KMC003856A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC003856A_C01 KMC003856A_c01
aaaaaagtaacatttttctatgatatgagcttcggaaaattaaacagcacaaatagatta
tagttagtgagcaagtaaccAGTTAAGTAAGTGATTCACATTATCAAAATATTTTATAAA
AGAAAGATTACAGCAAGAGATTATCATACTCATACAAGTGGAAATTCTAACTTAGGCTGG
AAGTTTGTAATGATTAATAACATCTATAATTCATCGCGTATATTTCCCTTCCGGCCAGGG
TCCATCTTGCAGATATTTTTCAAGATAATTAACAGCTAGTGATGCGTGAAGTGCGGCCCC
ATAAGGAAGTCCTTCTTCATTGATCACGAGATAGGGTGAGTGCACAAAGTGAGCCCGGTG
ATCATTTGAGGGCCTTCTGCATTCCAAAAAAAAAGAAGTAGCCAGGTATCACCTTTAGAT
AGAATGAAAAGTCTTCAGAAGCCGTGACCGGTGGCTTGTCAAAGTGGACTTTATTGGCGC
CAAGC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003856A_C01 KMC003856A_c01
         (485 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAA73905.1| JR3 protein [Arabidopsis thaliana]                     45  8e-10
ref|NP_175587.1| IAA-Ala hydrolase (IAR3); protein id: At1g51760...    45  8e-10
gb|AAL59907.1| IAA-amino acid hydrolase [Arabidopsis thaliana]         44  1e-08
ref|NP_200477.1| IAA-amino acid hydrolase (gb|AAC04866.1); prote...    44  1e-08
gb|AAC49016.1| ILL2                                                    44  1e-08

>emb|CAA73905.1| JR3 protein [Arabidopsis thaliana]
          Length = 444

 Score = 45.1 bits (105), Expect(2) = 8e-10
 Identities = 19/27 (70%), Positives = 22/27 (81%)
 Frame = -3

Query: 342 HSPYLVINEEGLPYGAALHASLAVNYL 262
           HSPY  +NEE LPYGA+LHAS+A  YL
Sbjct: 401 HSPYFEVNEELLPYGASLHASMATRYL 427

 Score = 38.9 bits (89), Expect(2) = 8e-10
 Identities = 16/30 (53%), Positives = 21/30 (69%)
 Frame = -2

Query: 466 HFDKPPVTASEDFSFYLKVIPGYFFFFGMQ 377
           + +  P+  SEDFSFY + IPG+F F GMQ
Sbjct: 361 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQ 390

>ref|NP_175587.1| IAA-Ala hydrolase (IAR3); protein id: At1g51760.1, supported by
           cDNA: gi_14030706 [Arabidopsis thaliana]
           gi|25319488|pir||F96556 IAA-Ala hydrolase (IAR3)
           [imported] - Arabidopsis thaliana
           gi|3421384|gb|AAC32192.1| IAA-Ala hydrolase; IAA-amino
           acid hydrolase [Arabidopsis thaliana]
           gi|12321681|gb|AAG50883.1|AC025294_21 IAA-Ala hydrolase
           (IAR3) [Arabidopsis thaliana]
           gi|14030707|gb|AAK53028.1|AF375444_1 At1g51760/F19C24_4
           [Arabidopsis thaliana] gi|23506081|gb|AAN28900.1|
           At1g51760/F19C24_4 [Arabidopsis thaliana]
          Length = 440

 Score = 45.1 bits (105), Expect(2) = 8e-10
 Identities = 19/27 (70%), Positives = 22/27 (81%)
 Frame = -3

Query: 342 HSPYLVINEEGLPYGAALHASLAVNYL 262
           HSPY  +NEE LPYGA+LHAS+A  YL
Sbjct: 397 HSPYFEVNEELLPYGASLHASMATRYL 423

 Score = 38.9 bits (89), Expect(2) = 8e-10
 Identities = 16/30 (53%), Positives = 21/30 (69%)
 Frame = -2

