Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004223A_C01 KMC004223A_c01
(668 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC16386.1| putative centromere/microtubule binding protein ... 77 3e-13
ref|NP_191274.1| putative protein; protein id: At3g57150.1, supp... 72 7e-12
gb|AAG46137.1|AC082644_19 putative centromere/microtubule bindin... 72 9e-12
pir||T06379 SAR DNA-binding protein 2 - garden pea gi|3132698|gb... 69 6e-11
ref|NP_500057.1| Putative nuclear protein, nematode specific [Ca... 69 7e-11
>dbj|BAC16386.1| putative centromere/microtubule binding protein [Oryza sativa
(japonica cultivar-group)]
Length = 600
Score = 76.6 bits (187), Expect = 3e-13
Identities = 54/117 (46%), Positives = 66/117 (56%), Gaps = 4/117 (3%)
Frame = -1
Query: 665 ASVPAKKVKVDVVVDEGKVKVEVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHK 486
ASVP KK+KV+ +E VEGEK EKKKKKKKD+ GE S D E K+ K
Sbjct: 495 ASVPVKKIKVEESAEE------VEGEKSEKKKKKKKDK--GEQGSGDAE------VKEEK 540
Query: 485 VKVEDGSPDLDQSEKKKKKKKDQDAED----NAAEISNGKEDRKSEKKHKKKKNPGR 327
+ DG + SEKKKKKKK ++ D +A +G E KSEKK +KKK R
Sbjct: 541 ADISDG--EKVGSEKKKKKKKSKEGGDVVDPESAANGDGVEAEKSEKKKEKKKKKSR 595
Score = 61.2 bits (147), Expect = 1e-08
Identities = 47/132 (35%), Positives = 70/132 (52%), Gaps = 19/132 (14%)
Frame = -1
Query: 668 PASVPAKKVKVDVVVDEGKVKVE-----VEGEKKEKKKKKKKDQEN----GEVASSDEEK 516
P A + +E KVK E E K++KKKKK KD+E+ G +++
Sbjct: 433 PTGGDAMIASIAAAPEEAKVKAEQDVVATEEVKEKKKKKKHKDEEDAADEGRKRKIEDDD 492
Query: 515 AEKEKKKKHKVKVEDGSPDLD--QSEKKKKKKKDQ------DAE--DNAAEISNGKEDRK 366
A K K+KVE+ + +++ +SEKKKKKKKD+ DAE + A+IS+G++
Sbjct: 493 ASASVPVK-KIKVEESAEEVEGEKSEKKKKKKKDKGEQGSGDAEVKEEKADISDGEKVGS 551
Query: 365 SEKKHKKKKNPG 330
+KK KKK G
Sbjct: 552 EKKKKKKKSKEG 563
Score = 45.8 bits (107), Expect = 5e-04
Identities = 37/99 (37%), Positives = 52/99 (52%), Gaps = 11/99 (11%)
Frame = -1
Query: 584 KEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKVEDGSPDLDQSEKKKKKKKDQDA-- 411
+E K K ++D + EE EK+KKKKHK + ED + E +K+K +D DA
Sbjct: 448 EEAKVKAEQD------VVATEEVKEKKKKKKHKDE-EDAA-----DEGRKRKIEDDDASA 495
Query: 410 ---------EDNAAEISNGKEDRKSEKKHKKKKNPGRRG 321
E++A E+ E KSEKK KKKK+ G +G
Sbjct: 496 SVPVKKIKVEESAEEV----EGEKSEKKKKKKKDKGEQG 530
>ref|NP_191274.1| putative protein; protein id: At3g57150.1, supported by cDNA:
gi_8901185 [Arabidopsis thaliana]
gi|11357617|pir||T47775 hypothetical protein F24I3.230 -
Arabidopsis thaliana gi|6911885|emb|CAB72185.1| putative
protein [Arabidopsis thaliana]
gi|8901186|gb|AAF43210.2|AF234984_1 putative
pseudouridine synthase [Arabidopsis thaliana]
gi|28392852|gb|AAO41863.1| unknown protein [Arabidopsis
thaliana]
Length = 565
Score = 72.0 bits (175), Expect = 7e-12
Identities = 47/112 (41%), Positives = 61/112 (53%), Gaps = 1/112 (0%)
Frame = -1
