>KMC003299A_C02 KMC003299A_c02
agaagatagtAGATTAAAACACAGGGCAAACATTATAACAATCAAATGAAAAACACACTC
TTTATTGTGTCAAGACAGTGCCATCAGAATTGTACAAAAAATGAACAACATTCTCAGCCA
CTCTTTTTTGGATGGCTTGATTTTACAAACATACGCTCTAAATTTCCAAATTTCTGGGAT
GGGAAGGGAAATATGACTTGCCATGAAAATACCATAAAACAAGGCATTGTCTTATAATGG
AAAGCTAAGCGGCAGCAGTAATGCACTGTATTGCCTCTTTGCATATACCAGTGCATTGTT
GCTTCAGGTCCATAAACCTCAATGGGGATGCCAATTTCCTCTCCAAGTGCTCGCATTAAA
GCTAGACCCTTCTGGTAGTTCGGACCCCCTCTCCTCACGTAAATGTGCATCCTTGCCGCT
TTTAACTTTGATTCCTTCTCCTTTAGTGCCCGAATTATGCCGCTAAATGTAGCAGCAACA
TCAGTAAAGTTAGCTATGCCTCCTCCTATCACAAGAGCTCTCTTTTGGCCATCAGGGTTT
GCAGTTGCACAATCAATCACAACTCTGGCATACTGCAAGACCTCATCTTCCTTGGGTGCA
CCACTATACTCGGCATAATTTCCAAGTTCAGATGC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003299A_C02 KMC003299A_c02
         (635 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAC86996.1| ATP citrate lyase b-subunit [Lupinus albus]           221  6e-57
ref|NP_172537.1| ATP citrate-lyase, putative; protein id: At1g10...   214  6e-55
ref|NP_176280.1| ATP citrate-lyase, putative; protein id: At1g60...   214  6e-55
gb|AAL91162.1| similar to ATP-citrate-lyase [Arabidopsis thalian...   214  6e-55
gb|AAM65078.1| ATP citrate-lyase, putative [Arabidopsis thaliana]     211  5e-54

>emb|CAC86996.1| ATP citrate lyase b-subunit [Lupinus albus]
          Length = 423

 Score =  221 bits (563), Expect = 6e-57
 Identities = 110/114 (96%), Positives = 112/114 (97%)
 Frame = -1

Query: 635 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGI 456
           A+ELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALV+GGGIANFTDVAATFSGI
Sbjct: 295 ANELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVVGGGIANFTDVAATFSGI 354

Query: 455 IRALKEKESKLKAARMHIYVRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATM 294
           IRALKEKESKLKAARMHIYVRRGGPNYQKGLA MR LGEEIGIPIEVYGPEATM
Sbjct: 355 IRALKEKESKLKAARMHIYVRRGGPNYQKGLAKMRELGEEIGIPIEVYGPEATM 408

 Score = 34.7 bits (78), Expect = 1.1
 Identities = 16/33 (48%), Positives = 21/33 (63%)
 Frame = -2

Query: 346 LERKLASPLRFMDLKQQCTGICKEAIQCITAAA 248
           L  ++  P+     +   TGICKEAIQCITA+A
Sbjct: 391 LGEEIGIPIEVYGPEATMTGICKEAIQCITASA 423

>ref|NP_172537.1| ATP citrate-lyase, putative; protein id: At1g10670.1, supported by
           cDNA: 36439., supported by cDNA: gi_15810348, supported
           by cDNA: gi_16648641, supported by cDNA: gi_20453213,
           supported by cDNA: gi_21280802 [Arabidopsis thaliana]
           gi|6573737|gb|AAF17657.1|AC009398_6 F20B24.11
           [Arabidopsis thaliana] gi|15810349|gb|AAL07062.1|
           putative ATP citrate-lyase [Arabidopsis thaliana]
           gi|16648642|gb|AAL25637.1| ATP-citrate lyase subunit A
           [Arabidopsis thaliana] gi|20453214|gb|AAM19846.1|
           At1g10670/F20B24_11 [Arabidopsis thaliana]
           gi|21280803|gb|AAM45027.1| putative ATP citrate-lyase
           [Arabidopsis thaliana]
          Length = 423

 Score =  214 bits (546), Expect = 6e-55
 Identities = 105/114 (92%), Positives = 112/114 (98%)
 Frame = -1

