>KMC004262A_C01 KMC004262A_c01
tatcagagcgattaaattaataccaaattttaAAACATCAAAGAACAATTTAAATTCTAA
AAATGTTAAACTCTGATCTGATGATTGCACAAAAAGTTTAAAAAGGAAAATTATTCCCAT
TCCTTTTTCGGGGGAATTTTCCAGCTATGTAATTCCATATTTACTCCTCACCAAAAAAAA
ATCACTTTTGTTCCGTTCCAAACATGCTTCCGTTACCGCTACCGTTACCTTCCGGCGTCA
CCGTTCGGGAAAAATGGTCTAACTTGCTCTAACTATCCTCCATAACCTCCACCTCGCCGT
CACCGACGCCTTCCCCGGCGTCATCGCCGTCATCATCAAGCAACCGCTCCATGGGGGCAG
CACACTCCACCTTGTGCCGGAGCTTCCAATCCAGCGCCTGACACGCGCGCGAGCAGTAGT
TCACCGTGCCGCACACCGAGCACCGCCGGAACTCACGATTCCTCGTCTCCGGCCGACCGC
AGCCCATGTGCGAACAGAGACTTAACCCCGGCCCTGGAACATCATCCTTCGCCGCGAACC
ACTCAGTCATGAACCGGTTCG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004262A_C01 KMC004262A_c01
         (561 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_564894.1| F-box protein family; protein id: At1g67340.1, ...   128  5e-29
ref|NP_199856.1| putative protein; protein id: At5g50450.1, supp...   107  8e-23
dbj|BAB86182.1| OJ1485_B09.11 [Oryza sativa (japonica cultivar-g...    94  1e-18
gb|AAH47974.1| Similar to programmed cell death 2 [Xenopus laevis]     50  2e-05
ref|NP_496323.1| SET-domain transcriptional regulator like (48.5...    48  9e-05

>ref|NP_564894.1| F-box protein family; protein id: At1g67340.1, supported by cDNA:
           gi_16209725, supported by cDNA: gi_20260405 [Arabidopsis
           thaliana] gi|25373198|pir||H96696 protein F1N21.16
           [imported] - Arabidopsis thaliana
           gi|9828618|gb|AAG00241.1|AC002130_6 F1N21.16
           [Arabidopsis thaliana] gi|16209726|gb|AAL14418.1|
           At1g67340/F1N21_16 [Arabidopsis thaliana]
           gi|20260406|gb|AAM13101.1| unknown protein [Arabidopsis
           thaliana] gi|23197876|gb|AAN15465.1| unknown protein
           [Arabidopsis thaliana]
          Length = 379

 Score =  128 bits (321), Expect = 5e-29
 Identities = 59/85 (69%), Positives = 68/85 (79%), Gaps = 1/85 (1%)
 Frame = -3

Query: 559 NRFMTEWFAAKD-DVPGPGLSLCSHMGCGRPETRNREFRRCSVCGTVNYCSRACQALDWK 383
           NRF+ +WFA +  D PG GL LCSH GCGRPETR  EFRRCSVCG VNYCSRACQALDWK
Sbjct: 280 NRFLADWFAVRGGDCPGDGLRLCSHAGCGRPETRKHEFRRCSVCGVVNYCSRACQALDWK 339

Query: 382 LRHKVECAAPMERLLDDDGDDAGEG 308
           LRHK++C AP++R L++   D GEG
Sbjct: 340 LRHKMDC-APVQRWLEE--GDGGEG 361

>ref|NP_199856.1| putative protein; protein id: At5g50450.1, supported by cDNA:
           gi_20466553 [Arabidopsis thaliana]
           gi|9758927|dbj|BAB09464.1|
           gb|AAB95234.1~gene_id:MXI22.17~strong similarity to
           unknown protein [Arabidopsis thaliana]
           gi|20466554|gb|AAM20594.1| putative protein [Arabidopsis
           thaliana] gi|23198134|gb|AAN15594.1| putative protein
           [Arabidopsis thaliana]
          Length = 336

