>KMC004113A_C01 KMC004113A_c01
ggtaaaaaaacaagaaaaacagatttgaataaagaacttggtgatcttcgctaggcatct
agctaacagtaataattttcAAGGATTTTCTCAAAGATAAAAAAATAAAATAAAATTCCC
TAGCTGTACACAGAATCAAAACGTTCATCAAGTTCTCCAATTCAAGCCCTTTACCTTTTC
TTTATTCTCTCTATTTTCCTTTTTTCTTTTTTTCCAACCTCGCACCTCAAACGACATCGT
TTTGCACAACTACCTCATTTTCCACGCCCGCACCACCGTCACCGCCACCGTCCTCAACCC
ACTCTTCCACCGGCGCACACTCCATCTTGTGCCGCAACTTCCAATCCAGCGCCTGACACC
CCCGCGAGCAGTAATTCACCTTCCCGCAAACCGAGCACCGCCGGAACTCATGCGGCCGCG
TCTCCGGCCGGCCACACCCAATGTGACCGCACATCCTCAGCCCCTCACCTAGCTCTCCCC
GCCCCGACGCAAACCACTCCCTGAGAAACCAATTCGCCGGATGCACCTCCGGCACCGCCA
CGCCGTAGTCACTCATCAACGGCGCCCTCGTCACGGCGCGTAAAACCAACGCAAGCTCCC
GCAGGTTCGCCTGCACGAGCAAACGCCGCCCCTCCGCAACGTTCATCTTCACGCCGTAAC
CGTCCTGGAGACAATGCCCAAGCTCCCGGAGCGCGTCGACGTGACCTAGCAACGACGCCC
GCGCCGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004113A_C01 KMC004113A_c01
         (727 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_564894.1| F-box protein family; protein id: At1g67340.1, ...   221  6e-57
ref|NP_199856.1| putative protein; protein id: At5g50450.1, supp...   215  6e-55
dbj|BAB86182.1| OJ1485_B09.11 [Oryza sativa (japonica cultivar-g...   179  5e-44
pir||A60533 tumor-associated antigen DF3 - human                       69  9e-11
ref|NP_789547.1| proline/alanine-rich repetetive membrane anchor...    65  1e-09

>ref|NP_564894.1| F-box protein family; protein id: At1g67340.1, supported by cDNA:
           gi_16209725, supported by cDNA: gi_20260405 [Arabidopsis
           thaliana] gi|25373198|pir||H96696 protein F1N21.16
           [imported] - Arabidopsis thaliana
           gi|9828618|gb|AAG00241.1|AC002130_6 F1N21.16
           [Arabidopsis thaliana] gi|16209726|gb|AAL14418.1|
           At1g67340/F1N21_16 [Arabidopsis thaliana]
           gi|20260406|gb|AAM13101.1| unknown protein [Arabidopsis
           thaliana] gi|23197876|gb|AAN15465.1| unknown protein
           [Arabidopsis thaliana]
          Length = 379

 Score =  221 bits (564), Expect = 6e-57
 Identities = 111/182 (60%), Positives = 128/182 (69%), Gaps = 27/182 (14%)
 Frame = -1

Query: 724 ARASLLGHVDALRELGHCLQDGYGVKMNVAEGRRLLVQANLRELALVLRAVTRA------ 563
           ARA+ LGHVDALRELGHCLQDGYGV  NV+EGRR LVQAN RELA VL +  +A      
Sbjct: 185 ARAAFLGHVDALRELGHCLQDGYGVPQNVSEGRRFLVQANARELAAVLSSGIQARSTWLS 244

Query: 562 -----------------PLMSDYG--VAVPEVHPANWFLREWFASGRGEL-GEGLRMCGH 443
                            PL+SD+G  V  PE HPAN FL +WFA   G+  G+GLR+C H
Sbjct: 245 LSQPPPPVVPNHGQQTCPLLSDFGCNVPAPETHPANRFLADWFAVRGGDCPGDGLRLCSH 304

Query: 442 IGCGRPETRPHEFRRCSVCGKVNYCSRGCQALDWKLRHKMECAPVEEWVEDG-GGDGGAG 266
            GCGRPETR HEFRRCSVCG VNYCSR CQALDWKLRHKM+CAPV+ W+E+G GG+G   
Sbjct: 305 AGCGRPETRKHEFRRCSVCGVVNYCSRACQALDWKLRHKMDCAPVQRWLEEGDGGEGNVQ 364