Query: 466 HFDKPPVTASEDFSFYLKVIPGYFFFFGMQ 377
           + +  P+  SEDFSFY + IPG+F F GMQ
Sbjct: 357 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQ 386

>gb|AAL59907.1| IAA-amino acid hydrolase [Arabidopsis thaliana]
          Length = 439

 Score = 44.3 bits (103), Expect(2) = 1e-08
 Identities = 20/34 (58%), Positives = 25/34 (72%)
 Frame = -3

Query: 342 HSPYLVINEEGLPYGAALHASLAVNYLEKYLQDG 241
           HSP   INE+ LPYGAA+HAS+AV YL++    G
Sbjct: 397 HSPLYRINEDVLPYGAAIHASMAVQYLKEKASKG 430

 Score = 35.4 bits (80), Expect(2) = 1e-08
 Identities = 14/25 (56%), Positives = 18/25 (72%)
 Frame = -2

Query: 451 PVTASEDFSFYLKVIPGYFFFFGMQ 377
           PV  SEDFS++ + IPG+F   GMQ
Sbjct: 363 PVMGSEDFSYFAETIPGHFSLLGMQ 387

>ref|NP_200477.1| IAA-amino acid hydrolase (gb|AAC04866.1); protein id: At5g56660.1,
           supported by cDNA: gi_18175666, supported by cDNA:
           gi_902790 [Arabidopsis thaliana]
           gi|21264464|sp|P54970|ILL2_ARATH IAA-amino acid
           hydrolase homolog 2 precursor gi|2921830|gb|AAC04866.1|
           IAA-amino acid hydrolase [Arabidopsis thaliana]
           gi|10176770|dbj|BAB09884.1| IAA-amino acid hydrolase
           [Arabidopsis thaliana]
          Length = 439

 Score = 44.3 bits (103), Expect(2) = 1e-08
 Identities = 20/34 (58%), Positives = 25/34 (72%)
 Frame = -3

Query: 342 HSPYLVINEEGLPYGAALHASLAVNYLEKYLQDG 241
           HSP   INE+ LPYGAA+HAS+AV YL++    G
Sbjct: 397 HSPLYRINEDVLPYGAAIHASMAVQYLKEKASKG 430

 Score = 35.4 bits (80), Expect(2) = 1e-08
 Identities = 14/25 (56%), Positives = 18/25 (72%)
 Frame = -2

Query: 451 PVTASEDFSFYLKVIPGYFFFFGMQ 377
           PV  SEDFS++ + IPG+F   GMQ
Sbjct: 363 PVMGSEDFSYFAETIPGHFSLLGMQ 387

>gb|AAC49016.1| ILL2
          Length = 439

 Score = 44.3 bits (103), Expect(2) = 1e-08
 Identities = 20/34 (58%), Positives = 25/34 (72%)
 Frame = -3

Query: 342 HSPYLVINEEGLPYGAALHASLAVNYLEKYLQDG 241
           HSP   INE+ LPYGAA+HAS+AV YL++    G
Sbjct: 397 HSPLYRINEDVLPYGAAIHASMAVQYLKEKASKG 430

 Score = 35.4 bits (80), Expect(2) = 1e-08
 Identities = 14/25 (56%), Positives = 18/25 (72%)
 Frame = -2

Query: 451 PVTASEDFSFYLKVIPGYFFFFGMQ 377
           PV  SEDFS++ + IPG+F   GMQ
Sbjct: 363 PVMGSEDFSYFAETIPGHFSLLGMQ 387

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 390,031,779
Number of Sequences: 1393205
Number of extensions: 7751523
Number of successful extensions: 14657
Number of sequences better than 10.0: 82
Number of HSP's better than 10.0 without gapping: 14371
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14657
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13980339936
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MR018b10_f BP077338 1 481
2 GNf080a08 BP073243 104 486




Lotus japonicus
Kazusa DNA Research Institute