Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003455A_C02 KMC003455A_c02
(1330 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_176633.1| 26S proteasome regulatory subunit (RPN12), puta... 453 e-126
dbj|BAB78490.1| 26S proteasome regulatory particle non-ATPase su... 446 e-124
ref|NP_199019.1| putative protein; protein id: At5g42040.1 [Arab... 267 2e-70
gb|AAO51336.1| similar to Oryza sativa (Rice). 26S proteasome re... 193 6e-48
ref|NP_648904.1| CG4157-PA [Drosophila melanogaster] gi|6434966|... 174 2e-42
>ref|NP_176633.1| 26S proteasome regulatory subunit (RPN12), putative; protein id:
At1g64520.1, supported by cDNA: gi_14532465, supported by
cDNA: gi_15294147 [Arabidopsis thaliana]
gi|25338454|pir||H96668 protein F1N19.9 [imported] -
Arabidopsis thaliana gi|6633812|gb|AAF19671.1|AC009519_5
F1N19.9 [Arabidopsis thaliana] gi|14532466|gb|AAK63961.1|
At1g64520/F1N19_10 [Arabidopsis thaliana]
gi|15294148|gb|AAK95251.1|AF410265_1 At1g64520/F1N19_10
[Arabidopsis thaliana] gi|23505935|gb|AAN28827.1|
At1g64520/F1N19_10 [Arabidopsis thaliana]
Length = 267
Score = 453 bits (1165), Expect = e-126
Identities = 228/267 (85%), Positives = 243/267 (90%)
Frame = -2
Query: 1233 MDPKLTEVSQIFDRFKAAFRRNDFDNCSDLLSQLKVLLTGFRSLPPLFENTPNAVHELTI 1054
MDP+LTEVSQ F+RFKAAF R D++ CSDLLSQLKVLLT F SLPPLFEN+PNA ELTI
Sbjct: 1 MDPQLTEVSQQFERFKAAFARKDYNTCSDLLSQLKVLLTKFTSLPPLFENSPNAAKELTI 60
Query: 1053 ARDIYEHAVVLSVKTEDQDAFERDFFQLKPYYTDASNRISPSPQEYPILGLNLLRLLVQN 874
ARDIYEHAVVLSVKTEDQDAFERDFFQLKPYY DA NRI SPQE ILGLNLLRLLVQN
Sbjct: 61 ARDIYEHAVVLSVKTEDQDAFERDFFQLKPYYVDARNRIPQSPQENLILGLNLLRLLVQN 120
Query: 873 RIAEFHTELELLSSAALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHDTYVYFMDLLA 694
RIAEFHTELELLSSA LE+PCIKHAVELEQSFMEGAYNRVLSARQT P TYVYFMDLLA
Sbjct: 121 RIAEFHTELELLSSATLEDPCIKHAVELEQSFMEGAYNRVLSARQTAPDATYVYFMDLLA 180
Query: 693 KTVRDEIAGCSEKAYDYLSIKDARQILLYSSDQELLEYIKEEHPEWEIKNGSVFFQKAKE 514
KT+RDEIAGCSEKAYDY+SI DARQ+LL+SSDQELL Y+ +EHPEWE+K G V FQKAKE
Sbjct: 181 KTIRDEIAGCSEKAYDYVSISDARQMLLFSSDQELLTYVTDEHPEWEVKEGFVVFQKAKE 240
Query: 513 SAPCQEIPSLQLINQTLSYARELERIV 433
+APC+EIPSLQLINQTLSYARELERIV
Sbjct: 241 TAPCKEIPSLQLINQTLSYARELERIV 267
>dbj|BAB78490.1| 26S proteasome regulatory particle non-ATPase subunit12 [Oryza sativa
(japonica cultivar-group)]
Length = 267
Score = 446 bits (1146), Expect = e-124
Identities = 221/267 (82%), Positives = 238/267 (88%)
