Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003711A_C01 KMC003711A_c01
(535 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_173823.1| G-protein beta family; protein id: At1g24130.1 ... 184 6e-46
ref|NP_564219.1| G-protein beta family; protein id: At1g24530.1,... 163 1e-39
ref|NP_199823.1| G-protein beta family; protein id: At5g50120.1 ... 143 1e-33
ref|NP_175369.1| En/Spm-like transposon protein, putative; prote... 118 5e-26
gb|AAL49946.1| At1g49450/F13F21_11 [Arabidopsis thaliana] gi|250... 118 5e-26
>ref|NP_173823.1| G-protein beta family; protein id: At1g24130.1 [Arabidopsis
thaliana] gi|7486376|pir||T00642 hypothetical protein
F3I6.5 - Arabidopsis thaliana gi|2829890|gb|AAC00598.1|
Hypothetical protein [Arabidopsis thaliana]
Length = 415
Score = 184 bits (467), Expect = 6e-46
Identities = 98/158 (62%), Positives = 116/158 (73%), Gaps = 4/158 (2%)
Frame = -3
Query: 518 EKKHSLLETLEKHKSAVNALALNEDGSVLYSGACDRSILVWER--DGGDGGGEMVVVGAL 345
+KKHSL+ TL KH SAVNALA++EDG VLYSGACDRSILVWER +G D M VVGAL
Sbjct: 265 DKKHSLVATLTKHLSAVNALAISEDGKVLYSGACDRSILVWERLINGDDEELHMSVVGAL 324
Query: 344 RGHTKAILCLVVVGDLVFSGSADNSVRVWRRGV--GKSYACVAVLEGHRRPVKCLAVAVA 171
RGH KAI+CL V DLV SGSAD S+RVWRRG+ + Y+C+AVLEGH +PVK LAV+V+
Sbjct: 325 RGHRKAIMCLAVASDLVLSGSADKSLRVWRRGLMEKEGYSCLAVLEGHTKPVKSLAVSVS 384
Query: 170 VDFNNGGGPGGDDESSSSSCLVYSGSLDCDIKVWRIRV 57
D +S SC+VYSGSLD +KVW +RV
Sbjct: 385 ----------DSDSNSDYSCMVYSGSLDLSLKVWNLRV 412
Score = 50.1 bits (118), Expect = 2e-05
Identities = 49/174 (28%), Positives = 73/174 (41%), Gaps = 26/174 (14%)
Frame = -3
Query: 509 HSLLETLEKHKSAVNALALNEDGSVLYSGACDRSILVWERDGG-----DGGGEMVVVGAL 345
H L TL+ S V++LA+++ +LY+G+ + I VW R+ G + VV
Sbjct: 59 HHCLATLKDKSSYVSSLAVSD--KLLYTGSSNSEIRVWPREPPFSPEYSTGDDRNVVANG 116
Query: 344 RGHTKAILCLVVVGDLVFSGSADNSVRVWR-----RGVGKSYACVAVLEGH--------- 207
G K+ LV++GD + S D+ +RVW+ G+ Y CVA L
Sbjct: 117 NGGVKS---LVILGDKLISAHQDHKIRVWKIIDESNRRGQKYKCVATLPTMNDRFKTLFS 173
Query: 206 -------RRPVKCLAVAVAVDFNNGGGPGGDDESSSSSCLVYSGSLDCDIKVWR 66
RR KC V ++ S L+YS S D K+WR
Sbjct: 174 SKSYVEVRRHKKCTWVHHVDAVSSLA-------LSQDGSLLYSASWDRSFKIWR 220
>ref|NP_564219.1| G-protein beta family; protein id: At1g24530.1, supported by cDNA:
gi_19310648 [Arabidopsis thaliana]
gi|9743339|gb|AAF97963.1|AC000103_13 F21J9.19
[Arabidopsis thaliana] gi|15028341|gb|AAK76647.1|
unknown protein [Arabidopsis thaliana]
gi|19310649|gb|AAL85055.1| unknown protein [Arabidopsis
thaliana]
Length = 418
Score = 163 bits (412), Expect = 1e-39
Identities = 88/155 (56%), Positives = 107/155 (68%)
Frame = -3
Query: 521 GEKKHSLLETLEKHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVVGALR 342
GEK+H+L+ TLEKHKSAVNALALN+DGSVL+SG+CDRSILVWER+ D M V GALR
Sbjct: 266 GEKRHTLVATLEKHKSAVNALALNDDGSVLFSGSCDRSILVWERE--DTSNYMAVRGALR 323
Query: 341 GHTKAILCLVVVGDLVFSGSADNSVRVWRRGVGKSYACVAVLEGHRRPVKCLAVAVAVDF 162
GH KAIL L V DL+ SGSAD +VR+WRRG SY+C+ VL GH +PVK LA +
Sbjct: 324 GHDKAILSLFNVSDLLLSGSADRTVRIWRRGPDSSYSCLEVLSGHTKPVKSLAAVREKEL 383
Query: 161 NNGGGPGGDDESSSSSCLVYSGSLDCDIKVWRIRV 57
DD S + SGSLD ++K W++ V
