>KMC003896A_C01 KMC003896A_c01
gagtgtctatatatatcaaagtgttagaaaatgcaacatgaactgatcaagtgatcttag
ctgagatgagattacttagtTTAAGACTTTTTAGTAAATAAACAACATTATGAAAGTGAA
CATAATTTATTCAAACCAAGAAGAACACTTAAACTGACCAAACTAGTTGGACCCTCCCTG
AACCTCCCATCCCCAAGCTGTGCTTATAATATAGCTATTGCTATATATATCCCCCATAAG
CCGCAAAGCCATACCCAAGGACTTACCATCACAATGGAAAATAAAACTAAAGTAATAAAC
AAATAGAGTAGAAAAGTTTCTCAACTTCGACACATGCCAAAAGGACTAGACTCCTTTTTC
AGTTTTCAACACTCATACTGTCCACTGGTCTATGCTTCAGTCTTCTCAATAGCAACTTCT
GTGGTGGCTTCCACAGTGGTTTCAGCTGCTTTGCTCTCCTCAGATGACTTCTCTTCCTCC
ACTGGTGCTGCCTCAGTTGCAGCAGTTTCTTTAGTCTCCTCAACTATTGGTTCCTCTTTC
ACTTCTTCAGGTTTTACCTCTGGTTCCTCTGTTTTCTCTGCCTCAGGCTTGGTTTCTTCT
GTCACTTCTTTAGTCTCAACTTCAGCTGGGGCTTCGGTTTCTGGAGCAGCAGGTGCTTCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003896A_C01 KMC003896A_c01
         (660 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q39967|ALL5_HEVBR Major latex allergen Hev b 5 gi|7488892|pir...    74  2e-12
gb|AAD32205.1|AF134731_1 putative allergen protein [Prunus armen...    74  2e-12
pir||T10699 hypothetical protein - common buckwheat gi|2317676|d...    67  2e-10
emb|CAB85627.1| putative ripening-related protein [Vitis vinifera]     67  2e-10
pir||S51232 gibberellin-responsive ovarian protein G14 - garden ...    64  2e-09

>sp|Q39967|ALL5_HEVBR Major latex allergen Hev b 5 gi|7488892|pir||T10768 latex allergen
           Hev b5 - Para rubber tree gi|1480457|gb|AAC49447.1|
           latex allergen gi|1493836|gb|AAC49448.1| pI 3.5 latex
           allergen
          Length = 151

 Score = 73.6 bits (179), Expect = 2e-12
 Identities = 50/92 (54%), Positives = 55/92 (59%), Gaps = 4/92 (4%)
 Frame = -2

Query: 659 EAPAAPETEAPAEVETKEVTEETKPEAEKT--EEPEVKPEEVKEEPIVEETKETAA-TEA 489
           EAPA PETE   EVE  E TEE  PEA++T  EE   +PE V EE    ETKET     A
Sbjct: 60  EAPA-PETEKAEEVEKIEKTEEPAPEADQTTPEEKPAEPEPVAEEEPKHETKETETEAPA 118

Query: 488 APVEEEKSSEESKAAETTVE-ATTEVAIEKTE 396
           AP E EK +EE K      E ATTEV +EKTE
Sbjct: 119 APAEGEKPAEEEKPITEAAETATTEVPVEKTE 150

>gb|AAD32205.1|AF134731_1 putative allergen protein [Prunus armeniaca]
          Length = 168

 Score = 73.6 bits (179), Expect = 2e-12
 Identities = 49/101 (48%), Positives = 56/101 (54%), Gaps = 13/101 (12%)
 Frame = -2

Query: 659 EAPAAPETEAP--AEVETKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKE-TAATEA 489
           EAPAAPE E P  AEVETKEV EE K    + EEP     E  E+   EE KE TAA   
Sbjct: 67  EAPAAPEAETPVPAEVETKEVAEEPKAAEAEAEEPAATETEKTEKTEAEEPKEVTAAEPV 126

Query: 488 APVEEEKSSEESKAAETTV----------EATTEVAIEKTE 396
           A V EE   E +++AET            EATT+V +EKTE
Sbjct: 127 AAVAEETKEETTESAETPAAPPAEEEKAEEATTDVPVEKTE 167

