KMC003323A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC003323A_C01 KMC003323A_c01
agtaactatttaatcataaagtgaataaaacatgaccgataaatcaggttacatgtaaag
atcatttAAAACACTAGAAAAATCTAATCCTTTATTGTGTTTGCCAAACCTGGTCATAAT
CCCATGATTATCATCCAATTGAACCAGGCTGTGGTGATAGAAGTGGGTTCTTGCTTCCAT
GCAGAAACATTTCCCTGGATTTCAAGCAAGGACTGTTTTGGCCAAAAAAAAGGCAAGAAC
CATGTATTTTTACACAAATCTCAATAAGTTATACAAGGTAACTCAAAAATGTGTATACAA
TTTTCTGTGCCCTGAGATGCATGCACCTTGCAGTACAAAAATTAATCTCCCTTCAAAGAT
TAAATGGCAGAACTTCATGAAGTTTCTCCAGAGTTTTCATGCAGGATTTTCTCTGCCAGT
AACAAGTCATCAGGCGTAGTCACCTTGATATTGGTATAAGACCCTTCAGTGATATAAACA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003323A_C01 KMC003323A_c01
         (480 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_565286.1| putative sugar nucleotide phosphorylase; protei...    54  1e-06
pir||T00613 probable sugar nucleotide phosphorylase At2g02500 [i...    54  1e-06
ref|NP_387971.1| similar to hypothetical proteins [Bacillus subt...    44  0.001
sp|Q06755|ISPD_BACSU 2-C-methyl-D-erythritol 4-phosphate cytidyl...    44  0.001
ref|NP_349781.1| 4-diphosphocytidyl-2-methylerithritol synthase ...    43  0.002

>ref|NP_565286.1| putative sugar nucleotide phosphorylase; protein id: At2g02500.1,
           supported by cDNA: gi_12697582, supported by cDNA:
           gi_7385140 [Arabidopsis thaliana]
           gi|7385141|gb|AAF61714.1|AF230737_1
           4-Diphosphocytidyl-2C-methyl-D-erythritol synthase
           [Arabidopsis thaliana] gi|12697583|dbj|BAB21592.1|
           2-C-methyl-D-erythritol 4-phosphate cytidyltransferase
           [Arabidopsis thaliana] gi|20197092|gb|AAC18936.2|
           putative sugar nucleotide phosphorylase [Arabidopsis
           thaliana] gi|26451271|dbj|BAC42737.1| putative
           2-C-methyl-D-erythritol 4-phosphate cytidyltransferase
           [Arabidopsis thaliana]
          Length = 302

 Score = 53.5 bits (127), Expect = 1e-06
 Identities = 24/30 (80%), Positives = 29/30 (96%)
 Frame = -2

Query: 479 VYITEGSYTNIKVTTPDDLLLAEKILHENS 390
           VY+++GSYTNIKVTTPDDLLLAE+IL E+S
Sbjct: 273 VYVSQGSYTNIKVTTPDDLLLAERILSEDS 302

>pir||T00613 probable sugar nucleotide phosphorylase At2g02500 [imported] -
           Arabidopsis thaliana
          Length = 278

 Score = 53.5 bits (127), Expect = 1e-06
 Identities = 24/30 (80%), Positives = 29/30 (96%)
 Frame = -2

Query: 479 VYITEGSYTNIKVTTPDDLLLAEKILHENS 390
           VY+++GSYTNIKVTTPDDLLLAE+IL E+S
Sbjct: 249 VYVSQGSYTNIKVTTPDDLLLAERILSEDS 278

>ref|NP_387971.1| similar to hypothetical proteins [Bacillus subtilis]
           gi|2127058|pir||S66119 conserved hypothetical protein
           yacM - Bacillus subtilis gi|467478|dbj|BAA05324.1|
           unknown [Bacillus subtilis] gi|2632357|emb|CAB11866.1|
           yacM [Bacillus subtilis subsp. subtilis str. 168]
          Length = 232

 Score = 43.9 bits (102), Expect = 0.001
 Identities = 20/31 (64%), Positives = 23/31 (73%)
 Frame = -2

Query: 479 VYITEGSYTNIKVTTPDDLLLAEKILHENSG 387
           V + EGSYTNIK+TTPDDL  AE I+   SG
Sbjct: 198 VRVVEGSYTNIKLTTPDDLTSAEAIMESESG 228

>sp|Q06755|ISPD_BACSU 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
           (4-diphosphocytidyl-2C-methyl-D-erythritol synthase)
           (MEP cytidylyltransferase) (MCT)
           gi|289280|gb|AAA21794.1| unknown
          Length = 232

 Score = 43.9 bits (102), Expect = 0.001
 Identities = 20/31 (64%), Positives = 23/31 (73%)
 Frame = -2

Query: 479 VYITEGSYTNIKVTTPDDLLLAEKILHENSG 387
           V + EGSYTNIK+TTPDDL  AE I+   SG
Sbjct: 198 VRVVEGSYTNIKLTTPDDLTSAEAIMESESG 228

>ref|NP_349781.1| 4-diphosphocytidyl-2-methylerithritol synthase (Sugar Nucleotide
           Phosphorylase family) [Clostridium acetobutylicum]
           gi|20138681|sp|Q97EC9|ISPD_CLOAB 2-C-methyl-D-erythritol
           4-phosphate cytidylyltransferase
           (4-diphosphocytidyl-2C-methyl-D-erythritol synthase)
           (MEP cytidylyltransferase) (MCT) gi|25331383|pir||F97291
           4-diphosphocytidyl-2-methylerithritol synthase (Sugar
           Nucleotide Phosphorylase family) [imported] -
           Clostridium acetobutylicum
           gi|15026253|gb|AAK81121.1|AE007813_8
           4-diphosphocytidyl-2-methylerithritol synthase (Sugar
           Nucleotide Phosphorylase family) [Clostridium
           acetobutylicum]
          Length = 229

 Score = 43.1 bits (100), Expect = 0.002
 Identities = 18/30 (60%), Positives = 24/30 (80%)
 Frame = -2

Query: 479 VYITEGSYTNIKVTTPDDLLLAEKILHENS 390
           VY+ +GSY N+K+TTPDDL  AE +L +NS
Sbjct: 198 VYLYDGSYENLKITTPDDLYAAETLLKKNS 227

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 407,906,297
Number of Sequences: 1393205
Number of extensions: 8546199
Number of successful extensions: 19436
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 19098
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19436
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13397825772
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MR021d05_f BP077594 1 404
2 GNf034e11 BP069851 68 480




Lotus japonicus
Kazusa DNA Research Institute