Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004335A_C01 KMC004335A_c01
(599 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_564219.1| G-protein beta family; protein id: At1g24530.1,... 169 3e-41
ref|NP_173823.1| G-protein beta family; protein id: At1g24130.1 ... 134 1e-30
ref|NP_199823.1| G-protein beta family; protein id: At5g50120.1 ... 111 6e-24
emb|CAD41096.1| OSJNBb0011N17.13 [Oryza sativa (japonica cultiva... 108 4e-23
ref|NP_175369.1| En/Spm-like transposon protein, putative; prote... 97 2e-19
>ref|NP_564219.1| G-protein beta family; protein id: At1g24530.1, supported by cDNA:
gi_19310648 [Arabidopsis thaliana]
gi|9743339|gb|AAF97963.1|AC000103_13 F21J9.19
[Arabidopsis thaliana] gi|15028341|gb|AAK76647.1|
unknown protein [Arabidopsis thaliana]
gi|19310649|gb|AAL85055.1| unknown protein [Arabidopsis
thaliana]
Length = 418
Score = 169 bits (427), Expect = 3e-41
Identities = 84/118 (71%), Positives = 97/118 (82%)
Frame = -2
Query: 598 GSVLFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCLINVSDFLLSGSADRTVRVW 419
GSVLFSG+CDRSILVWERED++N+M V GALRGH+KAIL L NVSD LLSGSADRTVR+W
Sbjct: 292 GSVLFSGSCDRSILVWEREDTSNYMAVRGALRGHDKAILSLFNVSDLLLSGSADRTVRIW 351
Query: 418 KRGFDGPFCCLAVLDGHRKPVKSLAAVAESDQNSPDGVVSIFSGSLDGEIRVWQVSLT 245
+RG D + CL VL GH KPVKSLAAV E + D VVSI SGSLDGE++ W+VS+T
Sbjct: 352 RRGPDSSYSCLEVLSGHTKPVKSLAAVREKEL---DDVVSIISGSLDGEVKCWKVSVT 406
Score = 48.1 bits (113), Expect = 8e-05
Identities = 34/118 (28%), Positives = 62/118 (51%), Gaps = 2/118 (1%)
Frame = -2
Query: 589 LFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCL-INVSDFLLSGSADRTVRVW-K 416
++S + D+++ +W D + +++ H+ A+ + ++ + + +GSADR +RVW K
Sbjct: 208 IYSVSWDKTLKIWRASD----LRCKESIKAHDDAVNAIAVSTNGTVYTGSADRRIRVWAK 263
Query: 415 RGFDGPFCCLAVLDGHRKPVKSLAAVAESDQNSPDGVVSIFSGSLDGEIRVWQVSLTS 242
+ +A L+ H+ V +LA + DG V +FSGS D I VW+ TS
Sbjct: 264 PTGEKRHTLVATLEKHKSAVNALAL-------NDDGSV-LFSGSCDRSILVWEREDTS 313
Score = 33.5 bits (75), Expect = 2.1
Identities = 22/80 (27%), Positives = 37/80 (45%)
Frame = -2
Query: 499 HEKAILCLINVSDFLLSGSADRTVRVWKRGFDGPFCCLAVLDGHRKPVKSLAAVAESDQN 320
H A+ L F+ S S D+T+++W+ C + H V ++A
Sbjct: 194 HADAVTALAVSDGFIYSVSWDKTLKIWRA---SDLRCKESIKAHDDAVNAIAV------- 243
Query: 319 SPDGVVSIFSGSLDGEIRVW 260
S +G ++++GS D IRVW
Sbjct: 244 STNG--TVYTGSADRRIRVW 261
>ref|NP_173823.1| G-protein beta family; protein id: At1g24130.1 [Arabidopsis
thaliana] gi|7486376|pir||T00642 hypothetical protein
F3I6.5 - Arabidopsis thaliana gi|2829890|gb|AAC00598.1|
Hypothetical protein [Arabidopsis thaliana]
Length = 415
Score = 134 bits (336), Expect = 1e-30
Identities = 72/127 (56%), Positives = 93/127 (72%), Gaps = 7/127 (5%)
Frame = -2
Query: 598 GSVLFSGACDRSILVWER----EDSANHMVVSGALRGHEKAILCLINVSDFLLSGSADRT 431
