>KMC004188A_C01 KMC004188A_c01
ggcccccttACAGTACAACAACAACACTGCAACTAGAAAACTTCAGAAGAAGAGATGGTG
AGTGTAGCTGAGATTCGTAAGGCCCAGAGGGCTGAAGGCCCAGCAACCATCCTGGCTATG
GGCACTGCAAATCCACCAGACTGTGTTGACCAAAGCACTTATCCTGATTTCTACTTCAAA
ATCACCAACAGTGAACACATGACAGAGCTTAAGGAGAAGTTTCAGCGCATGTGTGACAAG
TCTATGATCAAGAAGAGATATATGTACTTGAATGAAGAAATTTTGAAAGAGAACCCCAAC
CTTTGTGCTTATATGGCACCATCTTTGGATGCTAGGCAGGAGCATGGTGGTGGTAGAGGT
ACCAAGGTTAGGGAAAGAAGCTGCAACAAAGGCTATAAAAGAATGGGGCCAGCCAAAATC
TAAGATTACTCACTTAATCTTTTGCACCACAAGTGGTGTAGACATGCCTGGTGCTGATTA
TCAACTCACCAAACTCTTGGGTCTTCGCCCATCTGTGAAAAGGTACATGATGTACCAGCA
AGGGTGCTTTGCAGGTGGCACGGTGCTTCGTTTGGCTAAAGACTTGGCTGAGAACAACGA
AGGTGCACGTGTGCTAGTTGTTTGTTCTGAGATTACTGCGGTTACATTTCGTGGCCCTAA
TGACACtcacctagacagccttgtggggcaagctttgttcggagacggagcagctgcggt
cattgttggttctgatccagtgcctg

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004188A_C01 KMC004188A_c01
         (746 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q9XJ58|CHS1_CITSI Chalcone synthase 1 (Naringenin-chalcone sy...   251  e-112
pir||S12224 naringenin-chalcone synthase (EC 2.3.1.74) 2 - tomato     239  e-106
gb|AAG43360.1|AF144542_1 chalcone synthase [Ionopsidium abulense]     247  e-103
pir||S12223 naringenin-chalcone synthase (EC 2.3.1.74) 1 - tomato     226  e-103
gb|AAO63021.1| chalcone synthase B [Allium cepa]                      244  e-102

>sp|Q9XJ58|CHS1_CITSI Chalcone synthase 1 (Naringenin-chalcone synthase 1)
           gi|5106367|dbj|BAA81663.1| chalcone synthase [Citrus
           sinensis]
          Length = 389

 Score =  251 bits (642), Expect(2) = e-112
 Identities = 123/136 (90%), Positives = 133/136 (97%)
 Frame = +2

Query: 338 RSMVVVEVPRLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSV 517
           + MVVVEVPRLGK AAT+AIKEWGQPKSKITHL+FCTTSGVDMPGADY+LTKLLGLRPSV
Sbjct: 95  QDMVVVEVPRLGK-AATEAIKEWGQPKSKITHLVFCTTSGVDMPGADYRLTKLLGLRPSV 153

Query: 518 KRYMMYQQGCFAGGTVLRLAKDLAENNEGARVLVVCSEITAVTFRGPNDTHLDSLVGQAL 697
           KR MMYQQGCFAGGTVLRLAKDLAENN+GARVLVVCSEITAVTFRGP+DTHLDSLVGQAL
Sbjct: 154 KRLMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSEITAVTFRGPSDTHLDSLVGQAL 213

Query: 698 FGDGAAAVIVGSDPVP 745
           FGDGAAA+I+G+DP+P
Sbjct: 214 FGDGAAAIIIGADPIP 229

 Score =  176 bits (445), Expect(2) = e-112
 Identities = 80/96 (83%), Positives = 92/96 (95%)
 Frame = +1

Query: 55  MVSVAEIRKAQRAEGPATILAMGTANPPDCVDQSTYPDFYFKITNSEHMTELKEKFQRMC 234
           MV+V E+RKAQRA+GPATI+A+GTA PP+CVDQSTYPD+YF+ITNSEHMT+LKEKF+RMC
Sbjct: 1   MVTVDEVRKAQRAQGPATIMAIGTATPPNCVDQSTYPDYYFRITNSEHMTDLKEKFKRMC 60

Query: 235 DKSMIKKRYMYLNEEILKENPNLCAYMAPSLDARQE 342
           DKSMIKKRYMYL EEILKENPN+CAYMAPSLD RQ+
Sbjct: 61  DKSMIKKRYMYLTEEILKENPNVCAYMAPSLDTRQD 96

