>KMC003495A_C01 KMC003495A_c01
tgggtacgggcccccctcagttctttctttttttattaacgtatgtagaaaaatatccaa
tactaataattgcgtaactgAAGAGCGAGGACAAAGCGTGACACAGAACAGATGATTACA
AATCAAATTAAAAGGAAACGAAAGGGTGGGATGATTTTCCCATGCATTATTGTTGCTGGT
TTTCTAGCATTCTAAAACAAATGAAATTGCTACTGTTTTGAATGTTGACACAAGTGGAAA
CGGACGTAAGTACTCACTTCAAAACTAAAACTAAATGAAACTCTTATTCGGATTGAAATG
AAAATCCAGTAGGACTTAGGACTCAACTGTAATCCAAACACACACAAAGTCTACCTATAC
CATTGATAATCTCTCCATGGATGCATCATAGTATTTGACACTTCCACATCACATGACTTT
CATGGTCATTTGTAAGGGGATACCAACTGAGCAATTTTATGCAATAGCTTGGATGCATCT
TGACTCTTATAGGAGGTCCACTCTTGAAAATTATGAAGACTACCAGTGAAGTCTGTGAAA
AGGCCATCAACTCCTACCTTGTTGATCCAGTAATCATACTCCACATACGGATCTTGGCTA
AAATTGAAATGCAAATACTTGTTCTCATTTCTGTAAGTATATGGATGCACCTGCAGGTTA
TGAGCATGTGCCCTGGCAACAAGATCAGTGG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003495A_C01 KMC003495A_c01
         (691 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_177561.1| putative glycerophosphodiester phosphodiesteras...   145  7e-34
dbj|BAB92381.1| putative glycerophosphoryl diester phosphodieste...   137  1e-31
ref|NP_196420.1| glycerophosphodiester phosphodiesterase - like ...   125  7e-28
ref|NP_484319.1| glycerophosphoryl diester phosphodiesterase [No...    57  2e-07
gb|AAB49412.1| glycerophosphodiester phosphodiesterase gi|151321...    54  3e-06

>ref|NP_177561.1| putative glycerophosphodiester phosphodiesterase; protein id:
           At1g74210.1, supported by cDNA: 24182., supported by
           cDNA: gi_17381161 [Arabidopsis thaliana]
           gi|25406323|pir||D96770 hypothetical protein F1O17.12
           [imported] - Arabidopsis thaliana
           gi|12324904|gb|AAG52406.1|AC020579_8 putative
           glycerophosphodiester phosphodiesterase; 42559-40170
           [Arabidopsis thaliana] gi|17381162|gb|AAL36393.1|
           putative glycerophosphodiester phosphodiesterase
           [Arabidopsis thaliana] gi|21592460|gb|AAM64411.1|
           putative glycerophosphodiester phosphodiesterase
           [Arabidopsis thaliana] gi|23296874|gb|AAN13192.1|
           putative glycerophosphodiester phosphodiesterase
           [Arabidopsis thaliana]
          Length = 392

 Score =  145 bits (365), Expect = 7e-34
 Identities = 67/89 (75%), Positives = 76/89 (85%), Gaps = 3/89 (3%)
 Frame = -3

Query: 689 TDLVARAHAHNLQVHPYTYRNENKYLHFNFSQDPYVEYDYWINKVGVDGLFTDFTGSLHN 510
           TDLV RAHAHNLQVHPYTYRNE+++LH+NFSQDPY EYDYWIN++GVDGLFTDFTGSLHN
Sbjct: 301 TDLVKRAHAHNLQVHPYTYRNEHEFLHYNFSQDPYKEYDYWINEIGVDGLFTDFTGSLHN 360

Query: 509 FQEWTS---YKSQDASKLLHKIAQLVSPY 432
           FQEWTS     S+   +LL +IA LV PY
Sbjct: 361 FQEWTSPLPDTSKSPRQLLSQIASLVLPY 389

>dbj|BAB92381.1| putative glycerophosphoryl diester phosphodiesterase [Oryza sativa
           (japonica cultivar-group)]
          Length = 391

 Score =  137 bits (345), Expect = 1e-31
 Identities = 63/89 (70%), Positives = 74/89 (82%), Gaps = 2/89 (2%)
 Frame = -3

