Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004020A_C01 KMC004020A_c01
(491 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T09617 isoliquiritigenin 2'-O-methyltransferase - alfalfa g... 164 4e-40
dbj|BAA13683.1| O-methyltransferase [Glycyrrhiza echinata] 159 1e-38
pdb|1FPQ|A Chain A, Crystal Structure Analysis Of Selenomethioni... 157 8e-38
ref|NP_200227.1| O-methyltransferase 1; protein id: At5g54160.1,... 139 2e-32
gb|AAB96879.1| O-methyltransferase 1 [Arabidopsis thaliana] 137 6e-32
>pir||T09617 isoliquiritigenin 2'-O-methyltransferase - alfalfa
gi|13399462|pdb|1FP1|D Chain D, Crystal Structure
Analysis Of Chalcone O-Methyltransferase
gi|1843462|gb|AAB48059.1| isoliquiritigenin
2'-O-methyltransferase [Medicago sativa]
Length = 372
Score = 164 bits (416), Expect = 4e-40
Identities = 81/137 (59%), Positives = 99/137 (72%)
Frame = -3
Query: 489 NFDLPQVIQHASPHPGIEHVSGDMFESVPNGDAILFQRVCHNWPDEDCVKVLKICHKALP 310
NFDLPQVI++A P GIEHV GDMF SVP GDA++ + VCHNW DE C++ L CHKAL
Sbjct: 238 NFDLPQVIENAPPLSGIEHVGGDMFASVPQGDAMILKAVCHNWSDEKCIEFLSNCHKALS 297
Query: 309 EHGKVIIVDTIYPEVPNSSITSQCVSVVDNLMFLVHGAKERTENEFRSLCSSSGFSKFHV 130
+GKVIIV+ I PE PN+S S+ VS +DNLMF+ G +ERTE ++ L SGFSKF V
Sbjct: 298 PNGKVIIVEFILPEEPNTSEESKLVSTLDNLMFITVGGRERTEKQYEKLSKLSGFSKFQV 357
Query: 129 ASSTGSSSVIGVMEFYK 79
A +S +GVMEFYK
Sbjct: 358 ACRAFNS--LGVMEFYK 372
>dbj|BAA13683.1| O-methyltransferase [Glycyrrhiza echinata]
Length = 367
Score = 159 bits (403), Expect = 1e-38
Identities = 76/137 (55%), Positives = 98/137 (71%)
Frame = -3
Query: 489 NFDLPQVIQHASPHPGIEHVSGDMFESVPNGDAILFQRVCHNWPDEDCVKVLKICHKALP 310
NFDLPQVI++A P PGIE V GDMF SVP GDA++ + VCHNW DE C++ L CHKAL
Sbjct: 233 NFDLPQVIENAPPIPGIELVGGDMFASVPQGDAMILKAVCHNWSDEKCLEFLSNCHKALS 292
Query: 309 EHGKVIIVDTIYPEVPNSSITSQCVSVVDNLMFLVHGAKERTENEFRSLCSSSGFSKFHV 130
+GKVI+V+ I PE P + SQ S +DN+MF+ G +ERT+ ++ ++C +GFSKF V
Sbjct: 293 PNGKVIVVEFILPEEPEPTEESQLASTLDNIMFITVGGRERTQKQYENMCKLAGFSKFQV 352
Query: 129 ASSTGSSSVIGVMEFYK 79
A SS +GVMEFYK
Sbjct: 353 ACRAFSS--LGVMEFYK 367
>pdb|1FPQ|A Chain A, Crystal Structure Analysis Of Selenomethionine Substituted
Chalcone O-Methyltransferase
Length = 372
Score = 157 bits (396), Expect = 8e-38
Identities = 78/137 (56%), Positives = 95/137 (68%)
Frame = -3
Query: 489 NFDLPQVIQHASPHPGIEHVSGDMFESVPNGDAILFQRVCHNWPDEDCVKVLKICHKALP 310
NFDLPQVI++A P GIEHV GD F SVP GDA + + VCHNW DE C++ L CHKAL
Sbjct: 238 NFDLPQVIENAPPLSGIEHVGGDXFASVPQGDAXILKAVCHNWSDEKCIEFLSNCHKALS 297
Query: 309 EHGKVIIVDTIYPEVPNSSITSQCVSVVDNLMFLVHGAKERTENEFRSLCSSSGFSKFHV 130
+GKVIIV+ I PE PN+S S+ VS +DNL F+ G +ERTE ++ L SGFSKF V