>pir||S12224 naringenin-chalcone synthase (EC 2.3.1.74) 2 - tomato
          Length = 410

 Score =  239 bits (611), Expect(2) = e-106
 Identities = 117/136 (86%), Positives = 129/136 (94%)
 Frame = +2

Query: 338 RSMVVVEVPRLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSV 517
           + +VVVEVP+LGKEAA K   EWGQPKSKITH++FCTTSGVDMPGADYQLTKLLGLRPSV
Sbjct: 95  QDIVVVEVPKLGKEAAQK---EWGQPKSKITHVVFCTTSGVDMPGADYQLTKLLGLRPSV 151

Query: 518 KRYMMYQQGCFAGGTVLRLAKDLAENNEGARVLVVCSEITAVTFRGPNDTHLDSLVGQAL 697
           KR MMYQQGCFAGGTV+RLAKDLAENN+GARVLVVCSEITAVTFRGP+DTHLDS+VGQAL
Sbjct: 152 KRLMMYQQGCFAGGTVIRLAKDLAENNKGARVLVVCSEITAVTFRGPSDTHLDSMVGQAL 211

Query: 698 FGDGAAAVIVGSDPVP 745
           FGD AAA+I+GSDP+P
Sbjct: 212 FGDRAAAMIIGSDPLP 227

 Score =  169 bits (427), Expect(2) = e-106
 Identities = 77/96 (80%), Positives = 90/96 (93%)
 Frame = +1

Query: 55  MVSVAEIRKAQRAEGPATILAMGTANPPDCVDQSTYPDFYFKITNSEHMTELKEKFQRMC 234
           MV+V E+R+AQRA+GPATI+A+GTA P +CVDQSTYPD+YF+ITNSEHMTELKEKF+RMC
Sbjct: 1   MVTVEEVRRAQRAKGPATIMAIGTATPSNCVDQSTYPDYYFRITNSEHMTELKEKFKRMC 60

Query: 235 DKSMIKKRYMYLNEEILKENPNLCAYMAPSLDARQE 342
           DKSMI KRYM+L EEILKENPN+C YMAPSLDARQ+
Sbjct: 61  DKSMINKRYMHLTEEILKENPNICEYMAPSLDARQD 96

>gb|AAG43360.1|AF144542_1 chalcone synthase [Ionopsidium abulense]
          Length = 399

 Score =  247 bits (630), Expect(2) = e-103
 Identities = 120/134 (89%), Positives = 129/134 (95%)
 Frame = +2

Query: 338 RSMVVVEVPRLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSV 517
           + +VVVEVP+LGKEAA +AIKEWGQPKSKITH++FCTTSGVDMPGADYQLTKLLGLRPSV
Sbjct: 104 QDIVVVEVPKLGKEAAVQAIKEWGQPKSKITHVVFCTTSGVDMPGADYQLTKLLGLRPSV 163

Query: 518 KRYMMYQQGCFAGGTVLRLAKDLAENNEGARVLVVCSEITAVTFRGPNDTHLDSLVGQAL 697
           KR MMYQQGC+AGGTVLRLAKDLAENN GARVLVVCSEITAVTFRGP+DTHLDSLVGQAL
Sbjct: 164 KRLMMYQQGCYAGGTVLRLAKDLAENNRGARVLVVCSEITAVTFRGPSDTHLDSLVGQAL 223

Query: 698 FGDGAAAVIVGSDP 739
           F DGAAA+IVGSDP
Sbjct: 224 FSDGAAALIVGSDP 237

 Score =  152 bits (383), Expect(2) = e-103
 Identities = 70/95 (73%), Positives = 87/95 (90%)
 Frame = +1

Query: 58  VSVAEIRKAQRAEGPATILAMGTANPPDCVDQSTYPDFYFKITNSEHMTELKEKFQRMCD 237
           +S+ EIRKAQRA+GPA+ILA+GTANP + V Q+ YPD+YF+ITNSEHMT+LKEKF+RMCD
Sbjct: 11  LSLDEIRKAQRADGPASILAIGTANPANHVIQAEYPDYYFRITNSEHMTDLKEKFKRMCD 70

Query: 238 KSMIKKRYMYLNEEILKENPNLCAYMAPSLDARQE 342
           KSMI+KR+M+L EE LKENP++CAYMAPSLD RQ+
Sbjct: 71  KSMIRKRHMHLTEEFLKENPDMCAYMAPSLDVRQD 105

>pir||S12223 naringenin-chalcone synthase (EC 2.3.1.74) 1 - tomato
          Length = 381

