Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003529A_C01 KMC003529A_c01
(631 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_565610.1| expressed protein; protein id: At2g25830.1, sup... 134 4e-31
gb|AAL07079.1| unknown protein [Arabidopsis thaliana] gi|2128120... 134 4e-31
pir||C84653 hypothetical protein At2g25830 [imported] - Arabidop... 127 6e-29
ref|NP_013018.1| Suppressor of mutant AC40 subunit of RNA polyme... 39 0.074
gb|AAA35091.1| selected as a weak suppressor of a mutant of the ... 39 0.074
>ref|NP_565610.1| expressed protein; protein id: At2g25830.1, supported by cDNA:
gi_15810382, supported by cDNA: gi_21281202 [Arabidopsis
thaliana] gi|22096384|sp|O82314|YB30_ARATH Hypothetical
protein At2g25830 gi|20197365|gb|AAC42247.2| expressed
protein [Arabidopsis thaliana]
Length = 331
Score = 134 bits (338), Expect(2) = 4e-31
Identities = 63/83 (75%), Positives = 75/83 (89%)
Frame = -3
Query: 629 GAEDVIDPPTYEEDTEEDESERYYKIVGSSENYSSILSKLREEGIDFVPDSGSELLPNAT 450
GAEDVI+PPTYE+DT+ED ERYYKIV S+ENYS+ILSKLR+EG++F PD+GSELLP T
Sbjct: 240 GAEDVIEPPTYEDDTDEDREERYYKIVTSNENYSTILSKLRDEGVNFEPDNGSELLPLTT 299
Query: 449 IEVDDEAMDLNKELMSNLLELDE 381
+EVDDEAM+LNKELM LLELD+
Sbjct: 300 VEVDDEAMELNKELMQKLLELDD 322
Score = 21.9 bits (45), Expect(2) = 4e-31
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1
Query: 394 LNLMSVDAVYTDQK 353
L L VDAVY DQK
Sbjct: 318 LELDDVDAVYIDQK 331
>gb|AAL07079.1| unknown protein [Arabidopsis thaliana] gi|21281203|gb|AAM45134.1|
unknown protein [Arabidopsis thaliana]
Length = 330
Score = 134 bits (338), Expect(2) = 4e-31
Identities = 63/83 (75%), Positives = 75/83 (89%)
Frame = -3
Query: 629 GAEDVIDPPTYEEDTEEDESERYYKIVGSSENYSSILSKLREEGIDFVPDSGSELLPNAT 450
GAEDVI+PPTYE+DT+ED ERYYKIV S+ENYS+ILSKLR+EG++F PD+GSELLP T
Sbjct: 239 GAEDVIEPPTYEDDTDEDREERYYKIVTSNENYSTILSKLRDEGVNFEPDNGSELLPLTT 298
Query: 449 IEVDDEAMDLNKELMSNLLELDE 381
+EVDDEAM+LNKELM LLELD+
Sbjct: 299 VEVDDEAMELNKELMQKLLELDD 321
Score = 21.9 bits (45), Expect(2) = 4e-31
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1
Query: 394 LNLMSVDAVYTDQK 353
L L VDAVY DQK
Sbjct: 317 LELDDVDAVYIDQK 330
>pir||C84653 hypothetical protein At2g25830 [imported] - Arabidopsis thaliana
Length = 339
Score = 127 bits (319), Expect(2) = 6e-29
Identities = 63/91 (69%), Positives = 75/91 (82%), Gaps = 8/91 (8%)
Frame = -3
Query: 629 GAEDVIDPPTYEEDTEEDESE--------RYYKIVGSSENYSSILSKLREEGIDFVPDSG 474
GAEDVI+PPTYE+DT+ED E RYYKIV S+ENYS+ILSKLR+EG++F PD+G
Sbjct: 240 GAEDVIEPPTYEDDTDEDREESFLSLYLFRYYKIVTSNENYSTILSKLRDEGVNFEPDNG 299
Query: 473 SELLPNATIEVDDEAMDLNKELMSNLLELDE 381
SELLP T+EVDDEAM+LNKELM LLELD+
Sbjct: 300 SELLPLTTVEVDDEAMELNKELMQKLLELDD 330
Score = 21.9 bits (45), Expect(2) = 6e-29
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1
Query: 394 LNLMSVDAVYTDQK 353
L L VDAVY DQK
Sbjct: 326 LELDDVDAVYIDQK 339
>ref|NP_013018.1| Suppressor of mutant AC40 subunit of RNA polymerase I and III (high
serine); Srp40p [Saccharomyces cerevisiae]
gi|548976|sp|P32583|SR40_YEAST SUPPRESSOR PROTEIN SRP40
gi|539364|pir||S38170 SRP40 protein - yeast
(Saccharomyces cerevisiae) gi|450552|emb|CAA51946.1| ORF
