>KMC003899A_C01 KMC003899A_c01
acgggcccccctAAAATGAAGAATCTATCTCTTTTAGTGGCGATTCTCTGTGCGATTTCC
CTCCACTCAGTCGCTGCTACTTCCAGCGCCTATCCTACCACCCCTGGTCTCGATTCCGGC
GACTGCACCCTCGCCGGCGGCGACAGTCTTCTCGTCCCTCCACGGCGAGAAGTTTACGAT
GACGCCGGAATCTACGACATCACCCACCGGTACGTGCCTGAGATGCCGGTGTGGAACTCC
AAAGAGGGGTTAGGGCATTTCGTGTGGCTTGCCCAGAGCATGAAGAATGGCTCATGGGCT
AACGGCTCAGAAATGAAGCTCGGTGTTCACACTGGTACCCATGTCGACGCGCCCAGCCAC
TTCTATGACAATTACCTAGACGCCGGCTTCGACGTCGATACACTCGACCTAAGAGTCCTC
AACGGACTTGCACTTTTGATTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003899A_C01 KMC003899A_c01
         (442 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T06135 hypothetical protein F23E12.220 - Arabidopsis thalia...   163  5e-40
ref|NP_567979.1| putative protein; protein id: At4g35220.1, supp...   163  5e-40
pir||T05418 hypothetical protein F28A23.60 - Arabidopsis thalian...   141  2e-33
ref|NP_567957.1| putative protein; protein id: At4g34180.1, supp...   141  2e-33
ref|NP_175091.1| hypothetical protein; protein id: At1g44542.1 [...   134  3e-31

>pir||T06135 hypothetical protein F23E12.220 - Arabidopsis thaliana
           gi|3080428|emb|CAA18747.1| putative protein [Arabidopsis
           thaliana] gi|7270474|emb|CAB80239.1| putative protein
           [Arabidopsis thaliana]
          Length = 180

 Score =  163 bits (412), Expect = 5e-40
 Identities = 83/139 (59%), Positives = 97/139 (69%)
 Frame = +1

Query: 25  LSLLVAILCAISLHSVAATSSAYPTTPGLDSGDCTLAGGDSLLVPPRREVYDDAGIYDIT 204
           L  L+ +L   SL   A  S+AYP+ PG    D    G    L P RREVY +  IYDI+
Sbjct: 6   LFFLLTLLSLPSLLISAGASNAYPSIPGTAPID---GGFTDELKPIRREVYGNGKIYDIS 62

Query: 205 HRYVPEMPVWNSKEGLGHFVWLAQSMKNGSWANGSEMKLGVHTGTHVDAPSHFYDNYLDA 384
           HRY PEMP W+S EG+G F+WLA SMKNGS AN SEMK+  HTGTHVD+P H YD Y DA
Sbjct: 63  HRYTPEMPSWDSSEGIGRFLWLAASMKNGSLANNSEMKIPTHTGTHVDSPGHVYDKYYDA 122

Query: 385 GFDVDTLDLRVLNGLALLI 441
           GFDVD+LDL+VLNGLALL+
Sbjct: 123 GFDVDSLDLQVLNGLALLV 141

>ref|NP_567979.1| putative protein; protein id: At4g35220.1, supported by cDNA:
           1368., supported by cDNA: gi_13937203, supported by
           cDNA: gi_18491126 [Arabidopsis thaliana]
           gi|13937204|gb|AAK50095.1|AF372956_1
           AT4g35220/F23E12_220 [Arabidopsis thaliana]
           gi|18491127|gb|AAL69532.1| AT4g35220/F23E12_220
           [Arabidopsis thaliana] gi|21537400|gb|AAM61741.1|
           unknown [Arabidopsis thaliana]
          Length = 272

 Score =  163 bits (412), Expect = 5e-40
 Identities = 83/139 (59%), Positives = 97/139 (69%)
 Frame = +1

Query: 25  LSLLVAILCAISLHSVAATSSAYPTTPGLDSGDCTLAGGDSLLVPPRREVYDDAGIYDIT 204
           L  L+ +L   SL   A  S+AYP+ PG    D    G    L P RREVY +  IYDI+
Sbjct: 6   LFFLLTLLSLPSLLISAGASNAYPSIPGTAPID---GGFTDELKPIRREVYGNGKIYDIS 62

Query: 205 HRYVPEMPVWNSKEGLGHFVWLAQSMKNGSWANGSEMKLGVHTGTHVDAPSHFYDNYLDA 384
           HRY PEMP W+S EG+G F+WLA SMKNGS AN SEMK+  HTGTHVD+P H YD Y DA
Sbjct: 63  HRYTPEMPSWDSSEGIGRFLWLAASMKNGSLANNSEMKIPTHTGTHVDSPGHVYDKYYDA 122

