRSTYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQVYLR 298
QWT+ R+TYNLVDA RHT QVYPN W+AILVSLDNQGMWNLRSA W+RQYLGQQ+Y+R
Sbjct: 456 QWTENQRTTYNLVDAQARHTVQVYPNGWSAILVSLDNQGMWNLRSANWDRQYLGQQLYMR 515
Query: 297 VWNAQQSLANEYGIPNNALLCGKAIG 220
VW QQS +NEY IP NA+LCG+A G
Sbjct: 516 VWTPQQSFSNEYSIPTNAILCGRAAG 541
>gb|AAD41439.1|AC007727_28 Strong similarity to gb|X96932 ascorbate oxidase-related protein
PS60 from Nicotiana tabacum and is a member of the
PF|00394 Multicopper oxidase family. This gene is cut
off. [Arabidopsis thaliana]
Length = 359
Score = 130 bits (328), Expect = 6e-30
Identities = 60/88 (68%), Positives = 69/88 (78%)
Frame = -3
Query: 477 QWTDASRSTYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQVYLR 298
+W+ ASR YNL DA++R T QVYP SWTAI VSLDN GMWNLRS +WERQYLGQQ Y+R
Sbjct: 258 KWSAASRKVYNLNDAVSRCTVQVYPRSWTAIYVSLDNVGMWNLRSELWERQYLGQQFYMR 317
Query: 297 VWNAQQSLANEYGIPNNALLCGKAIGHH 214
V+ SL +EY IP NALLCG+A GHH
Sbjct: 318 VYTPSTSLRDEYLIPKNALLCGRATGHH 345
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 397,048,297
Number of Sequences: 1393205
Number of extensions: 8241980
Number of successful extensions: 19736
Number of sequences better than 10.0: 131
Number of HSP's better than 10.0 without gapping: 19365
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19734
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13397825772
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
