>KMC003903A_C02 KMC003903A_c02
gtgatcaaatcatcagggacaaactAGAGCAAACTGGAGGAAACATTATATTCATTAATG
CACCTAACACTTGAGGTATACAAGTTTTGATAACTAATATACAGTCTGGATCTTAATGGA
ATATGAAATATGACCAGCAATAACACACCAAAAAATCCCTTTTAGCTTCTGGTGGAACTG
TGGAAGTGATAAATGTTAAAATAAACAGATTTGTGGAGTACAGAAGCAATAGAATCCTTG
CTTCCTAACTCATACAGCAGTTGTTCCCTCTTCTTGTCCAGTACCTTTATCATCATCACT
ATCCTGGCCATGAATTGCATTCTGTAAAAACTTATGTTCAGAGGGCATATTTTTCCTCGC
TTTCCGAGTTCCTTCAACTTTCCATAGATCCCAAACACGAGTTTTTGGAGGTGCCGATGC
TTCTGCCGTCGGTGCAATTAATCCTGGTTTTCCCTCATCAATAACACCATCAACTAAACC
ATATTCCTTGGCTTCCCAAGGATTCATAAAGTTATCGCGATCAGTGTCTAATTCAATCTG
CTCTTCAGGTTTCCCTGTAATTCGTGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003903A_C02 KMC003903A_c02
         (567 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_564880.1| ATP-dependent Clp protease proteolytic subunit ...   137  7e-32
pir||T52041 probable ATP-dependent clp proteinase (EC 3.4.21.-) ...   137  7e-32
gb|AAK48955.1|AF370528_1 ATP-dependent Clp protease; nClpP3 [Ara...   136  2e-31
emb|CAC80640.1| ClpP putative protein [Brassica napus]                127  1e-28
gb|AAL34333.1|L41144_1 ClpP [Brassica oleracea]                       127  1e-28

>ref|NP_564880.1| ATP-dependent Clp protease proteolytic subunit (ClpP3); protein id:
           At1g66670.1, supported by cDNA: 34480., supported by
           cDNA: gi_13899066, supported by cDNA: gi_18377549,
           supported by cDNA: gi_5360590 [Arabidopsis thaliana]
           gi|25453514|pir||T52453 ATP-dependent Clp proteinase (EC
           3.4.21.-) catalytic chain P 3 [imported] - Arabidopsis
           thaliana gi|5360591|dbj|BAA82067.1| nClpP3 [Arabidopsis
           thaliana] gi|12322291|gb|AAG51173.1|AC079285_6
           ATP-dependent Clp protease (nClpP3) [Arabidopsis
           thaliana] gi|12597762|gb|AAG60075.1|AC013288_9
           ATP-dependent Clp protease (nClpP3) [Arabidopsis
           thaliana] gi|21592949|gb|AAM64899.1| ATP-dependent Clp
           protease proteolytic subunit ClpP3 [Arabidopsis
           thaliana]
          Length = 309

 Score =  137 bits (346), Expect = 7e-32
 Identities = 60/83 (72%), Positives = 69/83 (82%)
 Frame = -1

Query: 567 SRITGKPEEQIELDTDRDNFMNPWEAKEYGLVDGVIDEGKPGLIAPTAEASAPPKTRVWD 388
           SRITGKPE +IE DTDRDNF+NPWEAKEYGL+D VID+GKPGLIAP  + + PPKT+VWD
Sbjct: 221 SRITGKPESEIESDTDRDNFLNPWEAKEYGLIDAVIDDGKPGLIAPIGDGTPPPKTKVWD 280

Query: 387 LWKVEGTRKARKNMPSEHKFLQN 319
           LWKVEGT+K   N+PSE    QN
Sbjct: 281 LWKVEGTKKDNTNLPSERSMTQN 303

>pir||T52041 probable ATP-dependent clp proteinase (EC 3.4.21.-) [imported] -
           Arabidopsis thaliana (fragment)
           gi|3599968|gb|AAC35489.1| clp protease [Arabidopsis
           thaliana]
          Length = 310

 Score =  137 bits (346), Expect = 7e-32
 Identities = 60/83 (72%), Positives = 69/83 (82%)
 Frame = -1

