>KMC004305A_C01 KMC004305A_c01
cgaatgcaccccaacagaatgctgaggagtctaaatcatatctatttacagaagcaacaa
taaacaGAAATCAAAACCACCCTGGATGGTGGCAACGATGTACTCAGCGCAAAATGAGCC
GACAATGGTTTCTACATCTCAAATCATAAGTATAAAAAAGACATCTCACAATTAGATAGG
GTCTAAAACCTCTCTTCCCCTCACCAAATATATATCCCTACAAAACTGTGGGCTGTTTGT
TCTAACTCTCTGAAACAGCCAAAAAATCAGATCAATATTCTAATGAAAGGAACATTTGTG
AATCACAACAATTACATTAACGGTTAATTTCCCTCCATCAAGCTCTTTAATCTATTGCGG
GCCTCTTCAGACAGACTGCAAGCTTTGAATCTTCGCCTTTGGGGTGGACATGATTCAACA
GTGCTTTCAATGGAGGACAAACATATAACAATCACAGTGAGATTATCCGATGTGTTTAGG
CGCAATGCTTCCTTGACTAGTTCTCTGGCACATTGCTGGGGATCATCATGCCTTCTTAAT
CCGCGTCGAACAAGACTAACTGGCGAGACTTGGCTATACTTTACATCCCAAATCCCATCA
CAACCAATGATCAAGAACTCATCATCTTCTGTCAAAGTAATCAGTTGAACATCAGGCTCG
GCAATAAGAGGGGATGCAGCGCCAATTGGCAATTTAAAGTCCCAATCCCCCAAGGCACGA
GTTACAGAAAGATAACCATTGAGATATCCATCATCAATAAAGCCACCCAACTCCTCCACT
CTCCTACGCTCAGGTAAATAGCTCGGCCTGTGATCATGGGACATGTCAACAGCTTTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004305A_C01 KMC004305A_c01
         (837 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T52337 phosphoprotein phosphatase (EC 3.1.3.16) 2C [importe...   229  5e-59
ref|NP_175238.1| protein phosphatase-2C, putative; protein id: A...   204  2e-51
dbj|BAB12036.1| putative protein phosphatase [Oryza sativa (japo...   201  8e-51
ref|NP_191785.1| protein phosphatase 2C (PP2C); protein id: At3g...   196  4e-49
dbj|BAB02728.1| protein phosphatase 2C-like protein [Arabidopsis...   192  4e-48

>pir||T52337 phosphoprotein phosphatase (EC 3.1.3.16) 2C [imported] - common ice
           plant gi|3643085|gb|AAC36698.1| protein phosphatase-2C;
           PP2C [Mesembryanthemum crystallinum]
          Length = 359

 Score =  229 bits (583), Expect = 5e-59
 Identities = 114/170 (67%), Positives = 140/170 (82%)
 Frame = -2

Query: 836 KAVDMSHDHRPSYLPERRRVEELGGFIDDGYLNGYLSVTRALGDWDFKLPIGAASPLIAE 657
           +A+DMS DHRP+Y  E+RRVEELGG++DDGYLNG LSV+RALGDWD KLP G+ASPLI+E
Sbjct: 189 EAIDMSQDHRPTYPSEKRRVEELGGYVDDGYLNGVLSVSRALGDWDMKLPKGSASPLISE 248

Query: 656 PDVQLITLTEDDEFLIIGCDGIWDVKYSQVSPVSLVRRGLRRHDDPQQCARELVKEALRL 477
           P+++ I LTEDDEFLIIGCDGIWDV  SQ   VS+VR GL+RHDDP+Q A++LV EALR 
Sbjct: 249 PELRQIILTEDDEFLIIGCDGIWDVISSQ-QAVSIVRWGLKRHDDPEQSAKDLVNEALRR 307

