>KMC003547A_C01 KMC003547A_c01
atcaacgatatatagctgctggtatatgTATAGCCAAGAATCTAAATAAAATGGAAAATT
ATTACAAAGTTTACAATTGGTTTAACAATGGCCAGCAACAATGCTACAACTATCATTCAT
ATTATTACTCGGACCATAGCAGTTTTCTCATAAAAAAATAAAAAGAAAACAGGGTAGATA
ACTTAATATCCACACTCTTTGAAATTGTGTACTTGTAACCTTGAAACCCACCATGTTTAA
TTGTGATTGGCATATCAAGTTACTTGTAATGCTGAGATACCTCCCAAACTAGCTTATCCA
TAAAATGGCATTTCTTGTCAAATCCCCAGCCTGGGTTGCTCTGAAGAGAGATAACAGCGC
GGTCAGTAAATTGCTTCACGGAAGATGGAGCGCTTGTGGGAAGAGTTTTAGCAACTCTCT
CTAATTCTCCTTGTTGCTTCTTTGCAGTTACCCTGTCTGGTCCACAATAGTGATCGACAA
TCTTTTTCATTAATGGAACTCGTGCCATTGTTTCTTCCATTGCTTCCTCCCACTCTTCTT
GACTTTGAATCCCCTCATATACCATCAAGAATGGTTTCATCTTCTCAAGTGCATCTCGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003547A_C01 KMC003547A_c01
         (599 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_564639.1| Expressed protein; protein id: At1g53645.1, sup...   170  1e-41
gb|AAM65660.1| Contains similarity to RNA-binding protein from A...   170  1e-41
gb|AAF78422.1|AC018748_1 Contains similarity to RNA-binding prot...   170  1e-41
ref|NP_036788.1| secreted acidic cystein-rich glycoprotein  (ost...    35  0.96
ref|NP_066539.1| haem lyase [Naegleria gruberi] gi|10444251|gb|A...    34  1.3

>ref|NP_564639.1| Expressed protein; protein id: At1g53645.1, supported by cDNA:
           41453., supported by cDNA: gi_16323138 [Arabidopsis
           thaliana] gi|25405656|pir||E96576 unknown protein,
           43598-45751 [imported] - Arabidopsis thaliana
           gi|12324041|gb|AAG51990.1|AC024260_28 unknown protein;
           43598-45751 [Arabidopsis thaliana]
           gi|16323139|gb|AAL15304.1| At1g53640/F22G10.8
           [Arabidopsis thaliana] gi|23506017|gb|AAN28868.1|
           At1g53640/F22G10.8 [Arabidopsis thaliana]
          Length = 523

 Score =  170 bits (431), Expect = 1e-41
 Identities = 79/112 (70%), Positives = 93/112 (82%)
 Frame = -2

Query: 598 RDALEKMKPFLMVYEGIQSQEEWEEAMEETMARVPLMKKIVDHYCGPDRVTAKKQQGELE 419
           R+ LEK+KPF++ YEGI+ QEEWEEA+ E M + PLMK+IVDHY GPDRVTAKKQ  EL+
Sbjct: 412 RECLEKVKPFIVAYEGIKDQEEWEEAINEAMTQAPLMKEIVDHYSGPDRVTAKKQNEELD 471

Query: 418 RVAKTLPTSAPSSVKQFTDRAVISLQSNPGWGFDKKCHFMDKLVWEVSQHYK 263
           R+A TLP SAP SVK+F DRA ++L+SNPGWGFDKK  FMDKLV EVSQ YK
Sbjct: 472 RIATTLPASAPDSVKRFADRAALTLKSNPGWGFDKKYQFMDKLVLEVSQSYK 523

>gb|AAM65660.1| Contains similarity to RNA-binding protein from Arabidopsis
           thaliana gi|2129727 and contains RNA recognition
           PF|00076 domain
          Length = 523

 Score =  170 bits (431), Expect = 1e-41
 Identities = 79/112 (70%), Positives = 93/112 (82%)
 Frame = -2

Query: 598 RDALEKMKPFLMVYEGIQSQEEWEEAMEETMARVPLMKKIVDHYCGPDRVTAKKQQGELE 419
           R+ LEK+KPF++ YEGI+ QEEWEEA+ E M + PLMK+IVDHY GPDRVTAKKQ  EL+
Sbjct: 412 RECLEKVKPFIVAYEGIKDQEEWEEAINEAMTQAPLMKEIVDHYSGPDRVTAKKQNEELD 471

