>KMC004195A_C01 KMC004195A_c01
gctgtaaatggtttaaattattcaatttaatagaaactcataaactaaccattaagagat
atgacacaattggctgagaaTTTTCGATCGATAGAACATCTTAAGAATTAGACCTTTTAG
AAATAGCCTCAAAAAGAAAAACTAGCCTTTGTTGGCTATCATGAGTCTATATTGGAGTAC
CTCAGTGATGATGAGGACGATAATAATGGGGTAAATGATGTGGCATGAGCCTTATTGTAT
GTATGAGGCCTTTAATGTAAGAAATCAAGTTTTTGCTTGCGTGCATGCCTTGTCTCTTTT
TGCATAATTGATCCTCATTCTCCCTCTTCATTTCTTCTTGTGTTCTGTCTTTATTTAACC
AAATGTAGCACCAGAAGGTTACATGATATTTTAACCAAATGTAGTTTCCAATTCCAAAAC
TCAACACCTTGATGAATTATCAGAACTAAATTCTCCACTTTCATCGATGATGGAGATTCA
ACATGGAACCGAATTATTGCAGGCTCTGCTTTATTCTTTTATTATGATCCACATATTCAA
ACACAACTGCAACACTATCAAACATGGATTCCATTAGTCTAACTCAAGCATACGACTCAT
CAACATTACACTCCCCGATATAAACCTTGATGCTCCGCGGTGAACCGCAGCTGCCTTCGG
CAATAAGTGCTCTGTCATTTGCAGCCAGTGTGAGCAATAATTTGGTAAATCATTTCAATA
TCTGGTGGAGCATGGCAACGCTCAGCTACTTCTTCAAGTGTCAAGGGCTCGACAGGCTCT
TGGCAGCTGGCCTCATTTAGCACGGCCAATACTCGTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004195A_C01 KMC004195A_c01
         (817 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAN41353.1| putative glucose-6-phosphate isomerase [Arabidops...    76  3e-26
gb|AAF24124.1|AF120494_1 phosphoglucose isomerase precursor [Ara...    76  3e-26
ref|NP_194193.1| glucose-6-phosphate isomerase; protein id: At4g...    76  3e-26
pir||T09153 glucose-6-phosphate isomerase (EC 5.3.1.9) precursor...    76  2e-25
ref|ZP_00071859.1| hypothetical protein [Trichodesmium erythraeu...    30  0.45

>gb|AAN41353.1| putative glucose-6-phosphate isomerase [Arabidopsis thaliana]
          Length = 613

 Score = 75.9 bits (185), Expect(2) = 3e-26
 Identities = 33/42 (78%), Positives = 41/42 (97%)
 Frame = -3

Query: 815 RVLAVLNEASCQEPVEPLTLEEVAERCHAPPDIEMIYQIIAH 690
           RVL+VLNEA+C++PVEPLTLEE+A+RCHAP +IEMIY+IIAH
Sbjct: 538 RVLSVLNEATCKDPVEPLTLEEIADRCHAPEEIEMIYKIIAH 579

 Score = 65.5 bits (158), Expect(2) = 3e-26
 Identities = 28/39 (71%), Positives = 35/39 (88%)
 Frame = -1

Query: 703 KLLLTLAANDRALIAEGSCGSPRSIKVYIGECNVDESYA 587
           K++  ++ANDR LIAEG+CGSPRSIKVY+GECNVD+ YA
Sbjct: 575 KIIAHMSANDRVLIAEGNCGSPRSIKVYLGECNVDDLYA 613

>gb|AAF24124.1|AF120494_1 phosphoglucose isomerase precursor [Arabidopsis thaliana]
          Length = 612

 Score = 75.9 bits (185), Expect(2) = 3e-26
 Identities = 33/42 (78%), Positives = 41/42 (97%)
 Frame = -3

Query: 815 RVLAVLNEASCQEPVEPLTLEEVAERCHAPPDIEMIYQIIAH 690
           RVL+VLNEA+C++PVEPLTLEE+A+RCHAP +IEMIY+IIAH
Sbjct: 537 RVLSVLNEATCKDPVEPLTLEEIADRCHAPEEIEMIYKIIAH 578

 Score = 65.5 bits (158), Expect(2) = 3e-26
 Identities = 28/39 (71%), Positives = 35/39 (88%)
 Frame = -1

