Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004151A_C01 KMC004151A_c01
(698 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAB41036.1| GA 2-oxidase [Phaseolus coccineus] 140 2e-34
ref|NP_174296.1| unknown protein; protein id: At1g30040.1 [Arabi... 130 4e-30
emb|CAC83090.1| gibberellin 2-oxidase [Cucurbita maxima] 131 1e-29
emb|CAA70330.1| dioxygenase [Marah macrocarpus] 130 1e-29
dbj|BAB12442.1| gibberellin 2-oxidase No1 [Lactuca sativa] 124 6e-29
>emb|CAB41036.1| GA 2-oxidase [Phaseolus coccineus]
Length = 332
Score = 140 bits (354), Expect(2) = 2e-34
Identities = 68/80 (85%), Positives = 72/80 (90%)
Frame = -1
Query: 698 PDHTSFFINVGDTLQVMTNGRFKSVKHRVMADTTKSRLSMIYFGGASLSEKIAPLESLMS 519
PD TSFFINVGD LQVMTNGRFKSVKHRV+ADTTKSRLSMIYFGG +LSE IAPL S+M
Sbjct: 235 PDQTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVML 294
Query: 518 KGEQSLYKEFTWSEYKKAAY 459
KGE+ LYKEFTW EYKKAAY
Sbjct: 295 KGEECLYKEFTWCEYKKAAY 314
Score = 26.9 bits (58), Expect(2) = 2e-34
Identities = 11/19 (57%), Positives = 14/19 (72%)
Frame = -2
Query: 472 RKLRTSSRLGDNRLGPFEK 416
+K +SRL DNRL PF+K
Sbjct: 310 KKAAYTSRLADNRLAPFQK 328
>ref|NP_174296.1| unknown protein; protein id: At1g30040.1 [Arabidopsis thaliana]
gi|25285702|pir||T52578 gibberellin 2beta-dioxygenase
(EC 1.14.11.13) 2 [validated] - Arabidopsis thaliana
gi|4678368|emb|CAB41008.1| GA 2-oxidase [Arabidopsis
thaliana] gi|12324163|gb|AAG52050.1|AC022455_4 unknown
protein; 59645-61446 [Arabidopsis thaliana]
gi|27754705|gb|AAO22796.1| unknown protein [Arabidopsis
thaliana] gi|28394101|gb|AAO42458.1| unknown protein
[Arabidopsis thaliana]
Length = 341
Score = 130 bits (326), Expect(2) = 4e-30
Identities = 61/85 (71%), Positives = 72/85 (83%)
Frame = -1
Query: 698 PDHTSFFINVGDTLQVMTNGRFKSVKHRVMADTTKSRLSMIYFGGASLSEKIAPLESLMS 519
PDH+SFFINVGD LQVMTNGRFKSVKHRV+ADT +SR+SMIYFGG LS+KIAPL L+
Sbjct: 238 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 297
Query: 518 KGEQSLYKEFTWSEYKKAAYQFKTG 444
+ + LYKEFTWS+YK +AY+ K G
Sbjct: 298 EQDDWLYKEFTWSQYKSSAYKSKLG 322
Score = 23.5 bits (49), Expect(2) = 4e-30
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = -2
Query: 454 SRLGDNRLGPFEKK 413
S+LGD RLG FEK+
Sbjct: 319 SKLGDYRLGLFEKQ 332
>emb|CAC83090.1| gibberellin 2-oxidase [Cucurbita maxima]
Length = 321
Score = 131 bits (329), Expect = 1e-29
Identities = 62/80 (77%), Positives = 72/80 (89%)
Frame = -1
Query: 698 PDHTSFFINVGDTLQVMTNGRFKSVKHRVMADTTKSRLSMIYFGGASLSEKIAPLESLMS 519
PDH+SFFINVGD+LQVMTNGRFKSVKHRV+ +++KSR+SMIYFGG LSEKIAPL SLM
Sbjct: 224 PDHSSFFINVGDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQ 283
Query: 518 KGEQSLYKEFTWSEYKKAAY 459
E+SLYKEFTW EYK++AY
Sbjct: 284 GEERSLYKEFTWFEYKRSAY 303
>emb|CAA70330.1| dioxygenase [Marah macrocarpus]
Length = 322
Score = 130 bits (328), Expect(2) = 1e-29
Identities = 64/80 (80%), Positives = 72/80 (90%)
Frame = -1
Query: 698 PDHTSFFINVGDTLQVMTNGRFKSVKHRVMADTTKSRLSMIYFGGASLSEKIAPLESLMS 519
PD TSFFINVGD+LQVMTNGRFKSVKHRV+ ++ KSR+SMIYFGG LSEKIAPL SLM
Sbjct: 226 PDQTSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLM- 284
Query: 518 KGEQSLYKEFTWSEYKKAAY 459
KGE+SLYKEFTW EYK++AY
Sbjct: 285 KGEESLYKEFTWFEYKRSAY 304
Score = 21.2 bits (43), Expect(2) = 1e-29
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = -2
Query: 457 SSRLGDNRLGPFEK 416
+SRL DNRL FE+
Sbjct: 305 NSRLADNRLVHFER 318
>dbj|BAB12442.1| gibberellin 2-oxidase No1 [Lactuca sativa]
Length = 337
Score = 124 bits (312), Expect(2) = 6e-29
Identities = 61/79 (77%), Positives = 69/79 (87%)
Frame = -1
Query: 695 DHTSFFINVGDTLQVMTNGRFKSVKHRVMADTTKSRLSMIYFGGASLSEKIAPLESLMSK 516
D SFFINVGD+LQVMTNGRFKSVKHRV+A++TKSR+SMIYFGG LSEKIAPL SL+
Sbjct: 241 DSDSFFINVGDSLQVMTNGRFKSVKHRVVANSTKSRVSMIYFGGPPLSEKIAPLPSLIQG 300
Query: 515 GEQSLYKEFTWSEYKKAAY 459
E SLYKEFTW EYKK+A+
Sbjct: 301 EEDSLYKEFTWFEYKKSAF 319
Score = 25.0 bits (53), Expect(2) = 6e-29
Identities = 11/19 (57%), Positives = 14/19 (72%)
Frame = -2
Query: 472 RKLRTSSRLGDNRLGPFEK 416
+K ++RL DNRLG FEK
Sbjct: 315 KKSAFNTRLADNRLGLFEK 333
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 545,432,279
Number of Sequences: 1393205
Number of extensions: 10926163
Number of successful extensions: 37392
Number of sequences better than 10.0: 564
Number of HSP's better than 10.0 without gapping: 31287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35561
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31967457276
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