Sbjct: 384 --------DDVVS-----IISGSLDGEVKCWKVSV 405
Score = 55.5 bits (132), Expect = 4e-07
Identities = 41/141 (29%), Positives = 68/141 (48%), Gaps = 2/141 (1%)
Frame = -3
Query: 485 KHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVVGALRGHTKAILCLVV- 309
+H AV ALA+++ +YS + D+++ +W ++ +++ H A+ + V
Sbjct: 193 EHADAVTALAVSD--GFIYSVSWDKTLKIWR------ASDLRCKESIKAHDDAVNAIAVS 244
Query: 308 VGDLVFSGSADNSVRVWRRGVG-KSYACVAVLEGHRRPVKCLAVAVAVDFNNGGGPGGDD 132
V++GSAD +RVW + G K + VA LE H+ V LA+ DD
Sbjct: 245 TNGTVYTGSADRRIRVWAKPTGEKRHTLVATLEKHKSAVNALAL-------------NDD 291
Query: 131 ESSSSSCLVYSGSLDCDIKVW 69
S +++SGS D I VW
Sbjct: 292 GS-----VLFSGSCDRSILVW 307
>ref|NP_199823.1| G-protein beta family; protein id: At5g50120.1 [Arabidopsis
thaliana] gi|10177223|dbj|BAB10298.1| contains
similarity to GTP-binding regulatory protein and
WD-repeat protein~gene_id:MPF21.14 [Arabidopsis
thaliana]
Length = 388
Score = 143 bits (361), Expect = 1e-33
Identities = 76/156 (48%), Positives = 105/156 (66%), Gaps = 1/156 (0%)
Frame = -3
Query: 518 EKKHSLLETLEKHKSAVNALALN-EDGSVLYSGACDRSILVWERDGGDGGGEMVVVGALR 342
++KHSL+ L +H S +NALAL+ +GS+L+SG D SILVWERD G G++VVVG LR
Sbjct: 246 KRKHSLVAILSEHNSGINALALSGTNGSLLHSGGSDGSILVWERDDG---GDIVVVGMLR 302
Query: 341 GHTKAILCLVVVGDLVFSGSADNSVRVWRRGVGKSYACVAVLEGHRRPVKCLAVAVAVDF 162
GHT+++LCL VV D++ SGSAD +VR+W+ K Y+C+A+LEGH PVKCL
Sbjct: 303 GHTESVLCLAVVSDILCSGSADKTVRLWKCS-AKDYSCLAMLEGHLGPVKCLT------- 354
Query: 161 NNGGGPGGDDESSSSSCLVYSGSLDCDIKVWRIRVP 54
G ++ +S +YSG LD +KVW++ VP
Sbjct: 355 ---GAFRDSRKADEASYHIYSGGLDSQVKVWQVLVP 387
>ref|NP_175369.1| En/Spm-like transposon protein, putative; protein id: At1g49450.1,
supported by cDNA: gi_17979258 [Arabidopsis thaliana]
gi|25405310|pir||B96531 hypothetical protein F13F21.11
[imported] - Arabidopsis thaliana
gi|5430755|gb|AAD43155.1|AC007504_10 Hypothetical
Protein [Arabidopsis thaliana]
Length = 471
Score = 118 bits (295), Expect = 5e-26
Identities = 65/158 (41%), Positives = 91/158 (57%), Gaps = 1/158 (0%)
Frame = -3
Query: 533 KVHAGEKKHSLLETLEKHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVV 354
+V E KH L++ L K ++AV ALA+N +V+Y G+ D ++ WER +
Sbjct: 316 EVQGKEMKHVLVQVLMKQENAVTALAVNLTDAVVYCGSSDGTVNFWERQK-----YLTHK 370
Query: 353 GALRGHTKAILCLVVVGDLVFSGSADNSVRVWRRGVGKSYACVAVLEGHRRPVKCL-AVA 177
G + GH A+LCL G L+ SG AD ++ VW+R S+ C++VL H PVKCL AV
Sbjct: 371 GTIHGHRMAVLCLATAGSLLLSGGADKNICVWKRNGDGSHTCLSVLMDHEGPVKCLAAVE 430
Query: 176 VAVDFNNGGGPGGDDESSSSSCLVYSGSLDCDIKVWRI 63
A + +N G GG E +VYSGSLD +KVWR+
Sbjct: 431 EAEEDHNDGDDGG--EKGDQRWIVYSGSLDNSVKVWRV 466
Score = 60.1 bits (144), Expect = 2e-08
Identities = 45/143 (31%), Positives = 69/143 (47%), Gaps = 4/143 (2%)
Frame = -3
Query: 485 KHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVVGALRGHTKAILCLVV- 309
+H AV+ L+LNED +LYSG+ D+++ VW + + ++ H A+ +V
Sbjct: 243 RHFDAVSCLSLNEDLGLLYSGSWDKTLKVWRL------SDSKCLESIEAHDDAVNTVVSG 296
Query: 308 VGDLVFSGSADNSVRVWRRGV-GK--SYACVAVLEGHRRPVKCLAVAVAVDFNNGGGPGG 138
DLVF+GSAD +++VW+R V GK + V VL V LAV
Sbjct: 297 FDDLVFTGSADGTLKVWKREVQGKEMKHVLVQVLMKQENAVTALAV-------------- 342
