Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003362A_C01 KMC003362A_c01
(1065 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|O48551|PSA6_SOYBN Proteasome subunit alpha type 6 (20S protea... 393 e-126
sp|Q9XG77|PSA6_TOBAC Proteasome subunit alpha type 6 (20S protea... 385 e-121
ref|NP_198409.1| 20S proteasome alpha subunit A1 (PAA1); protein... 372 e-117
gb|AAM12968.1| multicatalytic endopeptidase complex alpha subuni... 370 e-117
emb|CAA74025.1| multicatalytic endopeptidase complex, proteasome... 370 e-117
>sp|O48551|PSA6_SOYBN Proteasome subunit alpha type 6 (20S proteasome alpha subunit A)
(20S proteasome subunit alpha-1) (Proteasome iota
subunit) gi|7435869|pir||T06142 C 3.4.25.1 proteasome
endopeptidase complex () iota chain - soybean
gi|3377794|gb|AAC28135.1| proteasome IOTA subunit
[Glycine max]
Length = 246
Score = 393 bits (1009), Expect(2) = e-126
Identities = 197/210 (93%), Positives = 199/210 (93%)
Frame = -1
Query: 921 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTQKKVPDKL 742
MSRGSGGGY+RHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVT KKVPDKL
Sbjct: 1 MSRGSGGGYNRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTHKKVPDKL 60
Query: 741 LDQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRHTYGYEMPVDVLAKWIAD 562
LD TSV+HLFPITKYLGLLATGMTADARTLVQQARNEAAEFR TYGYEMPVDVLAKWIAD
Sbjct: 61 LDNTSVTHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRFTYGYEMPVDVLAKWIAD 120
Query: 561 KSQVYTQHAYMRPLGVVAMVLGIDDENGAQLYKCDPAGHYFGHKATSAGLKDQEAINFLE 382
KSQVYTQHAYMRPLGVVAMVLGIDDE G QLYKCDPAGHYFGHKATSAGLKDQEAINFLE
Sbjct: 121 KSQVYTQHAYMRPLGVVAMVLGIDDEYGPQLYKCDPAGHYFGHKATSAGLKDQEAINFLE 180
Query: 381 KKMKNDPSFTYEETVQTAISALPSASSGGF 292
KKMKNDPSFTYEETVQTAISAL S F
Sbjct: 181 KKMKNDPSFTYEETVQTAISALQSVLQEDF 210
Score = 82.8 bits (203), Expect(2) = e-126
Identities = 40/42 (95%), Positives = 42/42 (99%)
Frame = -2
Query: 308 LLQEDFKATEIEVGVVRKDNPEFRVLSTDEIDEHLTAISERD 183
+LQEDFKATEIEVGVVRKDNPEFRVL+TDEIDEHLTAISERD
Sbjct: 205 VLQEDFKATEIEVGVVRKDNPEFRVLTTDEIDEHLTAISERD 246
>sp|Q9XG77|PSA6_TOBAC Proteasome subunit alpha type 6 (20S proteasome alpha subunit A)
(20S proteasome subunit alpha-1)
gi|4539545|emb|CAB39975.1| PRCI [Nicotiana tabacum]
Length = 246
Score = 385 bits (989), Expect(2) = e-121
Identities = 190/210 (90%), Positives = 199/210 (94%)
Frame = -1
Query: 921 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTQKKVPDKL 742
MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDS+CVVTQKKVPDKL
Sbjct: 1 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSVCVVTQKKVPDKL 60
Query: 741 LDQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRHTYGYEMPVDVLAKWIAD 562
LDQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFR YGYEMPVDVL+KWIAD
