Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003770A_C02 KMC003770A_c02
(569 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB92783.1| putative branched chain alpha-keto acid dehydrog... 202 2e-51
gb|AAF35280.1|AF145451_1 branched chain alpha-keto acid dehydrog... 199 3e-50
ref|NP_187341.1| branched chain alpha-keto acid dehydrogenase E2... 199 3e-50
pir||T52136 dihydrolipoamide S-acetyltransferase (EC 2.3.1.12) c... 195 4e-49
pir||A32422 dihydrolipoamide S-(2-methylpropanoyl)transferase (E... 135 5e-31
>dbj|BAB92783.1| putative branched chain alpha-keto acid dehydrogenase E2 subunit
[Oryza sativa (japonica cultivar-group)]
Length = 503
Score = 202 bits (514), Expect = 2e-51
Identities = 97/125 (77%), Positives = 114/125 (90%)
Frame = -2
Query: 568 NIKNVQSLSILEITKELARLQQLASDNKLSSEDICGGTITLSNIGAIGGKFGSPLLNLPE 389
NIKNVQSLSILEITKEL+RL ++AS N+LS+EDI GGTITLSNIGAIGGKFGSPLLNLPE
Sbjct: 379 NIKNVQSLSILEITKELSRLHEMASHNRLSTEDIAGGTITLSNIGAIGGKFGSPLLNLPE 438
Query: 388 VSIIAIGRIQKVPQFADDGSVYPASLMTVNIGADHRVLDGATVARFCNEWKQLIENPELL 209
V+IIA+GRIQK+P+F DD +VYP+S++ V +GADHRV+DGATVARFCNEWK L+E PE L
Sbjct: 439 VAIIALGRIQKLPRFDDDENVYPSSIINVTVGADHRVVDGATVARFCNEWKSLVEKPERL 498
Query: 208 TLHLR 194
LH+R
Sbjct: 499 LLHMR 503
>gb|AAF35280.1|AF145451_1 branched chain alpha-keto acid dehydrogenase E2 subunit
[Arabidopsis thaliana]
Length = 483
Score = 199 bits (505), Expect = 3e-50
Identities = 96/125 (76%), Positives = 114/125 (90%)
Frame = -2
Query: 568 NIKNVQSLSILEITKELARLQQLASDNKLSSEDICGGTITLSNIGAIGGKFGSPLLNLPE 389
NIKNVQSLS+LEITKEL+RLQ LA++NKL+ ED+ GGTITLSNIGAIGGKFGSPLLNLPE
Sbjct: 359 NIKNVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPE 418
Query: 388 VSIIAIGRIQKVPQFADDGSVYPASLMTVNIGADHRVLDGATVARFCNEWKQLIENPELL 209
V+IIA+GRI+KVP+F+ +G+VYPAS+M VNI ADHRVLDGATVARFC +WK+ +E PELL
Sbjct: 419 VAIIALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELL 478
Query: 208 TLHLR 194
L +R
Sbjct: 479 MLQMR 483
>ref|NP_187341.1| branched chain alpha-keto acid dehydrogenase E2 subunit; protein
id: At3g06850.1, supported by cDNA: 25128., supported by
cDNA: gi_3138971, supported by cDNA: gi_7021283
[Arabidopsis thaliana] gi|7549628|gb|AAF63813.1|
branched chain alpha-keto acid dehydrogenase E2 subunit
[Arabidopsis thaliana] gi|21554337|gb|AAM63444.1|
branched chain alpha-keto acid dehydrogenase E2 subunit
[Arabidopsis thaliana]
Length = 483
Score = 199 bits (505), Expect = 3e-50
Identities = 96/125 (76%), Positives = 114/125 (90%)
Frame = -2
Query: 568 NIKNVQSLSILEITKELARLQQLASDNKLSSEDICGGTITLSNIGAIGGKFGSPLLNLPE 389
NIKNVQSLS+LEITKEL+RLQ LA++NKL+ ED+ GGTITLSNIGAIGGKFGSPLLNLPE
Sbjct: 359 NIKNVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPE 418
Query: 388 VSIIAIGRIQKVPQFADDGSVYPASLMTVNIGADHRVLDGATVARFCNEWKQLIENPELL 209
V+IIA+GRI+KVP+F+ +G+VYPAS+M VNI ADHRVLDGATVARFC +WK+ +E PELL
Sbjct: 419 VAIIALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELL 478
Query: 208 TLHLR 194
L +R
Sbjct: 479 MLQMR 483
>pir||T52136 dihydrolipoamide S-acetyltransferase (EC 2.3.1.12) chain E2
[imported] - Arabidopsis thaliana
gi|3138972|gb|AAC16694.1| dihydrolipoylacyltransferase
subunit of the branched-chain alpha-keto acid
dehydrogenase complex [Arabidopsis thaliana]
Length = 483
Score = 195 bits (495), Expect = 4e-49
Identities = 95/125 (76%), Positives = 113/125 (90%)
Frame = -2
Query: 568 NIKNVQSLSILEITKELARLQQLASDNKLSSEDICGGTITLSNIGAIGGKFGSPLLNLPE 389
NIKNVQSLS+LEITKEL+RLQ LA++NKL+ ED+ GGTITLSNIGAIGGKFGS LLNLPE
Sbjct: 359 NIKNVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSLLLNLPE 418
Query: 388 VSIIAIGRIQKVPQFADDGSVYPASLMTVNIGADHRVLDGATVARFCNEWKQLIENPELL 209
V+IIA+GRI+KVP+F+ +G+VYPAS+M VNI ADHRVLDGATVARFC +WK+ +E PELL
Sbjct: 419 VAIIALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELL 478
Query: 208 TLHLR 194
L +R
Sbjct: 479 MLQMR 483
>pir||A32422 dihydrolipoamide S-(2-methylpropanoyl)transferase (EC 2.3.1.-)
precursor - human
Length = 482
Score = 135 bits (339), Expect = 5e-31
Identities = 66/125 (52%), Positives = 88/125 (69%)
Frame = -2
Query: 568 NIKNVQSLSILEITKELARLQQLASDNKLSSEDICGGTITLSNIGAIGGKFGSPLLNLPE 389
N+KNVQ SI +I EL RLQ+L S +LS+ D+ GGT TLSNIG+IGG F P++ PE
Sbjct: 358 NVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPE 417
Query: 388 VSIIAIGRIQKVPQFADDGSVYPASLMTVNIGADHRVLDGATVARFCNEWKQLIENPELL 209
V+I A+G I+ +P+F G VY A +M V+ ADHRV+DGAT++RF N WK +ENP +
Sbjct: 418 VAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFM 477
Query: 208 TLHLR 194
L L+
Sbjct: 478 LLDLK 482
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 475,146,783
Number of Sequences: 1393205
Number of extensions: 9927182
Number of successful extensions: 28123
Number of sequences better than 10.0: 432
Number of HSP's better than 10.0 without gapping: 26909
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27815
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20956655091
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
