Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003741A_C01 KMC003741A_c01
(451 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAD66064.1| carbonic anhydrase [Lotus japonicus] 154 2e-37
pir||T09570 carbonate dehydratase (EC 4.2.1.1) - alfalfa gi|1938... 130 5e-30
emb|CAB43571.1| carbonic anhydrase [Glycine max] 125 2e-28
gb|AAD29049.1|AF132854_1 carbonic anhydrase isoform 1 [Gossypium... 91 2e-18
gb|AAM22683.1|AF482951_1 carbonic anhydrase [Gossypium hirsutum] 91 2e-18
>emb|CAD66064.1| carbonic anhydrase [Lotus japonicus]
Length = 263
Score = 154 bits (389), Expect = 2e-37
Identities = 75/92 (81%), Positives = 81/92 (87%)
Frame = +2
Query: 176 VQGCHPTPETAEQRIMDGFTYFKIHNFDKNPDLVQGNWLRATAPRFLSICCGSDSRVSPT 355
+QGCHPTPETAEQRI+DGFTYFKIHNFDKNPDL + + +P+FL C SDSRVSPT
Sbjct: 41 LQGCHPTPETAEQRIIDGFTYFKIHNFDKNPDLYK-ELAKGQSPKFLIFAC-SDSRVSPT 98
Query: 356 TILNFQPGEAFMVRNIANMVPPFNQLRYSGVG 451
TILNFQPGEAFMVRNIANMVPPFNQLRYSGVG
Sbjct: 99 TILNFQPGEAFMVRNIANMVPPFNQLRYSGVG 130
Score = 86.3 bits (212), Expect = 8e-17
Identities = 56/88 (63%), Positives = 60/88 (67%), Gaps = 3/88 (3%)
Frame = +1
Query: 55 MAKHSPDQLAIEELKKLIREKEELNESADTKIEDVIAELQRAGVSPYS*NC*AENH---G 225
MAKHSPDQLAIEELKKLIREKEELNESADTKIEDVIAELQ G P AE G
Sbjct: 1 MAKHSPDQLAIEELKKLIREKEELNESADTKIEDVIAELQLQGCHPTPET--AEQRIIDG 58
Query: 226 WVYLLQDPQFRQEPGSSTRELAKGHSPK 309
+ Y + F + P +ELAKG SPK
Sbjct: 59 FTY-FKIHNFDKNP-DLYKELAKGQSPK 84
>pir||T09570 carbonate dehydratase (EC 4.2.1.1) - alfalfa
gi|1938227|emb|CAA63712.1| Carbonic anhydrase [Medicago
sativa]
Length = 266
Score = 130 bits (326), Expect = 5e-30
Identities = 65/97 (67%), Positives = 77/97 (79%), Gaps = 1/97 (1%)
Frame = +2
Query: 164 LSCSVQGCHPTP-ETAEQRIMDGFTYFKIHNFDKNPDLVQGNWLRATAPRFLSICCGSDS 340
L +QGCHP P + AEQRI+DGFTYFK++NF+KNP+L + +P+F+ C SDS
Sbjct: 34 LIVELQGCHPQPIDPAEQRIIDGFTYFKLNNFNKNPELYD-RLAKGQSPKFMVFAC-SDS 91
Query: 341 RVSPTTILNFQPGEAFMVRNIANMVPPFNQLRYSGVG 451
RVSP+ ILNFQPGEAF VRNIANMVPPFNQLRYSGVG
Sbjct: 92 RVSPSIILNFQPGEAFKVRNIANMVPPFNQLRYSGVG 128
Score = 56.6 bits (135), Expect = 7e-08
Identities = 41/85 (48%), Positives = 49/85 (57%)
Frame = +1
Query: 55 MAKHSPDQLAIEELKKLIREKEELNESADTKIEDVIAELQRAGVSPYS*NC*AENHGWVY 234
MA S +LAIE+LKKL+REKEELNE A TKIE++I ELQ P G+ Y
Sbjct: 1 MANQS-SELAIEQLKKLLREKEELNEVATTKIEELIVELQGCHPQPIDPAEQRIIDGFTY 59
Query: 235 LLQDPQFRQEPGSSTRELAKGHSPK 309
+ F + P R LAKG SPK
Sbjct: 60 FKLN-NFNKNPELYDR-LAKGQSPK 82
>emb|CAB43571.1| carbonic anhydrase [Glycine max]
Length = 261
Score = 125 bits (313), Expect = 2e-28
Identities = 67/97 (69%), Positives = 73/97 (75%), Gaps = 1/97 (1%)
Frame = +2
Query: 164 LSCSVQGCHPTP-ETAEQRIMDGFTYFKIHNFDKNPDLVQGNWLRATAPRFLSICCGSDS 340
L +QGC P P E A QRI+DGFT+FKI+NFDKN DL P++L C SDS
Sbjct: 33 LIAELQGCGPRPYEPAVQRIVDGFTHFKINNFDKNSDLYS-QLANGQRPKYLVFSC-SDS 90