Frame = -2
Query: 1233 MDPKLTEVSQIFDRFKAAFRRNDFDNCSDLLSQLKVLLTGFRSLPPLFENTPNAVHELTI 1054
MDPKL EV+Q+F RFKAAF RNDFD C DLLSQLKV LT F SLPP F+ TPNAV EL I
Sbjct: 1 MDPKLVEVTQLFSRFKAAFARNDFDTCVDLLSQLKVRLTKFPSLPPSFQQTPNAVEELKI 60
Query: 1053 ARDIYEHAVVLSVKTEDQDAFERDFFQLKPYYTDASNRISPSPQEYPILGLNLLRLLVQN 874
ARDIYEHAVVLSVK EDQDAFERDF QLKPYY D I PSPQEYPILGLNLLRLLVQN
Sbjct: 61 ARDIYEHAVVLSVKIEDQDAFERDFCQLKPYYMDTCGIIPPSPQEYPILGLNLLRLLVQN 120
Query: 873 RIAEFHTELELLSSAALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHDTYVYFMDLLA 694
RIAEFHTELELL ALENPCIKHAVELEQSFMEGAYNRVLSARQ VPH+TYVYFMDLLA
Sbjct: 121 RIAEFHTELELLPVTALENPCIKHAVELEQSFMEGAYNRVLSARQAVPHETYVYFMDLLA 180
Query: 693 KTVRDEIAGCSEKAYDYLSIKDARQILLYSSDQELLEYIKEEHPEWEIKNGSVFFQKAKE 514
KTVRDE+AGCSEK YDY+SI +ARQ+L++SSD+EL +YI EEHPEWE+K+GSVFFQKAKE
Sbjct: 181 KTVRDELAGCSEKGYDYMSIAEARQVLMFSSDKELHQYIAEEHPEWEVKDGSVFFQKAKE 240
Query: 513 SAPCQEIPSLQLINQTLSYARELERIV 433
+ PC+EIPSLQ+INQTLSYARELERIV
Sbjct: 241 TQPCKEIPSLQVINQTLSYARELERIV 267
>ref|NP_199019.1| putative protein; protein id: At5g42040.1 [Arabidopsis thaliana]
gi|9757949|dbj|BAB08437.1| contains similarity to 26S
proteasome regulatory complex subunit~gene_id:MJC20.14
[Arabidopsis thaliana]
Length = 159
Score = 267 bits (683), Expect = 2e-70
Identities = 130/159 (81%), Positives = 146/159 (91%)
Frame = -2
Query: 909 LGLNLLRLLVQNRIAEFHTELELLSSAALENPCIKHAVELEQSFMEGAYNRVLSARQTVP 730
+GLNLLRLLVQNRIAEFHTEL LLSSA LENPCIKHAVELEQSFMEGAYNRVLSARQT P
Sbjct: 1 MGLNLLRLLVQNRIAEFHTELGLLSSATLENPCIKHAVELEQSFMEGAYNRVLSARQTAP 60
Query: 729 HDTYVYFMDLLAKTVRDEIAGCSEKAYDYLSIKDARQILLYSSDQELLEYIKEEHPEWEI 550
+TYVYFMDLLAKT+RDEIAGCSEKAYD+LSI + ++LL+SSDQ+LL Y+ EEHPEWE+
Sbjct: 61 DETYVYFMDLLAKTIRDEIAGCSEKAYDHLSISEGCKMLLFSSDQQLLTYVNEEHPEWEV 120
Query: 549 KNGSVFFQKAKESAPCQEIPSLQLINQTLSYARELERIV 433
K+G V FQK +E+APC+EIPSLQLINQTLSY RELERI+
Sbjct: 121 KDGLVVFQKTRETAPCKEIPSLQLINQTLSYTRELERIL 159
>gb|AAO51336.1| similar to Oryza sativa (Rice). 26S proteasome regulatory particle
non-ATPase subunit12 [Dictyostelium discoideum]
Length = 263
Score = 193 bits (490), Expect = 6e-48
Identities = 107/264 (40%), Positives = 166/264 (62%), Gaps = 2/264 (0%)
Frame = -2
Query: 1218 TEVSQIFDRFKAAFRRN-DFDNCSDLLSQLKVLLTGFRSLPPLFENTPNAVH-ELTIARD 1045
+ + Q + FK N D + +L QLK+ T P N + V +L +AR+
Sbjct: 4 SSIEQNLNNFKKLVAGNSDKAQITPVLVQLKLAATIHLEKPTSLSNVSDKVKKDLVLARE 63