Query: 635 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGI 456
           ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDG+ RALVIGGGIANFTDVAATF+GI
Sbjct: 295 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGKSRALVIGGGIANFTDVAATFNGI 354

Query: 455 IRALKEKESKLKAARMHIYVRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATM 294
           IRALKEKE+KLKAARMHI+VRRGGPNYQKGLA MRALG++IG+PIEVYGPEATM
Sbjct: 355 IRALKEKEAKLKAARMHIFVRRGGPNYQKGLAKMRALGDDIGVPIEVYGPEATM 408

>ref|NP_176280.1| ATP citrate-lyase, putative; protein id: At1g60810.1, supported by
           cDNA: gi_19698852 [Arabidopsis thaliana]
           gi|25404292|pir||F96633 hypothetical protein F8A5.32
           [imported] - Arabidopsis thaliana
           gi|2462746|gb|AAB71965.1| Similar to ATP-citrate-lyase
           [Arabidopsis thaliana]
          Length = 423

 Score =  214 bits (546), Expect = 6e-55
 Identities = 105/114 (92%), Positives = 112/114 (98%)
 Frame = -1

Query: 635 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGI 456
           ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDG+ RALVIGGGIANFTDVAATF+GI
Sbjct: 295 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGKSRALVIGGGIANFTDVAATFNGI 354

Query: 455 IRALKEKESKLKAARMHIYVRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATM 294
           IRALKEKE+KLKAARMHI+VRRGGPNYQKGLA MR+LG+EIG+PIEVYGPEATM
Sbjct: 355 IRALKEKEAKLKAARMHIFVRRGGPNYQKGLAKMRSLGDEIGVPIEVYGPEATM 408

>gb|AAL91162.1| similar to ATP-citrate-lyase [Arabidopsis thaliana]
           gi|22136126|gb|AAM91141.1| similar to ATP-citrate-lyase
           [Arabidopsis thaliana]
          Length = 423

 Score =  214 bits (546), Expect = 6e-55
 Identities = 105/114 (92%), Positives = 112/114 (98%)
 Frame = -1

Query: 635 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGI 456
           ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDG+ RALVIGGGIANFTDVAATF+GI
Sbjct: 295 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGKSRALVIGGGIANFTDVAATFNGI 354

Query: 455 IRALKEKESKLKAARMHIYVRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATM 294
           IRALKEKE+KLKAARMHI+VRRGGPNYQKGLA MR+LG+EIG+PIEVYGPEATM
Sbjct: 355 IRALKEKEAKLKAARMHIFVRRGGPNYQKGLAKMRSLGDEIGVPIEVYGPEATM 408

>gb|AAM65078.1| ATP citrate-lyase, putative [Arabidopsis thaliana]
          Length = 423

 Score =  211 bits (538), Expect = 5e-54
 Identities = 104/114 (91%), Positives = 111/114 (97%)
 Frame = -1

Query: 635 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGQKRALVIGGGIANFTDVAATFSGI 456
           ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDG+ RALVIGGGIANFTDVAATF+GI
Sbjct: 295 ASELGNYAEYSGAPKEDEVLQYARVVIDCATANPDGKSRALVIGGGIANFTDVAATFNGI 354

Query: 455 IRALKEKESKLKAARMHIYVRRGGPNYQKGLALMRALGEEIGIPIEVYGPEATM 294
           IRALKEKE+KLKAARMHI+VRRG PNYQKGLA MRALG++IG+PIEVYGPEATM
Sbjct: 355 IRALKEKEAKLKAARMHIFVRRGRPNYQKGLAKMRALGDDIGVPIEVYGPEATM 408

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 561,758,177
Number of Sequences: 1393205
Number of extensions: 12627243
Number of successful extensions: 38165
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 37273
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38142
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26439068301
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD025c07_f AV771694 1 495 2 MR055d03_f BP080234 11 355 3 SPD024g07_f BP045920 28 579 4 SPDL092g09_f BP057794 37 532 5 MF100h12_f BP033518 41 572 6 SPD079f08_f BP050337 48 644 7 GNf032g08 BP069720 48 129 8 SPD029g06_f BP046326 48 403 9 MF012a01_f BP028836 48 371 10 MFB021d01_f BP035491 48 605 11 MR059a06_f BP080496 52 339 12 MF058f04_f BP031367 57 600 13 MWM112f10_f AV766519 58 503

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