 Score =  226 bits (576), Expect(2) = e-103
 Identities = 110/135 (81%), Positives = 122/135 (89%)
 Frame = +2

Query: 338 RSMVVVEVPRLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSV 517
           + +VVVEVP+LGK    K   EWGQPKSKITHL+FCTTSGVDMP  DYQL KLL +RPSV
Sbjct: 95  QDIVVVEVPKLGKRGTQK---EWGQPKSKITHLVFCTTSGVDMPACDYQLAKLLPVRPSV 151

Query: 518 KRYMMYQQGCFAGGTVLRLAKDLAENNEGARVLVVCSEITAVTFRGPNDTHLDSLVGQAL 697
           KR MMYQQGCFAGGTVLRLAKDLAENN+GARVLVVCSEITAVTFRGP+++HLDSLVGQAL
Sbjct: 152 KRLMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSEITAVTFRGPSESHLDSLVGQAL 211

Query: 698 FGDGAAAVIVGSDPV 742
           FGDGAAA+I+GSDP+
Sbjct: 212 FGDGAAAIIIGSDPI 226

 Score =  171 bits (433), Expect(2) = e-103
 Identities = 80/96 (83%), Positives = 90/96 (93%)
 Frame = +1

Query: 55  MVSVAEIRKAQRAEGPATILAMGTANPPDCVDQSTYPDFYFKITNSEHMTELKEKFQRMC 234
           MV+V E RKAQRAEGPATILA+GT+ P +CVDQSTYPD+YF+ITNSEH TELKEKF+RMC
Sbjct: 1   MVTVEEYRKAQRAEGPATILAIGTSTPSNCVDQSTYPDYYFRITNSEHKTELKEKFKRMC 60

Query: 235 DKSMIKKRYMYLNEEILKENPNLCAYMAPSLDARQE 342
           DKSMIKKRYM+L EEILKENPN+CAYMAPSLDARQ+
Sbjct: 61  DKSMIKKRYMHLTEEILKENPNMCAYMAPSLDARQD 96

>gb|AAO63021.1| chalcone synthase B [Allium cepa]
          Length = 393

 Score =  244 bits (624), Expect(2) = e-102
 Identities = 118/134 (88%), Positives = 128/134 (95%)
 Frame = +2

Query: 338 RSMVVVEVPRLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSV 517
           + +VVVEVP+LGK AA  AIKEWG+PK+ ITHLIFCTTSGVDMPGADYQLTKLLGLRPSV
Sbjct: 97  QDIVVVEVPKLGKSAAQSAIKEWGRPKAHITHLIFCTTSGVDMPGADYQLTKLLGLRPSV 156

Query: 518 KRYMMYQQGCFAGGTVLRLAKDLAENNEGARVLVVCSEITAVTFRGPNDTHLDSLVGQAL 697
           KR+MMYQQGCFAGGTVLRLAKDLAENN GARVLVVCSEITAVTFRGP+DTHLDS+VGQAL
Sbjct: 157 KRFMMYQQGCFAGGTVLRLAKDLAENNRGARVLVVCSEITAVTFRGPSDTHLDSMVGQAL 216

Query: 698 FGDGAAAVIVGSDP 739
           FGDGAAA++VGSDP
Sbjct: 217 FGDGAAALVVGSDP 230

 Score =  150 bits (380), Expect(2) = e-102
 Identities = 68/95 (71%), Positives = 84/95 (87%)
 Frame = +1

Query: 58  VSVAEIRKAQRAEGPATILAMGTANPPDCVDQSTYPDFYFKITNSEHMTELKEKFQRMCD 237
           +++ EIR  QRA+GPAT+LA+GTA P + V Q  YPD+YF+ITNSEHMT LKEKF+RMCD
Sbjct: 4   INIDEIRSTQRADGPATVLAIGTATPKNMVYQDEYPDYYFRITNSEHMTGLKEKFKRMCD 63

Query: 238 KSMIKKRYMYLNEEILKENPNLCAYMAPSLDARQE 342
           KSMI+KRYM+L+E+IL ENPN+CAYMAPSLDARQ+
Sbjct: 64  KSMIRKRYMHLSEDILNENPNMCAYMAPSLDARQD 98

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 675,305,251
Number of Sequences: 1393205
Number of extensions: 15075076
Number of successful extensions: 41211
Number of sequences better than 10.0: 591
Number of HSP's better than 10.0 without gapping: 39229
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 41109
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 36032594816
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD021g10_f AV771470 1 537 2 MPD013g06_f AV770909 11 539 3 MPD006f06_f AV770415 57 551 4 MPD021d06_f AV771447 115 619 5 MFB035a09_f BP036542 561 749

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