Query: 265 VE 260
           ++
Sbjct: 365 ID 366

>ref|NP_199856.1| putative protein; protein id: At5g50450.1, supported by cDNA:
           gi_20466553 [Arabidopsis thaliana]
           gi|9758927|dbj|BAB09464.1|
           gb|AAB95234.1~gene_id:MXI22.17~strong similarity to
           unknown protein [Arabidopsis thaliana]
           gi|20466554|gb|AAM20594.1| putative protein [Arabidopsis
           thaliana] gi|23198134|gb|AAN15594.1| putative protein
           [Arabidopsis thaliana]
          Length = 336

 Score =  215 bits (547), Expect = 6e-55
 Identities = 105/167 (62%), Positives = 123/167 (72%), Gaps = 9/167 (5%)
 Frame = -1

Query: 724 ARASLLGHVDALRELGHCLQDGYGVKMNVAEGRRLLVQANLRELALVLRAVTRAP----- 560
           AR++ LGHVDALRELGHCLQDGYGV  +V+EGRRLL+QAN RELA  LR+          
Sbjct: 163 ARSAYLGHVDALRELGHCLQDGYGVPRDVSEGRRLLIQANARELACSLRSYLSLKSGDEN 222

Query: 559 -LMSDYGVA-VPEVHPANWFLREWFASGRGELGEGLRMCGHIGCGRPETRPHEFRRCSVC 386
             ++D  V  V E+HP N FL+EWF+SGR +L EGLRMC H GCGRPETR HEFRRCSVC
Sbjct: 223 ETLTDLSVVPVQEIHPVNRFLKEWFSSGRVDLAEGLRMCSHGGCGRPETRAHEFRRCSVC 282

Query: 385 GKVNYCSRGCQALDWKLRHKMECAPVEEWVEDGG--GDGGAGVENEV 251
           GKVNYCSRGCQALDW+ +HK+EC P++ WV      GD G  V  E+
Sbjct: 283 GKVNYCSRGCQALDWRAKHKVECTPLDLWVAAAAEIGDDGEAVAVEI 329

>dbj|BAB86182.1| OJ1485_B09.11 [Oryza sativa (japonica cultivar-group)]
          Length = 388

 Score =  179 bits (453), Expect = 5e-44
 Identities = 93/187 (49%), Positives = 110/187 (58%), Gaps = 40/187 (21%)
 Frame = -1

Query: 724 ARASLLGHVDALRELGHCLQDGYGVKMNVAEGRRLLVQANLRELALVLRAVTR------- 566
           ARA+ LGHVDALRELGHCLQDGYGV+ + AEGRR LV AN REL L L A          
Sbjct: 199 ARAAALGHVDALRELGHCLQDGYGVRRDPAEGRRFLVAANARELTLALAAAASHRPFAAL 258

Query: 565 ------------APLMSDYGVAVPEV--HPANWFLREWFAS------------------- 485
                        PL+SD+G ++PE   HPAN F+ +W+AS                   
Sbjct: 259 PLAGGAAAGAIGCPLLSDFGWSLPEAEPHPANLFMADWWASRGVQATAKKPGLEAPAAAT 318

Query: 484 GRGELGEGLRMCGHIGCGRPETRPHEFRRCSVCGKVNYCSRGCQALDWKLRHKMECAPVE 305
           G  + G  LR+C H+ CGR ETR HEFRRCSVCG  NYCSR CQALDWK  HK +C P++
Sbjct: 319 GDSDGGGELRLCSHVRCGRRETRRHEFRRCSVCGAANYCSRACQALDWKRAHKAQCVPMD 378

Query: 304 EWVEDGG 284
            W+   G
Sbjct: 379 RWLLAAG 385

>pir||A60533 tumor-associated antigen DF3 - human
          Length = 256

 Score = 68.6 bits (166), Expect = 9e-11
 Identities = 57/160 (35%), Positives = 78/160 (48%), Gaps = 12/160 (7%)
 Frame = +1

Query: 283 RHRPQPTLPPAHTPSCAATSNPAPDTPAS-----SNSPSRKPSTAGTHAAASPAGHTQCD 447
           R  P  T PPAH  + A  S PAP + A      +++P  +P+   T    +P  H    
Sbjct: 8   RPAPGSTAPPAHRVTSAPESRPAPGSTAPPAHRVTSAPESRPAPGST----APPAH---- 59

Query: 448 RTSSAPHLALPAPTQTTP*E---TNSPDAPPAPPRRSHSSTAPSSRRVKPTQAPAGSPAR 618
           R +SAP  + PAP  T P     T++P++ PAP      STAP + RV  T AP   PA 
Sbjct: 60  RVTSAPE-SRPAPGSTAPPAHRVTSAPESRPAP-----GSTAPPAHRV--TSAPESRPAP 111

