KMC003833A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC003833A_C01 KMC003833A_c01
aacgcagaagaaacattactctgaattgtATTAGAGCATGTGTAAGTACTTCAAACTTCA
AATATGCAAATACAAAGTATCCAGCAAATGCTTATTTACACATCCTACTCTGGTCAAACT
TACTGCTAATGGCCTCCCAGAAGGCAGGAATAGAGATGTCATCAGGTACTTCCTTAAATG
ATGGGAGATAGTTTACATCAACAAGTTACATGATCATGAGTACCTTCCTGAATTACAACA
TCAAAACCAAAGCACTGGGAGATGGAGCCTTCTCCTAAGCCAATTTGCAGCATCTGTAAC
CAGTTTTATATCAATAGACTCATCATTTGTCAGACCAGGAGTACTGGCTCCAGAATCCTT
GACACTGTCAGCAGTCGGTAGAGATTTCAAGCTATCAAATAACAGAGGTTGGAGGTCATC
ACCATTAGATAATTTCATCAAAATATCAGCATTAGGTATAGACTTCTTCA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003833A_C01 KMC003833A_c01
         (470 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_181926.2| hypothetical protein; protein id: At2g43980.1, ...    60  4e-24
ref|NP_035740.1| tumor necrosis factor receptor superfamily, mem...    32  2.6
ref|NP_107044.1| hypothetical protein [Mesorhizobium loti] gi|14...    32  2.6
pir||I48854 gene murine tumour necrosis factor receptor 2 protei...    32  2.6
sp|P25119|TR1B_MOUSE Tumor necrosis factor receptor superfamily ...    32  2.6

>ref|NP_181926.2| hypothetical protein; protein id: At2g43980.1, supported by cDNA:
           gi_17979419, supported by cDNA: gi_20465988 [Arabidopsis
           thaliana] gi|17979420|gb|AAL49852.1| unknown protein
           [Arabidopsis thaliana] gi|20465989|gb|AAM20216.1|
           unknown protein [Arabidopsis thaliana]
          Length = 488

 Score = 59.7 bits (143), Expect(3) = 4e-24
 Identities = 34/75 (45%), Positives = 47/75 (62%)
 Frame = -3

Query: 468 KKSIPNADILMKLSNGDDLQPLLFDSLKSLPTADSVKDSGASTPGLTNDESIDIKLVTDA 289
           KKSIP++  L K +  + L+P+LFDSLKSLP  DS   +  S         ID++LVT+A
Sbjct: 373 KKSIPSSSSLRKSAEENGLKPILFDSLKSLP-VDSANQNPVS--------EIDLELVTEA 423

Query: 288 ANWLRRRLHLPVLWF 244
           A WLR++L L +  F
Sbjct: 424 ATWLRKKLDLTIFGF 438

 Score = 59.7 bits (143), Expect(3) = 4e-24
 Identities = 24/32 (75%), Positives = 31/32 (96%)
 Frame = -2

Query: 205 LVDVNYLPSFKEVPDDISIPAFWEAISSKFDQ 110
           +VD+NYLPSFKEVPD+I++PAFWEAI ++FDQ
Sbjct: 451 IVDLNYLPSFKEVPDNIAVPAFWEAIRNRFDQ 482

 Score = 32.7 bits (73), Expect(3) = 4e-24
 Identities = 14/15 (93%), Positives = 14/15 (93%)
 Frame = -1

Query: 251 FGFDVVIQEGTHDHV 207
           FGFDVVIQEGT DHV
Sbjct: 436 FGFDVVIQEGTGDHV 450

>ref|NP_035740.1| tumor necrosis factor receptor superfamily, member 1b; p75 TNFR;
           TNF receptor beta chain [Mus musculus]
           gi|3219683|emb|CAA74969.1| p80 TNF-alpha receptor [Mus
           musculus]
          Length = 482

 Score = 32.3 bits (72), Expect = 2.6
 Identities = 18/45 (40%), Positives = 24/45 (53%)
 Frame = +2

Query: 296 VTSFISIDSSFVRPGVLAPESLTLSAVGRDFKLSNNRGWRSSPLD 430
           + S ++I  +     V APES TLSA+ R   +S     RS PLD
Sbjct: 193 ICSILAIPGNASTDAVCAPESPTLSAIPRTLYVSQPEPTRSQPLD 237

>ref|NP_107044.1| hypothetical protein [Mesorhizobium loti]
           gi|14026232|dbj|BAB52830.1| hypothetical protein
           [Mesorhizobium loti]
          Length = 1562

 Score = 32.3 bits (72), Expect = 2.6
 Identities = 23/79 (29%), Positives = 38/79 (47%), Gaps = 5/79 (6%)
 Frame = +2

Query: 188 IVYINKLHDHEYLP-----ELQHQNQSTGRWSLLLSQFAASVTSFISIDSSFVRPGVLAP 352
           +V ++ ++D  +LP      L+    S  RW  L+  FAA  TS +  D     PG+   
Sbjct: 1   MVGVDPVNDEFFLPPPGLTSLEPLEPSPTRW--LIKNFAAPRTSELQFD-----PGLGLD 53

Query: 353 ESLTLSAVGRDFKLSNNRG 409
           E + +   G  +++SN RG
Sbjct: 54  EDMLVEIAGSQYRISNPRG 72

>pir||I48854 gene murine tumour necrosis factor receptor 2 protein - mouse
           (fragment) gi|433831|emb|CAA53981.1| murine tumour
           necrosis factor receptor 2 [Mus musculus]
          Length = 459

 Score = 32.3 bits (72), Expect = 2.6
 Identities = 18/45 (40%), Positives = 24/45 (53%)
 Frame = +2

Query: 296 VTSFISIDSSFVRPGVLAPESLTLSAVGRDFKLSNNRGWRSSPLD 430
           + S ++I  +     V APES TLSA+ R   +S     RS PLD
Sbjct: 171 ICSILAIPGNASTDAVCAPESPTLSAIPRTLYVSQPEPTRSQPLD 215

>sp|P25119|TR1B_MOUSE Tumor necrosis factor receptor superfamily member 1B precursor
           (Tumor necrosis factor receptor 2) (TNF-R2) (p75)
           gi|91863|pir||B38634 tumor necrosis factor receptor type
           2 precursor - mouse gi|199828|gb|AAA39752.1| murine
           tumor necrosis factor receptor 2
           gi|202095|gb|AAA40463.1| tumor necrosis factor receptor
           1 gi|12836165|dbj|BAB23533.1| unnamed protein product
           [Mus musculus] gi|12836334|dbj|BAB23610.1| unnamed
           protein product [Mus musculus]
          Length = 474

 Score = 32.3 bits (72), Expect = 2.6
 Identities = 18/45 (40%), Positives = 24/45 (53%)
 Frame = +2

Query: 296 VTSFISIDSSFVRPGVLAPESLTLSAVGRDFKLSNNRGWRSSPLD 430
           + S ++I  +     V APES TLSA+ R   +S     RS PLD
Sbjct: 186 ICSILAIPGNASTDAVCAPESPTLSAIPRTLYVSQPEPTRSQPLD 230

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 378,643,688
Number of Sequences: 1393205
Number of extensions: 7682961
Number of successful extensions: 18076
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 17719
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18071
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 12524054526
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GNf078c01 BP073112 1 404
2 MPD036d07_f AV772457 30 470




Lotus japonicus
Kazusa DNA Research Institute