>KMC003997A_C01 KMC003997A_c01
gatgcagatGGAAGATAAAGTTTGTCACTATAACAAAAGTGCACTGCCGCTATTCATACG
TTGACATAAGAATTTAAAAATCATAAAAAGAAAAACCTTATCGGTGACTATTTCTAGGAA
GATTATCTAAAACATAATGTCCAAGGCGACCCGTACGGAAAAGATTAAGCAACTTCCTAG
CAATTTTGACATGAGCATCTTCTACACACTCAGTTGGAATGTGGAATGCTTCCTGCAAGG
CACTAAATTGCCTAGCTATAACTGCTGCCATTTCATCTTCACATCTTATATTGCCATCAA
AAGATGAAATTGTTTCATAAAGTGTTTGTCGAACATCCTGCACTATGCAATCCTGTGTGT
GATCTGTAGGGATTTGCTTTTTCTGCTTCATGTGTAACCCTGAGCTAGTCGAACGTTTTG
TTGTGCTGTTGAGGAATAATCCATCATTATCCTTGGCAGATAACTTTGCCCATTTCTTGT
ACTGCTGCTCACTTGAGTTGTGAATAGCTAGAAACTATTGAGCAATTTCTTTTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003997A_C01 KMC003997A_c01
         (534 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T01912 hypothetical protein T12H20.1 - Arabidopsis thaliana...   120  1e-26
gb|AAL86477.1|AC077693_16 putative GTPase domain containing prot...   108  5e-23
ref|NP_192803.1| putative protein; protein id: At4g10650.1 [Arab...    86  4e-16
pir||T00823 hypothetical protein At2g41670 [imported] - Arabidop...    74  1e-12
ref|NP_181698.2| unknown protein; protein id: At2g41670.1, suppo...    74  1e-12

>pir||T01912 hypothetical protein T12H20.1 - Arabidopsis thaliana
           gi|3600035|gb|AAC35523.1| contains similarity to
           GTP-binding proteins [Arabidopsis thaliana]
          Length = 377

 Score =  120 bits (300), Expect = 1e-26
 Identities = 65/137 (47%), Positives = 90/137 (65%)
 Frame = -3

Query: 529 EIAQ*FLAIHNSSEQQYKKWAKLSAKDNDGLFLNSTTKRSTSSGLHMKQKKQIPTDHTQD 350
           ++A+ FL I NSS + YKKWAKL    +    L+  + +S +     K K+Q  TDHTQD
Sbjct: 237 KLARLFLTILNSSHE-YKKWAKLCKSQDLTESLSDESSKSDA-----KHKRQYATDHTQD 290

Query: 349 CIVQDVRQTLYETISSFDGNIRCEDEMAAVIARQFSALQEAFHIPTECVEDAHVKIARKL 170
            IV DVR+ LYETIS+FDGN+  E  M  +I  QF+AL+    +P E  E A +++A K+
Sbjct: 291 FIVYDVRRVLYETISAFDGNLEDEISMGNLIETQFAALRSVLRVPEEASEFADLRVASKI 350

Query: 169 LNLFRTGRLGHYVLDNL 119
           LNL+RTGRLGHY L+++
Sbjct: 351 LNLYRTGRLGHYTLEHV 367

>gb|AAL86477.1|AC077693_16 putative GTPase domain containing protein [Oryza sativa (japonica
           cultivar-group)]
          Length = 370

 Score =  108 bits (269), Expect = 5e-23
 Identities = 64/138 (46%), Positives = 86/138 (61%)
 Frame = -3

Query: 529 EIAQ*FLAIHNSSEQQYKKWAKLSAKDNDGLFLNSTTKRSTSSGLHMKQKKQIPTDHTQD 350
           EIAQ  LAI NS E  YKKW  ++   +    + S +   +SS  H   K+Q  +DHTQD
Sbjct: 232 EIAQFLLAILNSRET-YKKWENMNQAGD----MPSFSHAMSSSSHH--NKRQYASDHTQD 284

Query: 349 CIVQDVRQTLYETISSFDGNIRCEDEMAAVIARQFSALQEAFHIPTECVEDAHVKIARKL 170
            +V+ VRQ L+++ISSF G +  E+E+ ++I  QF ALQEAF +  +  ED    +A KL
Sbjct: 285 FVVKAVRQVLFDSISSFKGYLENENELKSLIECQFIALQEAFRVSADLSEDVRKLVAMKL 344

Query: 169 LNLFRTGRLGHYVLDNLP 116
           LNL+RTGRLG Y LD  P
Sbjct: 345 LNLYRTGRLGRYTLDCAP 362

>ref|NP_192803.1| putative protein; protein id: At4g10650.1 [Arabidopsis thaliana]
           gi|7487636|pir||T04200 hypothetical protein T4F9.110 -
           Arabidopsis thaliana gi|4539443|emb|CAB40031.1| putative
           protein [Arabidopsis thaliana]
           gi|7267763|emb|CAB81166.1| putative protein [Arabidopsis
           thaliana]
          Length = 332