Query: 668 PASVPAKKVKVDVVVDEGKVKVEVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKH 489
PA V KK K V E E E + K KKKKKKD+E + + EK EK+KKK
Sbjct: 451 PAPVTTKKSKTKEVEGE-----EAEEKVKSSKKKKKKDKEEEKEEEAGSEKKEKKKKKDK 505
Query: 488 KVKVEDGSPDLDQSEKKKKKKKDQDAEDNAAEISNG-KEDRKSEKKHKKKKN 336
K +V + +KKKKK KD +A +A + S K ++K +KK KKKKN
Sbjct: 506 KEEVIEEVASPKSEKKKKKKSKDTEAAVDAEDESAAEKSEKKKKKKDKKKKN 557
Score = 53.9 bits (128), Expect = 2e-06
Identities = 43/92 (46%), Positives = 55/92 (59%), Gaps = 9/92 (9%)
Frame = -1
Query: 650 KKVKVDVVVDEGKVKVEVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHK----- 486
KK K D E + + E EKKEKKKKK K +E E +S K+EK+KKKK K
Sbjct: 478 KKKKKD---KEEEKEEEAGSEKKEKKKKKDKKEEVIEEVASP--KSEKKKKKKSKDTEAA 532
Query: 485 VKVEDGSPDLDQSEKKK----KKKKDQDAEDN 402
V ED S ++SEKKK KKKK++D+ED+
Sbjct: 533 VDAEDESA-AEKSEKKKKKKDKKKKNKDSEDD 563
Score = 44.7 bits (104), Expect = 0.001
Identities = 34/94 (36%), Positives = 47/94 (49%), Gaps = 2/94 (2%)
Frame = -1
Query: 614 KVKVEVE-GEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHK-VKVEDGSPDLDQSEK 441
++K + E GE E +K+K D SSD KK K K V+ E+ + S+K
Sbjct: 426 EIKADAENGEAGEARKRKHDD-------SSDSPAPVTTKKSKTKEVEGEEAEEKVKSSKK 478
Query: 440 KKKKKKDQDAEDNAAEISNGKEDRKSEKKHKKKK 339
KKKK K+++ E+ A SEKK KKKK
Sbjct: 479 KKKKDKEEEKEEEAG----------SEKKEKKKK 502
>gb|AAG46137.1|AC082644_19 putative centromere/microtubule binding protein [Oryza sativa]
Length = 591
Score = 71.6 bits (174), Expect = 9e-12
Identities = 50/96 (52%), Positives = 63/96 (65%), Gaps = 10/96 (10%)
Frame = -1
Query: 665 ASVPAKKVKVDVVVDEGKVKVEVEGEKKEKKKKKKKDQ-----ENGEVAS--SDEEK--A 513
ASV AKK+KV+ D VEGEK EKKKKKKKD+ +GEV + SD EK +
Sbjct: 499 ASVSAKKIKVEEEAD------AVEGEKSEKKKKKKKDKAESAYADGEVKAELSDGEKGGS 552
Query: 512 EKEKKKKHKVKVEDGSPDLDQSEKKK-KKKKDQDAE 408
EK+KKKK + E G + ++SEKKK KKKK++DAE
Sbjct: 553 EKKKKKKKSKEGEAGDDEAEKSEKKKEKKKKNRDAE 588
Score = 61.2 bits (147), Expect = 1e-08
Identities = 41/109 (37%), Positives = 58/109 (52%), Gaps = 11/109 (10%)
Frame = -1
Query: 614 KVKVEVEGEKKEKKKKKKKD------QENGEVASSDEEKAEKEKKKKHKVKVEDGSPDLD 453
K + +V E KEKKKKK KD E + D++ + KK KV+ E + + +
Sbjct: 459 KEEADVAEETKEKKKKKHKDWAGDNADEGRKRKVGDDDLSASVSAKKIKVEEEADAVEGE 518
Query: 452 QSEKKKKKKKDQDAEDNA-----AEISNGKEDRKSEKKHKKKKNPGRRG 321
+SEKKKKKKKD+ A AE+S+G++ +KK KKK G G
Sbjct: 519 KSEKKKKKKKDKAESAYADGEVKAELSDGEKGGSEKKKKKKKSKEGEAG 567
>pir||T06379 SAR DNA-binding protein 2 - garden pea gi|3132698|gb|AAC16331.1|
SAR DNA-binding protein-2 [Pisum sativum]
Length = 550
Score = 68.9 bits (167), Expect = 6e-11
Identities = 44/110 (40%), Positives = 63/110 (57%), Gaps = 9/110 (8%)
Frame = -1
Query: 641 KVDVVVDEGKVKVEVEGEKKEKKKKKKKDQENGEVASSDEE------KAEKEKKKKH--- 489
K + +DE + V G+KKEKK+KKK++++ +V EE K EK+KKKK
Sbjct: 446 KSNSAMDEDTPETPVTGKKKEKKEKKKREKKEEKVEDVVEEPEQEVVKKEKKKKKKESTE 505