G VL+SGACDRSILVWER +D HM V GALRGH KAI+CL SD +LSGSAD++
Sbjct: 290 GKVLYSGACDRSILVWERLINGDDEELHMSVVGALRGHRKAIMCLAVASDLVLSGSADKS 349
Query: 430 VRVWKRGF--DGPFCCLAVLDGHRKPVKSLA-AVAESDQNSPDGVVSIFSGSLDGEIRVW 260
+RVW+RG + CLAVL+GH KPVKSLA +V++SD NS D ++SGSLD ++VW
Sbjct: 350 LRVWRRGLMEKEGYSCLAVLEGHTKPVKSLAVSVSDSDSNS-DYSCMVYSGSLDLSLKVW 408
Query: 259 QVSLTSL 239
+ ++S+
Sbjct: 409 NLRVSSI 415
Score = 53.5 bits (127), Expect = 2e-06
Identities = 37/115 (32%), Positives = 58/115 (50%), Gaps = 1/115 (0%)
Frame = -2
Query: 598 GSVLFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCLINVSD-FLLSGSADRTVRV 422
GS+L+S + DRS +W D + + H+ AI ++ D F+ +GSAD+ ++V
Sbjct: 204 GSLLYSASWDRSFKIWRTSD---FKCLDSIEKAHDDAINAIVVSKDGFVYTGSADKKIKV 260
Query: 421 WKRGFDGPFCCLAVLDGHRKPVKSLAAVAESDQNSPDGVVSIFSGSLDGEIRVWQ 257
W + D +A L H V +LA S DG V ++SG+ D I VW+
Sbjct: 261 WNKK-DKKHSLVATLTKHLSAVNALAI-------SEDGKV-LYSGACDRSILVWE 306
>ref|NP_199823.1| G-protein beta family; protein id: At5g50120.1 [Arabidopsis
thaliana] gi|10177223|dbj|BAB10298.1| contains
similarity to GTP-binding regulatory protein and
WD-repeat protein~gene_id:MPF21.14 [Arabidopsis
thaliana]
Length = 388
Score = 111 bits (278), Expect = 6e-24
Identities = 57/115 (49%), Positives = 76/115 (65%)
Frame = -2
Query: 598 GSVLFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCLINVSDFLLSGSADRTVRVW 419
GS+L SG D SILVWER+D + +VV G LRGH +++LCL VSD L SGSAD+TVR+W
Sbjct: 272 GSLLHSGGSDGSILVWERDDGGD-IVVVGMLRGHTESVLCLAVVSDILCSGSADKTVRLW 330
Query: 418 KRGFDGPFCCLAVLDGHRKPVKSLAAVAESDQNSPDGVVSIFSGSLDGEIRVWQV 254
K + CLA+L+GH PVK L + + + I+SG LD +++VWQV
Sbjct: 331 KCSAK-DYSCLAMLEGHLGPVKCLTGAFRDSRKADEASYHIYSGGLDSQVKVWQV 384
Score = 39.7 bits (91), Expect = 0.030
Identities = 27/122 (22%), Positives = 56/122 (45%), Gaps = 8/122 (6%)
Frame = -2
Query: 598 GSVLFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCL-INVSDFLLSGSADRTVRV 422
G++L+S + DR++ +W D + H+ AI + ++ + + +GS+D+ ++V
Sbjct: 177 GTLLYSVSWDRTLKIWRTTD---FKCLESFTNAHDDAINAVALSENGDIYTGSSDQRIKV 233
Query: 421 WKRGFD-------GPFCCLAVLDGHRKPVKSLAAVAESDQNSPDGVVSIFSGSLDGEIRV 263
W++ + +A+L H + +LA + + SG DG I V
Sbjct: 234 WRKNINEENVKKKRKHSLVAILSEHNSGINALALSGTNGS-------LLHSGGSDGSILV 286
Query: 262 WQ 257
W+
Sbjct: 287 WE 288
Score = 33.5 bits (75), Expect = 2.1
Identities = 21/68 (30%), Positives = 35/68 (50%), Gaps = 7/68 (10%)
Frame = -2
Query: 598 GSVLFSGACDRSILVWER-------EDSANHMVVSGALRGHEKAILCLINVSDFLLSGSA 440
G L++G+ D + +W E S+N V++G RG A+ L+ ++D L +
Sbjct: 48 GKRLYTGSNDGVVRLWNANTLETLAEASSNGDVITGE-RGGGGAVKSLVILADKLFTAHQ 106
Query: 439 DRTVRVWK 416
D +RVWK
Sbjct: 107 DHKIRVWK 114
>emb|CAD41096.1| OSJNBb0011N17.13 [Oryza sativa (japonica cultivar-group)]
Length = 470
Score = 108 bits (271), Expect = 4e-23
Identities = 57/133 (42%), Positives = 83/133 (61%), Gaps = 14/133 (10%)