Sbjct: 298 PNGKVIIVEFILPEEPNTSEESKLVSTLDNLXFITVGGRERTEKQYEKLSKLSGFSKFQV 357
Query: 129 ASSTGSSSVIGVMEFYK 79
A +S +GV EFYK
Sbjct: 358 ACRAFNS--LGVXEFYK 372
>ref|NP_200227.1| O-methyltransferase 1; protein id: At5g54160.1, supported by cDNA:
33790., supported by cDNA: gi_17065521, supported by
cDNA: gi_20148472, supported by cDNA: gi_2781393
[Arabidopsis thaliana] gi|24212073|sp|Q9FK25|OMT1_ARATH
Quercetin 3-O-methyltransferase 1 (AtOMT1) (Flavonol
3-O-methyltransferase 1) (Caffeic acid/5-hydroxyferulic
acid O-methyltransferase) gi|10178165|dbj|BAB11578.1|
O-methyltransferase [Arabidopsis thaliana]
gi|17065522|gb|AAL32915.1| O-methyltransferase
[Arabidopsis thaliana] gi|20148473|gb|AAM10127.1|
O-methyltransferase [Arabidopsis thaliana]
gi|21592899|gb|AAM64849.1| O-methyltransferase
[Arabidopsis thaliana]
Length = 363
Score = 139 bits (350), Expect = 2e-32
Identities = 70/136 (51%), Positives = 92/136 (67%), Gaps = 2/136 (1%)
Frame = -3
Query: 489 NFDLPQVIQHASPHPGIEHVSGDMFESVPNGDAILFQRVCHNWPDEDCVKVLKICHKALP 310
NFDLP VI+ A HPGIEHV GDMF SVP GDAI + +CH+W DE CVK LK C+++LP
Sbjct: 227 NFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLP 286
Query: 309 EHGKVIIVDTIYPEVPNSSITSQCVSVVDNLMFLVH--GAKERTENEFRSLCSSSGFSKF 136
E GKVI+ + I PE P+SS++++ V VD +M L H G KERTE EF +L +SGF
Sbjct: 287 EDGKVILAECILPETPDSSLSTKQVVHVDCIM-LAHNPGGKERTEKEFEALAKASGFKGI 345
Query: 135 HVASSTGSSSVIGVME 88
V ++I +++
Sbjct: 346 KVVCDAFGVNLIELLK 361
>gb|AAB96879.1| O-methyltransferase 1 [Arabidopsis thaliana]
Length = 363
Score = 137 bits (345), Expect = 6e-32
Identities = 69/136 (50%), Positives = 92/136 (66%), Gaps = 2/136 (1%)
Frame = -3
Query: 489 NFDLPQVIQHASPHPGIEHVSGDMFESVPNGDAILFQRVCHNWPDEDCVKVLKICHKALP 310
NF+LP VI+ A HPGIEHV GDMF SVP GDAI + +CH+W DE CVK LK C+++LP
Sbjct: 227 NFNLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLP 286
Query: 309 EHGKVIIVDTIYPEVPNSSITSQCVSVVDNLMFLVH--GAKERTENEFRSLCSSSGFSKF 136
E GKVI+ + I PE P+SS++++ V VD +M L H G KERTE EF +L +SGF
Sbjct: 287 EDGKVILAECILPETPDSSLSTKQVVHVDCIM-LAHNPGGKERTEKEFEALAKASGFKGI 345
Query: 135 HVASSTGSSSVIGVME 88
V ++I +++
Sbjct: 346 KVVCDAFGVNLIELLK 361
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 411,963,322
Number of Sequences: 1393205
Number of extensions: 8816070
Number of successful extensions: 20995
Number of sequences better than 10.0: 219
Number of HSP's better than 10.0 without gapping: 20227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20838
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 14203329973
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