Query: 341 RVSPTTILNFQPGEAFMVRNIANMVPPFNQLRYSGVG 451
RVS TTILNFQPGEAFMVRNIANMVPPFNQLRYSGVG
Sbjct: 91 RVSATTILNFQPGEAFMVRNIANMVPPFNQLRYSGVG 127
Score = 52.0 bits (123), Expect = 2e-06
Identities = 28/47 (59%), Positives = 35/47 (73%)
Frame = +1
Query: 55 MAKHSPDQLAIEELKKLIREKEELNESADTKIEDVIAELQRAGVSPY 195
MAK S +LAIEELKKL+R+KEE + A K+E++IAELQ G PY
Sbjct: 1 MAKSS--ELAIEELKKLLRDKEEFDAVAAAKVEELIAELQGCGPRPY 45
>gb|AAD29049.1|AF132854_1 carbonic anhydrase isoform 1 [Gossypium hirsutum]
Length = 322
Score = 91.3 bits (225), Expect = 2e-18
Identities = 39/84 (46%), Positives = 64/84 (75%)
Frame = +2
Query: 200 ETAEQRIMDGFTYFKIHNFDKNPDLVQGNWLRATAPRFLSICCGSDSRVSPTTILNFQPG 379
+++ +R+ +GF YFK ++KNP L G + +P+++ + C SDSRV P+ +L+ QPG
Sbjct: 112 DSSVERLKEGFVYFKKEKYEKNPALY-GELAKGQSPKYMIVAC-SDSRVCPSHVLDMQPG 169
Query: 380 EAFMVRNIANMVPPFNQLRYSGVG 451
EAF+VRN+ANMVPP++Q++Y+G+G
Sbjct: 170 EAFVVRNVANMVPPYDQIKYAGIG 193
Score = 40.8 bits (94), Expect = 0.004
Identities = 32/89 (35%), Positives = 47/89 (51%), Gaps = 2/89 (2%)
Frame = +1
Query: 49 KNMAKHSPDQLAIEELKKLIREKEELNESADTKIEDVIAELQ--RAGVSPYS*NC*AENH 222
+ M S D+ AIE LKKL+ EK EL A +++ + AEL A P +
Sbjct: 62 EEMGNKSYDE-AIEALKKLLSEKGELKAEAAARVDQITAELNTTSADGKPSDSSVERLKE 120
Query: 223 GWVYLLQDPQFRQEPGSSTRELAKGHSPK 309
G+VY ++ ++ + P + ELAKG SPK
Sbjct: 121 GFVYFKKE-KYEKNP-ALYGELAKGQSPK 147
>gb|AAM22683.1|AF482951_1 carbonic anhydrase [Gossypium hirsutum]
Length = 326
Score = 91.3 bits (225), Expect = 2e-18
Identities = 39/84 (46%), Positives = 64/84 (75%)
Frame = +2
Query: 200 ETAEQRIMDGFTYFKIHNFDKNPDLVQGNWLRATAPRFLSICCGSDSRVSPTTILNFQPG 379
+++ +R+ +GF YFK ++KNP L G + +P+++ + C SDSRV P+ +L+ QPG
Sbjct: 116 DSSVERLKEGFVYFKKEKYEKNPALY-GELAKGQSPKYMIVAC-SDSRVCPSHVLDMQPG 173
Query: 380 EAFMVRNIANMVPPFNQLRYSGVG 451
EAF+VRN+ANMVPP++Q++Y+G+G
Sbjct: 174 EAFVVRNVANMVPPYDQIKYAGIG 197
Score = 42.4 bits (98), Expect = 0.001
Identities = 32/89 (35%), Positives = 47/89 (51%), Gaps = 2/89 (2%)
Frame = +1
Query: 49 KNMAKHSPDQLAIEELKKLIREKEELNESADTKIEDVIAELQRAGV--SPYS*NC*AENH 222
+ M S D+ AIE LKKL+ EK EL A +++ + AEL A P +
Sbjct: 66 EEMGNKSYDE-AIEALKKLLSEKGELKAEAAARVDQITAELNTASADGKPSDSSVERLKE 124
Query: 223 GWVYLLQDPQFRQEPGSSTRELAKGHSPK 309
G+VY ++ ++ + P + ELAKG SPK
Sbjct: 125 GFVYFKKE-KYEKNP-ALYGELAKGQSPK 151
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 393,847,897
Number of Sequences: 1393205
Number of extensions: 7721892
Number of successful extensions: 24505
Number of sequences better than 10.0: 176
Number of HSP's better than 10.0 without gapping: 23744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24455
length of database: 448,689,247
effective HSP length: 125
effective length of database: 274,538,622
effective search space used: 6588926928
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
