Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004235A_C01 KMC004235A_c01
(548 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC53893.1| cytochrome P450 [Petunia x hybrida] 65 5e-19
gb|AAM74256.1|AC074355_18 Putative cytochrome P450 [Oryza sativa... 77 9e-19
dbj|BAC42682.1| putative cytochrome P450 [Arabidopsis thaliana] 64 5e-18
ref|NP_190865.1| cytochrome p450 family; protein id: At3g52970.1... 64 5e-18
dbj|BAC10997.1| flavonoid 3',5'-hydroxylase [Nierembergia sp. NB17] 58 1e-17
>dbj|BAC53893.1| cytochrome P450 [Petunia x hybrida]
Length = 532
Score = 64.7 bits (156), Expect(2) = 5e-19
Identities = 27/52 (51%), Positives = 41/52 (77%)
Frame = -2
Query: 400 DYELIPFGAGRRMCAGVPLAHRILHLFLGSLLHRFDWELDSSVTRSTIDMRD 245
D+ELIPFGAGRR+C G+PLA R++ + LGSLL+ F+W+L++ + +DM +
Sbjct: 425 DFELIPFGAGRRICPGLPLALRMVPVVLGSLLNSFNWKLETGIEPEELDMEE 476
Score = 50.8 bits (120), Expect(2) = 5e-19
Identities = 23/44 (52%), Positives = 28/44 (63%)
Frame = -1
Query: 536 GTSYPKTHKSLVNTWAIGRDPDVWDEPLCFKPESFFDGKSDYQG 405
G PK + LVN WAIGRD W++PL FKPE F++ D QG
Sbjct: 380 GYIVPKGSQVLVNVWAIGRDSTYWEDPLMFKPERFWNLDLDVQG 423
>gb|AAM74256.1|AC074355_18 Putative cytochrome P450 [Oryza sativa (japonica cultivar-group)]
Length = 433
Score = 76.6 bits (187), Expect(2) = 9e-19
Identities = 31/52 (59%), Positives = 43/52 (82%)
Frame = -2
Query: 400 DYELIPFGAGRRMCAGVPLAHRILHLFLGSLLHRFDWELDSSVTRSTIDMRD 245
D++LIPFGAG+R+C G+PLAHR++HL LGSLLHRF W L + ++ +DMR+
Sbjct: 362 DFQLIPFGAGKRICLGLPLAHRMVHLMLGSLLHRFTWTLPAEAGKNGVDMRE 413
Score = 38.1 bits (87), Expect(2) = 9e-19
Identities = 16/36 (44%), Positives = 20/36 (55%)
Frame = -1
Query: 512 KSLVNTWAIGRDPDVWDEPLCFKPESFFDGKSDYQG 405
K L+N WAI R + W EP F PE F+D + G
Sbjct: 325 KVLINLWAINRCANTWTEPDKFMPERFYDSDITFMG 360
>dbj|BAC42682.1| putative cytochrome P450 [Arabidopsis thaliana]
Length = 516
Score = 63.9 bits (154), Expect(3) = 5e-18
Identities = 26/57 (45%), Positives = 39/57 (67%)
Frame = -2
Query: 409 KVHDYELIPFGAGRRMCAGVPLAHRILHLFLGSLLHRFDWELDSSVTRSTIDMRDNL 239
K DYE +PFG+GRRMC +PLA R+L L +GS++ FDW L++ + +DM + +
Sbjct: 440 KGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERI 496
Score = 47.4 bits (111), Expect(3) = 5e-18
Identities = 21/42 (50%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Frame = -1
Query: 524 PKTHKSLVNTWAIGRDPDVWDEPLCFKPESFFDGKS--DYQG 405
PK + LVN WAIGRDP W +P+ FKPE F D++G
Sbjct: 400 PKETQVLVNVWAIGRDPKTWVDPIMFKPERFISDPDARDFKG 441
Score = 20.8 bits (42), Expect(3) = 5e-18
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = -3
Query: 237 GVTMRKLEPLLAVP 196
G+T++K PL A+P
Sbjct: 497 GITLKKAVPLEAIP 510
>ref|NP_190865.1| cytochrome p450 family; protein id: At3g52970.1 [Arabidopsis
thaliana] gi|11249509|pir||T47554 cytochrome P450
homolog F8J2.140 [similarity] - Arabidopsis thaliana
gi|7529721|emb|CAB86901.1| cytochrome P450-like protein
[Arabidopsis thaliana]
Length = 512
Score = 63.9 bits (154), Expect(3) = 5e-18
Identities = 26/57 (45%), Positives = 39/57 (67%)
Frame = -2
Query: 409 KVHDYELIPFGAGRRMCAGVPLAHRILHLFLGSLLHRFDWELDSSVTRSTIDMRDNL 239
K DYE +PFG+GRRMC +PLA R+L L +GS++ FDW L++ + +DM + +
Sbjct: 436 KGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERI 492
Score = 47.4 bits (111), Expect(3) = 5e-18
Identities = 21/42 (50%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Frame = -1
Query: 524 PKTHKSLVNTWAIGRDPDVWDEPLCFKPESFFDGKS--DYQG 405
PK + LVN WAIGRDP W +P+ FKPE F D++G
Sbjct: 396 PKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDARDFKG 437
Score = 20.8 bits (42), Expect(3) = 5e-18
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = -3
Query: 237 GVTMRKLEPLLAVP 196
G+T++K PL A+P
Sbjct: 493 GITLKKAVPLEAIP 506
>dbj|BAC10997.1| flavonoid 3',5'-hydroxylase [Nierembergia sp. NB17]
Length = 503
Score = 58.2 bits (139), Expect(2) = 1e-17
Identities = 25/51 (49%), Positives = 37/51 (72%)
Frame = -2
Query: 424 ARVITKVHDYELIPFGAGRRMCAGVPLAHRILHLFLGSLLHRFDWELDSSV 272
A++ + +D+ELIPFGAGRR+CAG + ++ LG+L+H FDW+L S V
Sbjct: 419 AKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSDV 469
Score = 53.1 bits (126), Expect(2) = 1e-17
Identities = 23/39 (58%), Positives = 25/39 (63%)
Frame = -1
Query: 536 GTSYPKTHKSLVNTWAIGRDPDVWDEPLCFKPESFFDGK 420
G PK + VN WAIGRDPDVW+ PL F PE F GK
Sbjct: 379 GYYIPKNTRLSVNIWAIGRDPDVWENPLKFNPERFLSGK 417
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 482,275,750
Number of Sequences: 1393205
Number of extensions: 10173178
Number of successful extensions: 23846
Number of sequences better than 10.0: 1652
Number of HSP's better than 10.0 without gapping: 21523
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23826
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 18947112822
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
