>KMC003216A_C01 KMC003216A_c01
gagGAAATTTCAACCGCACTTTGAATTATTAACAACTAAAGATGAATACAGATCTTGAAT
TGCTTTCAAGCTATGGTACATTGTTATTTTTGAGAAAAACAAAATTAAATTAAGATTCTT
ATCTAAACATATCAAATTTAAATAACAATTACATTAATTAGGTGCCAACACAATACAAAA
CACCAACAACAATTCTGAAGGGTTAAACCTCTAAGCTATGCTTCCACTGTTGTTTCAACT
GCAACTTTATACTTGACAATTTCACGCTTCTCATCAACCTCCCATAGCTCTGGAAATGCC
TCCCTGATATTATGGATACTCTCTAATGCATGATCCACTCCAGTACTACGAACAGATGTA
CCCACCGCGACTGTGTGGAGGCCCAAGCGCTTGGCTGTCTGTAAATTGCGGATACTGTCC
TCAAAGAACAATGTTCTCTGAGGGTCAATGTCAGCCAACTGGAAAGCCTTTTCAAATGCA
TCTTCAAAGGGTTTACAGACAACTGGTGTCCTAGGAAGCACCTTATCATAATCAGTCCCT
CTCGTGTACTCGCAAAA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003216A_C01 KMC003216A_c01
         (557 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_195843.1| putative protein; protein id: At5g02230.1, supp...   132  3e-30
gb|AAM65114.1| unknown [Arabidopsis thaliana]                         132  3e-30
emb|CAB85628.1| putative ripening-related protein [Vitis vinifera]    131  5e-30
ref|NP_200756.1| putative ripening-related protein - like; prote...   126  2e-28
gb|AAM67205.1| putative ripening-related protein-like [Arabidops...   124  8e-28

>ref|NP_195843.1| putative protein; protein id: At5g02230.1, supported by cDNA:
           36724. [Arabidopsis thaliana] gi|11358409|pir||T48244
           hypothetical protein T7H20.280 - Arabidopsis thaliana
           gi|7340697|emb|CAB82996.1| putative protein [Arabidopsis
           thaliana]
          Length = 280

 Score =  132 bits (332), Expect = 3e-30
 Identities = 65/102 (63%), Positives = 79/102 (76%), Gaps = 2/102 (1%)
 Frame = -1

Query: 518 LPRTPVVCKPFEDAFEKAFQLADIDPQRTLFFEDSIRNLQTAKRLGLHTVAVGTSVRSTG 339
           LP+TPVVCKP E A EKA ++A+IDP RTLFFEDS+RN+Q  KR+GL+TV VG S +  G
Sbjct: 179 LPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKG 238

Query: 338 VDHALESIHNIREAFPELWEVDEKREIVKY--KVAVETTVEA 219
            D+ALE+IHN++EA PELWE D K   V Y  KVAVET+V A
Sbjct: 239 ADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSVRA 280

>gb|AAM65114.1| unknown [Arabidopsis thaliana]
          Length = 280

 Score =  132 bits (332), Expect = 3e-30
 Identities = 65/102 (63%), Positives = 79/102 (76%), Gaps = 2/102 (1%)
 Frame = -1

Query: 518 LPRTPVVCKPFEDAFEKAFQLADIDPQRTLFFEDSIRNLQTAKRLGLHTVAVGTSVRSTG 339
           LP+TPVVCKP E A EKA ++A+IDP RTLFFEDS+RN+Q  KR+GL+TV VG S +  G
Sbjct: 179 LPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKG 238

Query: 338 VDHALESIHNIREAFPELWEVDEKREIVKY--KVAVETTVEA 219
            D+ALE+IHN++EA PELWE D K   V Y  KVAVET+V A
Sbjct: 239 ADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSVRA 280

>emb|CAB85628.1| putative ripening-related protein [Vitis vinifera]
          Length = 301

