Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004025A_C01 KMC004025A_c01
(579 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P54260|GCST_SOLTU Aminomethyltransferase, mitochondrial precu... 310 7e-89
sp|P49363|GCST_FLAPR Aminomethyltransferase, mitochondrial precu... 309 3e-88
ref|NP_172650.1| aminomethyltransferase-like precursor protein; ... 311 6e-88
sp|O23936|GCST_FLATR Aminomethyltransferase, mitochondrial precu... 309 6e-88
sp|P49364|GCST_PEA Aminomethyltransferase, mitochondrial precurs... 309 8e-88
>sp|P54260|GCST_SOLTU Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT) gi|2129976|pir||S59948
aminomethyltransferase (EC 2.1.2.10) precursor - potato
gi|438254|emb|CAA81081.1| T-protein [Solanum tuberosum]
Length = 406
Score = 310 bits (793), Expect(2) = 7e-89
Identities = 147/164 (89%), Positives = 156/164 (94%)
Frame = +2
Query: 86 DKKAVACRYFASEAELKKTALYDFHVVNGGKMVPFAGWSMPIQYKDSIMDSTLNCRENGS 265
DKK + R FAS+A+LKKT LYDFHVVNGGKMVPFAGWSMPIQYKDSIMDST+NCRENGS
Sbjct: 20 DKKTIGRRCFASDADLKKTVLYDFHVVNGGKMVPFAGWSMPIQYKDSIMDSTVNCRENGS 79
Query: 266 LFDVSHMCGLSLKGKDAVPFLEKLVIADVASLAPGTGSLTVFTNEKGGAIDDSVITKVAD 445
LFDVSHMCGLSLKGKD +PFLEKLVIADVA LAPGTGSLTVFTNEKGGAIDDSV+TKV +
Sbjct: 80 LFDVSHMCGLSLKGKDTIPFLEKLVIADVAGLAPGTGSLTVFTNEKGGAIDDSVVTKVTN 139
Query: 446 DHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLL 577
DHIYLVVNAGCRDKDLAHIEEHMK+FK+KGGDVSWHIHDERSLL
Sbjct: 140 DHIYLVVNAGCRDKDLAHIEEHMKSFKSKGGDVSWHIHDERSLL 183
Score = 39.7 bits (91), Expect(2) = 7e-89
Identities = 17/24 (70%), Positives = 20/24 (82%)
Frame = +3
Query: 30 MRGGLWQLGQSITRRLTQVIRRLL 101
MRGGLWQLGQSITRRL Q ++ +
Sbjct: 1 MRGGLWQLGQSITRRLAQADKKTI 24
>sp|P49363|GCST_FLAPR Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT) gi|1362139|pir||S56660
aminomethyltransferase (EC 2.1.2.10) precursor -
Flaveria pringlei gi|438005|emb|CAA81077.1| T protein
[Flaveria pringlei]
Length = 407
Score = 309 bits (791), Expect(2) = 3e-88
Identities = 148/164 (90%), Positives = 155/164 (94%)
Frame = +2
Query: 86 DKKAVACRYFASEAELKKTALYDFHVVNGGKMVPFAGWSMPIQYKDSIMDSTLNCRENGS 265
DKK +A R +ASEA+LKKT LYDFHV NGGKMVPFAGWSMPIQYKDSIM+ST+NCRENGS
Sbjct: 20 DKKTIARRCYASEADLKKTVLYDFHVANGGKMVPFAGWSMPIQYKDSIMESTINCRENGS 79
Query: 266 LFDVSHMCGLSLKGKDAVPFLEKLVIADVASLAPGTGSLTVFTNEKGGAIDDSVITKVAD 445
LFDVSHMCGLSLKGKD VPFLEKLV+ADVA L PGTGSLTVFTNEKGGAIDDSVITKV D
Sbjct: 80 LFDVSHMCGLSLKGKDCVPFLEKLVVADVAGLRPGTGSLTVFTNEKGGAIDDSVITKVTD 139
Query: 446 DHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLL 577
DHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHI+DERSLL
Sbjct: 140 DHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIYDERSLL 183
Score = 38.1 bits (87), Expect(2) = 3e-88
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 30 MRGGLWQLGQSITRRLTQ 83
MRGGLWQLGQSITRRL Q
Sbjct: 1 MRGGLWQLGQSITRRLGQ 18
>ref|NP_172650.1| aminomethyltransferase-like precursor protein; protein id:
At1g11860.1 [Arabidopsis thaliana]
gi|18206365|sp|O65396|GCST_ARATH Aminomethyltransferase,
mitochondrial precursor (Glycine cleavage system T
protein) (GCVT) gi|25328307|pir||H86252 hypothetical
protein [imported] - Arabidopsis thaliana
gi|3157944|gb|AAC17627.1| Very strong similarity to
aminomethyltransferase precursor gb|U79769 from
Mesembryanthemum crystallinum. ESTs gb|T43167,
gb|T21076, gb|H36999, gb|T22773, gb|N38038, gb|T13742,
gb|Z26545, gb|T20753 and gb|W43123 come from this gene.
