Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003251A_C01 KMC003251A_c01
(677 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL07434.1|AF413213_1 prunasin hydrolase isoform PH C precurs... 215 5e-55
gb|AAL35324.1|AF411131_1 prunasin hydrolase isoform PH C precurs... 215 5e-55
pir||T09657 beta-glucosidase (EC 3.2.1.21) precursor, cyanogenic... 214 9e-55
emb|CAC08209.2| beta-glucosidase [Cicer arietinum] 214 9e-55
gb|AAL07491.1|AF414608_1 prunasin hydrolase isoform PH I precurs... 214 9e-55
>gb|AAL07434.1|AF413213_1 prunasin hydrolase isoform PH C precursor [Prunus serotina]
Length = 517
Score = 215 bits (547), Expect = 5e-55
Identities = 95/131 (72%), Positives = 116/131 (88%)
Frame = -3
Query: 657 PIGQRAASSWLYVYPKGIQELLLYIKKKYNNPLIYITENGMSDFNDPTLSLEEALLDTFR 478
PIG RAAS WLYVYPKG+ +L+LY K+KYN+P++YITENGM +FN+P LSLE+AL D R
Sbjct: 362 PIGPRAASDWLYVYPKGLYDLVLYTKEKYNDPIMYITENGMDEFNNPKLSLEQALDDVNR 421
Query: 477 IDYYYRHLFYLQSAIRDGANVKGYFAWSLLDNFEWASGYTLRFGINFVDYKNGLKRYQKL 298
IDYYYRHL YLQ+AI++GANV+GYFAWSLLDNFEW+ GYT+RFGIN++DY NGL+R+ KL
Sbjct: 422 IDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLERHSKL 481
Query: 297 SAKWFKNFLKR 265
S WFK+FLKR
Sbjct: 482 STHWFKSFLKR 492
>gb|AAL35324.1|AF411131_1 prunasin hydrolase isoform PH C precursor [Prunus serotina]
Length = 542
Score = 215 bits (547), Expect = 5e-55
Identities = 95/131 (72%), Positives = 116/131 (88%)
Frame = -3
Query: 657 PIGQRAASSWLYVYPKGIQELLLYIKKKYNNPLIYITENGMSDFNDPTLSLEEALLDTFR 478
PIG RAAS WLYVYPKG+ +L+LY K+KYN+P++YITENGM +FN+P LSLE+AL D R
Sbjct: 387 PIGPRAASDWLYVYPKGLYDLVLYTKEKYNDPIMYITENGMDEFNNPKLSLEQALDDVNR 446
Query: 477 IDYYYRHLFYLQSAIRDGANVKGYFAWSLLDNFEWASGYTLRFGINFVDYKNGLKRYQKL 298
IDYYYRHL YLQ+AI++GANV+GYFAWSLLDNFEW+ GYT+RFGIN++DY NGL+R+ KL
Sbjct: 447 IDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLERHSKL 506
Query: 297 SAKWFKNFLKR 265
S WFK+FLKR
Sbjct: 507 STHWFKSFLKR 517
>pir||T09657 beta-glucosidase (EC 3.2.1.21) precursor, cyanogenic - black cherry
gi|1236961|gb|AAA93032.1| prunasin hydrolase isoform PH
I precursor [Prunus serotina]
Length = 549
Score = 214 bits (545), Expect = 9e-55
Identities = 95/131 (72%), Positives = 116/131 (88%)
Frame = -3
Query: 657 PIGQRAASSWLYVYPKGIQELLLYIKKKYNNPLIYITENGMSDFNDPTLSLEEALLDTFR 478
PIG +AAS WLYVYPKG+ +L+LY K+KYN+P++YITENGM +FN+P LSLEEAL D R
Sbjct: 394 PIGPQAASDWLYVYPKGLYDLVLYTKEKYNDPVMYITENGMDEFNNPKLSLEEALDDANR 453
Query: 477 IDYYYRHLFYLQSAIRDGANVKGYFAWSLLDNFEWASGYTLRFGINFVDYKNGLKRYQKL 298
IDYYYRHL YLQ+AI++GANV+GYFAWSLLDNFEW+ GYT+RFGIN++DY NGL+R+ KL
Sbjct: 454 IDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLERHSKL 513
Query: 297 SAKWFKNFLKR 265
S WFK+FLKR
Sbjct: 514 STHWFKSFLKR 524
>emb|CAC08209.2| beta-glucosidase [Cicer arietinum]
Length = 439
Score = 214 bits (545), Expect = 9e-55
Identities = 93/131 (70%), Positives = 117/131 (88%)
Frame = -3
Query: 657 PIGQRAASSWLYVYPKGIQELLLYIKKKYNNPLIYITENGMSDFNDPTLSLEEALLDTFR 478
P+G RAASSWLYVYP+G+++LLL+IK+KYNNP IYI ENGM +FNDPTL ++EALLDTFR
Sbjct: 308 PLGPRAASSWLYVYPRGLRDLLLHIKEKYNNPAIYIHENGMDEFNDPTLPVKEALLDTFR 367
Query: 477 IDYYYRHLFYLQSAIRDGANVKGYFAWSLLDNFEWASGYTLRFGINFVDYKNGLKRYQKL 298
IDYY+RHL+Y++SAI+ GANVK + AWSL DNFEW GY RFG+N++DYK+GLKRY K+
Sbjct: 368 IDYYFRHLYYIRSAIQLGANVKAFLAWSLFDNFEWGGGYQHRFGLNYIDYKDGLKRYPKV 427
Query: 297 SAKWFKNFLKR 265
SA+W++NFLKR
Sbjct: 428 SAQWYQNFLKR 438
>gb|AAL07491.1|AF414608_1 prunasin hydrolase isoform PH I precursor [Prunus serotina]
Length = 513
Score = 214 bits (545), Expect = 9e-55
Identities = 95/131 (72%), Positives = 116/131 (88%)
Frame = -3
Query: 657 PIGQRAASSWLYVYPKGIQELLLYIKKKYNNPLIYITENGMSDFNDPTLSLEEALLDTFR 478
PIG +AAS WLYVYPKG+ +L+LY K+KYN+P++YITENGM +FN+P LSLEEAL D R
Sbjct: 358 PIGPQAASDWLYVYPKGLYDLVLYTKEKYNDPVMYITENGMDEFNNPKLSLEEALDDANR 417
Query: 477 IDYYYRHLFYLQSAIRDGANVKGYFAWSLLDNFEWASGYTLRFGINFVDYKNGLKRYQKL 298
IDYYYRHL YLQ+AI++GANV+GYFAWSLLDNFEW+ GYT+RFGIN++DY NGL+R+ KL
Sbjct: 418 IDYYYRHLCYLQAAIKEGANVQGYFAWSLLDNFEWSEGYTVRFGINYIDYDNGLERHSKL 477
Query: 297 SAKWFKNFLKR 265
S WFK+FLKR
Sbjct: 478 STHWFKSFLKR 488
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 584,566,795
Number of Sequences: 1393205
Number of extensions: 12270656
Number of successful extensions: 30624
Number of sequences better than 10.0: 520
Number of HSP's better than 10.0 without gapping: 29000
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30203
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 29987172312
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
