>KMC004164A_C01 KMC004164A_c01
gacaaaacaaaacctacactaaaaggtgccttatatcattatatcttacaacataagctg
atcagaagtgcacagccctAACTAAATAAGTTATTGTTCGACATTCATTCCATGAAAATT
TGGCAAAATCTAGAAAACTACAATCTGATACAGTTGAAGAAAATCCAACCATAAGTCTAC
TTATCTAGAAAAACAGGAAATCAAAACCCCCTCATAGAATGACTTCTACAATTATAATTT
TGAGGACGGTTATCAAAAACTATACAGAGATACTCAGCTGAGAATCTTCGTTTTGCTGCC
AAAACTGCCATTCCCACCTTCAATGAGACAAAATCTGCGAATAAATTTATTACCAATTAC
AACAAAACCTTGAGCCAAATACTCAGGCTCAGCTGACCATCACCAATTACAACAAAACCT
TGAGCCAAATACTCAGGCTCAGCTGACCATCACCGCAAGATATTCACGAATGCCATCAAT
GAATCAACATCTCTCTTTTCCGAAGGATACTTAATTGGTCGGGAAGAGTGTTTGGGG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004164A_C01 KMC004164A_c01
         (537 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T10065 phosphoadenylyl-sulfate reductase (thioredoxin) (EC ...    57  1e-07
gb|AAB05871.2| PAPS-reductase-like protein [Catharanthus roseus]       57  1e-07
gb|AAL66290.1|AF452450_1 adenosine 5'-phosphosulfate reductase [...    54  9e-07
emb|CAB65911.1| adenosine 5'-phosphosulphate reductase [Lemna mi...    53  3e-06
ref|NP_193930.1| PRH26 protein; protein id: At4g21990.1, support...    51  1e-05

>pir||T10065 phosphoadenylyl-sulfate reductase (thioredoxin) (EC 1.8.4.8)
           precursor, chloroplast - Madagascar periwinkle
          Length = 463

 Score = 57.0 bits (136), Expect = 1e-07
 Identities = 26/28 (92%), Positives = 27/28 (95%)
 Frame = -2

Query: 536 PKHSSRPIKYPSEKRDVDSLMAFVNILR 453
           PKHSS+PIKYPSEKRDVDSLMAFVN LR
Sbjct: 436 PKHSSKPIKYPSEKRDVDSLMAFVNALR 463

>gb|AAB05871.2| PAPS-reductase-like protein [Catharanthus roseus]
          Length = 464

 Score = 57.0 bits (136), Expect = 1e-07
 Identities = 26/28 (92%), Positives = 27/28 (95%)
 Frame = -2

Query: 536 PKHSSRPIKYPSEKRDVDSLMAFVNILR 453
           PKHSS+PIKYPSEKRDVDSLMAFVN LR
Sbjct: 437 PKHSSKPIKYPSEKRDVDSLMAFVNALR 464

>gb|AAL66290.1|AF452450_1 adenosine 5'-phosphosulfate reductase [Glycine max]
          Length = 470

 Score = 54.3 bits (129), Expect = 9e-07
 Identities = 25/28 (89%), Positives = 26/28 (92%)
 Frame = -2

Query: 536 PKHSSRPIKYPSEKRDVDSLMAFVNILR 453
           PKHSS+PIKYPSEKRDVDSL AFVN LR
Sbjct: 443 PKHSSQPIKYPSEKRDVDSLTAFVNALR 470

>emb|CAB65911.1| adenosine 5'-phosphosulphate reductase [Lemna minor]
          Length = 459

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 22/28 (78%), Positives = 27/28 (95%)
 Frame = -2

Query: 536 PKHSSRPIKYPSEKRDVDSLMAFVNILR 453
           PKH+S+P+KYPSE+RDVDSL+AFVN LR
Sbjct: 432 PKHASKPVKYPSEQRDVDSLLAFVNALR 459

>ref|NP_193930.1| PRH26 protein; protein id: At4g21990.1, supported by cDNA: 36866.,
           supported by cDNA: gi_15809815, supported by cDNA:
           gi_16226613, supported by cDNA: gi_17065177, supported
           by cDNA: gi_20259941 [Arabidopsis thaliana]
           gi|24211446|sp|P92980|APR3_ARATH 5'-adenylylsulfate
           reductase 3, chloroplast precursor (Adenosine
           5'-phosphosulfate 5'-adenylylsulfate sulfotransferase 3)
           (APS sulfotransferase 3) (Thioredoxin independent APS
           reductase 3) (3'-phosphoadenosine-5'-phosphosulfate
           reductase homolog 26) (PAPS reductase homolog 26)
           (Prh-26) gi|11358631|pir||T49106 PRH26 protein -
           Arabidopsis thaliana gi|2738760|gb|AAC26981.1|
           5'-adenylylsulfate reductase [Arabidopsis thaliana]
           gi|2961344|emb|CAA18102.1| PRH26 protein [Arabidopsis
           thaliana] gi|7269044|emb|CAB79154.1| PRH26 protein
           [Arabidopsis thaliana] gi|15809816|gb|AAL06836.1|
           AT4g21990/F1N20_90 [Arabidopsis thaliana]
           gi|16226614|gb|AAL16214.1|AF428445_1 AT4g21990/F1N20_90
           [Arabidopsis thaliana] gi|17065178|gb|AAL32743.1| PRH26
           protein [Arabidopsis thaliana]
           gi|20259942|gb|AAM13318.1| PRH26 protein [Arabidopsis
           thaliana] gi|21655305|gb|AAM65364.1| AT4g21990/F1N20_90
           [Arabidopsis thaliana]
          Length = 458

 Score = 50.8 bits (120), Expect = 1e-05
 Identities = 22/28 (78%), Positives = 27/28 (95%)
 Frame = -2

Query: 536 PKHSSRPIKYPSEKRDVDSLMAFVNILR 453
           PK+SSRPIKYPSEKRDVDSL +F+N++R
Sbjct: 431 PKNSSRPIKYPSEKRDVDSLTSFLNLVR 458

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 429,978,662
Number of Sequences: 1393205
Number of extensions: 8343750
Number of successful extensions: 17223
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 16444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17207
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18173652336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWL059h04_f AV769612 1 537 2 MR009h06_f BP076664 80 460

---