Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003435A_C01 KMC003435A_c01
(1321 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_565482.1| putative riboflavin synthase alpha chain; prote... 334 2e-90
gb|AAM65832.1| putative riboflavin synthase alpha chain [Arabido... 334 2e-90
pir||B84592 probable riboflavin synthase alpha chain [imported] ... 304 2e-81
gb|ZP_00018137.1| hypothetical protein [Chloroflexus aurantiacus] 198 1e-49
gb|AAL66353.1|AF459790_1 riboflavin synthase [Pichia guilliermon... 196 5e-49
>ref|NP_565482.1| putative riboflavin synthase alpha chain; protein id: At2g20690.1,
supported by cDNA: 528., supported by cDNA: gi_13605592,
supported by cDNA: gi_19548076 [Arabidopsis thaliana]
gi|13605593|gb|AAK32790.1|AF361622_1 At2g20690/F5H14.34
[Arabidopsis thaliana] gi|19548077|gb|AAL87403.1|
At2g20690/F5H14.34 [Arabidopsis thaliana]
gi|20197688|gb|AAD20926.2| putative riboflavin synthase
alpha chain [Arabidopsis thaliana]
Length = 271
Score = 334 bits (857), Expect = 2e-90
Identities = 171/244 (70%), Positives = 201/244 (82%)
Frame = -2
Query: 1218 RHHSTFTNLRFNPIAKPSPLRFFLPLTPQNPHHHRRRSAVVTNMFTGLVEELGTVKQLGM 1039
R + TNLRF+ ++K S L ++ HHRR++ + ++FTG+VEE+G VK LGM
Sbjct: 22 RTGESVTNLRFDCVSKSSKLSLKTSCG-RSRTHHRRQNLSIRSVFTGIVEEMGEVKDLGM 80
Query: 1038 ASHGGFDLNVEAKTVLEGVILGDSIAVNGTCLTVTEFDLEGSNFTVGLSPETLRKTSLEE 859
A HGGFDL + A+ VLE V LGDSIAVNGTCLTVTEF+ E FTVGL+PETLRKTSLEE
Sbjct: 81 ADHGGFDLKIGARVVLEDVKLGDSIAVNGTCLTVTEFNAE--EFTVGLAPETLRKTSLEE 138
Query: 858 LEPGSVVNLERALLPTTRMGGHFVQGHVDGTGVIVSMDAEGDSLWVKVKAEKEVLKYIVP 679
L+ GS VNLERAL P +RMGGH VQGHVDGTGVI SM+ EGDSLWVKVKA+K +LKYIVP
Sbjct: 139 LKKGSPVNLERALQPVSRMGGHVVQGHVDGTGVIESMEVEGDSLWVKVKADKGLLKYIVP 198
Query: 678 KGFIAVDGTSLTVVDVFDDEGCFNFMLVAYTQQKVVIPLKKVGRKVNLEVDILGKYVERL 499
KGF+AVDGTSLTVVDVFD+E CFNFM++AYTQQ VVIP KK+G+KVNLEVDI+GKYVERL
Sbjct: 199 KGFVAVDGTSLTVVDVFDEESCFNFMMIAYTQQNVVIPTKKIGQKVNLEVDIMGKYVERL 258
Query: 498 LSSG 487
L+SG
Sbjct: 259 LTSG 262
>gb|AAM65832.1| putative riboflavin synthase alpha chain [Arabidopsis thaliana]
Length = 270
Score = 334 bits (856), Expect = 2e-90
Identities = 171/244 (70%), Positives = 201/244 (82%)
Frame = -2
Query: 1218 RHHSTFTNLRFNPIAKPSPLRFFLPLTPQNPHHHRRRSAVVTNMFTGLVEELGTVKQLGM 1039
R + TNLRF+ ++K S L ++ HHRR++ + ++FTG+VEE+G VK LGM
Sbjct: 21 RTGESVTNLRFDCVSKSSKLSLKTSCG-RSRTHHRRQNLSIRSVFTGIVEEMGEVKDLGM 79
Query: 1038 ASHGGFDLNVEAKTVLEGVILGDSIAVNGTCLTVTEFDLEGSNFTVGLSPETLRKTSLEE 859
A HGGFDL + AK VL+ V LGDSIAVNGTCLTVTEF+ E FTVGL+PETLRKTSLEE
Sbjct: 80 ADHGGFDLKIGAKVVLKDVKLGDSIAVNGTCLTVTEFNAE--EFTVGLAPETLRKTSLEE 137
Query: 858 LEPGSVVNLERALLPTTRMGGHFVQGHVDGTGVIVSMDAEGDSLWVKVKAEKEVLKYIVP 679
L+ GS VNLERAL P +RMGGH VQGHVDGTGVI SM+ EGDSLWVKVKA+K +LKYIVP
Sbjct: 138 LKKGSPVNLERALQPVSRMGGHVVQGHVDGTGVIESMEVEGDSLWVKVKADKGLLKYIVP 197
Query: 678 KGFIAVDGTSLTVVDVFDDEGCFNFMLVAYTQQKVVIPLKKVGRKVNLEVDILGKYVERL 499
KGF+AVDGTSLTVVDVFD+E CFNFM++AYTQQ VVIP KK+G+KVNLEVDI+GKYVERL
Sbjct: 198 KGFVAVDGTSLTVVDVFDEESCFNFMMIAYTQQNVVIPTKKIGQKVNLEVDIMGKYVERL 257
Query: 498 LSSG 487
L+SG
Sbjct: 258 LTSG 261
>pir||B84592 probable riboflavin synthase alpha chain [imported] - Arabidopsis
