>KMC003985A_C01 KMC003985A_c01
tctaattgggctttaatctattttctcaagaatacttgaacagagcataatctttttcta
cagttcattctAGATGAAGGAGTAAACGTGGGATTGATTTCACTACATGCTATACTCCTA
ATGCGAAGCTTACATTAATTCAGTCGATGCATTTTCTCATTGGAATACAAATCAAACTAA
AACACATTCCAATAAATAAAACAGAAAGAAAATCGGGAAAAACATAGCAGAATTACATAA
AACGATGGTTGAGTAGGGAAGTTTAACAAATTCGAAACACACGGGATGAAATCTGCAACG
AAGAAACCCTGATTGCATATTGATCTTGACAAGACAAAATATGGAAGAGGTTACACTAAT
CATTTCAATCTTTTTGGTATCAACACCTCTCCAGAGATCCACCCACAGGGTATGTTGTAT
CTAATAAATGAAAACAGTAAACGAAGATTGAAAGAAACCCAACTTCCCAATGGCAATAAA
ATCAGGCAGATATCAGTCCATCTTTCACCAGAGAAGATCCAAACAGTGCCTTGACATCCA
TAAGCAAATTCTAAGGATTAGACCCATTTTTAACTCGAGTACCTTTGTCTGGCTTTAAAG
CTCTAGGATCAAGTGGGATGCTCCCAACCTTCAAAAAACCAGAAGACTCACTGAATCCAT
TAACTCTGACTTGCATTGAGCCTACTGTGACAACGTCATTTGGTTCCTTATTAGCAACAC
CATTGCTGTATGGTTTAGTCTCGGTCTGTTTTGGGGTAATGGTGACTGAACCAATCTTTA
AGACAGAAGAAATATCTTCCC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003985A_C01 KMC003985A_c01
         (801 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_175245.1| unknown protein; protein id: At1g48110.1 [Arabi...    35  1.3
gb|AAF79522.1|AC023673_10 F21D18.17 [Arabidopsis thaliana]             35  1.3
gb|EAA17233.1| hypothetical protein [Plasmodium yoelii yoelii]         35  1.7
gb|AAO51998.1| similar to Leishmania major. Ppg3 [Dictyostelium ...    33  3.7
emb|CAD53105.1| hypothetical protein, unlikely [Trypanosoma brucei]    33  4.9

>ref|NP_175245.1| unknown protein; protein id: At1g48110.1 [Arabidopsis thaliana]
          Length = 639

 Score = 35.0 bits (79), Expect = 1.3
 Identities = 29/88 (32%), Positives = 41/88 (45%), Gaps = 19/88 (21%)
 Frame = -3

Query: 799 EDISSVLK-IGSVTITPK----------------QTETKPYSNGVANKEPNDVVTVGSMQ 671
           ED SS  K I S+TI P                 Q ++KP S+G   K     V   S+ 
Sbjct: 551 EDTSSTQKKISSLTIDPSGTDSNPTTVSHLNQKSQAKSKPNSSGSLKKTDPSEVVDASLS 610

Query: 670 VRVNGF--SESSGFLKVGSIPLDPRALK 593
            + + F  + S   L VG+IPLDP++L+
Sbjct: 611 DKNDSFKVTGSPAILTVGTIPLDPKSLQ 638

>gb|AAF79522.1|AC023673_10 F21D18.17 [Arabidopsis thaliana]
          Length = 664

 Score = 35.0 bits (79), Expect = 1.3
 Identities = 29/88 (32%), Positives = 41/88 (45%), Gaps = 19/88 (21%)
 Frame = -3

Query: 799 EDISSVLK-IGSVTITPK----------------QTETKPYSNGVANKEPNDVVTVGSMQ 671
           ED SS  K I S+TI P                 Q ++KP S+G   K     V   S+ 
Sbjct: 576 EDTSSTQKKISSLTIDPSGTDSNPTTVSHLNQKSQAKSKPNSSGSLKKTDPSEVVDASLS 635

Query: 670 VRVNGF--SESSGFLKVGSIPLDPRALK 593
            + + F  + S   L VG+IPLDP++L+
Sbjct: 636 DKNDSFKVTGSPAILTVGTIPLDPKSLQ 663

>gb|EAA17233.1| hypothetical protein [Plasmodium yoelii yoelii]
          Length = 205

 Score = 34.7 bits (78), Expect = 1.7
 Identities = 18/49 (36%), Positives = 26/49 (52%)
 Frame = +1

Query: 352 YTNHFNLFGINTSPEIHPQGMLYLINENSKRRLKETQLPNGNKIRQISV 498
           YTNHF LF  N S EI  Q  +  I+ + K++ K+  L   N  R + +
Sbjct: 77  YTNHFQLFEYNKSEEIDKQKYINSISRSIKKQKKKMYLLEKNACRILDI 125

>gb|AAO51998.1| similar to Leishmania major. Ppg3 [Dictyostelium discoideum]
          Length = 642

 Score = 33.5 bits (75), Expect = 3.7
 Identities = 26/84 (30%), Positives = 36/84 (41%), Gaps = 3/84 (3%)
 Frame = +3

Query: 486 ADISPSFTREDPNSALTSISKF*GLDPFLTRVPLS---GFKALGSSGMLPTFKKPEDSLN 656
           +D  PSF    P+ +  ++S F G  P  +  PLS   G     SSG  P+     D  +
Sbjct: 350 SDSLPSFPGSFPSGSSDTLSSFPGSLPSDSSDPLSSSPGSLPSDSSGSFPSLTGSSDLSS 409

Query: 657 PLTLTCIEPTVTTSFGSLLATPLL 728
            +T T   PT T +F      P L
Sbjct: 410 TITTTPTTPTTTPNFTFTPTIPTL 433

>emb|CAD53105.1| hypothetical protein, unlikely [Trypanosoma brucei]
          Length = 99

 Score = 33.1 bits (74), Expect = 4.9
 Identities = 18/35 (51%), Positives = 25/35 (71%), Gaps = 2/35 (5%)
 Frame = -1

Query: 261 LPYSTIVL-CNSAMFFPIFFLFYLLECV-LV*FVF 163
           +P+ TI+L C    FFP+FFLF+L E + +V FVF
Sbjct: 49  IPHLTILLLCCCCCFFPLFFLFFLGEAINVVLFVF 83

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 665,457,681
Number of Sequences: 1393205
Number of extensions: 14382575
Number of successful extensions: 32853
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 31603
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32841
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 40616159090
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR062f04_f BP080766 1 472 2 MRL028b03_f BP085122 72 499 3 MRL016g02_f BP084546 157 213 4 MR011b11_f BP076766 163 518 5 MPDL021c07_f AV777547 198 562 6 GNf093e06 BP074251 215 576 7 SPDL028c03_f BP053722 275 804

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