>KMC003993A_C01 KMC003993A_c01
gagcatgctagattttttcAACCAAATAGGGAAAAGATAGCATGCAACATACTTTTTTTT
CAGAGAACTTCTACAGGGTCTTTATACATAATAACCAGAAGAAATAGCATACCAAAATTT
CTTGATTACACCCTTAATGATGTTCACTATGCTTAGTGCTTACATACCCTTAATAAACAA
ATAATTTTCTGGTGATCATCTTCTCCTTACTAAGATCCCACTTTTCAAAAGAAAAAAAAA
AATCATGCTTCCTGTACTTGCTTTCAACCCCCCTCTCTATTTGGGGATTTTTTGGTTCAA
ATCTTTCTGATTGCACATACTCTCACTACTTGGGAATATAACCCATGGGAACTGCTTCGG
GATCGAAGTGTTGTGATGATCATAGAAAGAGCCACCATAATCCAAAGGTTTGATAACTAC
AGACTCCTGCTTCCCATCCTGGCTCCTGGCACTGCCCCAAATAATATCTCATGGGTTTGC
TCATGGCTTTGCTCATAGAAAGTCTGAGGAATGGATGAATGGTACTGATGGCGTTGATGG
TGATGCTTAGGTTGCTGTAGTTGCTGCTGCTGCTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003993A_C01 KMC003993A_c01
         (575 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_177185.2| unknown protein; protein id: At1g70280.1, suppo...    54  2e-06
pir||T01495 hypothetical protein F17O7.19 - Arabidopsis thaliana...    54  2e-06
ref|NP_173800.2| unknown protein; protein id: At1g23880.1, suppo...    50  2e-05
gb|AAF87138.1|AC002423_3 T23E23.5 [Arabidopsis thaliana]               50  2e-05
ref|NP_648226.2| CG7015-PA [Drosophila melanogaster] gi|23093910...    39  0.061

>ref|NP_177185.2| unknown protein; protein id: At1g70280.1, supported by cDNA:
           gi_17065223 [Arabidopsis thaliana]
           gi|17065224|gb|AAL32766.1| Unknown protein [Arabidopsis
           thaliana] gi|21387163|gb|AAM47985.1| unknown protein
           [Arabidopsis thaliana]
          Length = 447

 Score = 53.5 bits (127), Expect = 2e-06
 Identities = 26/48 (54%), Positives = 34/48 (70%), Gaps = 6/48 (12%)
 Frame = -1

Query: 575 QQQQQLQQPKHHHQRHQ------YHSSIPQTFYEQSHEQTHEILFGAV 450
           QQQQQ Q  KH HQ+ Q      ++SSIP T+YEQ  E+++EI+FGAV
Sbjct: 353 QQQQQKQHQKHQHQQQQQQQHRRHYSSIPHTYYEQDSEKSNEIVFGAV 400

>pir||T01495 hypothetical protein F17O7.19 - Arabidopsis thaliana
           gi|3176691|gb|AAC18814.1| Contains homology to
           serine/threonine protein kinase gb|X99618 from
           Mycobacterium tuberculosis.  ESTs gb|F14403, gb|F14404,
           and gb|N96730 come from this gene. [Arabidopsis
           thaliana]
          Length = 493

 Score = 53.5 bits (127), Expect = 2e-06
 Identities = 26/48 (54%), Positives = 34/48 (70%), Gaps = 6/48 (12%)
 Frame = -1

Query: 575 QQQQQLQQPKHHHQRHQ------YHSSIPQTFYEQSHEQTHEILFGAV 450
           QQQQQ Q  KH HQ+ Q      ++SSIP T+YEQ  E+++EI+FGAV
Sbjct: 399 QQQQQKQHQKHQHQQQQQQQHRRHYSSIPHTYYEQDSEKSNEIVFGAV 446

>ref|NP_173800.2| unknown protein; protein id: At1g23880.1, supported by cDNA:
           gi_18700090, supported by cDNA: gi_20856012 [Arabidopsis
           thaliana] gi|18700091|gb|AAL77657.1| At1g23880/T23E23_8
           [Arabidopsis thaliana] gi|20856013|gb|AAM26643.1|
           At1g23880/T23E23_8 [Arabidopsis thaliana]
          Length = 545

 Score = 50.4 bits (119), Expect = 2e-05
 Identities = 21/37 (56%), Positives = 28/37 (74%)
 Frame = -1

Query: 566 QQLQQPKHHHQRHQYHSSIPQTFYEQSHEQTHEILFG 456
           Q+ QQ KH    H++HS+IP T YEQS E+T+EI+FG
Sbjct: 479 QEQQQEKHQQYHHRHHSTIPYTLYEQSSEKTNEIVFG 515

>gb|AAF87138.1|AC002423_3 T23E23.5 [Arabidopsis thaliana]
          Length = 493

 Score = 50.4 bits (119), Expect = 2e-05
 Identities = 21/37 (56%), Positives = 28/37 (74%)
 Frame = -1

Query: 566 QQLQQPKHHHQRHQYHSSIPQTFYEQSHEQTHEILFG 456
           Q+ QQ KH    H++HS+IP T YEQS E+T+EI+FG
Sbjct: 427 QEQQQEKHQQYHHRHHSTIPYTLYEQSSEKTNEIVFG 463

>ref|NP_648226.2| CG7015-PA [Drosophila melanogaster] gi|23093910|gb|AAF50415.2|
           CG7015-PA [Drosophila melanogaster]
          Length = 1039

 Score = 38.5 bits (88), Expect = 0.061
 Identities = 18/36 (50%), Positives = 22/36 (61%)
 Frame = -1

Query: 575 QQQQQLQQPKHHHQRHQYHSSIPQTFYEQSHEQTHE 468
           QQQQQ QQ  HHHQ  Q H  + Q   +Q H+Q H+
Sbjct: 73  QQQQQHQQNMHHHQHQQQH--MQQQQQQQQHQQQHQ 106

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 522,025,028
Number of Sequences: 1393205
Number of extensions: 11824955
Number of successful extensions: 47074
Number of sequences better than 10.0: 286
Number of HSP's better than 10.0 without gapping: 33833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42764
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21530810025
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf094c05 BP074314 1 392 2 SPD014a06_f BP045081 100 483 3 MPD016b11_f AV771079 120 589 4 MFBL002e08_f BP041377 122 327 5 MPDL092a09_f AV781287 136 655 6 GNf097a12 BP074531 148 534 7 MFB026h06_f BP035920 208 506

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