Query: 619 ANAAPPQR--SSSRRNRPGDNAQAPGARRRDLA--TTPAP 726
            + APP    +S+  +RP   + AP A R   A  + PAP
Sbjct: 112 GSTAPPAHRVTSAPESRPAPGSTAPPAHRVTSAPESRPAP 151

 Score = 68.6 bits (166), Expect = 9e-11
 Identities = 57/160 (35%), Positives = 78/160 (48%), Gaps = 12/160 (7%)
 Frame = +1

Query: 283 RHRPQPTLPPAHTPSCAATSNPAPDTPAS-----SNSPSRKPSTAGTHAAASPAGHTQCD 447
           R  P  T PPAH  + A  S PAP + A      +++P  +P+   T    +P  H    
Sbjct: 48  RPAPGSTAPPAHRVTSAPESRPAPGSTAPPAHRVTSAPESRPAPGST----APPAH---- 99

Query: 448 RTSSAPHLALPAPTQTTP*E---TNSPDAPPAPPRRSHSSTAPSSRRVKPTQAPAGSPAR 618
           R +SAP  + PAP  T P     T++P++ PAP      STAP + RV  T AP   PA 
Sbjct: 100 RVTSAPE-SRPAPGSTAPPAHRVTSAPESRPAP-----GSTAPPAHRV--TSAPESRPAP 151

Query: 619 ANAAPPQR--SSSRRNRPGDNAQAPGARRRDLA--TTPAP 726
            + APP    +S+  +RP   + AP A R   A  + PAP
Sbjct: 152 GSTAPPAHRVTSAPESRPAPGSTAPPAHRVTSAPESRPAP 191

 Score = 64.7 bits (156), Expect = 1e-09
 Identities = 51/145 (35%), Positives = 71/145 (48%), Gaps = 10/145 (6%)
 Frame = +1

Query: 283 RHRPQPTLPPAHTPSCAATSNPAPDTPAS-----SNSPSRKPSTAGTHAAASPAGHTQCD 447
           R  P  T PPAH  + A  S PAP + A      +++P  +P+   T    +P  H    
Sbjct: 68  RPAPGSTAPPAHRVTSAPESRPAPGSTAPPAHRVTSAPESRPAPGST----APPAH---- 119

Query: 448 RTSSAPHLALPAPTQTTP*E---TNSPDAPPAPPRRSHSSTAPSSRRVKPTQAPAGSPAR 618
           R +SAP  + PAP  T P     T++P++ PAP      STAP + RV  T AP   PA 
Sbjct: 120 RVTSAPE-SRPAPGSTAPPAHRVTSAPESRPAP-----GSTAPPAHRV--TSAPESRPAP 171

Query: 619 ANAAPPQR--SSSRRNRPGDNAQAP 687
            + APP    +S+  +RP   + AP
Sbjct: 172 GSTAPPAHRVTSAPESRPAPGSTAP 196

 Score = 42.4 bits (98), Expect = 0.007
 Identities = 38/124 (30%), Positives = 52/124 (41%), Gaps = 11/124 (8%)
 Frame = +1

Query: 283 RHRPQPTLPPAHTPSCAATSNPAPDTPAS-----SNSPSRKPSTAGTHAAASPAGHTQCD 447
           R  P  T PPAH  + A  S PAP + A      +++P  +P+   T    +P  H    
Sbjct: 128 RPAPGSTAPPAHRVTSAPESRPAPGSTAPPAHRVTSAPESRPAPGST----APPAH---- 179

Query: 448 RTSSAPHLALPAPTQTTP*ETNSPDAPPAPPRRSHSSTA------PSSRRVKPTQAPAGS 609
           R +SAP  + PAP  T P        P   P+  H +         +S R   T+ P   
Sbjct: 180 RVTSAPE-SRPAPGSTAP--------PVHNPQALHQAQLLLCAHNGTSARATTTRLPQPQ 230

Query: 610 PARA 621
           PARA
Sbjct: 231 PARA 234

>ref|NP_789547.1| proline/alanine-rich repetetive membrane anchored protein
           [Tropheryma whipplei TW08/27]
           gi|28410900|emb|CAD67285.1| proline/alanine-rich
           repetetive membrane anchored protein [Tropheryma
           whipplei TW08/27]
          Length = 322

