>KMC003242A_C01 KMC003242A_c01
ataaagaaacaagatcccatcctcttatctatctatgcatgtcaagggtgtgtgttatgc
cagtgacagtagcaggttaAGAAGCAAATTGGAAAATACATCTCATTTAAAAGAATATAG
AACTTTACACTTCTACAATGAAGCAATGCGGAAAGTCAGAATGCAGAGGTTTCTAACAGT
GTCAATCATTCTGAAGATGGTGAAGAGAATAGAAGTTTCAAAAGAAAGCCAAGCAAAAGT
CCAAGGAGACCCACAAAAACAGCAAAAGTGAAGGAAAAGCCAGGATCGCCGCCACCTCTT
CGGTTTCTTTGCCTTTTCAGCATGTCCAGTTCCTGTTGCAGCATTCGCGTTTGTCTTGCA
GCGACATCTCTTTCTTCTTTTAGTTGCTCTAAAGCCTGGCTAGAAGAGGTACCAAGTTTT
TGTGCGGAGTTCTTCAAACCATCCTCTTCAGAGCTTCCATCAGGTGAGGTAGGCGTGATA
TATGCAACTCGTAGTTTCAACTCCTCTATTGAATTACCACTATCCTTACTAAAAGTATCT
GGTGGAAGGTCATCAACATCAGTATTTGGATTGACTATAGTACTCTGTAAGAGAAACTTG
TCTTTACATTGCATATCTGGAGGGAATTCACGTTGGGCTTGGAGGGTCACTCTGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003242A_C01 KMC003242A_c01
         (655 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_199529.1| VAMP (vesicle-associated membrane protein)-asso...   173  2e-42
gb|AAO32056.1| putative VAMP-associated protein [Brassica rapa s...   171  1e-41
gb|AAK43500.1|AC020666_10 putative membrane protein [Oryza sativ...   149  2e-35
emb|CAC39038.1| putative vesicle-associated membrane protein (VA...   149  3e-35
pir||JC7234 27k vesicle-associated membrane protein-associated p...   117  1e-25

>ref|NP_199529.1| VAMP (vesicle-associated membrane protein)-associated protein-like;
           protein id: At5g47180.2, supported by cDNA: 14236.,
           supported by cDNA: gi_17104632 [Arabidopsis thaliana]
           gi|8809600|dbj|BAA97151.1| VAMP (vesicle-associated
           membrane protein)-associated protein-like [Arabidopsis
           thaliana] gi|14334974|gb|AAK59664.1| putative VAMP
           (vesicle-associated membrane protein)-associated protein
           [Arabidopsis thaliana] gi|17104633|gb|AAL34205.1|
           putative VAMP-associated protein [Arabidopsis thaliana]
           gi|21553413|gb|AAM62506.1| VAMP (vesicle-associated
           membrane protein)-associated protein-like [Arabidopsis
           thaliana]
          Length = 220

 Score =  173 bits (439), Expect = 2e-42
 Identities = 87/153 (56%), Positives = 115/153 (74%)
 Frame = -3

Query: 653 RVTLQAQREFPPDMQCKDKFLLQSTIVNPNTDVDDLPPDTFSKDSGNSIEELKLRVAYIT 474
           RVTLQAQRE+PPDMQCKDKFLLQSTIV P+TDVD+LP DTF+KDSG ++ E KL+V+YIT
Sbjct: 71  RVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVSYIT 130

Query: 473 PTSPDGSSEEDGLKNSAQKLGTSSSQALEQLKEERDVAARQTRMLQQELDMLKRQRNRRG 294
           P++   SSE        Q   T S+  +++LKEERD A +QT+ LQ EL+ ++R+RN+R 
Sbjct: 131 PSTTQRSSESGATNGDGQSSETIST--IQRLKEERDAAVKQTQQLQHELETVRRRRNQRN 188

Query: 293 GGDPGFSFTFAVFVGLLGLLLGFLLKLLFSSPS 195
            G+ G S   A  VGL+GL++GF+LKL  +SP+
Sbjct: 189 SGN-GLSLKLAAMVGLIGLIIGFILKLTLASPT 220

>gb|AAO32056.1| putative VAMP-associated protein [Brassica rapa subsp. pekinensis]
          Length = 165

 Score =  171 bits (432), Expect = 1e-41
 Identities = 85/153 (55%), Positives = 115/153 (74%)
 Frame = -3

Query: 653 RVTLQAQREFPPDMQCKDKFLLQSTIVNPNTDVDDLPPDTFSKDSGNSIEELKLRVAYIT 474
           RVTLQAQRE+PPDMQCKDKFLLQSTIV P+TDVDDLP DTF+KDSG ++ E KL+V+YI+
Sbjct: 18  RVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDDLPQDTFTKDSGKTLTECKLKVSYIS 77

Query: 473 PTSPDGSSEEDGLKNSAQKLGTSSSQALEQLKEERDVAARQTRMLQQELDMLKRQRNRRG 294
           P++   SSE      +  + G+ +   +++LKEERD A +QT+ LQ EL+M+KR+RN+  
Sbjct: 78  PSTTQRSSESGA--TNGDRNGSETISTIQRLKEERDAAVKQTQQLQHELEMVKRRRNQ-- 133