Sbjct: 61 LDQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRFKYGYEMPVDVLSKWIAD 120
Query: 561 KSQVYTQHAYMRPLGVVAMVLGIDDENGAQLYKCDPAGHYFGHKATSAGLKDQEAINFLE 382
KSQVYTQHAYMRPLGVVAM+LGID+E G QL+KCDPAGH+FGHKATSAG K+QEAINFLE
Sbjct: 121 KSQVYTQHAYMRPLGVVAMILGIDEEKGPQLFKCDPAGHFFGHKATSAGSKEQEAINFLE 180
Query: 381 KKMKNDPSFTYEETVQTAISALPSASSGGF 292
KKMKNDP+F+YEETVQTAISAL S F
Sbjct: 181 KKMKNDPAFSYEETVQTAISALQSVLQEDF 210
Score = 72.0 bits (175), Expect(2) = e-121
Identities = 34/42 (80%), Positives = 41/42 (96%)
Frame = -2
Query: 308 LLQEDFKATEIEVGVVRKDNPEFRVLSTDEIDEHLTAISERD 183
+LQEDFKA+EIEVGVV+K++P FRVL+T+EIDEHLTAISERD
Sbjct: 205 VLQEDFKASEIEVGVVKKEDPIFRVLTTEEIDEHLTAISERD 246
>ref|NP_198409.1| 20S proteasome alpha subunit A1 (PAA1); protein id: At5g35590.1,
supported by cDNA: gi_20260139, supported by cDNA:
gi_3421069 [Arabidopsis thaliana]
gi|12643647|sp|O81146|PS61_ARATH Proteasome subunit
alpha type 6-1 (20S proteasome alpha subunit A1)
gi|10176805|dbj|BAB09993.1| multicatalytic endopeptidase
complex alpha subunit-like [Arabidopsis thaliana]
Length = 246
Score = 372 bits (954), Expect(2) = e-117
Identities = 178/210 (84%), Positives = 198/210 (93%)
Frame = -1
Query: 921 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTQKKVPDKL 742
MSRGSG GYDRHITIFSPEGRLFQVEYAFKAVK AGITSIGVRGKDS+CVVTQKKVPDKL
Sbjct: 1 MSRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKTAGITSIGVRGKDSVCVVTQKKVPDKL 60
Query: 741 LDQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRHTYGYEMPVDVLAKWIAD 562
LDQ+SV+HLFPITKY+GL+ATG+TADAR+LVQQARN+AAEFR TYGYEMPVD+LAKWIAD
Sbjct: 61 LDQSSVTHLFPITKYIGLVATGITADARSLVQQARNQAAEFRFTYGYEMPVDILAKWIAD 120
Query: 561 KSQVYTQHAYMRPLGVVAMVLGIDDENGAQLYKCDPAGHYFGHKATSAGLKDQEAINFLE 382
KSQVYTQHAYMRPLGVVAMV+G+D+ENG LYKCDPAGH++GHKATSAG+K+QEA+NFLE
Sbjct: 121 KSQVYTQHAYMRPLGVVAMVMGVDEENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLE 180
Query: 381 KKMKNDPSFTYEETVQTAISALPSASSGGF 292
KKMK +PSFT++ETVQTAISAL S F
Sbjct: 181 KKMKENPSFTFDETVQTAISALQSVLQEDF 210
Score = 74.3 bits (181), Expect(2) = e-117
Identities = 35/42 (83%), Positives = 40/42 (94%)
Frame = -2
Query: 308 LLQEDFKATEIEVGVVRKDNPEFRVLSTDEIDEHLTAISERD 183
+LQEDFKATEIEVGVVR +NPEFR L+T+EI+EHLTAISERD
Sbjct: 205 VLQEDFKATEIEVGVVRAENPEFRALTTEEIEEHLTAISERD 246
>gb|AAM12968.1| multicatalytic endopeptidase complex alpha subunit-like
[Arabidopsis thaliana] gi|21386959|gb|AAM47883.1|
multicatalytic endopeptidase complex alpha subunit-like
[Arabidopsis thaliana]
Length = 246
Score = 370 bits (950), Expect(2) = e-117
Identities = 177/210 (84%), Positives = 198/210 (94%)
Frame = -1
Query: 921 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTQKKVPDKL 742
MSRGSG GYDRHITIFSPEGRLFQVEYAFKAVK AGITSIGVRGKDS+CVVTQK+VPDKL
