>KMC003316A_C01 KMC003316A_c01
aagagacaaatatGGCAAGGAGGACATTTTTATTGCTGTAACATGTTCAAATGCCATATA
ATAGTACACATGAAAATGCAAATTAACTAGATTTTTTCACGCAAAACTTAAAAAAAAAAA
AGTTGAACTGGAACTTGATTAGTTCCAGAAATATATTACATTATCATTTCATCTTCAAAA
GTGAATGTTACAATATCTATAGTTATCATGCCTCATTCAACATAATCTCACAGATCACTG
CATCTGTTCTCAGATCTCAGAGAAGGAACTGATTGTCTTCAACCCGTGATTCTCAGGGAG
AGGTGCATTTCCAGGCCGAATAGAAATTATGACCTCCAGACCTGCTGCTTTAGCAGCTGT
AGCTTCTTGAAAAACATCAGTTACAAATAAGATATCTGATGGCTTATCAACGCCAAGTGA
TGCAGAAATTTCAACATAACTGTGTGTTTCTCTTTGGTTCCCCACTGTGGTATCAAAAAA
CCCAGATAAATATTTTCTTAGGTCCCCATGGTTTGTATGTCCAAATATTAGCCTTTGCGC
CAACCTGCTACCACTAGAATATATGTATACCTTTATGCCCAAGGCATGCCACTTTTCCAA
GGCTTCTGGTACATCATCAAAA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003316A_C01 KMC003316A_c01
         (622 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_200196.1| putative protein; protein id: At5g53850.1 [Arab...   199  2e-50
dbj|BAB62625.1| hypothetical protein~similar to Arabidopsis thal...   164  1e-39
gb|EAA09634.1| agCP15510 [Anopheles gambiae str. PEST]                114  1e-24
gb|EAA12926.1| agCP14292 [Anopheles gambiae str. PEST]                114  1e-24
ref|NP_649523.1| CG12173-PA [Drosophila melanogaster] gi|2317043...   114  1e-24

>ref|NP_200196.1| putative protein; protein id: At5g53850.1 [Arabidopsis thaliana]
           gi|10177247|dbj|BAB10715.1| contains similarity to
           enolase-phosphatase~gene_id:K19P17.1 [Arabidopsis
           thaliana]
          Length = 507

 Score =  199 bits (506), Expect = 2e-50
 Identities = 92/123 (74%), Positives = 113/123 (91%)
 Frame = -2

Query: 621 FDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGHTNHGDLRKYLSGFFDTTVGNQRETH 442
           F+DV +ALEKWH+ GIKVYIYSSGSRLAQ+L+FG+T++GDLRKY+SGFFDTT+GN++E+ 
Sbjct: 385 FEDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNTDYGDLRKYISGFFDTTIGNKKESR 444

Query: 441 SYVEISASLGVDKPSDILFVTDVFQEATAAKAAGLEVIISIRPGNAPLPENHGLKTISSF 262
           SY EI  +LGVD P++I+FVTDV+QEA AAKAAGLE IISIRPGNAPLPENHG KT++SF
Sbjct: 445 SYKEIKETLGVDDPAEIMFVTDVYQEAVAAKAAGLEAIISIRPGNAPLPENHGFKTVTSF 504

Query: 261 SEI 253
           S+I
Sbjct: 505 SQI 507

>dbj|BAB62625.1| hypothetical protein~similar to Arabidopsis thaliana chromosome 3,
            F28J7.34 [Oryza sativa (japonica cultivar-group)]
            gi|15408849|dbj|BAB64238.1| P0672D08.11 [Oryza sativa
            (japonica cultivar-group)] gi|20804430|dbj|BAB92127.1|
            hypothetical protein~similar to Arabidopsis thaliana
            chromosome 3, F28J7.34 [Oryza sativa (japonica
            cultivar-group)]
          Length = 1122

 Score =  164 bits (414), Expect = 1e-39
 Identities = 83/124 (66%), Positives = 98/124 (78%), Gaps = 1/124 (0%)
 Frame = -2

Query: 621  FDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGHTN-HGDLRKYLSGFFDTTVGNQRET 445
            +DD  +AL +W A   K YIYSSGSR AQRLIF +T  HGDLR +L GFFDTT+G +RE 
Sbjct: 1000 YDDAADALRRWRA---KAYIYSSGSREAQRLIFANTAAHGDLRDHLCGFFDTTIGAKREV 1056

