>KMC003169A_C01 KMC003169A_c01
cttACCCCAAAAGCGAGTGCATATATATATGATTAAAGGCACATAATCCGCAAGTCTAAA
CTTGACATATATATATTGAAGTACATAAATTAAAATAGAGAACTATATATATATGAATAA
TAATGTGATGCTTTTGGCACCTCGATCACAAAGATGATGGCATCCATGTATATTCACTCT
CACTGCCACTTTTCACCCCACTATCAGTCTACTACCAGTACAGCACTGTCTGCTTTTATA
GCAATCAGATATATGCATCATATCCCAGTAATTTTAAACTTTTGCTTCTGCCACACCAAA
CAGGATCCAAAGACAAACAGTGCTCATGTACCATCTTTCAGATTAATAATCAACAAAAAA
AAAACAAGAACCCTAATAAATACCATATTGATTCTAAAAGCATTGAGATTAAGAGGAGAG
AGAGTGGATTATAATTTTCCCCTAATGAGTGGCACCTGCTGGAGGTGGCATCATGGATGG
TGGTGG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003169A_C01 KMC003169A_c01
         (486 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_703875.1| hypothetical protein [Plasmodium falciparum 3D7...    35  0.57
sp||Q9Y6V0_1 [Segment 1 of 2] Piccolo protein (Aczonin)                32  2.9
emb|CAB60727.1| aczonin [Homo sapiens]                                 32  2.9
ref|NP_752521.1| Hypothetical protein ybaN [Escherichia coli CFT...    32  4.9
gb|EAA21185.1| hypothetical protein [Plasmodium yoelii yoelii]         31  6.4

>ref|NP_703875.1| hypothetical protein [Plasmodium falciparum 3D7]
           gi|23498536|emb|CAD50487.1| hypothetical protein
           [Plasmodium falciparum 3D7]
          Length = 1633

 Score = 34.7 bits (78), Expect = 0.57
 Identities = 16/53 (30%), Positives = 32/53 (60%), Gaps = 2/53 (3%)
 Frame = -2

Query: 170 YMDAIIFVIEVPKASHYYSYIYSSLF*FMYFNIYMSSLDLRIMC--L*SYIYA 18
           Y+D +  +  + K + Y  Y+Y+ +F +   NIY+ +++++ +C  L SY YA
Sbjct: 727 YLDIVNIIYSLTKLNEYDEYLYNDIF-YNAINIYIHNMNIKNLCIILWSYTYA 778

>sp||Q9Y6V0_1 [Segment 1 of 2] Piccolo protein (Aczonin)
          Length = 1010

 Score = 32.3 bits (72), Expect = 2.9
 Identities = 14/44 (31%), Positives = 21/44 (46%)
 Frame = -3

Query: 163 MPSSL*SRCQKHHIIIHIYIVLYFNLCTSIYICQV*TCGLCAFN 32
           +P ++   C    +++H+     FN CT    CQ   C LC FN
Sbjct: 494 LPKTICPLCNTTELLLHVPEKANFNTCTE---CQTTVCSLCGFN 534

>emb|CAB60727.1| aczonin [Homo sapiens]
          Length = 759

 Score = 32.3 bits (72), Expect = 2.9
 Identities = 14/44 (31%), Positives = 21/44 (46%)
 Frame = -3

Query: 163 MPSSL*SRCQKHHIIIHIYIVLYFNLCTSIYICQV*TCGLCAFN 32
           +P ++   C    +++H+     FN CT    CQ   C LC FN
Sbjct: 494 LPKTICPLCNTTELLLHVPEKANFNTCTE---CQTTVCSLCGFN 534

>ref|NP_752521.1| Hypothetical protein ybaN [Escherichia coli CFT073]
           gi|26106880|gb|AAN79065.1|AE016756_248 Hypothetical
           protein ybaN [Escherichia coli CFT073]
          Length = 125

 Score = 31.6 bits (70), Expect = 4.9
 Identities = 18/51 (35%), Positives = 27/51 (52%)
 Frame = -1

Query: 159 HHLCDRGAKSITLLFIYI*FSILIYVLQYIYVKFRLADYVPLIIYICTRFW 7
           HH   RGAK   +L I I F+I ++ +Q  +V+  L     L+I  C  F+
Sbjct: 66  HHAMPRGAKPRAILLILITFTISLWFVQMPWVRIML-----LVILACLLFY 111

>gb|EAA21185.1| hypothetical protein [Plasmodium yoelii yoelii]
          Length = 657

 Score = 31.2 bits (69), Expect = 6.4
 Identities = 16/49 (32%), Positives = 28/49 (56%), Gaps = 2/49 (4%)
 Frame = -2

Query: 158 IIFVIEVPKASHYYSY--IYSSLF*FMYFNIYMSSLDLRIMCL*SYIYA 18
           + F++ + K   Y  +  +YS LF  +  NIYM + ++ I CL  Y+Y+
Sbjct: 575 LYFLLNLNKNEKYIKHAELYSFLFSILKNNIYMINKEVAIQCLYIYLYS 623

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 426,531,882
Number of Sequences: 1393205
Number of extensions: 8890207
Number of successful extensions: 26243
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 23853
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25957
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13980339936
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf024a09 BP069075 1 400 2 GNf029a09 BP069436 4 424 3 GNf095f05 BP074423 4 408 4 GNf087h06 BP073818 4 388 5 GNf061d06 BP071899 4 490 6 GNf089e08 BP073946 87 477 7 GNf095d10 BP074407 87 477

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