 Score =  131 bits (330), Expect = 5e-30
 Identities = 66/101 (65%), Positives = 80/101 (78%), Gaps = 1/101 (0%)
 Frame = -1

Query: 518 LPRTPVVCKPFEDAFEKAFQLADIDPQRTLFFEDSIRNLQTAKRLGLHTVAVGTSVRSTG 339
           LP+TP+VCKP E A E+A ++A+I+PQRTLFFEDS RN+Q+ KR+GLHTV VGTS R  G
Sbjct: 201 LPKTPIVCKPSEAAIERALRIANINPQRTLFFEDSARNIQSGKRVGLHTVLVGTSQRIKG 260

Query: 338 VDHALESIHNIREAFPELWEVDEKREI-VKYKVAVETTVEA 219
            D ALESIHN+REA PELWE D+K E+    +VAVET V A
Sbjct: 261 ADFALESIHNMREALPELWEGDKKSEVGYAGQVAVETPVTA 301

>ref|NP_200756.1| putative ripening-related protein - like; protein id: At5g59480.1,
           supported by cDNA: 99078., supported by cDNA:
           gi_17979376, supported by cDNA: gi_20465780 [Arabidopsis
           thaliana] gi|8885553|dbj|BAA97483.1| ripening-related
           protein-like; hydrolase-like [Arabidopsis thaliana]
           gi|17979377|gb|AAL49914.1| putative ripening-related
           protein [Arabidopsis thaliana]
           gi|20465781|gb|AAM20379.1| putative ripening protein
           [Arabidopsis thaliana]
          Length = 282

 Score =  126 bits (317), Expect = 2e-28
 Identities = 65/109 (59%), Positives = 82/109 (74%), Gaps = 3/109 (2%)
 Frame = -1

Query: 548 YTRGTDYDKVLPRTPVVCKPFEDAFEKAFQLADIDPQRTLFFEDSIRNLQTAKRLGLHTV 369
           Y    D    LP+T VVCKP E AFE+ F++A+I+P++TLFF+DSIRN+QT KR+GLHTV
Sbjct: 172 YMANPDSSIELPKTSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTV 231

Query: 368 AVGTSVRSTGVDHALESIHNIREAFPELWEV--DEKREI-VKYKVAVET 231
            VGTS R  GVD ALE IHNIREA P+LW+   D+ +EI  + KVA+ET
Sbjct: 232 WVGTSHREEGVDIALEHIHNIREALPQLWDAVDDKAKEIRTRQKVAIET 280

>gb|AAM67205.1| putative ripening-related protein-like [Arabidopsis thaliana]
          Length = 282

 Score =  124 bits (311), Expect = 8e-28
 Identities = 65/109 (59%), Positives = 82/109 (74%), Gaps = 3/109 (2%)
 Frame = -1

Query: 548 YTRGTDYDKVLPRTPVVCKPFEDAFEKAFQLADIDPQRTLFFEDSIRNLQTAKRLGLHTV 369
           Y    D    LP+T VVCKP E AFE+ F++A+I+P++TLFF+DSIRN+QT KR+GLHTV
Sbjct: 172 YMANPDSSIELPKTSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTV 231

Query: 368 AVGTSVRSTGVDHALESIHNIREAFPELWE-VDEKREIV--KYKVAVET 231
            VGTS R  GVD ALE IHNIREA  +LW+ VD+K E +  + KVA+ET
Sbjct: 232 WVGTSHREEGVDIALEHIHNIREALLQLWDAVDDKAEEIRTRQKVAIET 280

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 453,988,573
Number of Sequences: 1393205
Number of extensions: 9644341
Number of successful extensions: 26423
Number of sequences better than 10.0: 159
Number of HSP's better than 10.0 without gapping: 25508
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26411
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR029e06_f BP078245 1 402 2 MR067e05_f BP081159 4 344 3 GNf027f12 BP069333 16 424 4 GNf070h11 BP072586 32 507 5 MFB024f10_f BP035751 63 561

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