 Score = 42.0 bits (97), Expect = 0.007
 Identities = 35/88 (39%), Positives = 37/88 (41%), Gaps = 1/88 (1%)
 Frame = -2

Query: 656 APAA-PETEAPAEVETKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAATEAAPV 480
           APAA PE+EA    E KE T   + EAE   E    PE     P   ETKE A       
Sbjct: 37  APAAVPESEAATTEEPKETTP-VEAEAEAEAEAPAAPEAETPVPAEVETKEVA------- 88

Query: 479 EEEKSSEESKAAETTVEATTEVAIEKTE 396
                 EE KAAE   E       EKTE
Sbjct: 89  ------EEPKAAEAEAEEPAATETEKTE 110

>pir||T10699 hypothetical protein - common buckwheat gi|2317676|dbj|BAA21761.1|
           declined protein during seed development [Fagopyrum
           esculentum]
          Length = 159

 Score = 67.4 bits (163), Expect = 2e-10
 Identities = 45/95 (47%), Positives = 53/95 (55%), Gaps = 7/95 (7%)
 Frame = -2

Query: 659 EAPAAPETEAPAEVE-----TKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAAT 495
           EAPAA E EAPA VE     TKEV EE    AE+   PE + EE K E + EE ++    
Sbjct: 66  EAPAAVEEEAPAAVEEAVAETKEVVEEEVKAAEEAPTPEAE-EEKKVEEVEEEKQQVVEA 124

Query: 494 EAAPVEEEKSSEESKAA--ETTVEATTEVAIEKTE 396
           E AP   E  +EE+ A   E   EATTE A+EK E
Sbjct: 125 EEAPAAVEAPAEEAPAVVEEKAEEATTEAAVEKAE 159

 Score = 42.7 bits (99), Expect = 0.004
 Identities = 33/91 (36%), Positives = 41/91 (44%), Gaps = 3/91 (3%)
 Frame = -2

Query: 659 EAPAAPETEAPAEVETKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAATEAAPV 480
           E  A  + E PA  E     EE     E+  E E  P  ++E P   E +  AA E A  
Sbjct: 26  EVAAVEQVETPAVEEAPAAVEEAPAAVEEAVEEEA-PAAIEEAPAAVEEEAPAAVEEAVA 84

Query: 479 E-EEKSSEESKAAE--TTVEATTEVAIEKTE 396
           E +E   EE KAAE   T EA  E  +E+ E
Sbjct: 85  ETKEVVEEEVKAAEEAPTPEAEEEKKVEEVE 115

 Score = 35.8 bits (81), Expect = 0.52
 Identities = 29/87 (33%), Positives = 38/87 (43%), Gaps = 5/87 (5%)
 Frame = -2

Query: 632 APAEVETKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAATEAAPVEEEKSSEES 453
           A  EVE    T +   ++E  +  EV   E  E P VEE    AA E AP   E++ EE 
Sbjct: 2   AAVEVEPNTTTLQENEKSEVAQVEEVAAVEQVETPAVEEA--PAAVEEAPAAVEEAVEEE 59

Query: 452 -----KAAETTVEATTEVAIEKTEA*T 387
                + A   VE     A+E+  A T
Sbjct: 60  APAAIEEAPAAVEEEAPAAVEEAVAET 86

>emb|CAB85627.1| putative ripening-related protein [Vitis vinifera]
          Length = 152

 Score = 67.0 bits (162), Expect = 2e-10
 Identities = 46/98 (46%), Positives = 54/98 (54%), Gaps = 10/98 (10%)
 Frame = -2

Query: 659 EAPAAPETEAPAE------VETKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAA 498
           E  AAPE EA AE      VETKEV EE   E  + E+ E +   V+ E  VE+ KE   
Sbjct: 54  EEAAAPEAEAKAEAPVEVEVETKEVVEEAAVEEAEAEKTEGETPAVEAEAAVEKAKEETT 113