Query: 385 GFDVDTLDLRVLNGLALLI 441
           GFDVD+LDL+VLNGLALL+
Sbjct: 123 GFDVDSLDLQVLNGLALLV 141

>pir||T05418 hypothetical protein F28A23.60 - Arabidopsis thaliana
           gi|2911044|emb|CAA17554.1| putative protein [Arabidopsis
           thaliana] gi|7270368|emb|CAB80135.1| putative protein
           [Arabidopsis thaliana]
          Length = 352

 Score =  141 bits (355), Expect = 2e-33
 Identities = 68/96 (70%), Positives = 78/96 (80%), Gaps = 1/96 (1%)
 Frame = +1

Query: 157 PPRREVYDDAGIYDITHRYVPEMPVWNSKEGLGH-FVWLAQSMKNGSWANGSEMKLGVHT 333
           P RREVY+   IYDI+HRY PE+P W S EGLG  F+ LA SMKNGS+AN SEMKL VH+
Sbjct: 29  PIRREVYEGGKIYDISHRYTPEIPAWESSEGLGKTFLRLAASMKNGSFANVSEMKLSVHS 88

Query: 334 GTHVDAPSHFYDNYLDAGFDVDTLDLRVLNGLALLI 441
           GTHVDAP HF+DNY DAGFD D+LDL+VLNG ALL+
Sbjct: 89  GTHVDAPGHFWDNYYDAGFDTDSLDLQVLNGPALLV 124

>ref|NP_567957.1| putative protein; protein id: At4g34180.1, supported by cDNA:
           8686., supported by cDNA: gi_14335097, supported by
           cDNA: gi_16226602 [Arabidopsis thaliana]
           gi|14335098|gb|AAK59828.1| AT4g34180/F28A23_60
           [Arabidopsis thaliana]
           gi|16226603|gb|AAL16211.1|AF428442_1 AT4g34180/F28A23_60
           [Arabidopsis thaliana] gi|21617901|gb|AAM66951.1|
           unknown [Arabidopsis thaliana]
           gi|21928057|gb|AAM78057.1| AT4g34180/F28A23_60
           [Arabidopsis thaliana]
          Length = 255

 Score =  141 bits (355), Expect = 2e-33
 Identities = 68/96 (70%), Positives = 78/96 (80%), Gaps = 1/96 (1%)
 Frame = +1

Query: 157 PPRREVYDDAGIYDITHRYVPEMPVWNSKEGLGH-FVWLAQSMKNGSWANGSEMKLGVHT 333
           P RREVY+   IYDI+HRY PE+P W S EGLG  F+ LA SMKNGS+AN SEMKL VH+
Sbjct: 29  PIRREVYEGGKIYDISHRYTPEIPAWESSEGLGKTFLRLAASMKNGSFANVSEMKLSVHS 88

Query: 334 GTHVDAPSHFYDNYLDAGFDVDTLDLRVLNGLALLI 441
           GTHVDAP HF+DNY DAGFD D+LDL+VLNG ALL+
Sbjct: 89  GTHVDAPGHFWDNYYDAGFDTDSLDLQVLNGPALLV 124

>ref|NP_175091.1| hypothetical protein; protein id: At1g44542.1 [Arabidopsis
           thaliana] gi|13876507|gb|AAK43483.1|AC084807_8
           hypothetical protein [Arabidopsis thaliana]
          Length = 271

 Score =  134 bits (337), Expect = 3e-31
 Identities = 72/143 (50%), Positives = 98/143 (68%), Gaps = 1/143 (0%)
 Frame = +1

Query: 16  MKNLSLLVAILCAISLHSVAATSSAYPTTPGLDSGDCTLAGGDSLLVPPRREVYD-DAGI 192
           M +L +++  L   S++   A   A+P+ P   S   T    D  + P   EVYD +  I
Sbjct: 1   MYHLLIIITTLSFSSINITFAVDEAFPSIPTTFSV-ATKQHYD--VKPIHHEVYDGERKI 57

Query: 193 YDITHRYVPEMPVWNSKEGLGHFVWLAQSMKNGSWANGSEMKLGVHTGTHVDAPSHFYDN 372
           YDI+H+Y PE+PVW S EGLG+F+ LA SMKNGS AN S+M+L VH+GTHVDAP HF+D+
Sbjct: 58  YDISHQYTPELPVWESSEGLGNFLRLAVSMKNGSDANISKMELSVHSGTHVDAPGHFHDH 117

Query: 373 YLDAGFDVDTLDLRVLNGLALLI 441
           Y ++GFD D+LDL++LNG ALL+
Sbjct: 118 YYESGFDTDSLDLQILNGPALLV 140

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 425,953,774
Number of Sequences: 1393205
Number of extensions: 10168585
Number of successful extensions: 40758
Number of sequences better than 10.0: 188
Number of HSP's better than 10.0 without gapping: 36711
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40509
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 6689237688
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf084f06 BP073579 1 442 2 GNf086e04 BP073716 13 435

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