Query: 689 TDLVARAHAHNLQVHPYTYRNENKYLHFNFSQDPYVEYDYWINKVGVDGLFTDFTGSLHN 510
           TDLVARAHAHNLQVHPYTYRNEN++LH NF QDPY EYD+WIN +GVDGLFTDFTGSLH 
Sbjct: 301 TDLVARAHAHNLQVHPYTYRNENQFLHLNFHQDPYAEYDFWINSMGVDGLFTDFTGSLHR 360

Query: 509 FQEWTS--YKSQDASKLLHKIAQLVSPYK 429
           +QE  +   K + A+ LL KIAQ++S Y+
Sbjct: 361 YQELVAPHAKDETANSLLVKIAQMISQYE 389

>ref|NP_196420.1| glycerophosphodiester phosphodiesterase - like protein; protein id:
           At5g08030.1 [Arabidopsis thaliana]
           gi|11357308|pir||T45628 glycerophosphodiester
           phosphodiesterase-like protein - Arabidopsis thaliana
           gi|6562317|emb|CAB62615.1| glycerophosphodiester
           phosphodiesterase-like protein [Arabidopsis thaliana]
          Length = 372

 Score =  125 bits (313), Expect = 7e-28
 Identities = 53/66 (80%), Positives = 61/66 (92%)
 Frame = -3

Query: 689 TDLVARAHAHNLQVHPYTYRNENKYLHFNFSQDPYVEYDYWINKVGVDGLFTDFTGSLHN 510
           TDLVARAH+ NLQVHPYTYRNEN++LH  F+QDPY+EYDYW+NK+GVDGLFTDFTGSLHN
Sbjct: 301 TDLVARAHSRNLQVHPYTYRNENQFLHLEFNQDPYLEYDYWLNKIGVDGLFTDFTGSLHN 360

Query: 509 FQEWTS 492
           +QE  S
Sbjct: 361 YQELKS 366

>ref|NP_484319.1| glycerophosphoryl diester phosphodiesterase [Nostoc sp. PCC 7120]
           gi|25530449|pir||AC1841 glycerophosphoryl diester
           phosphodiesterase [imported] - Nostoc sp. (strain PCC
           7120) gi|17135253|dbj|BAB77799.1| glycerophosphoryl
           diester phosphodiesterase [Nostoc sp. PCC 7120]
          Length = 1027

 Score = 57.0 bits (136), Expect = 2e-07
 Identities = 28/54 (51%), Positives = 37/54 (67%)
 Frame = -3

Query: 689 TDLVARAHAHNLQVHPYTYRNENKYLHFNFSQDPYVEYDYWINKVGVDGLFTDF 528
           T LV  AH   LQVHPYT+R+E +YL  N+  +P +EY   + ++GVD LFTDF
Sbjct: 490 TTLVQDAHNVGLQVHPYTFRDEERYLAANYQGNPELEYQQ-LFQLGVDALFTDF 542

>gb|AAB49412.1| glycerophosphodiester phosphodiesterase gi|1513213|gb|AAB51468.1|
           38 kDa lipoprotein Gpd [Borrelia hermsii]
          Length = 340

 Score = 53.5 bits (127), Expect = 3e-06
 Identities = 27/61 (44%), Positives = 38/61 (62%)
 Frame = -3

Query: 689 TDLVARAHAHNLQVHPYTYRNENKYLHFNFSQDPYVEYDYWINKVGVDGLFTDFTGSLHN 510
           TDL + AH HN++VHPYT+R +      ++ +D     D   NK  VDG+FTDFT ++ N
Sbjct: 277 TDLTSLAHKHNMEVHPYTFRIDALP---SYVKDADELLDLLFNKAKVDGIFTDFTDTVVN 333

Query: 509 F 507
           F
Sbjct: 334 F 334

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 586,638,205
Number of Sequences: 1393205
Number of extensions: 11758595
Number of successful extensions: 24444
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 23712
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24401
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31118763720
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD064a07_f AV774243 1 481 2 GNf052b06 BP071210 100 504 3 MF008d02_f BP028635 100 593 4 MWM230e02_f AV768237 114 464 5 MR096b06_f BP083340 130 617 6 MF036b09_f BP030191 130 574 7 GNf046a11 BP070734 131 550 8 SPD100b10_f BP051961 138 692 9 MPD058c06_f AV773875 145 595 10 MF003d01_f BP028386 150 648 11 SPDL005g05_f BP052307 175 649

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