Sbjct: 1 MSRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKTAGITSIGVRGKDSVCVVTQKEVPDKL 60
Query: 741 LDQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRHTYGYEMPVDVLAKWIAD 562
LDQ+SV+HLFPITKY+GL+ATG+TADAR+LVQQARN+AAEFR TYGYEMPVD+LAKWIAD
Sbjct: 61 LDQSSVTHLFPITKYIGLVATGITADARSLVQQARNQAAEFRFTYGYEMPVDILAKWIAD 120
Query: 561 KSQVYTQHAYMRPLGVVAMVLGIDDENGAQLYKCDPAGHYFGHKATSAGLKDQEAINFLE 382
KSQVYTQHAYMRPLGVVAMV+G+D+ENG LYKCDPAGH++GHKATSAG+K+QEA+NFLE
Sbjct: 121 KSQVYTQHAYMRPLGVVAMVMGVDEENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLE 180
Query: 381 KKMKNDPSFTYEETVQTAISALPSASSGGF 292
KKMK +PSFT++ETVQTAISAL S F
Sbjct: 181 KKMKENPSFTFDETVQTAISALQSVLQEDF 210
Score = 74.3 bits (181), Expect(2) = e-117
Identities = 35/42 (83%), Positives = 40/42 (94%)
Frame = -2
Query: 308 LLQEDFKATEIEVGVVRKDNPEFRVLSTDEIDEHLTAISERD 183
+LQEDFKATEIEVGVVR +NPEFR L+T+EI+EHLTAISERD
Sbjct: 205 VLQEDFKATEIEVGVVRAENPEFRALTTEEIEEHLTAISERD 246
>emb|CAA74025.1| multicatalytic endopeptidase complex, proteasome component, alpha
subunit [Arabidopsis thaliana]
Length = 245
Score = 370 bits (949), Expect(2) = e-117
Identities = 177/209 (84%), Positives = 197/209 (93%)
Frame = -1
Query: 918 SRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTQKKVPDKLL 739
SRGSG GYDRHITIFSPEGRLFQVEYAFKAVK AGITSIGVRGKDS+CVVTQKKVPDKLL
Sbjct: 1 SRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKTAGITSIGVRGKDSVCVVTQKKVPDKLL 60
Query: 738 DQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRHTYGYEMPVDVLAKWIADK 559
DQ+SV+HLFPITKY+GL+ATG+TADAR+LVQQARN+AAEFR TYGYEMPVD+LAKWIADK
Sbjct: 61 DQSSVTHLFPITKYIGLVATGITADARSLVQQARNQAAEFRFTYGYEMPVDILAKWIADK 120
Query: 558 SQVYTQHAYMRPLGVVAMVLGIDDENGAQLYKCDPAGHYFGHKATSAGLKDQEAINFLEK 379
SQVYTQHAYMRPLGVVAMV+G+D+ENG LYKCDPAGH++GHKATSAG+K+QEA+NFLEK
Sbjct: 121 SQVYTQHAYMRPLGVVAMVMGVDEENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEK 180
Query: 378 KMKNDPSFTYEETVQTAISALPSASSGGF 292
KMK +PSFT++ETVQTAISAL S F
Sbjct: 181 KMKENPSFTFDETVQTAISALQSVLQEDF 209
Score = 74.3 bits (181), Expect(2) = e-117
Identities = 35/42 (83%), Positives = 40/42 (94%)
Frame = -2
Query: 308 LLQEDFKATEIEVGVVRKDNPEFRVLSTDEIDEHLTAISERD 183
+LQEDFKATEIEVGVVR +NPEFR L+T+EI+EHLTAISERD
Sbjct: 204 VLQEDFKATEIEVGVVRAENPEFRALTTEEIEEHLTAISERD 245
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 942,901,269
Number of Sequences: 1393205
Number of extensions: 22279787
Number of successful extensions: 77365
Number of sequences better than 10.0: 409
Number of HSP's better than 10.0 without gapping: 63276
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74807
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 63464320210
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