 Score = 85.5 bits (210), Expect = 4e-16
 Identities = 49/107 (45%), Positives = 66/107 (60%)
 Frame = -3

Query: 529 EIAQ*FLAIHNSSEQQYKKWAKLSAKDNDGLFLNSTTKRSTSSGLHMKQKKQIPTDHTQD 350
           ++A+ FL I NSS + YKKWAKL    +    L+  + +S +     K K+Q  TDHTQD
Sbjct: 214 KLARLFLTILNSSHE-YKKWAKLCKSQDLTESLSDESSKSDA-----KHKRQYATDHTQD 267

Query: 349 CIVQDVRQTLYETISSFDGNIRCEDEMAAVIARQFSALQEAFHIPTE 209
            IV DVR+ LYETIS+FDGN+  E  M  +I  QF+AL+    +P E
Sbjct: 268 FIVYDVRRVLYETISAFDGNLEDEISMGNLIETQFAALRSVLRVPEE 314

>pir||T00823 hypothetical protein At2g41670 [imported] - Arabidopsis thaliana
           gi|2618702|gb|AAB84349.1| unknown protein [Arabidopsis
           thaliana]
          Length = 391

 Score = 73.9 bits (180), Expect = 1e-12
 Identities = 50/142 (35%), Positives = 80/142 (56%), Gaps = 3/142 (2%)
 Frame = -3

Query: 532 KEIAQ*FLAIHNSSEQQYKKWAKLSAKDNDGLFLNSTTKRSTS-SGLHMKQKKQIPTD-- 362
           + IAQ FLAI N        W  L    N+G   +   K S +   L  ++ KQ  +   
Sbjct: 237 ERIAQYFLAILNIRGTPLH-WKYLVEGINEGPHADCIDKPSYNLKDLRHQRTKQPDSSAL 295

Query: 361 HTQDCIVQDVRQTLYETISSFDGNIRCEDEMAAVIARQFSALQEAFHIPTECVEDAHVKI 182
           H    ++ +V+++LY T+S FDG+   E+++  +I +QF  LQ+A  IP +  E A + +
Sbjct: 296 HYVGDMISEVQRSLYITLSEFDGDTEDENDLECLIEQQFEVLQKALKIPHKASE-ARLMV 354

Query: 181 ARKLLNLFRTGRLGHYVLDNLP 116
           ++K L LFRTGRLG ++LD++P
Sbjct: 355 SKKFLTLFRTGRLGPFILDDVP 376

>ref|NP_181698.2| unknown protein; protein id: At2g41670.1, supported by cDNA:
           gi_20466543 [Arabidopsis thaliana]
           gi|20466544|gb|AAM20589.1| unknown protein [Arabidopsis
           thaliana] gi|23198342|gb|AAN15698.1| unknown protein
           [Arabidopsis thaliana]
          Length = 386

 Score = 73.9 bits (180), Expect = 1e-12
 Identities = 50/142 (35%), Positives = 80/142 (56%), Gaps = 3/142 (2%)
 Frame = -3

Query: 532 KEIAQ*FLAIHNSSEQQYKKWAKLSAKDNDGLFLNSTTKRSTS-SGLHMKQKKQIPTD-- 362
           + IAQ FLAI N        W  L    N+G   +   K S +   L  ++ KQ  +   
Sbjct: 232 ERIAQYFLAILNIRGTPLH-WKYLVEGINEGPHADCIDKPSYNLKDLRHQRTKQPDSSAL 290

Query: 361 HTQDCIVQDVRQTLYETISSFDGNIRCEDEMAAVIARQFSALQEAFHIPTECVEDAHVKI 182
           H    ++ +V+++LY T+S FDG+   E+++  +I +QF  LQ+A  IP +  E A + +
Sbjct: 291 HYVGDMISEVQRSLYITLSEFDGDTEDENDLECLIEQQFEVLQKALKIPHKASE-ARLMV 349

Query: 181 ARKLLNLFRTGRLGHYVLDNLP 116
           ++K L LFRTGRLG ++LD++P
Sbjct: 350 SKKFLTLFRTGRLGPFILDDVP 371

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 422,054,638
Number of Sequences: 1393205
Number of extensions: 8351928
Number of successful extensions: 18012
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 17629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18007
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17885181664
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf094f08 BP074349 1 474 2 MR033a12_f BP078514 10 400 3 MPDL021c04_f AV777546 11 316 4 SPDL022e06_f BP053379 27 534

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