Query: 476 NTSDNLTVIVICLSSIESTVESCPPQRRRFKACSLSEEARNRLKSLMEGN 327
           +T DNLTVI++C SSI+   E   P+ RRF+ CSLS EA + L+SL+EGN
Sbjct: 308 HTIDNLTVIIVCFSSIDHQREQTGPRPRRFR-CSLSAEALSSLRSLLEGN 356

>ref|NP_175238.1| protein phosphatase-2C, putative; protein id: At1g48040.1
           [Arabidopsis thaliana]
           gi|8778520|gb|AAF79528.1|AC023673_16 F21D18.27
           [Arabidopsis thaliana]
           gi|12323084|gb|AAG51521.1|AC051631_1 protein
           phosphatase-2C, putative; 42154-43770 [Arabidopsis
           thaliana]
          Length = 377

 Score =  204 bits (518), Expect = 2e-51
 Identities = 104/169 (61%), Positives = 134/169 (78%), Gaps = 1/169 (0%)
 Frame = -2

Query: 833 AVDMSHDHRPSYLPERRRVEELGGFIDDGYLNGYLSVTRALGDWDFKLPI-GAASPLIAE 657
           AVDMS DHR +Y PERRR+E+LGG+ +DGYLNG L+VTRA+GDW+ K P   ++SPLI++
Sbjct: 212 AVDMSFDHRSTYEPERRRIEDLGGYFEDGYLNGVLAVTRAIGDWELKNPFTDSSSPLISD 271

Query: 656 PDVQLITLTEDDEFLIIGCDGIWDVKYSQVSPVSLVRRGLRRHDDPQQCARELVKEALRL 477
           P++  I LTEDDEFLI+ CDGIWDV  SQ + VS VR+GLRRH DP+QCA EL KEA RL
Sbjct: 272 PEIGQIILTEDDEFLILACDGIWDVLSSQ-NAVSNVRQGLRRHGDPRQCAMELGKEAARL 330

Query: 476 NTSDNLTVIVICLSSIESTVESCPPQRRRFKACSLSEEARNRLKSLMEG 330
            +SDN+TVIVIC SS+ S+ +   PQRRR + C +S+EAR RL++++ G
Sbjct: 331 QSSDNMTVIVICFSSVPSSPKQ--PQRRRLRFC-VSDEARARLQAMLAG 376

>dbj|BAB12036.1| putative protein phosphatase [Oryza sativa (japonica
           cultivar-group)]
          Length = 380

 Score =  201 bits (512), Expect = 8e-51
 Identities = 109/174 (62%), Positives = 125/174 (71%), Gaps = 5/174 (2%)
 Frame = -2

Query: 833 AVDMSHDHRPSYLPERRRVEELGGFIDDGYLNGYLSVTRALGDWDFKLPIGAASPLIAEP 654
           AV+MS DHRP+Y  E  R+ E GG+I+DGYLNG LSVTRALGDWD K+P G+ SPLIAEP
Sbjct: 209 AVEMSRDHRPTYDAEHERITECGGYIEDGYLNGVLSVTRALGDWDMKMPQGSRSPLIAEP 268

Query: 653 DVQLITLTEDDEFLIIGCDGIWDVKYSQVSPVSLVRRGLRRHDDPQQCARELVKEALRLN 474
           + Q  TLTEDDEFLIIGCDGIWDV  SQ   V++VR+GLRRHDDP++CAREL  EA RL 
Sbjct: 269 EFQQTTLTEDDEFLIIGCDGIWDVMSSQ-HAVTIVRKGLRRHDDPERCARELAMEAKRLQ 327

Query: 473 TSDNLTVIVICLSSI----ESTVESCPPQRRRFKAC-SLSEEARNRLKSLMEGN 327
           T DNLTVIVIC  S       + E  P   RR + C SLS EA   LK  +E N
Sbjct: 328 TFDNLTVIVICFGSELGGGSPSSEQAP--IRRVRCCKSLSSEALCNLKKWLEPN 379