Query: 488 KVKVEDGSPDLDQSEKKKKKKKDQDAEDNAAEISNGKEDRKSEKKHKKKK 339
K +V++G +L+ EKKKK+KK + ED D S+KK KKKK
Sbjct: 506 KAEVQNGDNNLNGGEKKKKRKKSAEEEDTT--------DMPSKKKEKKKK 547
Score = 57.4 bits (137), Expect = 2e-07
Identities = 36/78 (46%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = -1
Query: 650 KKVKVDVVVDEGKVKVEVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKV-E 474
K+ KV+ VV+E E E KKEKKKKKK+ E EV + D EKKKK K E
Sbjct: 476 KEEKVEDVVEEP----EQEVVKKEKKKKKKESTEKAEVQNGDNNLNGGEKKKKRKKSAEE 531
Query: 473 DGSPDLDQSEKKKKKKKD 420
+ + D+ +K+KKKKKD
Sbjct: 532 EDTTDMPSKKKEKKKKKD 549
Score = 51.6 bits (122), Expect = 9e-06
Identities = 29/83 (34%), Positives = 44/83 (52%), Gaps = 2/83 (2%)
Frame = -1
Query: 581 EKKKKKKKDQENGE--VASSDEEKAEKEKKKKHKVKVEDGSPDLDQSEKKKKKKKDQDAE 408
E K D++ E V +EK EK+K++K + KVED + +Q KK+KKK +
Sbjct: 444 EPKSNSAMDEDTPETPVTGKKKEKKEKKKREKKEEKVEDVVEEPEQEVVKKEKKKKKKES 503
Query: 407 DNAAEISNGKEDRKSEKKHKKKK 339
AE+ NG + +K KK+K
Sbjct: 504 TEKAEVQNGDNNLNGGEKKKKRK 526
Score = 32.3 bits (72), Expect = 5.9
Identities = 27/114 (23%), Positives = 50/114 (43%), Gaps = 11/114 (9%)
Frame = -1
Query: 620 EGKVKVEVEGEKKEKKK-----KKKKDQENGEV--ASSDEEKAEKEKKKKHKVKVEDGSP 462
EGK G K K K K KK G + A + A+ + K +++ +P
Sbjct: 397 EGKELGHFAGSAKGKPKIEAYDKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDTP 456
Query: 461 DLD----QSEKKKKKKKDQDAEDNAAEISNGKEDRKSEKKHKKKKNPGRRGRIK 312
+ + EKK+KKK+++ E + +++ ++K KKKK + ++
Sbjct: 457 ETPVTGKKKEKKEKKKREKKEEKVEDVVEEPEQEVVKKEKKKKKKESTEKAEVQ 510
>ref|NP_500057.1| Putative nuclear protein, nematode specific [Caenorhabditis
elegans] gi|25395985|pir||G88636 protein W09G12.7
[imported] - Caenorhabditis elegans
gi|2911882|gb|AAC04447.1| Hypothetical protein W09G12.7
[Caenorhabditis elegans]
Length = 368
Score = 68.6 bits (166), Expect = 7e-11
Identities = 47/130 (36%), Positives = 71/130 (54%), Gaps = 21/130 (16%)
Frame = -1
Query: 656 PAKKVKVDVVVDEGKVKVEVEGEKKEKKKKKKKDQENGEVAS--------SDEEKAEKEK 501
PA+ +VVV VK + +G++ +K KK KK QE+ + +S SD++K K+K
Sbjct: 44 PAESSDDEVVVVV--VKKDQKGKRGKKSKKPKKKQESSDDSSDNSDSSESSDDDKKSKKK 101
Query: 500 KKKHKVKVEDGSPDLDQSE------KKKKKKKDQDAEDNAAEISNGK-------EDRKSE 360
KKK K D S D S+ KKKKKK +D+ D++++ S+ +D+K +
Sbjct: 102 KKKSKKDSSDSSDSSDSSDSSDDGKKKKKKKSKKDSSDDSSDSSDSSDSSDSSDDDKKKK 161
Query: 359 KKHKKKKNPG 330
KK KK KN G
Sbjct: 162 KKSKKSKNKG 171
Score = 56.6 bits (135), Expect = 3e-07
Identities = 31/102 (30%), Positives = 51/102 (49%), Gaps = 13/102 (12%)
Frame = -1
Query: 590 EKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKVEDGSPDL-------------DQ 450
+ K+KKKK KKD + +S + ++ KKKK K +D S D D
Sbjct: 97 KSKKKKKKSKKDSSDSSDSSDSSDSSDDGKKKKKKKSKKDSSDDSSDSSDSSDSSDSSDD 156
Query: 449 SEKKKKKKKDQDAEDNAAEISNGKEDRKSEKKHKKKKNPGRR 324
+KKKKK K + ++++ S+ +D ++K KKKK ++
Sbjct: 157 DKKKKKKSKKSKNKGSSSDSSDSSDDSSDDEKPKKKKKKSKK 198
