Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004258A_C01 KMC004258A_c01
(575 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_179032.1| putative methylmalonate semi-aldehyde dehydroge... 145 7e-35
pir||T02721 probable methylmalonate-semialdehyde dehydrogenase ... 144 8e-34
ref|NP_717289.1| methylmalonate-semialdehyde dehydrogenase [Shew... 131 5e-30
pir||T13418 methylmalonate-semialdehyde dehydrogenase (acylating... 128 6e-29
ref|NP_726672.1| CG17896-PA [Drosophila melanogaster] gi|4678960... 128 6e-29
>ref|NP_179032.1| putative methylmalonate semi-aldehyde dehydrogenase; protein id:
At2g14170.1 [Arabidopsis thaliana]
gi|25284289|pir||H84514 hypothetical protein At2g14170
[imported] - Arabidopsis thaliana
gi|4587685|gb|AAD25855.1| putative methylmalonate
semi-aldehyde dehydrogenase [Arabidopsis thaliana]
Length = 607
Score = 145 bits (367), Expect(2) = 7e-35
Identities = 67/88 (76%), Positives = 82/88 (93%)
Frame = -3
Query: 573 TILADVTADMECYKEEIFGPVLLLMESDSLEKAINIVNENKYGNGASIFTASGVSARKFQ 394
TIL+ VT DMECYKEEIFGPVL+ M+++S ++AI+I+N+NKYGNGA+IFT+SG +ARKFQ
Sbjct: 476 TILSGVTPDMECYKEEIFGPVLVCMQANSFDEAISIINKNKYGNGAAIFTSSGAAARKFQ 535
Query: 393 TEIEAGQVGINVPIPVPLPFFSFTGNKA 310
+IEAGQ+GINVPIPVPLPFFSFTGNKA
Sbjct: 536 MDIEAGQIGINVPIPVPLPFFSFTGNKA 563
Score = 66.6 bits (161), Expect = 2e-10
Identities = 31/59 (52%), Positives = 38/59 (63%)
Frame = -1
Query: 398 FRQRLRLGRWASMFPFLFLCPFSHLLATRPSFAGDLNFYGKAGVNFFTQIKTITQQWKE 222
F+ + G+ P PF + SFAGDLNFYGKAGV+FFTQIKT+TQQWK+
Sbjct: 534 FQMDIEAGQIGINVPIPVPLPFFSFTGNKASFAGDLNFYGKAGVDFFTQIKTVTQQWKD 592
Score = 23.1 bits (48), Expect(2) = 7e-35
Identities = 11/35 (31%), Positives = 21/35 (59%)
Frame = -2
Query: 277 RQGLTFLHRLKQ*LSSGRNQLVRTKINMAMPTSQK 173
+ G+ F ++K ++ + T +++AMPTSQK
Sbjct: 574 KAGVDFFTQIKTVTQQWKD--IPTSVSLAMPTSQK 606
>pir||T02721 probable methylmalonate-semialdehyde dehydrogenase (acylating) (EC
1.2.1.27) - rice gi|2895866|gb|AAC03055.1|
methylmalonate semi-aldehyde dehydrogenase [Oryza
sativa]
Length = 532
Score = 144 bits (363), Expect = 8e-34
Identities = 72/88 (81%), Positives = 80/88 (90%)
Frame = -3
Query: 573 TILADVTADMECYKEEIFGPVLLLMESDSLEKAINIVNENKYGNGASIFTASGVSARKFQ 394
T+LADV ++MECYKEEIFGPVLL M+++SL AI IVN NKYGNGASIFT SGVSARKFQ
Sbjct: 402 TLLADVKSEMECYKEEIFGPVLL-MKAESLNDAIQIVNRNKYGNGASIFTTSGVSARKFQ 460
Query: 393 TEIEAGQVGINVPIPVPLPFFSFTGNKA 310
T+IEAGQVGINVPIP PLPFFSFTG+KA
Sbjct: 461 TDIEAGQVGINVPIPEPLPFFSFTGSKA 488
Score = 68.9 bits (167), Expect = 4e-11
Identities = 33/63 (52%), Positives = 41/63 (64%)
Frame = -1
Query: 398 FRQRLRLGRWASMFPFLFLCPFSHLLATRPSFAGDLNFYGKAGVNFFTQIKTITQQWKES 219
F+ + G+ P PF ++ SFAGDLNFYGKAGV FFTQIKT+TQQWKES
Sbjct: 459 FQTDIEAGQVGINVPIPEPLPFFSFTGSKASFAGDLNFYGKAGVQFFTQIKTVTQQWKES 518
Query: 218 ASE 210
++
Sbjct: 519 PAQ 521
>ref|NP_717289.1| methylmalonate-semialdehyde dehydrogenase [Shewanella oneidensis
MR-1] gi|24347477|gb|AAN54733.1|AE015613_10
methylmalonate-semialdehyde dehydrogenase [Shewanella
oneidensis MR-1]
Length = 499
Score = 131 bits (330), Expect = 5e-30
Identities = 69/108 (63%), Positives = 78/108 (71%)
Frame = -3
Query: 573 TILADVTADMECYKEEIFGPVLLLMESDSLEKAINIVNENKYGNGASIFTASGVSARKFQ 394
T+ VT DM YKEEIFGPVL MESDSLE AI +VN + YGNG SIFTASG +ARK+Q
Sbjct: 367 TMFTKVTTDMSIYKEEIFGPVLCCMESDSLEDAIELVNASPYGNGTSIFTASGAAARKYQ 426
Query: 393 TEIEAGQVGINVPIPVPLPFFSFTGNKAIICWRSQFLWQGRG*LFYTD 250
EIE GQVGINVPIPVPLPFFSFTG K + Q + + FYT+
Sbjct: 427 HEIEVGQVGINVPIPVPLPFFSFTGWKGSF-YGDQHAYGKQAVRFYTE 473
Score = 43.5 bits (101), Expect = 0.002