>ref|NP_191785.1| protein phosphatase 2C (PP2C); protein id: At3g62260.1, supported
           by cDNA: 20050. [Arabidopsis thaliana]
           gi|11358069|pir||T48018 hypothetical protein T17J13.220
           - Arabidopsis thaliana gi|6899936|emb|CAB71886.1|
           putative protein [Arabidopsis thaliana]
           gi|21554078|gb|AAM63159.1| protein phosphatase-2C
           [Arabidopsis thaliana]
          Length = 383

 Score =  196 bits (498), Expect = 4e-49
 Identities = 102/170 (60%), Positives = 129/170 (75%), Gaps = 1/170 (0%)
 Frame = -2

Query: 836 KAVDMSHDHRPSYLPERRRVEELGGFI-DDGYLNGYLSVTRALGDWDFKLPIGAASPLIA 660
           +A+DMS DH+P  L ERRRVEE GGFI +DGYLN  L+VTRALGDWD KLP G+ SPLI+
Sbjct: 216 RAIDMSEDHKPINLLERRRVEESGGFITNDGYLNEVLAVTRALGDWDLKLPHGSQSPLIS 275

Query: 659 EPDVQLITLTEDDEFLIIGCDGIWDVKYSQVSPVSLVRRGLRRHDDPQQCARELVKEALR 480
           EP+++ ITLTEDDEFL+IGCDGIWDV  SQ   VS+VRRGL RH+DP +CARELV EAL 
Sbjct: 276 EPEIKQITLTEDDEFLVIGCDGIWDVLTSQ-EAVSIVRRGLNRHNDPTRCARELVMEALG 334

Query: 479 LNTSDNLTVIVICLSSIESTVESCPPQRRRFKACSLSEEARNRLKSLMEG 330
            N+ DNLT +V+C  +++   +   P  +R +  SLS EA   L++L++G
Sbjct: 335 RNSFDNLTAVVVCFMTMDRGDKPVVPLEKR-RCFSLSPEAFCSLRNLLDG 383

>dbj|BAB02728.1| protein phosphatase 2C-like protein [Arabidopsis thaliana]
          Length = 422

 Score =  192 bits (489), Expect = 4e-48
 Identities = 101/172 (58%), Positives = 134/172 (77%), Gaps = 3/172 (1%)
 Frame = -2

Query: 836 KAVDMSHDHRPSYLPERRRVEELGGFIDDGYLNGYLSVTRALGDWDFK--LPIG-AASPL 666
           KAVDMS DH+ ++ PERRRVE+LGG+ +  YL G L+VTRALGDW  K   P+G + SPL
Sbjct: 259 KAVDMSFDHKSTFEPERRRVEDLGGYFEGEYLYGDLAVTRALGDWSIKRFSPLGESLSPL 318

Query: 665 IAEPDVQLITLTEDDEFLIIGCDGIWDVKYSQVSPVSLVRRGLRRHDDPQQCARELVKEA 486
           I++PD+Q + LTE+DEFLI+GCDG+WDV  SQ + V+ VR+GLRRH DP++CA EL +EA
Sbjct: 319 ISDPDIQQMILTEEDEFLIMGCDGVWDVMTSQYA-VTFVRQGLRRHGDPRRCAMELGREA 377

Query: 485 LRLNTSDNLTVIVICLSSIESTVESCPPQRRRFKACSLSEEARNRLKSLMEG 330
           LRL++SDN+TV+VIC SS      S  PQRRR + C +S+EAR RL++++EG
Sbjct: 378 LRLDSSDNVTVVVICFSS------SPAPQRRRIRFC-VSDEARARLQTMLEG 422

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 718,923,316
Number of Sequences: 1393205
Number of extensions: 15353751
Number of successful extensions: 38801
Number of sequences better than 10.0: 438
Number of HSP's better than 10.0 without gapping: 37084
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38415
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 43480044972
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB021f06_f BP035514 1 556 2 MR024h12_f BP077874 67 202 3 MR100c07_f BP083639 152 484 4 MPD060h06_f AV774051 276 696 5 SPD088f07_f BP051048 277 839

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