Query: 497 TE--AAPVEE--EKSSEESKAAETTVEATTEVAIEKTE 396
            E  AAP EE   K  EE+K AE   EA+TEV +EK E
Sbjct: 114 EEAAAAPAEEAAAKEEEEAKPAEAAEEASTEVPVEKIE 151

 Score = 45.8 bits (107), Expect = 5e-04
 Identities = 35/89 (39%), Positives = 41/89 (45%), Gaps = 11/89 (12%)
 Frame = -2

Query: 626 AEVETKEVTEETKPEAEKTEE---------PEVKPEEVKEEPIVEETKETAATE--AAPV 480
           A VE   VT    PE EKTEE          EV+      EP+ EETKE A  E  AAP 
Sbjct: 2   ATVEVASVTTAL-PENEKTEELNKVEEGAVKEVEAPPAAPEPVTEETKEEAPVEEAAAPE 60

Query: 479 EEEKSSEESKAAETTVEATTEVAIEKTEA 393
            E K+    +    T E   E A+E+ EA
Sbjct: 61  AEAKAEAPVEVEVETKEVVEEAAVEEAEA 89

>pir||S51232 gibberellin-responsive ovarian protein G14 - garden pea  (fragment)
           gi|633605|emb|CAA87711.1| ovary protein induced by
           treatment with gibberellic acid [Pisum sativum]
          Length = 204

 Score = 63.9 bits (154), Expect = 2e-09
 Identities = 38/75 (50%), Positives = 45/75 (59%)
 Frame = -2

Query: 641 ETEAPAEVETKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAATEAAPVEEEKSS 462
           ETE P E ET EV EE KPE E     +V  EEVKEE   EE KET  TE+A      + 
Sbjct: 105 ETEVPVEAETTEVAEEAKPEVENLATEKV--EEVKEEATTEEAKETTETESAVA--SAAV 160

Query: 461 EESKAAETTVEATTE 417
           EE+KAAET V+ + +
Sbjct: 161 EENKAAETAVKMSPQ 175

 Score = 39.7 bits (91), Expect = 0.036
 Identities = 37/106 (34%), Positives = 46/106 (42%), Gaps = 27/106 (25%)
 Frame = -2

Query: 632 APAEVETKEV----------TEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAATE--- 492
           A  EVET++V           +ET P +E      V  E+ KEE + EE    AA E   
Sbjct: 45  ATVEVETQQVVPNETVEVTKVDETTPASEVLVSEPVTTEQPKEEAVAEEAVAEAAVEKEA 104

Query: 491 --AAPVEEEKS--SEES----------KAAETTVEATTEVAIEKTE 396
               PVE E +  +EE+          K  E   EATTE A E TE
Sbjct: 105 ETEVPVEAETTEVAEEAKPEVENLATEKVEEVKEEATTEEAKETTE 150

 Score = 39.7 bits (91), Expect = 0.036
 Identities = 38/90 (42%), Positives = 45/90 (49%), Gaps = 8/90 (8%)
 Frame = -2

Query: 641 ETEAPAEVETKEVT--EETKPEAEKTEEPEVKPEEVKEEPIVEETKETAATEAAPVEEEK 468
           ET+     ET EVT  +ET P +E      V  E+ KEE + EE    A  EAA VE+E 
Sbjct: 50  ETQQVVPNETVEVTKVDETTPASEVLVSEPVTTEQPKEEAVAEE----AVAEAA-VEKEA 104

Query: 467 SSEESKAAETT---VEATTEV---AIEKTE 396
            +E    AETT    EA  EV   A EK E
Sbjct: 105 ETEVPVEAETTEVAEEAKPEVENLATEKVE 134

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 527,508,358
Number of Sequences: 1393205
Number of extensions: 11532120
Number of successful extensions: 82349
Number of sequences better than 10.0: 2937
Number of HSP's better than 10.0 without gapping: 55720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74570
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28289785200
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB004e06_f BP034191 1 470 2 SPD047g09_f BP047774 99 413 3 GNf084d10 BP073561 112 556 4 SPD025h12_f BP046018 113 640 5 MR048e10_f BP079723 113 235 6 MF050d07_f BP030929 119 480 7 MF023f04_f BP029491 119 661

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