>KMC003800A_C01 KMC003800A_c01
aacagacattttctcaactgtactatatgcattacaagactggatacaaataggtaaact
tgaataaatacaAGTTCAGTTTGTTGCTCAAGTCTTATAATATAACCTAACCATATTTCA
CAAAACCAGGTAGTTGAAGGAGGAAAGCCAGCTTATATGGATGTAGTTGTGAATAAACAA
ACATCCCATAAGATCAATCATAAACCCTTAGAACCGGGAAGATCAATCCAGTGCAACGAG
ATTTCCACTTCACCACATTCGACATGTCGCAATCTGAGGATCATGTCTTGGATAATTTGG
CCGCTGTTATAATAGATACAGCTCTCGTCCGCCAAGCAATTCTCCGTGGCGTGGTTGTAT
TCTCTTGATCACGGTGCCGAGGGGAAGGCCAGTTACGTTCATCTTCAAGGCTTCTATATA
GGGAAAGATTTCAAATTCCGCATCTCCCATTTAGTCATCTTAGCTAAAAGTGTCATGGTC
ATACACAGTCAGCCAAACGGAGTGATGAGGATCTATGACAGAAAGGGTAAGGTCTTCATT
CCACTCAGGATTAACATCCTTCTTAATCACACGAGTCTTAAGTTTCTGATTGTACATCTT
AACAACAACATAAGGGTCACTGCTACGAACATCACGAACAGCAAGGTTGACGCCACGCTT
AACACGAACCCTGAGAAGGCCTAGCAGGTTCTCCATCAGAGACTTTGGTGTTGAATCTTC
CATTTCTTTCTTTCTTTCTTccctctttatattatgattataataataacataacctgta
ataaaatcattaaatgtgttgatgaggggggcccgaccca

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003800A_C01 KMC003800A_c01
         (820 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

dbj|BAB02719.1| GTPase activating protein-like [Arabidopsis thal...   176  4e-59
ref|NP_188425.1| unknown protein; protein id: At3g17980.1 [Arabi...   173  3e-58
pir||E96525 protein T1N15.21 [imported] - Arabidopsis thaliana g...   169  6e-56
ref|NP_175292.1| unknown protein; protein id: At1g48590.1 [Arabi...   169  6e-56
ref|NP_177499.1| hypothetical protein; protein id: At1g73580.1 [...   161  2e-51

>dbj|BAB02719.1| GTPase activating protein-like [Arabidopsis thaliana]
          Length = 177

 Score =  176 bits (446), Expect(2) = 4e-59
 Identities = 87/117 (74%), Positives = 100/117 (85%)
 Frame = -2

Query: 702 SLMENLLGLLRVRVKRGVNLAVRDVRSSDPYVVVKMYNQKLKTRVIKKDVNPEWNEDLTL 523
           SLM++LLGLLR+R+KRGVNLAVRD+ SSDPYVVVKM  QKLKTRVI KDVNPEWNEDLTL
Sbjct: 11  SLMDDLLGLLRIRIKRGVNLAVRDISSSDPYVVVKMGKQKLKTRVINKDVNPEWNEDLTL 70

Query: 522 SVIDPHHSVWLTVYDHDTFS*DD*MGDAEFEIFPYIEALKMNVTGLPLGTVIKRIQP 352
           SV D + +V LTVYDHD FS DD MGDAEFEI PYIEAL+M + GLP GT++  ++P
Sbjct: 71  SVTDSNLTVLLTVYDHDMFSKDDKMGDAEFEIKPYIEALRMQLDGLPSGTIVTTVKP 127

 Score = 75.1 bits (183), Expect(2) = 4e-59
 Identities = 32/47 (68%), Positives = 40/47 (85%)
 Frame = -3

Query: 341 NCLADESCIYYNSGQIIQDMILRLRHVECGEVEISLHWIDLPGSKGL 201
           NCLA+ES + +  G+++QD++LRLRHVECGEVE  L WIDLPGSKGL
Sbjct: 131 NCLAEESRVTWVDGKLVQDLVLRLRHVECGEVEAQLQWIDLPGSKGL 177

