Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003717A_C01 KMC003717A_c01
(572 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_200637.1| 26S proteasome AAA-ATPase subunit RPT3; protein... 197 1e-49
sp|P54778|PRS6_SOLTU 26S PROTEASE REGULATORY SUBUNIT 6B HOMOLOG ... 189 2e-47
ref|NP_572686.1| CG16916-PA [Drosophila melanogaster] gi|6434950... 168 4e-41
ref|NP_006494.1| proteasome 26S ATPase subunit 4 isoform 1; prot... 168 5e-41
ref|NP_476463.1| proteasome 26S ATPase subunit 4 [Rattus norveg... 168 5e-41
>ref|NP_200637.1| 26S proteasome AAA-ATPase subunit RPT3; protein id: At5g58290.1,
supported by cDNA: gi_17979230, supported by cDNA:
gi_6652881 [Arabidopsis thaliana]
gi|28558168|sp|Q9SEI4|PRS6_ARATH 26S protease regulatory
subunit 6B homolog (26S proteasome AAA-ATPase subunit
RPT3) (Regulatory particle triple-A ATPase subunit 3)
gi|6652882|gb|AAF22523.1|AF123392_1 26S proteasome
AAA-ATPase subunit RPT3 [Arabidopsis thaliana]
gi|8777330|dbj|BAA96920.1| 26S proteasome AAA-ATPase
subunit RPT3 [Arabidopsis thaliana]
gi|17979231|gb|AAL49932.1| AT4g10340/F24G24_140
[Arabidopsis thaliana]
Length = 408
Score = 197 bits (500), Expect = 1e-49
Identities = 106/134 (79%), Positives = 114/134 (84%)
Frame = +2
Query: 101 SAMVLDPKPLSEPPPSLQSAKSDYLSGADADEDDLYSRLKTLQRQLEFIDIQEEYVKEEQ 280
++MVLDPK P+L + ADE+DLY RLK+L+RQLEF DIQEEYVK+EQ
Sbjct: 7 ASMVLDPKA----SPALMDLST-------ADEEDLYGRLKSLERQLEFTDIQEEYVKDEQ 55
Query: 281 KNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDTNNGIVGSTTGSNYYVRILSTINRELLK 460
KNLKRELLRAQEEVKRIQSVPLVIGQFMEMVD NNGIVGSTTGSNYYVRILSTINRELLK
Sbjct: 56 KNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLK 115
Query: 461 PSASVALHRHSNAL 502
PSASVALHRHSNAL
Sbjct: 116 PSASVALHRHSNAL 129
Score = 50.1 bits (118), Expect = 2e-05
Identities = 29/48 (60%), Positives = 33/48 (68%), Gaps = 3/48 (6%)
Frame = +3
Query: 438 LLTVSSSSLLPPSPSTVTPTPS---LDVLPPEADSSISLLSQSEKPDV 572
+L+ + LL PS S S +DVLPPEADSSISLLSQSEKPDV
Sbjct: 105 ILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDV 152
>sp|P54778|PRS6_SOLTU 26S PROTEASE REGULATORY SUBUNIT 6B HOMOLOG gi|7435738|pir||T07110
vacuolar proton-ATPase chain E - potato
gi|1155334|gb|AAB67835.1| POTATP1
Length = 413
Score = 189 bits (480), Expect = 2e-47
Identities = 103/140 (73%), Positives = 113/140 (80%), Gaps = 5/140 (3%)
Frame = +2
Query: 98 SSAMVLDPKPLSE-----PPPSLQSAKSDYLSGADADEDDLYSRLKTLQRQLEFIDIQEE 262
++AMVLDPKP + P S+ SD EDDLY+RLK+LQRQLEFI+IQEE
Sbjct: 2 ATAMVLDPKPAEKLPATRPETSITDVPSD-------GEDDLYARLKSLQRQLEFIEIQEE 54
Query: 263 YVKEEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDTNNGIVGSTTGSNYYVRILSTI 442
YVK+E KNL+RE LRAQEEVKRIQSVPLVIGQFMEM+D NN IVGSTTGSNYYVRILSTI
Sbjct: 55 YVKDELKNLRREHLRAQEEVKRIQSVPLVIGQFMEMIDQNNAIVGSTTGSNYYVRILSTI 114
Query: 443 NRELLKPSASVALHRHSNAL 502
NRELLKPSASV L RHSNAL
Sbjct: 115 NRELLKPSASVGLDRHSNAL 134
Score = 49.3 bits (116), Expect = 3e-05
Identities = 29/48 (60%), Positives = 33/48 (68%), Gaps = 3/48 (6%)
Frame = +3
Query: 438 LLTVSSSSLLPPSPSTVTPTPS---LDVLPPEADSSISLLSQSEKPDV 572
+L+ + LL PS S S +DVLPPEADSSISLLSQSEKPDV
Sbjct: 110 ILSTINRELLKPSASVGLDRHSNALVDVLPPEADSSISLLSQSEKPDV 157
>ref|NP_572686.1| CG16916-PA [Drosophila melanogaster]
gi|6434950|gb|AAF08387.1|AF145306_1 26S proteasome
regulatory complex subunit p48A [Drosophila
melanogaster] gi|7292602|gb|AAF48001.1| CG16916-PA
[Drosophila melanogaster]
Length = 413
Score = 168 bits (426), Expect = 4e-41
Identities = 85/114 (74%), Positives = 98/114 (85%)
Frame = +2
Query: 161 KSDYLSGADADEDDLYSRLKTLQRQLEFIDIQEEYVKEEQKNLKRELLRAQEEVKRIQSV 340
K + S + D +DLY R K LQ+ LEFI++QEEY+K+EQ+NLK+E L AQEEVKRIQSV
Sbjct: 21 KDAHSSLDELDMEDLYVRYKKLQKTLEFIEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSV 80
Query: 341 PLVIGQFMEMVDTNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNAL 502
