Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003244A_C01 KMC003244A_c01
(555 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM91597.1| putative protein [Arabidopsis thaliana] 174 5e-43
ref|NP_193885.1| putative protein; protein id: At4g21540.1 [Arab... 151 6e-36
dbj|BAC20872.1| putative sphingosine kinase [Oryza sativa (japon... 124 6e-28
gb|AAO50996.1| similar to Homo sapiens (Human). Hypothetical pro... 65 4e-10
gb|AAK39587.1|AC025296_22 putative sphingosine kinase [Oryza sat... 53 3e-06
>gb|AAM91597.1| putative protein [Arabidopsis thaliana]
Length = 485
Score = 174 bits (442), Expect = 5e-43
Identities = 82/114 (71%), Positives = 103/114 (89%)
Frame = -3
Query: 553 AEDTMAAPDAKFSDGYLDLIITKNCPKLQLLSMMSELNKGGHVKSPYVTYLKVKAFSLEP 374
AE+T+AAPDAKFSDG+LDLI+ K+CPKL LLS+M++L+ G HV+SPY +YLKVKAF LEP
Sbjct: 371 AENTLAAPDAKFSDGFLDLIVMKDCPKLALLSLMTKLSDGTHVQSPYASYLKVKAFVLEP 430
Query: 373 GPRTKDKEKEGIIDSDGEVLARGKGTYKCEQKSLMAYDKLQITVDKGLATLFTP 212
G R + +KEGIIDSDGEVLARG+ +YKC+QK+LM+YDKLQI+VD+GLATLF+P
Sbjct: 431 GARIDEPDKEGIIDSDGEVLARGRKSYKCDQKALMSYDKLQISVDQGLATLFSP 484
>ref|NP_193885.1| putative protein; protein id: At4g21540.1 [Arabidopsis thaliana]
gi|7485713|pir||T05162 hypothetical protein F18E5.160 -
Arabidopsis thaliana gi|3080398|emb|CAA18718.1| putative
protein [Arabidopsis thaliana]
gi|7268951|emb|CAB81261.1| putative protein [Arabidopsis
thaliana]
Length = 1240
Score = 151 bits (381), Expect = 6e-36
Identities = 73/111 (65%), Positives = 90/111 (80%), Gaps = 5/111 (4%)
Frame = -3
Query: 553 AEDTMAAPDAKFSDGYLDLIITKNCPKLQLLSMMSELNKGGHVKSPYVTYLK-----VKA 389
+E+T+ AP AKFSDGYLDLI+ KNCPKL LLS+M + + G HV+SPY+ Y+K VKA
Sbjct: 648 SENTLTAPAAKFSDGYLDLIVLKNCPKLVLLSLMRQTSSGTHVESPYIVYIKLTVEKVKA 707
Query: 388 FSLEPGPRTKDKEKEGIIDSDGEVLARGKGTYKCEQKSLMAYDKLQITVDK 236
F LEPG + +KEGIIDSDGEVLARGK TYKC+QK+LM+YDKLQ+TVD+
Sbjct: 708 FVLEPGALVDEPDKEGIIDSDGEVLARGKRTYKCDQKALMSYDKLQVTVDQ 758
Score = 100 bits (248), Expect = 2e-20
Identities = 47/63 (74%), Positives = 57/63 (89%)
Frame = -3
Query: 400 KVKAFSLEPGPRTKDKEKEGIIDSDGEVLARGKGTYKCEQKSLMAYDKLQITVDKGLATL 221
KVKAF LEPG R + +KEGIIDSDGEVLARG+ +YKC+QK+LM+YDKLQI+VD+GLATL
Sbjct: 1177 KVKAFVLEPGARIDEPDKEGIIDSDGEVLARGRKSYKCDQKALMSYDKLQISVDQGLATL 1236
Query: 220 FTP 212
F+P
Sbjct: 1237 FSP 1239
>dbj|BAC20872.1| putative sphingosine kinase [Oryza sativa (japonica
cultivar-group)]
Length = 480
Score = 124 bits (312), Expect = 6e-28
Identities = 62/116 (53%), Positives = 84/116 (71%), Gaps = 2/116 (1%)
Frame = -3
Query: 553 AEDTMAAPDAKFSDGYLDLIITKNCPKLQLLSMMSELNKGGHVKSPYVTYLKVKAFSLEP 374
+E+ M AP+AKF DGYLD+ I K+CP+ L +M ++ G +VKSPYV Y KVKA +EP
Sbjct: 364 SENVMTAPEAKFGDGYLDVAIIKDCPRSALAGLMFQMKDGSYVKSPYVEYFKVKALRIEP 423
Query: 373 GPRTKDKEKEGIIDSDGEVLARGKGTYKCEQ-KSLMAY-DKLQITVDKGLATLFTP 212
G R K GIIDSDGEV+ARG G++ ++ + LMAY +Q+TVD+GLAT+F+P
Sbjct: 424 GMRVGSTTKGGIIDSDGEVIARGDGSHTGDEIEHLMAYGPPIQLTVDQGLATIFSP 479
>gb|AAO50996.1| similar to Homo sapiens (Human). Hypothetical protein
[Dictyostelium discoideum]
Length = 624
Score = 65.5 bits (158), Expect = 4e-10
Identities = 36/92 (39%), Positives = 55/92 (59%), Gaps = 3/92 (3%)
Frame = -3
Query: 553 AEDTMAAPDAKFSDGYLDLIITKNCPKLQ---LLSMMSELNKGGHVKSPYVTYLKVKAFS 383
A D +A+P A SDG +DL++ N K LLS+++E + G HVKS + KV+A
Sbjct: 526 ASDFIASPTAHLSDGLIDLVVINNNKKFSKAGLLSVLTESSTGAHVKSDLIDQYKVQAMI 585
Query: 382 LEPGPRTKDKEKEGIIDSDGEVLARGKGTYKC 287
LEP ++EGII DGE+++ G+ + +C
Sbjct: 586 LEP-----SNDREGIIAVDGELISYGRTSMEC 612
>gb|AAK39587.1|AC025296_22 putative sphingosine kinase [Oryza sativa]
Length = 757
Score = 52.8 bits (125), Expect = 3e-06
Identities = 32/83 (38%), Positives = 44/83 (52%)
Frame = -3
Query: 535 APDAKFSDGYLDLIITKNCPKLQLLSMMSELNKGGHVKSPYVTYLKVKAFSLEPGPRTKD 356
AP A++ D LDL++ +L+LL + L G H+ P V YLKVK+ L+ GP T D
Sbjct: 662 APKAEYDDNCLDLLLVGGSGRLRLLKFLVLLQFGKHISLPNVEYLKVKSVRLKAGPNTHD 721
Query: 355 KEKEGIIDSDGEVLARGKGTYKC 287
DGE+L KG +C
Sbjct: 722 G-----CGIDGELL-HVKGQVRC 738
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 495,772,300
Number of Sequences: 1393205
Number of extensions: 11025475
Number of successful extensions: 22642
Number of sequences better than 10.0: 82
Number of HSP's better than 10.0 without gapping: 21973
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22618
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19521267756
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