 Score =  107 bits (268), Expect = 8e-23
 Identities = 53/98 (54%), Positives = 62/98 (63%), Gaps = 7/98 (7%)
 Frame = -3

Query: 559 NRFMTEWFAAKDDVPGPGLSLCSHMGCGRPETRNREFRRCSVCGTVNYCSRACQALDWKL 380
           NRF+ EWF++       GL +CSH GCGRPETR  EFRRCSVCG VNYCSR CQALDW+ 
Sbjct: 240 NRFLKEWFSSGRVDLAEGLRMCSHGGCGRPETRAHEFRRCSVCGKVNYCSRGCQALDWRA 299

Query: 379 RHKVEC-------AAPMERLLDDDGDDAGEGVGDGEVE 287
           +HKVEC       AA  E  + DDG+     + D   E
Sbjct: 300 KHKVECTPLDLWVAAAAE--IGDDGEAVAVEIDDNHGE 335

>dbj|BAB86182.1| OJ1485_B09.11 [Oryza sativa (japonica cultivar-group)]
          Length = 388

 Score = 93.6 bits (231), Expect = 1e-18
 Identities = 47/93 (50%), Positives = 54/93 (57%), Gaps = 19/93 (20%)
 Frame = -3

Query: 559 NRFMTEWFAAK-------------------DDVPGPGLSLCSHMGCGRPETRNREFRRCS 437
           N FM +W+A++                   D   G  L LCSH+ CGR ETR  EFRRCS
Sbjct: 290 NLFMADWWASRGVQATAKKPGLEAPAAATGDSDGGGELRLCSHVRCGRRETRRHEFRRCS 349

Query: 436 VCGTVNYCSRACQALDWKLRHKVECAAPMERLL 338
           VCG  NYCSRACQALDWK  HK +C  PM+R L
Sbjct: 350 VCGAANYCSRACQALDWKRAHKAQC-VPMDRWL 381

>gb|AAH47974.1| Similar to programmed cell death 2 [Xenopus laevis]
          Length = 361

 Score = 49.7 bits (117), Expect = 2e-05
 Identities = 24/50 (48%), Positives = 29/50 (58%)
 Frame = -3

Query: 508 GLSLCSHMGCGRPETRNREFRRCSVCGTVNYCSRACQALDWKLRHKVECA 359
           GL LC   GC  P+T       CS C  VNYCS+  Q +DWKL+HK  C+
Sbjct: 132 GLRLCRVCGCLGPKT-------CSKCHKVNYCSKDHQLMDWKLQHKKVCS 174

>ref|NP_496323.1| SET-domain transcriptional regulator like (48.5 kD) [Caenorhabditis
           elegans] gi|3123316|sp|Q09415|YRM4_CAEEL Hypothetical
           protein R06F6.4 in chromosome II gi|7506368|pir||T23984
           hypothetical protein R06F6.4 - Caenorhabditis elegans
           gi|3878909|emb|CAA86783.1| Hypothetical protein R06F6.4
           [Caenorhabditis elegans]
          Length = 429

 Score = 47.8 bits (112), Expect = 9e-05
 Identities = 21/42 (50%), Positives = 26/42 (61%)
 Frame = -3

Query: 481 CGRPETRNREFRRCSVCGTVNYCSRACQALDWKLRHKVECAA 356
           C +  T   E ++CS C  + YCS+ CQ  DWKL HKVEC A
Sbjct: 26  CNQCLTSMAELKKCSACRRLAYCSQECQRADWKL-HKVECKA 66

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 534,123,798
Number of Sequences: 1393205
Number of extensions: 14381095
Number of successful extensions: 95841
Number of sequences better than 10.0: 786
Number of HSP's better than 10.0 without gapping: 66646
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89359
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20095422690
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR099b11_f BP083564 1 475 2 SPD093a01_f BP051386 33 253 3 MR021b12_f BP077580 53 514 4 MWM031a08_f AV765138 65 561

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