Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003684A_C01 KMC003684A_c01
(513 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T06325 malate dehydrogenase (EC 1.1.1.37), nodule-enhanced ... 177 2e-54
pir||T09294 malate dehydrogenase (EC 1.1.1.37) precursor - alfal... 176 1e-49
emb|CAB45387.1| NAD-malate dehydrogenase [Nicotiana tabacum] 153 1e-44
pir||T06386 probable malate dehydrogenase (EC 1.1.1.37) - garden... 155 3e-37
dbj|BAB68116.1| putative NAD-malate dehydrogenase [Oryza sativa ... 135 3e-31
>pir||T06325 malate dehydrogenase (EC 1.1.1.37), nodule-enhanced - soybean
gi|3273828|gb|AAC24855.1| nodule-enhanced malate
dehydrogenase [Glycine max]
Length = 413
Score = 177 bits (450), Expect(2) = 2e-54
Identities = 91/111 (81%), Positives = 100/111 (89%), Gaps = 2/111 (1%)
Frame = +2
Query: 185 AFCGLRAVSSVRCEVESSFSGKETGSALRASFATKAQKESRNQ--HQLQPQASYKVAVLG 358
+FCGL+A+SSVRCE ESSF +TG+ALRASFA+KAQKE+ + QPQASYKVAVLG
Sbjct: 42 SFCGLKAMSSVRCESESSFLVNKTGAALRASFASKAQKENDQNFNYNSQPQASYKVAVLG 101
Query: 359 AAGGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTG 511
AAGGIGQPLALLIKMSPL+SDLHLYDIANVKGVAADISHCNTPSQVRDFTG
Sbjct: 102 AAGGIGQPLALLIKMSPLISDLHLYDIANVKGVAADISHCNTPSQVRDFTG 152
Score = 56.6 bits (135), Expect(2) = 2e-54
Identities = 29/40 (72%), Positives = 31/40 (77%)
Frame = +1
Query: 61 MAATSAATFTIGAPTSLYQRGSSLPQSKSFGLRFTSQNHL 180
MAA AATFTIG SL QRG+SLPQ KS GL+F SQNHL
Sbjct: 1 MAAAPAATFTIGTTGSLGQRGNSLPQLKSSGLKFNSQNHL 40
>pir||T09294 malate dehydrogenase (EC 1.1.1.37) precursor - alfalfa
gi|2827084|gb|AAB99757.1| malate dehydrogenase precursor
[Medicago sativa]
Length = 408
Score = 176 bits (445), Expect(2) = 1e-49
Identities = 91/109 (83%), Positives = 99/109 (90%), Gaps = 1/109 (0%)
Frame = +2
Query: 188 FCGLRAVSSVRCEVESSFSGKETGSALRASFATKAQKESRN-QHQLQPQASYKVAVLGAA 364
F GL+A+ S+RC+ ESSFSGKET +ALRA+FA KAQKE+RN LQPQASYKVAVLGAA
Sbjct: 39 FSGLKAMPSLRCDSESSFSGKETCAALRATFAPKAQKENRNLTRNLQPQASYKVAVLGAA 98
Query: 365 GGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTG 511
GGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPS+V DFTG
Sbjct: 99 GGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSKVLDFTG 147
Score = 42.4 bits (98), Expect(2) = 1e-49
Identities = 23/41 (56%), Positives = 27/41 (65%)
Frame = +1
Query: 61 MAATSAATFTIGAPTSLYQRGSSLPQSKSFGLRFTSQNHLN 183
MAA ++ATFTIG Q G SLPQS FGL+F SQ + N
Sbjct: 1 MAAAASATFTIGTT----QTGRSLPQSSPFGLKFNSQVNFN 37
>emb|CAB45387.1| NAD-malate dehydrogenase [Nicotiana tabacum]
Length = 412
Score = 153 bits (387), Expect(2) = 1e-44
Identities = 77/110 (70%), Positives = 93/110 (84%), Gaps = 1/110 (0%)
Frame = +2
Query: 185 AFCGLRAVSSVRCEVESSFSGKETGSALRASFATKAQKESRNQHQ-LQPQASYKVAVLGA 361
+F GL+A + V CE ESSF GKE+ + L+ SF KAQKE++ +QPQASYKVAVLGA
Sbjct: 42 SFSGLKAATDVSCESESSFLGKESVAVLKQSFTPKAQKENKGYGNCVQPQASYKVAVLGA 101
Query: 362 AGGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTG 511
+GGIGQPL+LL+KMSPLVS+L+LYDIANVKGVAAD+SHCNTPS+V DFTG
Sbjct: 102 SGGIGQPLSLLVKMSPLVSELNLYDIANVKGVAADLSHCNTPSKVSDFTG 151
Score = 47.8 bits (112), Expect(2) = 1e-44
Identities = 23/40 (57%), Positives = 32/40 (79%)
Frame = +1
Query: 61 MAATSAATFTIGAPTSLYQRGSSLPQSKSFGLRFTSQNHL 180
MAATSA TF++G+ TSL +GSS+ QSK+F ++F S+N L
Sbjct: 1 MAATSATTFSVGSATSLGCKGSSVSQSKAFSVKFYSKNSL 40
>pir||T06386 probable malate dehydrogenase (EC 1.1.1.37) - garden pea
gi|3377762|gb|AAC28106.1| nodule-enhanced malate
dehydrogenase [Pisum sativum]
Length = 398
Score = 155 bits (392), Expect = 3e-37
Identities = 84/109 (77%), Positives = 93/109 (85%), Gaps = 1/109 (0%)
Frame = +2
Query: 188 FCGLRAVSSVRCEVESSFSGKETGSALRASFATKAQKESRNQ-HQLQPQASYKVAVLGAA 364
F GL+A+SS FSG ET +ALRA+FA KA KE++N+ H LQPQASYKVA+LGAA
Sbjct: 37 FSGLKAMSS--------FSGNETCAALRATFAPKAVKENQNRNHILQPQASYKVALLGAA 88
Query: 365 GGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTG 511
GGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPS+V DFTG
Sbjct: 89 GGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSKVADFTG 137
>dbj|BAB68116.1| putative NAD-malate dehydrogenase [Oryza sativa (japonica
cultivar-group)] gi|20160639|dbj|BAB89584.1| putative
NAD-malate dehydrogenase [Oryza sativa (japonica
cultivar-group)]
Length = 404
Score = 135 bits (340), Expect = 3e-31
Identities = 70/109 (64%), Positives = 86/109 (78%)
Frame = +2
Query: 185 AFCGLRAVSSVRCEVESSFSGKETGSALRASFATKAQKESRNQHQLQPQASYKVAVLGAA 364
++ GL+A SS+ E SSF G+ ++LR+S + + ++ + + P+ASYKVAVLGAA
Sbjct: 35 SYSGLKARSSISFESRSSFLGQN--ASLRSSISPRIVPKANSGSHISPEASYKVAVLGAA 92
Query: 365 GGIGQPLALLIKMSPLVSDLHLYDIANVKGVAADISHCNTPSQVRDFTG 511
GGIGQPL LLIKMSPLVS LHLYDIANVKGVAAD+SHCNTPSQV DFTG
Sbjct: 93 GGIGQPLGLLIKMSPLVSALHLYDIANVKGVAADLSHCNTPSQVLDFTG 141
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 457,512,775
Number of Sequences: 1393205
Number of extensions: 10340570
Number of successful extensions: 34729
Number of sequences better than 10.0: 235
Number of HSP's better than 10.0 without gapping: 32526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34584
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 16232377112
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