Query: 703 KLLLTLAANDRALIAEGSCGSPRSIKVYIGECNVDESYA 587
           K++  ++ANDR LIAEG+CGSPRSIKVY+GECNVD+ YA
Sbjct: 574 KIIAHMSANDRVLIAEGNCGSPRSIKVYLGECNVDDLYA 612

>ref|NP_194193.1| glucose-6-phosphate isomerase; protein id: At4g24620.1 [Arabidopsis
           thaliana] gi|7484979|pir||T05572 glucose-6-phosphate
           isomerase (EC 5.3.1.9) - Arabidopsis thaliana
           gi|4220528|emb|CAA23001.1| glucose-6-phosphate isomerase
           [Arabidopsis thaliana] gi|7269312|emb|CAB79372.1|
           glucose-6-phosphate isomerase [Arabidopsis thaliana]
          Length = 611

 Score = 75.9 bits (185), Expect(2) = 3e-26
 Identities = 33/42 (78%), Positives = 41/42 (97%)
 Frame = -3

Query: 815 RVLAVLNEASCQEPVEPLTLEEVAERCHAPPDIEMIYQIIAH 690
           RVL+VLNEA+C++PVEPLTLEE+A+RCHAP +IEMIY+IIAH
Sbjct: 536 RVLSVLNEATCKDPVEPLTLEEIADRCHAPEEIEMIYKIIAH 577

 Score = 65.5 bits (158), Expect(2) = 3e-26
 Identities = 28/39 (71%), Positives = 35/39 (88%)
 Frame = -1

Query: 703 KLLLTLAANDRALIAEGSCGSPRSIKVYIGECNVDESYA 587
           K++  ++ANDR LIAEG+CGSPRSIKVY+GECNVD+ YA
Sbjct: 573 KIIAHMSANDRVLIAEGNCGSPRSIKVYLGECNVDDLYA 611

>pir||T09153 glucose-6-phosphate isomerase (EC 5.3.1.9) precursor, chloroplast -
           spinach gi|3413511|emb|CAA03982.1| glucose-6-phosphate
           isomerase [Spinacia oleracea]
          Length = 618

 Score = 75.9 bits (185), Expect(2) = 2e-25
 Identities = 34/42 (80%), Positives = 39/42 (91%)
 Frame = -3

Query: 815 RVLAVLNEASCQEPVEPLTLEEVAERCHAPPDIEMIYQIIAH 690
           RVLAVLNEASC++PVEPLT+EEVA+ CH P DIEMIY+IIAH
Sbjct: 543 RVLAVLNEASCKDPVEPLTIEEVADHCHCPDDIEMIYKIIAH 584

 Score = 62.8 bits (151), Expect(2) = 2e-25
 Identities = 26/39 (66%), Positives = 33/39 (83%)
 Frame = -1

Query: 703 KLLLTLAANDRALIAEGSCGSPRSIKVYIGECNVDESYA 587
           K++  +AANDR ++AEG CGSPRSIK ++GECNVDE YA
Sbjct: 580 KIIAHMAANDRVILAEGDCGSPRSIKAFLGECNVDELYA 618

>ref|ZP_00071859.1| hypothetical protein [Trichodesmium erythraeum IMS101]
          Length = 528

 Score = 30.4 bits (67), Expect(2) = 0.45
 Identities = 13/26 (50%), Positives = 19/26 (73%)
 Frame = -3

Query: 767 PLTLEEVAERCHAPPDIEMIYQIIAH 690
           PL+LEE++E+  A   IE IY+I+ H
Sbjct: 478 PLSLEELSEKAGAGDQIETIYKILRH 503

 Score = 25.0 bits (53), Expect(2) = 0.45
 Identities = 11/27 (40%), Positives = 16/27 (58%)
 Frame = -1

Query: 703 KLLLTLAANDRALIAEGSCGSPRSIKV 623
           K+L  LAAN R ++  G+ G P  + V
Sbjct: 499 KILRHLAANKRGIVFHGNVGQPDKLGV 525

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 642,874,816
Number of Sequences: 1393205
Number of extensions: 13112799
Number of successful extensions: 32262
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 30330
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32149
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 42016716300
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR014c12_f BP077023 1 380 2 MR017h04_f BP077310 135 502 3 MR041e07_f BP079175 342 723 4 SPDL079f04_f BP056925 414 817

---