Query: 1044 IYEHAVVLSVKTEDQDAFERDFFQLKPYYTDASNRISPSPQEYPILGLNLLRLLVQNRIA 865
I E + S+K +D D+FER F QLK YY D + I+PS EY I+GLNL+RLL +++ +
Sbjct: 64 ILELISLYSIKIKDIDSFERTFNQLKTYYYDYKSIIAPSTLEYQIIGLNLMRLLAKHKTS 123
Query: 864 EFHTELELLSSAALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHDTYVYFMDLLAKTV 685
EFH+E+EL+ L+N IK + +E+S EG+YN+++ +R VP + Y F+D+LA ++
Sbjct: 124 EFHSEIELIEFNNLDNSFIKFPLLVEKSITEGSYNKIIQSRSGVPSEYYQVFLDILADSI 183
Query: 684 RDEIAGCSEKAYDYLSIKDARQILLYSSDQELLEYIKEEHPEWEIKNGSVFFQKAKESAP 505
+++IA CSEK++ LS+KDA ++LL++ + + +YIKE + W+++ + F
Sbjct: 184 KEDIANCSEKSFKTLSLKDAEKVLLFNDNNQFQQYIKERN--WKVQGDVIQF--GNNDNQ 239
Query: 504 CQEIPSLQLINQTLSYARELERIV 433
EIPSLQLI+QTL YA+ELERIV
Sbjct: 240 TVEIPSLQLIHQTLHYAKELERIV 263
>ref|NP_648904.1| CG4157-PA [Drosophila melanogaster]
gi|6434966|gb|AAF08395.1|AF145314_1 26S proteasome
regulatory complex subunit p30 [Drosophila melanogaster]
gi|7294091|gb|AAF49445.1| CG4157-PA [Drosophila
melanogaster] gi|19527567|gb|AAL89898.1| RE36854p
[Drosophila melanogaster]
Length = 264
Score = 174 bits (442), Expect = 2e-42
Identities = 104/242 (42%), Positives = 143/242 (58%), Gaps = 1/242 (0%)
Frame = -2
Query: 1155 CSDLLSQLKVLLTGFRSLPPLFENTPNAVHELTIARDIYEHAVVLSVKTEDQDAFERDFF 976
C LL QLKV L LP + ++ +L +AR + E AV SV ++D AFER
Sbjct: 25 CGQLLDQLKVALVKMAFLPTDGNDAQSSKKQLILARSVLEVAVEHSVLSKDLLAFERYMA 84
Query: 975 QLKPYYTDASNRISPSPQEYPILGLNLLRLLVQNRIAEFHTELELLSSAALE-NPCIKHA 799
QLK YY D + I S +Y +LGLNLL LL NR+++FHTELELLS ++ N I+
Sbjct: 85 QLKCYYYDYAKIIGESESKYKLLGLNLLYLLSGNRVSDFHTELELLSVDVIQHNQFIRPI 144
Query: 798 VELEQSFMEGAYNRVLSARQTVPHDTYVYFMDLLAKTVRDEIAGCSEKAYDYLSIKDARQ 619
+ LEQ MEG YN++ A+ TVP + Y YFMDLL +TVRDEI C EK+YD +S KDA +
Sbjct: 145 LALEQYIMEGRYNKIFQAKSTVPVEVYSYFMDLLLETVRDEIGACIEKSYDKISAKDAAK 204
Query: 618 ILLYSSDQELLEYIKEEHPEWEIKNGSVFFQKAKESAPCQEIPSLQLINQTLSYARELER 439
L E+ + + +W+++ + + P + +PS +L Q LSYAR+LE
Sbjct: 205 RLNLRVPDEIKAFGAKR--QWKLEASGDYSFTDRSVKPKELLPSEELAEQVLSYARDLEM 262
Query: 438 IV 433
IV
Sbjct: 263 IV 264
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,093,985,874
Number of Sequences: 1393205
Number of extensions: 24067198
Number of successful extensions: 59329
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 55928
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 59256
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 86313951772
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
