Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003250A_C01 KMC003250A_c01
(552 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAA63963.1| glutamate synthetase; glutamate--ammonia ligase ... 352 2e-96
sp|Q42899|GLN1_LOTJA Glutamine synthetase, cytosolic isozyme (Gl... 352 2e-96
emb|CAA73366.1| glutamine synthetase [Lotus japonicus] 351 4e-96
gb|AAG24873.1|AF301590_1 cytosolic glutamine synthetase GSbeta1 ... 339 2e-92
pir||S49976 glutamate-ammonia ligase (EC 6.3.1.2) - rape gi|5996... 338 2e-92
>emb|CAA63963.1| glutamate synthetase; glutamate--ammonia ligase [Lotus japonicus]
Length = 356
Score = 352 bits (903), Expect = 2e-96
Identities = 166/166 (100%), Positives = 166/166 (100%)
Frame = +2
Query: 53 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 232
MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST
Sbjct: 1 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 60
Query: 233 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 412
GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV
Sbjct: 61 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 120
Query: 413 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 550
AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA
Sbjct: 121 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 166
>sp|Q42899|GLN1_LOTJA Glutamine synthetase, cytosolic isozyme (Glutamate--ammonia ligase)
(GS1)
Length = 356
Score = 352 bits (903), Expect = 2e-96
Identities = 166/166 (100%), Positives = 166/166 (100%)
Frame = +2
Query: 53 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 232
MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST
Sbjct: 1 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 60
Query: 233 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 412
GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV
Sbjct: 61 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 120
Query: 413 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 550
AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA
Sbjct: 121 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 166
>emb|CAA73366.1| glutamine synthetase [Lotus japonicus]
Length = 356
Score = 351 bits (900), Expect = 4e-96
Identities = 165/166 (99%), Positives = 166/166 (99%)
Frame = +2
Query: 53 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 232
MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMR+KARTLPGPVSDPSQLPKWNYDGSST
Sbjct: 1 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRTKARTLPGPVSDPSQLPKWNYDGSST 60
Query: 233 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 412
GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV
Sbjct: 61 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 120
Query: 413 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 550
AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA
Sbjct: 121 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 166
>gb|AAG24873.1|AF301590_1 cytosolic glutamine synthetase GSbeta1 [Glycine max]
Length = 356
Score = 339 bits (869), Expect = 2e-92
Identities = 153/166 (92%), Positives = 165/166 (99%)
Frame = +2
Query: 53 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 232
MSLLSDLINLNLS+TT+K+IAEYIWIGGSG+D+RSKARTLPGPVSDPS+LPKWNYDGSST
Sbjct: 1 MSLLSDLINLNLSDTTEKVIAEYIWIGGSGMDLRSKARTLPGPVSDPSELPKWNYDGSST 60
Query: 233 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 412
GQAPGEDSEVILYPQAIFRDPFRRG+NILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV
Sbjct: 61 GQAPGEDSEVILYPQAIFRDPFRRGNNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 120
Query: 413 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 550
AEVPWYGIEQEYTLLQK++ WP+GWP+GGFPGPQGPYYCG+GADKA
Sbjct: 121 AEVPWYGIEQEYTLLQKDIQWPLGWPVGGFPGPQGPYYCGVGADKA 166
>pir||S49976 glutamate-ammonia ligase (EC 6.3.1.2) - rape
gi|599656|emb|CAA58118.1| glutamate--ammonia ligase
[Brassica napus]
Length = 356
Score = 338 bits (868), Expect = 2e-92
Identities = 153/166 (92%), Positives = 165/166 (99%)
Frame = +2
Query: 53 MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 232
MSLL+DL+NLNLSETTDKIIAEYIW+GGSG+DMRSKARTLPGPVSDPS+LPKWNYDGSST
Sbjct: 1 MSLLTDLVNLNLSETTDKIIAEYIWVGGSGMDMRSKARTLPGPVSDPSELPKWNYDGSST 60
Query: 233 GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 412
GQAPGEDSEVILYPQAIF+DPFRRG+NILV+CDAYTPAGEPIPTNKRHAAAKVFSHPDVV
Sbjct: 61 GQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKVFSHPDVV 120
Query: 413 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKA 550
AEVPWYGIEQEYTLLQK+VNWP+GWPIGGFPGPQGPYYC +GADK+
Sbjct: 121 AEVPWYGIEQEYTLLQKDVNWPLGWPIGGFPGPQGPYYCSVGADKS 166
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 559,023,460
Number of Sequences: 1393205
Number of extensions: 14085070
Number of successful extensions: 62644
Number of sequences better than 10.0: 403
Number of HSP's better than 10.0 without gapping: 47752
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 59657
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19234190289
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
