>KMC003231A_C01 KMC003231A_c01
gttagagaaatataaaatcaataaatcaagcaaatattctttcatggactattttgacat
ggtcctccttatACATACCCTGTTATCACTTTTATTAAGTGAAAGTGCGGGTAAATAAAC
ATATACTCTATTAATAGCTGACCAAAACTTGTAACGGTCACATTACAGGAGGAAAGAATG
ATAGACTAATTTAGAAAATCTAAGTTTCAGGATTACAACATTAAAAAATGACACCAGAGC
CGAAACTAAATGCTTAAATTTGCCTTTATTGTGATGAATGTCTACAAGAGATATTTTACA
CAAACATACAACTACATGAATCAGGGGCCTCATGCTACAAACTTGACGTGCAAAATCCAT
GAGTGATTGATTATGCATAATCCATCTACTCATTCTTGCATTCTGAAAGGTAGGATCGCG
CCAGCAGTAAGCACTTATCTTTTTTCCTTTTGTACTCGTTGTATCGAAGGAAAAAATAAC
CAAGGTAATCGAACTCGATGGGGGACATCTTTGCCTGAATTATCCCCCACAAAGCCCAGA
AAAGGTGTGACGCCAGTGCATAAGTGTTAGCTTCAACAATACAAAGGTTTCTAAGGATCT
TATCTCAGCACAGCGTCATGATGGTCG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003231A_C01 KMC003231A_c01
         (627 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM65218.1| putative choline kinase [Arabidopsis thaliana]          88  8e-17
ref|NP_180251.1| putative choline kinase; protein id: At2g26830....    88  8e-17
sp|P54352|EAS_DROME Ethanolamine kinase (EK) (Easily shocked pro...    70  3e-11
ref|NP_727941.1| easily shocked CG3525-PD gi|22832340|gb|AAN0938...    70  3e-11
ref|NP_788914.1| easily shocked CG3525-PE gi|28381628|gb|AAO4166...    70  3e-11

>gb|AAM65218.1| putative choline kinase [Arabidopsis thaliana]
          Length = 374

 Score = 88.2 bits (217), Expect = 8e-17
 Identities = 39/58 (67%), Positives = 47/58 (80%)
 Frame = -3

Query: 577 VEANTYALASHLFWALWGIIQAKMSPIEFDYLGYFFLRYNEYKRKKDKCLLLARSYLS 404
           VE + Y LASHL+WA+W IIQA+MSPIEF+YLGYFFLRYNEYK++K     L  S+LS
Sbjct: 314 VETDAYKLASHLYWAIWAIIQARMSPIEFEYLGYFFLRYNEYKKQKPLTFSLVTSHLS 371

>ref|NP_180251.1| putative choline kinase; protein id: At2g26830.1, supported by
           cDNA: 37620. [Arabidopsis thaliana]
           gi|7485395|pir||T02652 probable choline kinase At2g26830
           [imported] - Arabidopsis thaliana
           gi|3426043|gb|AAC32242.1| putative choline kinase
           [Arabidopsis thaliana]
          Length = 374

 Score = 88.2 bits (217), Expect = 8e-17
 Identities = 39/58 (67%), Positives = 47/58 (80%)
 Frame = -3

Query: 577 VEANTYALASHLFWALWGIIQAKMSPIEFDYLGYFFLRYNEYKRKKDKCLLLARSYLS 404
           VE + Y LASHL+WA+W IIQA+MSPIEF+YLGYFFLRYNEYK++K     L  S+LS
Sbjct: 314 VETDAYKLASHLYWAIWAIIQARMSPIEFEYLGYFFLRYNEYKKQKPLTFSLVTSHLS 371

>sp|P54352|EAS_DROME Ethanolamine kinase (EK) (Easily shocked protein)
           gi|532126|gb|AAC37209.1| ethanolamine kinase
          Length = 517

 Score = 69.7 bits (169), Expect = 3e-11
 Identities = 30/55 (54%), Positives = 41/55 (74%)
 Frame = -3

Query: 586 LCIVEANTYALASHLFWALWGIIQAKMSPIEFDYLGYFFLRYNEYKRKKDKCLLL 422
           L  V+ N +ALASH+FW +W ++QA+ S I+FDY+GY FLRYNEY  +K + L L
Sbjct: 456 LLYVQVNQFALASHIFWTVWSLLQAEHSTIDFDYVGYAFLRYNEYLARKVEFLSL 510

>ref|NP_727941.1| easily shocked CG3525-PD gi|22832340|gb|AAN09386.1| CG3525-PD
           [Drosophila melanogaster]
          Length = 474

 Score = 69.7 bits (169), Expect = 3e-11
 Identities = 30/55 (54%), Positives = 41/55 (74%)
 Frame = -3

Query: 586 LCIVEANTYALASHLFWALWGIIQAKMSPIEFDYLGYFFLRYNEYKRKKDKCLLL 422
           L  V+ N +ALASH+FW +W ++QA+ S I+FDY+GY FLRYNEY  +K + L L
Sbjct: 413 LLYVQVNQFALASHIFWTVWSLLQAEHSTIDFDYVGYAFLRYNEYLARKVEFLSL 467

>ref|NP_788914.1| easily shocked CG3525-PE gi|28381628|gb|AAO41661.1| CG3525-PE
           [Drosophila melanogaster]
          Length = 518

 Score = 69.7 bits (169), Expect = 3e-11
 Identities = 30/55 (54%), Positives = 41/55 (74%)
 Frame = -3

Query: 586 LCIVEANTYALASHLFWALWGIIQAKMSPIEFDYLGYFFLRYNEYKRKKDKCLLL 422
           L  V+ N +ALASH+FW +W ++QA+ S I+FDY+GY FLRYNEY  +K + L L
Sbjct: 457 LLYVQVNQFALASHIFWTVWSLLQAEHSTIDFDYVGYAFLRYNEYLARKVEFLSL 511

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 513,272,077
Number of Sequences: 1393205
Number of extensions: 10555169
Number of successful extensions: 19987
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 19531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19980
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25586195130
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF045g04_f BP030686 1 574 2 GNf028g03 BP069410 73 562 3 MR037f04_f BP078876 113 422 4 MPD034b10_f AV772311 114 590 5 GNf069d01 BP072475 163 639

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