 Score = 64.7 bits (156), Expect = 1e-09
 Identities = 55/153 (35%), Positives = 74/153 (47%), Gaps = 10/153 (6%)
 Frame = +1

Query: 295 QPTLPPAHTPSCAATSNPAPDTPASSNS-----PSRKPSTAGTHAAASPAGHTQCDRTSS 459
           QPT+P A  P  AA S PAP  PA S +     P  KP+ A TH++++ A  +     ++
Sbjct: 18  QPTVPVAPAP--AAPSAPAPAKPAPSEATQAAQPPAKPAAA-THSSSTQAPSSAPKPAAA 74

Query: 460 APHLALPAPTQTTP*ETNSPDAPPAPPRRSHSSTAPSS----RRVKPTQA-PAGSPARAN 624
            P  A PAP++ T  +   P A PA    S S+ APSS       KP  A PA S A   
Sbjct: 75  KPAPAKPAPSEAT--QAAQPPAKPAAATHSSSTQAPSSAPKPAAAKPAPAKPAPSEATQA 132

Query: 625 AAPPQRSSSRRNRPGDNAQAPGARRRDLATTPA 723
           A PP + ++ +  P      P A +   AT PA
Sbjct: 133 AQPPAKPAAAKPAPA----KPAATQATQATKPA 161

 Score = 51.6 bits (122), Expect = 1e-05
 Identities = 51/176 (28%), Positives = 71/176 (39%), Gaps = 16/176 (9%)
 Frame = +1

Query: 244 AQLPHFPRPHHRHRHRPQPTLPPAHT-------PSCA---ATSNPAPDTPASSNS----- 378
           A  P  P P    +    P  P A T       PS A   A + PAP  PA S +     
Sbjct: 32  APAPAKPAPSEATQAAQPPAKPAAATHSSSTQAPSSAPKPAAAKPAPAKPAPSEATQAAQ 91

Query: 379 PSRKPSTAGTHAAASPAGHTQCDRTSSAPHLALPAPTQTTP*ETNSPDAPPAPPRRSHSS 558
           P  KP      AAA+ +  TQ   ++  P  A PAP +  P E      PPA P  +  +
Sbjct: 92  PPAKP------AAATHSSSTQAPSSAPKPAAAKPAPAKPAPSEATQAAQPPAKPAAAKPA 145

Query: 559 TA-PSSRRVKPTQAPAGSPARANAAPPQRSSSRRNRPGDNAQAPGARRRDLATTPA 723
            A P++ +      PA +PA+  AA P  ++        + QAP    +  A  P+
Sbjct: 146 PAKPAATQATQATKPA-APAKPAAAKPAAATH-----SSSTQAPSQVTKPAAEKPS 195

 Score = 42.7 bits (99), Expect = 0.005
 Identities = 44/186 (23%), Positives = 69/186 (36%), Gaps = 15/186 (8%)
 Frame = +1

Query: 214 PTSHLKRHRFAQLPHFPRPHHRHRHRPQPTLPPAHTPSCAATSNPAPDTPASSNSPSRKP 393
           P+S  K       P  P P    +    P  P A T S +  +  +   PA++     KP
Sbjct: 65  PSSAPKPAAAKPAPAKPAPSEATQAAQPPAKPAAATHSSSTQAPSSAPKPAAAKPAPAKP 124

Query: 394 STAGTHAAASPAGHTQCDRTSSAPHLALPAPTQTTP*ETNSPDAP-------PAPPRRSH 552
           + +    AA P         ++ P  A PA TQ T  +   P AP       PA    S 
Sbjct: 125 APSEATQAAQPPAKP----AAAKPAPAKPAATQAT--QATKPAAPAKPAAAKPAAATHSS 178

Query: 553 SSTAPSSRRVKPTQAPAGS--------PARANAAPPQRSSSRRNRPGDNAQAPGARRRDL 708
           S+ APS       + P+ +        PA A  AP + ++++  +    AQ       + 
Sbjct: 179 STQAPSQVTKPAAEKPSSTVANTQVTKPAAAKPAPAKPAAAKPTQTTQAAQPSSGNSAER 238

Query: 709 ATTPAP 726
           + +  P
Sbjct: 239 SDSVQP 244

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 706,836,206
Number of Sequences: 1393205
Number of extensions: 19850918
Number of successful extensions: 200322
Number of sequences better than 10.0: 6412
Number of HSP's better than 10.0 without gapping: 102396
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165782
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 34062062287
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR005g02_f BP076339 1 381 2 MWM193f01_f AV767676 214 727

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