Score = 49.7 bits (117), Expect = 4e-05
Identities = 40/110 (36%), Positives = 55/110 (49%), Gaps = 15/110 (13%)
Frame = -1
Query: 596 EGEKKEKKKKKKKDQENGEVAS--------SDEEKAEKEKKKKHKVK-----VEDGSPDL 456
+G+KK+KKK KK ++ +S SD++K +K+K KK K K D S D
Sbjct: 124 DGKKKKKKKSKKDSSDDSSDSSDSSDSSDSSDDDKKKKKKSKKSKNKGSSSDSSDSSDDS 183
Query: 455 DQSEKKKKKKKDQDAEDNAAEISNGKEDRKSEKKHKKKKN--PGRRGRIK 312
EK KKKKK + + A S+ + K KK KKK RR R+K
Sbjct: 184 SDDEKPKKKKK-KSKKPKKAPKSDEEVTIKIIKKPKKKPEDLERRRHRVK 232
Score = 46.2 bits (108), Expect = 4e-04
Identities = 27/93 (29%), Positives = 47/93 (50%)
Frame = -1
Query: 602 EVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKVEDGSPDLDQSEKKKKKKK 423
E + +KKK KK +E +V + + AE + V V+ + +K KK KK
Sbjct: 16 ETHKSESKKKKDKKTFEEEDDVENVKRKPAESSDDEVVVVVVKKDQKG-KRGKKSKKPKK 74
Query: 422 DQDAEDNAAEISNGKEDRKSEKKHKKKKNPGRR 324
Q++ D++++ S+ E +KK KKKK ++
Sbjct: 75 KQESSDDSSDNSDSSESSDDDKKSKKKKKKSKK 107
Score = 44.3 bits (103), Expect = 0.002
Identities = 27/85 (31%), Positives = 38/85 (43%), Gaps = 4/85 (4%)
Frame = -1
Query: 590 EKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKVEDGSPDLDQSEK----KKKKKK 423
+KK KK K K + +S D EK KKKK K K +P D+ KK KKK
Sbjct: 161 KKKSKKSKNKGSSSDSSDSSDDSSDDEKPKKKKKKSKKPKKAPKSDEEVTIKIIKKPKKK 220
Query: 422 DQDAEDNAAEISNGKEDRKSEKKHK 348
+D E + +E + + K +
Sbjct: 221 PEDLERRRHRVKKTEEHEEEKDKEE 245
Score = 43.9 bits (102), Expect = 0.002
Identities = 28/96 (29%), Positives = 48/96 (49%), Gaps = 11/96 (11%)
Frame = -1
Query: 590 EKKEKKKKKKKDQENGEVA--------SSDEEKAEKEKKKKHKVK---VEDGSPDLDQSE 444
+ K+KKKK KK + G + SSD+EK +K+KKK K K D + +
Sbjct: 156 DDKKKKKKSKKSKNKGSSSDSSDSSDDSSDDEKPKKKKKKSKKPKKAPKSDEEVTIKIIK 215
Query: 443 KKKKKKKDQDAEDNAAEISNGKEDRKSEKKHKKKKN 336
K KKK +D + + + + E+ K +++H + +
Sbjct: 216 KPKKKPEDLERRRHRVKKTEEHEEEKDKEEHHESND 251
Score = 40.4 bits (93), Expect = 0.022
Identities = 29/123 (23%), Positives = 53/123 (42%), Gaps = 18/123 (14%)
Frame = -1
Query: 650 KKVKVDVVVDEGKVKVEVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKVED 471
KK K + +V ++ KK KKK + ++ V ++E + EK+K++ H+ E+
Sbjct: 194 KKSKKPKKAPKSDEEVTIKIIKKPKKKPEDLERRRHRVKKTEEHEEEKDKEEHHESNDEN 253
Query: 470 -----------GSPDLDQSEKKKKKKKDQ-------DAEDNAAEISNGKEDRKSEKKHKK 345
SP L + K+ + D+ + +DN +G D E+ K
Sbjct: 254 MEFDTRARGAPSSPKLTRRAKRSESSDDEVELQKTPEIDDNKPSRKDGDHDSDMEESEKS 313
Query: 344 KKN 336
KK+
Sbjct: 314 KKS 316
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 573,769,369
Number of Sequences: 1393205
Number of extensions: 13431291
Number of successful extensions: 205143
Number of sequences better than 10.0: 7417
Number of HSP's better than 10.0 without gapping: 91366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155352
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 29138478756
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