>ref|NP_188425.1| unknown protein; protein id: At3g17980.1 [Arabidopsis thaliana]
          Length = 165

 Score =  173 bits (438), Expect(2) = 3e-58
 Identities = 85/115 (73%), Positives = 98/115 (84%)
 Frame = -2

Query: 696 MENLLGLLRVRVKRGVNLAVRDVRSSDPYVVVKMYNQKLKTRVIKKDVNPEWNEDLTLSV 517
           M++LLGLLR+R+KRGVNLAVRD+ SSDPYVVVKM  QKLKTRVI KDVNPEWNEDLTLSV
Sbjct: 1   MDDLLGLLRIRIKRGVNLAVRDISSSDPYVVVKMGKQKLKTRVINKDVNPEWNEDLTLSV 60

Query: 516 IDPHHSVWLTVYDHDTFS*DD*MGDAEFEIFPYIEALKMNVTGLPLGTVIKRIQP 352
            D + +V LTVYDHD FS DD MGDAEFEI PYIEAL+M + GLP GT++  ++P
Sbjct: 61  TDSNLTVLLTVYDHDMFSKDDKMGDAEFEIKPYIEALRMQLDGLPSGTIVTTVKP 115

 Score = 75.1 bits (183), Expect(2) = 3e-58
 Identities = 32/47 (68%), Positives = 40/47 (85%)
 Frame = -3

Query: 341 NCLADESCIYYNSGQIIQDMILRLRHVECGEVEISLHWIDLPGSKGL 201
           NCLA+ES + +  G+++QD++LRLRHVECGEVE  L WIDLPGSKGL
Sbjct: 119 NCLAEESRVTWVDGKLVQDLVLRLRHVECGEVEAQLQWIDLPGSKGL 165

>pir||E96525 protein T1N15.21 [imported] - Arabidopsis thaliana
           gi|8778695|gb|AAF79703.1|AC020889_11 T1N15.21
           [Arabidopsis thaliana]
          Length = 200

 Score =  169 bits (428), Expect(2) = 6e-56
 Identities = 85/133 (63%), Positives = 104/133 (77%)
 Frame = -2

Query: 732 KKEMEDSTPKSLMENLLGLLRVRVKRGVNLAVRDVRSSDPYVVVKMYNQKLKTRVIKKDV 553
           +K  +     SLM++LLGLLR+R+KRGVNLAVRD+ SSDPYVVVKM  QKLKTRVI K+V
Sbjct: 25  EKHKDRRMKPSLMDSLLGLLRIRIKRGVNLAVRDLNSSDPYVVVKMAKQKLKTRVIYKNV 84

Query: 552 NPEWNEDLTLSVIDPHHSVWLTVYDHDTFS*DD*MGDAEFEIFPYIEALKMNVTGLPLGT 373
           NPEWNEDLTLSV DP+ +V LTVYD+DTF+ DD MGDAEF I P++ ALKM++  LP GT
Sbjct: 85  NPEWNEDLTLSVSDPNLTVLLTVYDYDTFTKDDKMGDAEFGIKPFVNALKMHLHDLPSGT 144

Query: 372 VIKRIQPRHGELL 334
           ++  +QP     L
Sbjct: 145 IVTTVQPSRDNCL 157

 Score = 71.2 bits (173), Expect(2) = 6e-56
 Identities = 31/48 (64%), Positives = 41/48 (84%)
 Frame = -3

Query: 344 ENCLADESCIYYNSGQIIQDMILRLRHVECGEVEISLHWIDLPGSKGL 201
           +NCLA+ES + ++ G+++QD++LRLRHVECGEVE  L WIDLPG KGL
Sbjct: 154 DNCLAEESRVIWSDGKLVQDIVLRLRHVECGEVEAQLQWIDLPG-KGL 200

>ref|NP_175292.1| unknown protein; protein id: At1g48590.1 [Arabidopsis thaliana]
          Length = 185

 Score =  169 bits (428), Expect(2) = 6e-56
 Identities = 85/133 (63%), Positives = 104/133 (77%)
 Frame = -2