Query: 567 SRITGKPEEQIELDTDRDNFMNPWEAKEYGLVDGVIDEGKPGLIAPTAEASAPPKTRVWD 388
           SRITGKPE +IE DTDRDNF+NPWEAKEYGL+D VID+GKPGLIAP  + + PPKT+VWD
Sbjct: 222 SRITGKPESEIESDTDRDNFLNPWEAKEYGLIDAVIDDGKPGLIAPIGDGTPPPKTKVWD 281

Query: 387 LWKVEGTRKARKNMPSEHKFLQN 319
           LWKVEGT+K   N+PSE    QN
Sbjct: 282 LWKVEGTKKDNTNLPSERSMTQN 304

>gb|AAK48955.1|AF370528_1 ATP-dependent Clp protease; nClpP3 [Arabidopsis thaliana]
           gi|18377550|gb|AAL66941.1| ATP-dependent Clp protease
           (nClpP3) [Arabidopsis thaliana]
          Length = 309

 Score =  136 bits (342), Expect = 2e-31
 Identities = 59/83 (71%), Positives = 69/83 (83%)
 Frame = -1

Query: 567 SRITGKPEEQIELDTDRDNFMNPWEAKEYGLVDGVIDEGKPGLIAPTAEASAPPKTRVWD 388
           SRITGKPE +IE DTDRDNF+NPWEAKEYGL+D VID+GKPGLIAP  + + PPKT+VWD
Sbjct: 221 SRITGKPESEIESDTDRDNFLNPWEAKEYGLIDAVIDDGKPGLIAPIGDGTPPPKTKVWD 280

Query: 387 LWKVEGTRKARKNMPSEHKFLQN 319
           LWKVEGT++   N+PSE    QN
Sbjct: 281 LWKVEGTKEDNTNLPSERSMTQN 303

>emb|CAC80640.1| ClpP putative protein [Brassica napus]
          Length = 313

 Score =  127 bits (319), Expect = 1e-28
 Identities = 55/83 (66%), Positives = 67/83 (80%)
 Frame = -1

Query: 567 SRITGKPEEQIELDTDRDNFMNPWEAKEYGLVDGVIDEGKPGLIAPTAEASAPPKTRVWD 388
           SRITGKPE +IE DTDRD F+NPWEAKEYGLVD VID+GKPGL+AP  + + PP T+VWD
Sbjct: 225 SRITGKPESEIEGDTDRDYFLNPWEAKEYGLVDAVIDDGKPGLVAPIGDGTPPPNTKVWD 284

Query: 387 LWKVEGTRKARKNMPSEHKFLQN 319
            WKVEGT+   K++P+E   +QN
Sbjct: 285 FWKVEGTKNDNKDLPTEQSRVQN 307

>gb|AAL34333.1|L41144_1 ClpP [Brassica oleracea]
          Length = 279

 Score =  127 bits (319), Expect = 1e-28
 Identities = 55/83 (66%), Positives = 67/83 (80%)
 Frame = -1

Query: 567 SRITGKPEEQIELDTDRDNFMNPWEAKEYGLVDGVIDEGKPGLIAPTAEASAPPKTRVWD 388
           SRITGKPE +IE DTDRD F+NPWEAKEYGLVD VID+GKPGL+AP  + + PP T+VWD
Sbjct: 191 SRITGKPESEIEGDTDRDYFLNPWEAKEYGLVDAVIDDGKPGLVAPIGDGTPPPNTKVWD 250

Query: 387 LWKVEGTRKARKNMPSEHKFLQN 319
            WKVEGT+   K++P+E   +QN
Sbjct: 251 FWKVEGTKNDNKDLPTEQSRVQN 273

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 489,519,877
Number of Sequences: 1393205
Number of extensions: 10595272
Number of successful extensions: 32024
Number of sequences better than 10.0: 260
Number of HSP's better than 10.0 without gapping: 31080
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32001
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20669577624
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR020h10_f BP077558 1 481 2 MF091g07_f BP033088 26 470 3 SPDL035e01_f BP054201 36 544 4 MFB019d05_f BP035336 36 569 5 GNf085b05 BP073618 37 440

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