>KMC003989A_C01 KMC003989A_c01
ggccccccctttgtgtagAGGATTCAACATATATATGACAAAAAGAATTACATCAATGCA
AAAGTGTTGCTTTATCAAAGCTGGACAAATACATCACCTTGTACAGACAAATTCTAAGAA
CACAGCAAACAACATGTCTAAGAACACAAGCAAACAACATGTACCGACAGAAGTTTAGTT
GGAAAAGTTCAATGGGGTGCCAAAAGAAGTTAATATATTAGAAAAGCTTGCAAATTTAGA
GAGAGCTTGATGTGGAGAACGGGAAAAAGCAACAGACCGAAGAAGGAAAGGAAATTTGTG
CCTTTCCTGGAGCATGAAGCTAGATGCAACTCAACAAGATCTGGAACAGTTGTGCCTTTC
CTTTACCTTCATCATTCATGAACCCTGAACCCAAATTGTATGAGTTGCTCTTCAAATGGT
AACCCAGAAAACCCAAATCGAAAACTATATTTATCAAAGATGAACTAGGACTGATAAATA
AAGAAAAACTGGAATTGGAATTTTTTTCTTGCAAATCTGGAACATCCCCTTCAACAACCA
CCTTCCAACTTAGAGCAGGCCCTGGCATGACAAGAAGGAGTATCAACAACACTCCAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003989A_C01 KMC003989A_c01
         (597 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAA24215.1| glycosyl transferase                                    33  2.1
pir||A42718 polysialyltransferase - Escherichia coli (strain K92)      33  2.1
ref|NP_187419.1| Exostosin family; protein id: At3g07620.1 [Arab...    33  2.1
ref|NP_014552.1| involved in salt tolerance; Hal9p [Saccharomyce...    33  2.8
pir||A61542 poly-alpha-2,8 sialosyl sialyltransferase (EC 2.4.-....    32  4.7

>gb|AAA24215.1| glycosyl transferase
          Length = 409

 Score = 33.5 bits (75), Expect = 2.1
 Identities = 26/65 (40%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
 Frame = +3

Query: 327 QLNKIW-NSCAFPLPSSFMNPEPKLYELLFKW*PRKPKSKTIFIKDELGLINKEKLELEF 503
           +L K++ N   F   S F N + K  ELL    P K KSK IFI   LG + K +L ++ 
Sbjct: 9   KLRKLFVNPIGFFRDSWFFNSKNKAEELLS---PLKIKSKNIFIVAHLGQLKKAELFIQK 65

Query: 504 FSCKS 518
           FS +S
Sbjct: 66  FSRRS 70

>pir||A42718 polysialyltransferase - Escherichia coli (strain K92)
          Length = 409

 Score = 33.5 bits (75), Expect = 2.1
 Identities = 26/65 (40%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
 Frame = +3

Query: 327 QLNKIW-NSCAFPLPSSFMNPEPKLYELLFKW*PRKPKSKTIFIKDELGLINKEKLELEF 503
           +L K++ N   F   S F N + K  ELL    P K KSK IFI   LG + K +L ++ 
Sbjct: 9   KLRKLFVNPIGFFRDSWFFNSKNKAEELLS---PLKIKSKNIFIVAHLGQLKKAELFIQK 65

Query: 504 FSCKS 518
           FS +S
Sbjct: 66  FSRRS 70

>ref|NP_187419.1| Exostosin family; protein id: At3g07620.1 [Arabidopsis thaliana]
           gi|6466945|gb|AAF13080.1|AC009176_7 hypothetical protein
           [Arabidopsis thaliana]
          Length = 470

 Score = 33.5 bits (75), Expect = 2.1
 Identities = 23/64 (35%), Positives = 31/64 (47%), Gaps = 11/64 (17%)
 Frame = -3

Query: 505 KNSNSSFSLFISPSSSLINIVFDLGFLGYHL-----------KSNSYNLGSGFMNDEGKG 359
           K+SNSS  L+ S SSSL    F   F+   +           K N  N GSG+   +GK 
Sbjct: 29  KDSNSSSHLYFSTSSSLWTSSFSPAFITVSIFLTVHRFREKRKRNGSNPGSGYWKRDGKV 88

Query: 358 KAQL 347
           +A+L
Sbjct: 89  EAEL 92

>ref|NP_014552.1| involved in salt tolerance; Hal9p [Saccharomyces cerevisiae]
           gi|1362355|pir||S57380 probable membrane protein YOL089c
           - yeast (Saccharomyces cerevisiae)
           gi|600469|emb|CAA58190.1| orf 00938 [Saccharomyces
           cerevisiae] gi|1419932|emb|CAA99101.1| ORF YOL089c
           [Saccharomyces cerevisiae]
          Length = 1030

 Score = 33.1 bits (74), Expect = 2.8
 Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
 Frame = +2

Query: 92  HHLVQTNSKNTANNMSKNTSKQHVPTEV*LEKFNG-VPKEVNILEKLANLE 241
           HH     S  T+N++S   +   VP     E  NG  P E NI++K++N++
Sbjct: 185 HHATPGESLQTSNSISNPVASSSVPNSGRFELLNGNSPLESNIIDKVSNIQ 235

>pir||A61542 poly-alpha-2,8 sialosyl sialyltransferase (EC 2.4.-.-) -
           Escherichia coli gi|42116|emb|CAA43053.1|
           polysialyltransferase [Escherichia coli]
           gi|146949|gb|AAA24213.1| glycosyl transferase
          Length = 409

 Score = 32.3 bits (72), Expect = 4.7
 Identities = 30/89 (33%), Positives = 41/89 (45%), Gaps = 1/89 (1%)
 Frame = +3

Query: 327 QLNKIW-NSCAFPLPSSFMNPEPKLYELLFKW*PRKPKSKTIFIKDELGLINKEKLELEF 503
           +L K++ N   F   S F N + K  ELL    P K KSK IFI   LG + K +  ++ 
Sbjct: 9   KLRKLFVNPIGFFRDSWFFNSKNKAEELLS---PLKIKSKNIFIISNLGQLKKAESFVQK 65

Query: 504 FSCKSGTSPSTTTFQLRAGPGMTRRSINN 590
           FS +S       T +    P +    INN
Sbjct: 66  FSKRSNYLIVLATEKNTEMPKIIVEQINN 94

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 538,154,655
Number of Sequences: 1393205
Number of extensions: 11781029
Number of successful extensions: 29735
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 28706
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29725
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23140425222
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD028b05_f AV771894 1 599 2 MRL009a06_f BP084133 5 468 3 MWM047e05_f AV765415 48 518 4 GNf094a03 BP074291 51 454

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