Query: 418 RVAKTLPTSAPSSVKQFTDRAVISLQSNPGWGFDKKCHFMDKLVWEVSQHYK 263
           R+A TLP SAP SVK+F DRA ++L+SNPGWGFDKK  FMDKLV EVSQ YK
Sbjct: 472 RIATTLPASAPDSVKRFADRAALTLKSNPGWGFDKKYQFMDKLVLEVSQSYK 523

>gb|AAF78422.1|AC018748_1 Contains similarity to RNA-binding protein from Arabidopsis thaliana
            gi|2129727 and contains RNA recognition PF|00076 domain. 
            ESTs gb|H37317, gb|F14415, gb|AA651290 come from this
            gene
          Length = 829

 Score =  170 bits (431), Expect = 1e-41
 Identities = 79/112 (70%), Positives = 93/112 (82%)
 Frame = -2

Query: 598  RDALEKMKPFLMVYEGIQSQEEWEEAMEETMARVPLMKKIVDHYCGPDRVTAKKQQGELE 419
            R+ LEK+KPF++ YEGI+ QEEWEEA+ E M + PLMK+IVDHY GPDRVTAKKQ  EL+
Sbjct: 718  RECLEKVKPFIVAYEGIKDQEEWEEAINEAMTQAPLMKEIVDHYSGPDRVTAKKQNEELD 777

Query: 418  RVAKTLPTSAPSSVKQFTDRAVISLQSNPGWGFDKKCHFMDKLVWEVSQHYK 263
            R+A TLP SAP SVK+F DRA ++L+SNPGWGFDKK  FMDKLV EVSQ YK
Sbjct: 778  RIATTLPASAPDSVKRFADRAALTLKSNPGWGFDKKYQFMDKLVLEVSQSYK 829

>ref|NP_036788.1| secreted acidic cystein-rich glycoprotein  (osteonectin) [Rattus
           norvegicus] gi|2196884|emb|CAA74042.1| osteonectin
           [Rattus norvegicus]
          Length = 301

 Score = 34.7 bits (78), Expect = 0.96
 Identities = 23/78 (29%), Positives = 34/78 (43%)
 Frame = -2

Query: 535 EWEEAMEETMARVPLMKKIVDHYCGPDRVTAKKQQGELERVAKTLPTSAPSSVKQFTDRA 356
           E+EE  EET+  V       +H+C   +V    +      V +  PTS P+ + +F    
Sbjct: 52  EFEEGAEETVEEVVAENPCQNHHCKHGKVCELDESNTPMCVCQD-PTSCPAPIGEFE--- 107

Query: 355 VISLQSNPGWGFDKKCHF 302
              + SN    FD  CHF
Sbjct: 108 --KVCSNDNKTFDSSCHF 123

>ref|NP_066539.1| haem lyase [Naegleria gruberi]
           gi|10444251|gb|AAG17817.1|AF288092_42 haem lyase
           [Naegleria gruberi]
          Length = 474

 Score = 34.3 bits (77), Expect = 1.3
 Identities = 21/64 (32%), Positives = 36/64 (55%), Gaps = 3/64 (4%)
 Frame = +3

Query: 30  IAKNLNK-MENYYKVYNWFNNGQQQCYNY--HSYYYSDHSSFLIKK*KENRVDNLISTLF 200
           I KN NK + NYY +YN F   +   YNY  ++YYY+  ++ +    +  +++N+I    
Sbjct: 410 INKNKNKFISNYYNIYNIFEKIEFNIYNYIFNNYYYNIKNNVI----QIYKLNNIIVLFI 465

Query: 201 EIVY 212
            I+Y
Sbjct: 466 LILY 469

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 510,957,299
Number of Sequences: 1393205
Number of extensions: 10720684
Number of successful extensions: 32153
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 30733
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32113
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23426109484
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf049g11 BP071024 1 536 2 SPD006b02_f BP044447 29 609 3 SPDL066g11_f BP056123 30 429 4 SPD067e09_f BP049357 41 527 5 GNf054c09 BP071384 44 325 6 MWL006f01_f AV768677 44 298 7 SPD066c04_f BP049255 47 520 8 MFB012d03_f BP034790 47 614 9 SPD077c02_f BP050145 56 527 10 MRL042f02_f BP085772 88 543

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