YKR412 [Saccharomyces cerevisiae]
gi|486581|emb|CAA82171.1| ORF YKR092c [Saccharomyces
cerevisiae]
Length = 406
Score = 38.5 bits (88), Expect = 0.074
Identities = 34/91 (37%), Positives = 47/91 (51%)
Frame = +1
Query: 325 KLLKIKKMTISGLYKQHQRSSSSSKLLISSLFKSIASSSTSIVAFGRSSEPLSGTKSMPS 504
K+ ++ K+++ K+ + SSSS SS S +SSS+S + G SS S + S S
Sbjct: 7 KVDEVPKLSVKE--KEIEEKSSSSSSSSSSSSSSSSSSSSSSSSSGESSSSSSSSSSSSS 64
Query: 505 SRSFDSIDE*FSEEPTIL*YLSDSSSSVSSS 597
S S DS D S + S SSSS SSS
Sbjct: 65 SDSSDSSDSESSSSSS-----SSSSSSSSSS 90
Score = 33.1 bits (74), Expect = 3.1
Identities = 32/72 (44%), Positives = 36/72 (49%)
Frame = +1
Query: 382 SSSSSKLLISSLFKSIASSSTSIVAFGRSSEPLSGTKSMPSSRSFDSIDE*FSEEPTIL* 561
SSSSS SS S +SSS+S + SE S + S SS S S E SE
Sbjct: 44 SSSSSSGESSSSSSSSSSSSSSDSSDSSDSESSSSSSSSSSSSSSSSDSESSSE------ 97
Query: 562 YLSDSSSSVSSS 597
SDSSSS SSS
Sbjct: 98 --SDSSSSGSSS 107
Score = 32.3 bits (72), Expect = 5.3
Identities = 36/117 (30%), Positives = 54/117 (45%)
Frame = +1
Query: 277 SYELPVAKNKR*AVYWKLLKIKKMTISGLYKQHQRSSSSSKLLISSLFKSIASSSTSIVA 456
S ++ V + + +V K ++ K + S SSSSS SS +S +SSS+S +
Sbjct: 3 SKKIKVDEVPKLSVKEKEIEEKSSSSSSSSSSSSSSSSSSSSSSSSSGESSSSSSSSSSS 62
Query: 457 FGRSSEPLSGTKSMPSSRSFDSIDE*FSEEPTIL*YLSDSSSSVSSS*VGGSITSSA 627
S S ++S SS S S S+ S+SSS SS G S +SS+
Sbjct: 63 SSSDSSDSSDSESSSSSSSSSSSSSSSSD--------SESSSESDSSSSGSSSSSSS 111
>gb|AAA35091.1| selected as a weak suppressor of a mutant of the subunit AC40 of
DNA dependant RNA polymerase I and III
Length = 406
Score = 38.5 bits (88), Expect = 0.074
Identities = 34/91 (37%), Positives = 47/91 (51%)
Frame = +1
Query: 325 KLLKIKKMTISGLYKQHQRSSSSSKLLISSLFKSIASSSTSIVAFGRSSEPLSGTKSMPS 504
K+ ++ K+++ K+ + SSSS SS S +SSS+S + G SS S + S S
Sbjct: 7 KVDEVPKLSVKE--KEIEEKSSSSSSSSSSSSSSSSSSSSSSSSSGESSSSSSSSSSSSS 64
Query: 505 SRSFDSIDE*FSEEPTIL*YLSDSSSSVSSS 597
S S DS D S + S SSSS SSS
Sbjct: 65 SDSSDSSDSESSSSSS-----SSSSSSSSSS 90
Score = 33.1 bits (74), Expect = 3.1
Identities = 32/72 (44%), Positives = 36/72 (49%)
Frame = +1
Query: 382 SSSSSKLLISSLFKSIASSSTSIVAFGRSSEPLSGTKSMPSSRSFDSIDE*FSEEPTIL* 561
SSSSS SS S +SSS+S + SE S + S SS S S E SE
Sbjct: 44 SSSSSSGESSSSSSSSSSSSSSDSSDSSDSESSSSSSSSSSSSSSSSDSESSSE------ 97
Query: 562 YLSDSSSSVSSS 597
SDSSSS SSS
Sbjct: 98 --SDSSSSGSSS 107
Score = 32.3 bits (72), Expect = 5.3
Identities = 36/117 (30%), Positives = 54/117 (45%)
Frame = +1
Query: 277 SYELPVAKNKR*AVYWKLLKIKKMTISGLYKQHQRSSSSSKLLISSLFKSIASSSTSIVA 456
S ++ V + + +V K ++ K + S SSSSS SS +S +SSS+S +
Sbjct: 3 SKKIKVDEVPKLSVKEKEIEEKSSSSSSSSSSSSSSSSSSSSSSSSSGESSSSSSSSSSS 62
Query: 457 FGRSSEPLSGTKSMPSSRSFDSIDE*FSEEPTIL*YLSDSSSSVSSS*VGGSITSSA 627
S S ++S SS S S S+ S+SSS SS G S +SS+
Sbjct: 63 SSSDSSDSSDSESSSSSSSSSSSSSSSSD--------SESSSESDSSSSGSSSSSSS 111
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 495,964,201
Number of Sequences: 1393205
Number of extensions: 9886272
Number of successful extensions: 29031
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 24763
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27074
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25870486187
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