thaliana
Length = 197
Score = 304 bits (778), Expect = 2e-81
Identities = 152/193 (78%), Positives = 170/193 (87%)
Frame = -2
Query: 1065 LGTVKQLGMASHGGFDLNVEAKTVLEGVILGDSIAVNGTCLTVTEFDLEGSNFTVGLSPE 886
+G VK LGMA HGGFDL + A+ VLE V LGDSIAVNGTCLTVTEF+ E FTVGL+PE
Sbjct: 1 MGEVKDLGMADHGGFDLKIGARVVLEDVKLGDSIAVNGTCLTVTEFNAE--EFTVGLAPE 58
Query: 885 TLRKTSLEELEPGSVVNLERALLPTTRMGGHFVQGHVDGTGVIVSMDAEGDSLWVKVKAE 706
TLRKTSLEEL+ GS VNLERAL P +RMGGH VQGHVDGTGVI SM+ EGDSLWVKVKA+
Sbjct: 59 TLRKTSLEELKKGSPVNLERALQPVSRMGGHVVQGHVDGTGVIESMEVEGDSLWVKVKAD 118
Query: 705 KEVLKYIVPKGFIAVDGTSLTVVDVFDDEGCFNFMLVAYTQQKVVIPLKKVGRKVNLEVD 526
K +LKYIVPKGF+AVDGTSLTVVDVFD+E CFNFM++AYTQQ VVIP KK+G+KVNLEVD
Sbjct: 119 KGLLKYIVPKGFVAVDGTSLTVVDVFDEESCFNFMMIAYTQQNVVIPTKKIGQKVNLEVD 178
Query: 525 ILGKYVERLLSSG 487
I+GKYVERLL+SG
Sbjct: 179 IMGKYVERLLTSG 191
>gb|ZP_00018137.1| hypothetical protein [Chloroflexus aurantiacus]
Length = 213
Score = 198 bits (504), Expect = 1e-49
Identities = 110/200 (55%), Positives = 135/200 (67%)
Frame = -2
Query: 1089 MFTGLVEELGTVKQLGMASHGGFDLNVEAKTVLEGVILGDSIAVNGTCLTVTEFDLEGSN 910
MFTG+VEE+GTV + + + +TVLEG +GDSIAVNGTCLTVT L +
Sbjct: 12 MFTGIVEEMGTVAHIVSTQEQANQITITCRTVLEGTRIGDSIAVNGTCLTVTA--LTADS 69
Query: 909 FTVGLSPETLRKTSLEELEPGSVVNLERALLPTTRMGGHFVQGHVDGTGVIVSMDAEGDS 730
FTVGLSPETLR+T+L L G VNLERAL RMGGH+VQGHVDG G IV++ EGDS
Sbjct: 70 FTVGLSPETLRRTNLGRLHVGDPVNLERALAFGGRMGGHYVQGHVDGVGEIVAIVPEGDS 129
Query: 729 LWVKVKAEKEVLKYIVPKGFIAVDGTSLTVVDVFDDEGCFNFMLVAYTQQKVVIPLKKVG 550
V ++ +L YIV KG+IAVDG SLTV ++ F LVAYTQ V++ + VG
Sbjct: 130 KRVTIRPPAALLPYIVEKGYIAVDGVSLTVAEM--GRETFTVALVAYTQSAVIMGRQTVG 187
Query: 549 RKVNLEVDILGKYVERLLSS 490
VN+EVDI+ KYVERLL +
Sbjct: 188 ALVNIEVDIIAKYVERLLEA 207
>gb|AAL66353.1|AF459790_1 riboflavin synthase [Pichia guilliermondii]
Length = 239
Score = 196 bits (499), Expect = 5e-49
Identities = 107/218 (49%), Positives = 145/218 (66%), Gaps = 7/218 (3%)
Frame = -2
Query: 1089 MFTGLVEELGTVKQL---GMASHGGFDLNV---EAKTVLEGVILGDSIAVNGTCLTVTEF 928
MFTGLVE +GTV Q+ + GG +++ + +LE V LGDSI NG CLTVTEF
Sbjct: 1 MFTGLVEHIGTVLQVTEKDTTASGGDGVSMVIGDCLKILEDVQLGDSICTNGVCLTVTEF 60
Query: 927 DLEGSNFTVGLSPETLRKTSLEELEPGSVVNLERALLPTTRMGGHFVQGHVDGTGVIVSM 748
D+ S F VG+SPETLR++ L EL+PGS VNLERA+ RMGGH VQGHVD IV+
Sbjct: 61 DMARSQFKVGISPETLRRSDLGELKPGSKVNLERAVKADVRMGGHVVQGHVDTIATIVNR 120
Query: 747 DAEGDSLWVKVK-AEKEVLKYIVPKGFIAVDGTSLTVVDVFDDEGCFNFMLVAYTQQKVV 571
+G+++ K + + KYIV KGFIA+DGTSLTV DV ++ F+ ++V+YTQ+KV+
Sbjct: 121 RGDGNAINFTFKLRDSQYGKYIVEKGFIAIDGTSLTVTDVDHEQSEFSILMVSYTQEKVI 180
Query: 570 IPLKKVGRKVNLEVDILGKYVERLLSSGFVQSIANTNS 457
+PLK G VN+EVD+ GK +E+ + + I + S
Sbjct: 181 MPLKNSGDSVNIEVDLTGKLIEKQIELSLLSYIKDETS 218
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,251,852,212
Number of Sequences: 1393205
Number of extensions: 33538062
Number of successful extensions: 235833
Number of sequences better than 10.0: 2219
Number of HSP's better than 10.0 without gapping: 125391
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199513
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 85494515521
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