Query: 444  HSYVEISASLGVDKPSDILFVTDVFQEATAAKAAGLEVIISIRPGNAPLPENHGLKTISS 265
             SY EI  +LG D+PS ILF+TDV+QEA AAK AGLEVIIS+RPGNAPLP+NHG  TI+S
Sbjct: 1057 SSYYEIWQTLGTDRPSQILFLTDVYQEAAAAKTAGLEVIISVRPGNAPLPDNHGFHTITS 1116

Query: 264  FSEI 253
            F+EI
Sbjct: 1117 FAEI 1120

>gb|EAA09634.1| agCP15510 [Anopheles gambiae str. PEST]
          Length = 691

 Score =  114 bits (285), Expect = 1e-24
 Identities = 60/125 (48%), Positives = 82/125 (65%), Gaps = 2/125 (1%)
 Frame = -2

Query: 621 FDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGHTNHGDLRKYLSGFFDTTVGNQRETH 442
           +DDV +A E+W   G K+YIYSSGS  AQ+L+F H+  GDL KYLSG +DT +G +RE  
Sbjct: 138 YDDVQKAFEQWTENGRKIYIYSSGSVDAQKLLFEHSEQGDLLKYLSGHYDTKIGAKREKE 197

Query: 441 SYVEISASLGVDKPSDILFVTDVFQEATAAKAAGLEVIISIRPGNAPLPENH--GLKTIS 268
           SY  I  ++    P + LF+TDV+ EA AAK AGL V++  RPGN+ L E        I+
Sbjct: 198 SYTSILKNI-ESSPEEALFLTDVYAEAKAAKEAGLNVVLLDRPGNSELSEEERKDFPVIA 256

Query: 267 SFSEI 253
           +FS++
Sbjct: 257 TFSDL 261

>gb|EAA12926.1| agCP14292 [Anopheles gambiae str. PEST]
          Length = 691

 Score =  114 bits (285), Expect = 1e-24
 Identities = 60/125 (48%), Positives = 82/125 (65%), Gaps = 2/125 (1%)
 Frame = -2

Query: 621 FDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGHTNHGDLRKYLSGFFDTTVGNQRETH 442
           +DDV +A E+W   G K+YIYSSGS  AQ+L+F H+  GDL KYLSG +DT +G +RE  
Sbjct: 138 YDDVQKAFEQWTENGRKIYIYSSGSVDAQKLLFEHSEQGDLLKYLSGHYDTKIGAKREKE 197

Query: 441 SYVEISASLGVDKPSDILFVTDVFQEATAAKAAGLEVIISIRPGNAPLPENH--GLKTIS 268
           SY  I  ++    P + LF+TDV+ EA AAK AGL V++  RPGN+ L E        I+
Sbjct: 198 SYTSILKNI-ESSPEEALFLTDVYAEAKAAKEAGLNVVLLDRPGNSELSEEERKDFPVIA 256

Query: 267 SFSEI 253
           +FS++
Sbjct: 257 TFSDL 261

>ref|NP_649523.1| CG12173-PA [Drosophila melanogaster] gi|23170430|gb|AAF52053.2|
           CG12173-PA [Drosophila melanogaster]
          Length = 256

 Score =  114 bits (284), Expect = 1e-24
 Identities = 60/125 (48%), Positives = 83/125 (66%), Gaps = 2/125 (1%)
 Frame = -2

Query: 621 FDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGHTNHGDLRKYLSGFFDTTVGNQRETH 442
           ++DVP A E W A G+++ +YSSGS  AQ+LIFGH+  G+L+ YLS +FDT VG+++E  
Sbjct: 121 YEDVPAAFEAWRAAGLQIAVYSSGSVAAQKLIFGHSLAGNLQPYLSAYFDTHVGHKQEQQ 180

Query: 441 SYVEISASLGVDKPSDILFVTDVFQEATAAKAAGLEVIISIRPGNAPLPENH--GLKTIS 268
           SY  I+  L  D P  ILF+TD+  EA AA+ AGL+ II  RPGNA L ++   G + I 
Sbjct: 181 SYKNIAKQLKED-PKQILFLTDIPGEAAAARCAGLQAIILKRPGNAALADDQKTGFELIP 239

Query: 267 SFSEI 253
            F  +
Sbjct: 240 DFKPL 244

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 530,552,238
Number of Sequences: 1393205
Number of extensions: 11363733
Number of successful extensions: 23639
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 22969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23600
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25017613016
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR033d05_f BP078541 1 389 2 GNf034c11 BP069832 14 396 3 SPD057c03_f BP048520 63 625

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