Query: 137 DDESSSSSCLVYSGSLDCDIKVW 69
+ + +VY GS D + W
Sbjct: 343 ----NLTDAVVYCGSSDGTVNFW 361
Score = 37.4 bits (85), Expect = 0.11
Identities = 44/160 (27%), Positives = 73/160 (45%), Gaps = 11/160 (6%)
Frame = -3
Query: 509 HSLLETLEKHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVVGALRGHTK 330
+ L+ T+ + + V +LA + G +L++G+ ++I VW +D D G + G K
Sbjct: 124 NGLIGTVVRQEGHVYSLAAS--GDLLFTGSDSKNIRVW-KDLKDFSG----FKSTSGFVK 176
Query: 329 AILCLVVVGDLVFSGSADNSVRVWR--RGVGKSYACVAVLEGHRR-PVKCLAVAVAVDFN 159
AI+ V + VF+G D +RVWR + + Y+ V L + K + V+
Sbjct: 177 AIV--VTRDNRVFTGHQDGKIRVWRGSKKNPEKYSRVGSLPTLKEFLTKSVNPRNYVEVR 234
Query: 158 NGGGPGGDDESSSSSC--------LVYSGSLDCDIKVWRI 63
+ SC L+YSGS D +KVWR+
Sbjct: 235 RRKNVLKIRHFDAVSCLSLNEDLGLLYSGSWDKTLKVWRL 274
>gb|AAL49946.1| At1g49450/F13F21_11 [Arabidopsis thaliana]
gi|25090346|gb|AAN72281.1| At1g49450/F13F21_11
[Arabidopsis thaliana]
Length = 471
Score = 118 bits (295), Expect = 5e-26
Identities = 65/158 (41%), Positives = 91/158 (57%), Gaps = 1/158 (0%)
Frame = -3
Query: 533 KVHAGEKKHSLLETLEKHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVV 354
+V E KH L++ L K ++AV ALA+N +V+Y G+ D ++ WER +
Sbjct: 316 EVQGKEMKHVLVQVLMKQENAVTALAVNLTDAVVYCGSSDGTVNFWERQK-----YLTHK 370
Query: 353 GALRGHTKAILCLVVVGDLVFSGSADNSVRVWRRGVGKSYACVAVLEGHRRPVKCL-AVA 177
G + GH A+LCL G L+ SG AD ++ VW+R S+ C++VL H PVKCL AV
Sbjct: 371 GTIHGHRMAVLCLATAGSLLLSGGADKNICVWKRNGDGSHTCLSVLMDHEGPVKCLAAVE 430
Query: 176 VAVDFNNGGGPGGDDESSSSSCLVYSGSLDCDIKVWRI 63
A + +N G GG E +VYSGSLD +KVWR+
Sbjct: 431 EAEEDHNDGDDGG--EKGDQRWIVYSGSLDNSVKVWRV 466
Score = 60.1 bits (144), Expect = 2e-08
Identities = 45/143 (31%), Positives = 69/143 (47%), Gaps = 4/143 (2%)
Frame = -3
Query: 485 KHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVVGALRGHTKAILCLVV- 309
+H AV+ L+LNED +LYSG+ D+++ VW + + ++ H A+ +V
Sbjct: 243 RHFDAVSCLSLNEDLGLLYSGSWDKTLKVWRL------SDSKCLESIEAHDDAVNTVVSG 296
Query: 308 VGDLVFSGSADNSVRVWRRGV-GK--SYACVAVLEGHRRPVKCLAVAVAVDFNNGGGPGG 138
DLVF+GSAD +++VW+R V GK + V VL V LAV
Sbjct: 297 FDDLVFTGSADGTLKVWKREVQGKEMKHVLVQVLMKQENAVTALAV-------------- 342
Query: 137 DDESSSSSCLVYSGSLDCDIKVW 69
+ + +VY GS D + W
Sbjct: 343 ----NLTDAVVYCGSSDGTVNFW 361
Score = 35.8 bits (81), Expect = 0.33
Identities = 27/85 (31%), Positives = 46/85 (53%)
Frame = -3
Query: 509 HSLLETLEKHKSAVNALALNEDGSVLYSGACDRSILVWERDGGDGGGEMVVVGALRGHTK 330
+ L+ T+ + + V +LA + G +L++G+ ++I VW +D D G + G K
Sbjct: 124 NGLIGTVVRQEGHVYSLAAS--GDLLFTGSDSKNIRVW-KDLKDFSG----FKSTSGFVK 176
Query: 329 AILCLVVVGDLVFSGSADNSVRVWR 255
AI+ V + VF+G D +RVWR
Sbjct: 177 AIV--VTRDNRVFTGHQDGKIRVWR 199
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 621,900,206
Number of Sequences: 1393205
Number of extensions: 20714262
Number of successful extensions: 308517
Number of sequences better than 10.0: 8275
Number of HSP's better than 10.0 without gapping: 98567
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198034
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17885181664
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