PLVIGQF+E VD N GIVGSTTGSNYYVRILSTI+RELLKPSASVALH+HSNAL
Sbjct: 81 PLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNAL 134
Score = 43.9 bits (102), Expect = 0.001
Identities = 28/58 (48%), Positives = 35/58 (60%), Gaps = 6/58 (10%)
Frame = +3
Query: 417 TTFGSSVLLTVSSS---SLLPPSPSTVTPTPS---LDVLPPEADSSISLLSQSEKPDV 572
+T GS+ + + S+ LL PS S S +DVLPPEADSSIS+L EKPDV
Sbjct: 100 STTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQPDEKPDV 157
>ref|NP_006494.1| proteasome 26S ATPase subunit 4 isoform 1; protease 26S subunit 6;
Tat-binding protein 7; MB67 interacting protein [Homo
sapiens] gi|20532409|sp|P43686|PRS6_HUMAN 26S protease
regulatory subunit 6B (MIP224) (MB67 interacting
protein) (TAT-binding protein-7) (TBP-7)
gi|2791680|gb|AAC26843.1| 26S proteasome ATPase subunit
[Homo sapiens] gi|4096275|gb|AAC99817.1| MIP224 [Homo
sapiens] gi|5080757|gb|AAD39267.1|AC007842_2 ATPase
homolog [Homo sapiens]; MIP22; TAT-BINDING PROTEIN-7;
TBP-7; 26S PROTEASE REGULATORY SUBUNIT 6B
gi|12653151|gb|AAH00343.1|AAH00343 proteasome (prosome,
macropain) 26S subunit, ATPase, 4 [Homo sapiens]
gi|15680265|gb|AAH14488.1|AAH14488 proteasome (prosome,
macropain) 26S subunit, ATPase, 4 [Homo sapiens]
Length = 418
Score = 168 bits (425), Expect = 5e-41
Identities = 82/111 (73%), Positives = 97/111 (86%)
Frame = +2
Query: 170 YLSGADADEDDLYSRLKTLQRQLEFIDIQEEYVKEEQKNLKRELLRAQEEVKRIQSVPLV 349
+L D +DLYSR K LQ++LEF+++QEEY+K+EQKNLK+E L AQEEVKRIQS+PLV
Sbjct: 29 FLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLV 88
Query: 350 IGQFMEMVDTNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNAL 502
IGQF+E VD N IVGSTTGSNYYVRILSTI+RELLKP+ASVALH+HSNAL
Sbjct: 89 IGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNAL 139
Score = 40.4 bits (93), Expect = 0.016
Identities = 25/58 (43%), Positives = 35/58 (60%), Gaps = 6/58 (10%)
Frame = +3
Query: 417 TTFGSSVLLTVSSS---SLLPPSPSTVTPTPS---LDVLPPEADSSISLLSQSEKPDV 572
+T GS+ + + S+ LL P+ S S +DVLPPEADSSI +L+ +KPDV
Sbjct: 105 STTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDV 162
>ref|NP_476463.1| proteasome 26S ATPase subunit 4 [Rattus norvegicus]
gi|2492517|sp|Q63570|PRS6_RAT 26S protease regulatory
subunit 6B (TAT-binding protein-7) (TBP-7)
gi|1395186|dbj|BAA09340.1| proteasomal ATPase
(Tat-binding protein7) [Rattus norvegicus]
gi|15215225|gb|AAH12708.1| similar to proteasome
(prosome, macropain) 26S subunit, ATPase, 4 [Mus
musculus] gi|26346368|dbj|BAC36835.1| unnamed protein
product [Mus musculus]
Length = 418
Score = 168 bits (425), Expect = 5e-41
Identities = 82/111 (73%), Positives = 97/111 (86%)
Frame = +2
Query: 170 YLSGADADEDDLYSRLKTLQRQLEFIDIQEEYVKEEQKNLKRELLRAQEEVKRIQSVPLV 349
+L D +DLYSR K LQ++LEF+++QEEY+K+EQKNLK+E L AQEEVKRIQS+PLV
Sbjct: 29 FLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLV 88
Query: 350 IGQFMEMVDTNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNAL 502
IGQF+E VD N IVGSTTGSNYYVRILSTI+RELLKP+ASVALH+HSNAL
Sbjct: 89 IGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNAL 139
Score = 40.4 bits (93), Expect = 0.016
Identities = 25/58 (43%), Positives = 35/58 (60%), Gaps = 6/58 (10%)
Frame = +3
Query: 417 TTFGSSVLLTVSSS---SLLPPSPSTVTPTPS---LDVLPPEADSSISLLSQSEKPDV 572
+T GS+ + + S+ LL P+ S S +DVLPPEADSSI +L+ +KPDV
Sbjct: 105 STTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDV 162
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 561,075,451
Number of Sequences: 1393205
Number of extensions: 14215557
Number of successful extensions: 90491
Number of sequences better than 10.0: 609
Number of HSP's better than 10.0 without gapping: 70967
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 88497
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21243732558
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