Query: 293 GGDPGFSFTFAVFVGLLGLLLGFLLKLLFSSPS 195
             + G S   A  VGL+GL++GF+LKL  +SP+
Sbjct: 134 -SNSGLSLKLAALVGLIGLIIGFILKLTLASPT 165

>gb|AAK43500.1|AC020666_10 putative membrane protein [Oryza sativa (japonica cultivar-group)]
          Length = 233

 Score =  149 bits (377), Expect = 2e-35
 Identities = 77/167 (46%), Positives = 115/167 (68%), Gaps = 14/167 (8%)
 Frame = -3

Query: 650 VTLQAQREFPPDMQCKDKFLLQSTIVNPNTDVDDLPPDTFSKDSGNSIEELKLRVAYITP 471
           +TLQAQ+E+PPDMQCKDKFL+QST V  +TD+D++PP+TF+K+    IEE+KL+V Y   
Sbjct: 70  ITLQAQKEYPPDMQCKDKFLIQSTKVAASTDMDEIPPNTFNKEVDKVIEEMKLKVVY--- 126

Query: 470 TSPDGSSEEDG---LKNSAQKLGT-----------SSSQALEQLKEERDVAARQTRMLQQ 333
           T P GSS++ G   L + + KLG+              Q +++LK+ERD   +Q + +Q+
Sbjct: 127 TVPSGSSDDSGITSLGSRSFKLGSDDLTMLKNASIEKIQTIQRLKDERDTTLQQNQQMQR 186

Query: 332 ELDMLKRQRNRRGGGDPGFSFTFAVFVGLLGLLLGFLLKLLFSSPSS 192
           ELD+++R+R+R+   D GFS TFA F GL+G+L+G L+ L+F  P +
Sbjct: 187 ELDVIRRRRSRK--SDAGFSLTFAAFAGLIGVLIGLLMSLIFPRPQA 231

>emb|CAC39038.1| putative vesicle-associated membrane protein (VAMP) [Oryza sativa]
          Length = 233

 Score =  149 bits (376), Expect = 3e-35
 Identities = 78/165 (47%), Positives = 113/165 (68%), Gaps = 14/165 (8%)
 Frame = -3

Query: 650 VTLQAQREFPPDMQCKDKFLLQSTIVNPNTDVDDLPPDTFSKDSGNSIEELKLRVAYITP 471
           +TLQAQ+++P DMQCKDKFL+QST V  +TD+D++PPDTF+K+    IEE+KL+V Y   
Sbjct: 70  ITLQAQKDYPQDMQCKDKFLIQSTRVAASTDMDEIPPDTFNKEVDKVIEEIKLKVVY--- 126

Query: 470 TSPDGSSEEDGLKNSAQKLGTSSS--------------QALEQLKEERDVAARQTRMLQQ 333
           T P GSS++ G+ +   +   S S              Q +++LK+ERD   +Q + +Q+
Sbjct: 127 TVPSGSSDDSGITSLGSRSFKSLSDDFTMLKNASIEEIQTIQRLKDERDNMLQQNQQMQR 186

Query: 332 ELDMLKRQRNRRGGGDPGFSFTFAVFVGLLGLLLGFLLKLLFSSP 198
           EL M++R+R+R+   D GFS TFA FVGL+GLL+G L+ L+FSSP
Sbjct: 187 ELVMIRRRRSRK--SDTGFSLTFAAFVGLIGLLVGLLMSLIFSSP 229

>pir||JC7234 27k vesicle-associated membrane protein-associated protein -
           curled-leaved tobacco gi|6688926|emb|CAB65313.1| VAP27
           [Nicotiana plumbaginifolia]
          Length = 240

 Score =  117 bits (293), Expect = 1e-25
 Identities = 68/171 (39%), Positives = 99/171 (57%), Gaps = 26/171 (15%)
 Frame = -3

Query: 650 VTLQAQREFPPDMQCKDKFLLQSTIVNPNTDVDDLPPDTFSKDSGNSIEELKLRVAYITP 471
           VT+QAQ+E P DMQCKDKFLLQS + +P     D+ P+ F+K+ GN +E+ KLRV Y+ P
Sbjct: 71  VTMQAQKEAPADMQCKDKFLLQSVVASPGATAKDITPEMFNKEEGNHVEDCKLRVIYVPP 130

Query: 470 --------------TSPDGSSEEDGLKNSA-----------QKLGTSSSQAL-EQLKEER 369
                         +SP  S  E+G  N++           Q+  +S ++AL  +L EE+
Sbjct: 131 QQPPSPVQEGSEEGSSPRASVSENGAVNTSEFNNISRAYNEQQDSSSETKALISKLTEEK 190

Query: 368 DVAARQTRMLQQELDMLKRQRNRRGGGDPGFSFTFAVFVGLLGLLLGFLLK 216
               +Q   LQQEL +L+R+RN   GG P     + + +GLLG++LG+LLK
Sbjct: 191 ISVIQQNNKLQQELALLRRERNSSRGGIPTI---YVLIIGLLGIILGYLLK 238

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 557,082,158
Number of Sequences: 1393205
Number of extensions: 11717532
Number of successful extensions: 49511
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 45707
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 49279
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 28144814643
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD007h08_f AV770492 1 517 2 MWM017f05_f AV764873 80 674 3 GNf029c04 BP069451 80 573 4 MFB035f02_f BP036582 92 360 5 MFB050e07_f BP037630 102 689

---