[Arabidopsis thaliana] gi|21928147|gb|AAM78101.1|
At1g11860/F12F1_30 [Arabidopsis thaliana]
Length = 408
Score = 311 bits (798), Expect(2) = 6e-88
Identities = 149/164 (90%), Positives = 157/164 (94%)
Frame = +2
Query: 86 DKKAVACRYFASEAELKKTALYDFHVVNGGKMVPFAGWSMPIQYKDSIMDSTLNCRENGS 265
DKK V+ RYFASEA+LKKTALYDFHV +GGKMVPFAGWSMPIQYKDSIMDST+NCRENGS
Sbjct: 21 DKKVVSRRYFASEADLKKTALYDFHVAHGGKMVPFAGWSMPIQYKDSIMDSTVNCRENGS 80
Query: 266 LFDVSHMCGLSLKGKDAVPFLEKLVIADVASLAPGTGSLTVFTNEKGGAIDDSVITKVAD 445
LFDV+HMCGLSLKGKD VPFLE LV+ADVA LAPGTGSLTVFTNEKGGAIDDSVITKV D
Sbjct: 81 LFDVAHMCGLSLKGKDCVPFLETLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITKVTD 140
Query: 446 DHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLL 577
+HIYLVVNAGCRDKDLAHIEEHMKAFK+KGGDVSWHIHDERSLL
Sbjct: 141 EHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDERSLL 184
Score = 34.7 bits (78), Expect(2) = 6e-88
Identities = 17/25 (68%), Positives = 21/25 (84%), Gaps = 1/25 (4%)
Frame = +3
Query: 30 MRGG-LWQLGQSITRRLTQVIRRLL 101
MRGG LWQLGQSITRRL Q ++++
Sbjct: 1 MRGGSLWQLGQSITRRLAQSDKKVV 25
>sp|O23936|GCST_FLATR Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT) gi|2467117|emb|CAB16917.1|
T-Protein precursor [Flaveria trinervia]
Length = 407
Score = 309 bits (791), Expect(2) = 6e-88
Identities = 147/164 (89%), Positives = 155/164 (93%)
Frame = +2
Query: 86 DKKAVACRYFASEAELKKTALYDFHVVNGGKMVPFAGWSMPIQYKDSIMDSTLNCRENGS 265
DKK + R++ASEA+LKKT LYDFHV NGGKMVPFAGWSMPIQYKDSIM+ST+NCRENGS
Sbjct: 20 DKKTIVRRWYASEADLKKTVLYDFHVANGGKMVPFAGWSMPIQYKDSIMESTINCRENGS 79
Query: 266 LFDVSHMCGLSLKGKDAVPFLEKLVIADVASLAPGTGSLTVFTNEKGGAIDDSVITKVAD 445
LFDVSHMCGLSLKGKD V FLEKLV+ADVA LAPGTGSLTVFTNEKGGAIDDSVITKV D
Sbjct: 80 LFDVSHMCGLSLKGKDCVAFLEKLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITKVTD 139
Query: 446 DHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLL 577
DHIYLVVNAGCRDKDLAHIE+HMKAFKAKGGDVSWHIHDERSLL
Sbjct: 140 DHIYLVVNAGCRDKDLAHIEQHMKAFKAKGGDVSWHIHDERSLL 183
Score = 37.4 bits (85), Expect(2) = 6e-88
Identities = 16/25 (64%), Positives = 21/25 (84%)
Frame = +3
Query: 30 MRGGLWQLGQSITRRLTQVIRRLLL 104
MRGGLWQ+GQSITRRL Q ++ ++
Sbjct: 1 MRGGLWQVGQSITRRLGQSDKKTIV 25
>sp|P49364|GCST_PEA Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT) gi|1362061|pir||S56661
aminomethyltransferase (EC 2.1.2.10) precursor - garden
pea gi|438217|emb|CAA81080.1| T-protein [Pisum sativum]
gi|3021553|emb|CAA10976.1| T protein [Pisum sativum]
Length = 408
Score = 309 bits (791), Expect(2) = 8e-88
Identities = 150/165 (90%), Positives = 157/165 (94%)
Frame = +2
Query: 83 GDKKAVACRYFASEAELKKTALYDFHVVNGGKMVPFAGWSMPIQYKDSIMDSTLNCRENG 262
GDKKAVA R FA+E+ELKKT LYDFHV +GGKMVPFAGWSMPIQYKDSIMDSTLNCR+NG
Sbjct: 20 GDKKAVARRCFATESELKKTVLYDFHVAHGGKMVPFAGWSMPIQYKDSIMDSTLNCRQNG 79
Query: 263 SLFDVSHMCGLSLKGKDAVPFLEKLVIADVASLAPGTGSLTVFTNEKGGAIDDSVITKVA 442
SLFDVSHMCGLSLKGKD V FLEKLVIADVA+LA GTG+LTVFTNEKGGAIDDSVITKV
Sbjct: 80 SLFDVSHMCGLSLKGKDVVSFLEKLVIADVAALAHGTGTLTVFTNEKGGAIDDSVITKVT 139
Query: 443 DDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLL 577
DDH+YLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLL
Sbjct: 140 DDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLL 184
Score = 37.0 bits (84), Expect(2) = 8e-88
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +3
Query: 30 MRGGLWQLGQSITRRL 77
MRGGLWQLGQSITRRL
Sbjct: 1 MRGGLWQLGQSITRRL 16
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 553,394,193
Number of Sequences: 1393205
Number of extensions: 12607587
Number of successful extensions: 34145
Number of sequences better than 10.0: 234
Number of HSP's better than 10.0 without gapping: 32678
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33935
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