Query: 732 KKEMEDSTPKSLMENLLGLLRVRVKRGVNLAVRDVRSSDPYVVVKMYNQKLKTRVIKKDV 553
           +K  +     SLM++LLGLLR+R+KRGVNLAVRD+ SSDPYVVVKM  QKLKTRVI K+V
Sbjct: 10  EKHKDRRMKPSLMDSLLGLLRIRIKRGVNLAVRDLNSSDPYVVVKMAKQKLKTRVIYKNV 69

Query: 552 NPEWNEDLTLSVIDPHHSVWLTVYDHDTFS*DD*MGDAEFEIFPYIEALKMNVTGLPLGT 373
           NPEWNEDLTLSV DP+ +V LTVYD+DTF+ DD MGDAEF I P++ ALKM++  LP GT
Sbjct: 70  NPEWNEDLTLSVSDPNLTVLLTVYDYDTFTKDDKMGDAEFGIKPFVNALKMHLHDLPSGT 129

Query: 372 VIKRIQPRHGELL 334
           ++  +QP     L
Sbjct: 130 IVTTVQPSRDNCL 142

 Score = 71.2 bits (173), Expect(2) = 6e-56
 Identities = 31/48 (64%), Positives = 41/48 (84%)
 Frame = -3

Query: 344 ENCLADESCIYYNSGQIIQDMILRLRHVECGEVEISLHWIDLPGSKGL 201
           +NCLA+ES + ++ G+++QD++LRLRHVECGEVE  L WIDLPG KGL
Sbjct: 139 DNCLAEESRVIWSDGKLVQDIVLRLRHVECGEVEAQLQWIDLPG-KGL 185

>ref|NP_177499.1| hypothetical protein; protein id: At1g73580.1 [Arabidopsis
           thaliana] gi|25406278|pir||F96762 hypothetical protein
           F6D5.3 [imported] - Arabidopsis thaliana
           gi|12323694|gb|AAG51808.1|AC079676_3 hypothetical
           protein; 22552-21875 [Arabidopsis thaliana]
          Length = 168

 Score =  161 bits (407), Expect(2) = 2e-51
 Identities = 79/123 (64%), Positives = 99/123 (80%)
 Frame = -2

Query: 702 SLMENLLGLLRVRVKRGVNLAVRDVRSSDPYVVVKMYNQKLKTRVIKKDVNPEWNEDLTL 523
           SLM+NLLG+LRVRV+RGVNLAVRDV SSDPYVV+K+  QKLKT+V+K++VNP+W EDL+ 
Sbjct: 2   SLMDNLLGILRVRVQRGVNLAVRDVSSSDPYVVLKLGRQKLKTKVVKQNVNPQWQEDLSF 61

Query: 522 SVIDPHHSVWLTVYDHDTFS*DD*MGDAEFEIFPYIEALKMNVTGLPLGTVIKRIQPRHG 343
           +V DP+  + L VYDHD FS DD MGDAE ++ PYIEAL+M ++GLP GT+I  I P  G
Sbjct: 62  TVTDPNLPLTLIVYDHDFFSKDDKMGDAEIDLKPYIEALRMELSGLPDGTIISTIGPSRG 121

Query: 342 ELL 334
             L
Sbjct: 122 NCL 124

 Score = 64.3 bits (155), Expect(2) = 2e-51
 Identities = 31/47 (65%), Positives = 38/47 (79%)
 Frame = -3

Query: 341 NCLADESCIYYNSGQIIQDMILRLRHVECGEVEISLHWIDLPGSKGL 201
           NCLA+ES I + + +I+Q + LRLR+VE GEVEI L WIDLPGSKGL
Sbjct: 122 NCLAEESYIRWINDRIVQHICLRLRNVERGEVEIELQWIDLPGSKGL 168

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 689,598,440
Number of Sequences: 1393205
Number of extensions: 14579666
Number of successful extensions: 41079
Number of sequences better than 10.0: 551
Number of HSP's better than 10.0 without gapping: 38790
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40993
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 42296827742
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf074d02 BP072833 1 495 2 MPD055f11_f AV773704 307 828

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