Identities = 21/60 (35%), Positives = 31/60 (51%)
Frame = -1
Query: 398 FRQRLRLGRWASMFPFLFLCPFSHLLATRPSFAGDLNFYGKAGVNFFTQIKTITQQWKES 219
++ + +G+ P PF + SF GD + YGK V F+T+ KTIT +W ES
Sbjct: 425 YQHEIEVGQVGINVPIPVPLPFFSFTGWKGSFYGDQHAYGKQAVRFYTETKTITARWFES 484
>pir||T13418 methylmalonate-semialdehyde dehydrogenase (acylating) (EC 1.2.1.27)
- fruit fly (Drosophila melanogaster)
gi|2653397|emb|CAA15632.1|
/prediction=(method:''genefinder'', version:''084'',
score:''86.60'')~/prediction=(method:''genscan'',
version:''1.0'',
score:''210.16'')~/motif=(desc:''Aldehyde dehydrogenases
cysteine active site'', dbase:''PROSITE'',
acc:''PS00070'',
method:''ppsearch'')~/motif=(desc:''Crystallins beta and
gamma 'Greek key' motif signature'', dbase:''PROSITE'',
acc:''PS00225'',
method:''ppsearch'')~/match=(desc:''METHYLMALONATE-
SEMIALDEHYDE DEHYDROGENASE PRECURSOR [ACYLATING] (EC
1.2.1.27) (MMSDH)'', species:''RATTUS N>
Length = 552
Score = 128 bits (321), Expect = 6e-29
Identities = 63/108 (58%), Positives = 82/108 (75%), Gaps = 1/108 (0%)
Frame = -3
Query: 573 TILADVTADMECYKEEIFGPVLLLMESDSLEKAINIVNENKYGNGASIFTASGVSARKFQ 394
TIL+DVT M+CY EEIFGPVL+++++D+L+ AI IVN N YGNG ++FT +G +ARKF
Sbjct: 420 TILSDVTPSMKCYTEEIFGPVLVILKADTLDDAIGIVNANPYGNGTAVFTTNGAAARKFV 479
Query: 393 TEIEAGQVGINVPIPVPLPFFSFTGNKAIICWRSQFLWQGR-G*LFYT 253
EI+AGQVG+NVPIPVPLP FSFTG + +R + G+ G FYT
Sbjct: 480 NEIDAGQVGVNVPIPVPLPMFSFTGTRG--SFRGDHHFYGKQGIKFYT 525
Score = 49.3 bits (116), Expect = 3e-05
Identities = 20/40 (50%), Positives = 27/40 (67%)
Frame = -1
Query: 338 PFSHLLATRPSFAGDLNFYGKAGVNFFTQIKTITQQWKES 219
P TR SF GD +FYGK G+ F+TQ KT+TQ W+++
Sbjct: 498 PMFSFTGTRGSFRGDHHFYGKQGIKFYTQTKTVTQLWRKT 537
>ref|NP_726672.1| CG17896-PA [Drosophila melanogaster] gi|4678960|emb|CAB41309.1|
alternatively spliced
form~/prediction=(method:''genefinder'',
version:''084'')~/prediction=(method:''genscan'',
version:''1.0'')~/match=(desc:''METHYLMALONATE-
SEMIALDEHYDE DEHYDROGENASE PRECURSOR [ACYLATING] (EC
1.2.1.27) (MMSDH)'', species:''RATTUS NORVEGICUS
(RAT)'', ranges:(query:14228..15727,
target:SWISS-PROT::Q02253:533..34, score:''1893.00'')),
method:''blastx'',
version:''1.4.9'')~/match=(desc:''GM07535.5prime GM
Drosophila melanogaster ovary BlueScript Drosophila
melanogaster cDNA clone GM07535 >
gi|7290044|gb|AAF45511.1| CG17896-PA [Drosophila
melanogaster]
Length = 511
Score = 128 bits (321), Expect = 6e-29
Identities = 63/108 (58%), Positives = 82/108 (75%), Gaps = 1/108 (0%)
Frame = -3
Query: 573 TILADVTADMECYKEEIFGPVLLLMESDSLEKAINIVNENKYGNGASIFTASGVSARKFQ 394
TIL+DVT M+CY EEIFGPVL+++++D+L+ AI IVN N YGNG ++FT +G +ARKF
Sbjct: 379 TILSDVTPSMKCYTEEIFGPVLVILKADTLDDAIGIVNANPYGNGTAVFTTNGAAARKFV 438
Query: 393 TEIEAGQVGINVPIPVPLPFFSFTGNKAIICWRSQFLWQGR-G*LFYT 253
EI+AGQVG+NVPIPVPLP FSFTG + +R + G+ G FYT
Sbjct: 439 NEIDAGQVGVNVPIPVPLPMFSFTGTRG--SFRGDHHFYGKQGIKFYT 484
Score = 49.3 bits (116), Expect = 3e-05
Identities = 20/40 (50%), Positives = 27/40 (67%)
Frame = -1
Query: 338 PFSHLLATRPSFAGDLNFYGKAGVNFFTQIKTITQQWKES 219
P TR SF GD +FYGK G+ F+TQ KT+TQ W+++
Sbjct: 457 PMFSFTGTRGSFRGDHHFYGKQGIKFYTQTKTVTQLWRKT 496
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 474,224,453
Number of Sequences: 1393205
Number of extensions: 9829332
Number of successful extensions: 23295
Number of sequences better than 10.0: 1235
Number of HSP's better than 10.0 without gapping: 22507
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23239
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21530810025
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