Frame = -2
Query: 598 GSVLFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCLINVSD------FLLSGSAD 437
G VL+SG DR ++VWEREDSA+HMV GALRGH +A+L + + ++SG+AD
Sbjct: 336 GQVLYSGGNDRCVVVWEREDSASHMVAVGALRGHRRAVLSVACAAGDAADGALVVSGAAD 395
Query: 436 RTVRVWKRGFDG-PFCCLAVLDGHRKPVKSLAAV-------AESDQNSPDGVVSIFSGSL 281
+TVR W+RG DG + C+AV+DGH V+S+AA +D + D + S S
Sbjct: 396 QTVRAWRRGADGRGYYCVAVIDGHGSAVRSVAAALVTAQKKRRADDDGGDEEWRVCSASF 455
Query: 280 DGEIRVWQVSLTS 242
DGE+R+W + + +
Sbjct: 456 DGEVRLWSLRVAA 468
>ref|NP_175369.1| En/Spm-like transposon protein, putative; protein id: At1g49450.1,
supported by cDNA: gi_17979258 [Arabidopsis thaliana]
gi|25405310|pir||B96531 hypothetical protein F13F21.11
[imported] - Arabidopsis thaliana
gi|5430755|gb|AAD43155.1|AC007504_10 Hypothetical
Protein [Arabidopsis thaliana]
Length = 471
Score = 97.1 bits (240), Expect = 2e-19
Identities = 49/124 (39%), Positives = 71/124 (56%), Gaps = 9/124 (7%)
Frame = -2
Query: 595 SVLFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCLINVSDFLLSGSADRTVRVWK 416
+V++ G+ D ++ WER+ H G + GH A+LCL LLSG AD+ + VWK
Sbjct: 347 AVVYCGSSDGTVNFWERQKYLTH---KGTIHGHRMAVLCLATAGSLLLSGGADKNICVWK 403
Query: 415 RGFDGPFCCLAVLDGHRKPVKSLAAVAESDQNSPDGVVS---------IFSGSLDGEIRV 263
R DG CL+VL H PVK LAAV E++++ DG ++SGSLD ++V
Sbjct: 404 RNGDGSHTCLSVLMDHEGPVKCLAAVEEAEEDHNDGDDGGEKGDQRWIVYSGSLDNSVKV 463
Query: 262 WQVS 251
W+V+
Sbjct: 464 WRVT 467
Score = 50.1 bits (118), Expect = 2e-05
Identities = 33/113 (29%), Positives = 58/113 (51%), Gaps = 1/113 (0%)
Frame = -2
Query: 592 VLFSGACDRSILVWEREDSANHMVVSGALRGHEKAILCLIN-VSDFLLSGSADRTVRVWK 416
+L+SG+ D+++ VW DS ++ H+ A+ +++ D + +GSAD T++VWK
Sbjct: 259 LLYSGSWDKTLKVWRLSDSK----CLESIEAHDDAVNTVVSGFDDLVFTGSADGTLKVWK 314
Query: 415 RGFDGPFCCLAVLDGHRKPVKSLAAVAESDQNSPDGVVSIFSGSLDGEIRVWQ 257
R G ++ K ++ A+A N D VV + GS DG + W+
Sbjct: 315 REVQGKEMKHVLVQVLMKQENAVTALA---VNLTDAVV--YCGSSDGTVNFWE 362
Score = 34.7 bits (78), Expect = 0.96
Identities = 26/98 (26%), Positives = 43/98 (43%)
Frame = -2
Query: 544 EDSANHMVVSGALRGHEKAILCLINVSDFLLSGSADRTVRVWKRGFDGPFCCLAVLDGHR 365
ED ++ ++ +R E + L D L +GS + +RVWK L G +
Sbjct: 119 EDDPDNGLIGTVVR-QEGHVYSLAASGDLLFTGSDSKNIRVWKD--------LKDFSGFK 169
Query: 364 KPVKSLAAVAESDQNSPDGVVSIFSGSLDGEIRVWQVS 251
+ A+ + N +F+G DG+IRVW+ S
Sbjct: 170 STSGFVKAIVVTRDN------RVFTGHQDGKIRVWRGS 201
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 553,867,184
Number of Sequences: 1393205
Number of extensions: 13114672
Number of successful extensions: 72956
Number of sequences better than 10.0: 1251
Number of HSP's better than 10.0 without gapping: 57386
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69777
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23426109484
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
