Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003157A_C01 KMC003157A_c01
(1043 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_194858.1| 20S proteasome beta subunit A (PBA1); protein i... 417 e-115
gb|AAM64316.1| multicatalytic endopeptidase complex, proteasome ... 403 e-111
sp|P93395|PSB6_TOBAC Proteasome subunit beta type 6 precursor (P... 391 e-107
dbj|BAA96834.1| beta 1 subunit of 20S proteasome [Oryza sativa (... 374 e-102
pir||JE0101 proteasome subunit 1 - slime mold (Dictyostelium dis... 228 2e-58
>ref|NP_194858.1| 20S proteasome beta subunit A (PBA1); protein id: At4g31300.1,
supported by cDNA: 229., supported by cDNA: gi_15450504,
supported by cDNA: gi_16323317, supported by cDNA:
gi_3421098 [Arabidopsis thaliana] gi|7435895|pir||T04497
C 3.4.25.1 proteasome endopeptidase complex () chain PBA1
[imported] - Arabidopsis thaliana
gi|2511594|emb|CAA74028.1| multicatalytic endopeptidase
complex, proteasome precursor, beta subunit [Arabidopsis
thaliana] gi|2827525|emb|CAA16533.1| multicatalytic
endopeptidase complex, proteasome precursor, beta subunit
[Arabidopsis thaliana] gi|3421099|gb|AAC32065.1| 20S
proteasome subunit PBA1 [Arabidopsis thaliana]
gi|7270032|emb|CAB79848.1| multicatalytic endopeptidase
complex, proteasome precursor, beta subunit [Arabidopsis
thaliana] gi|15450505|gb|AAK96545.1| AT4g31300/F8F16_120
[Arabidopsis thaliana] gi|16323318|gb|AAL15414.1|
AT4g31300/F8F16_120 [Arabidopsis thaliana]
Length = 233
Score = 417 bits (1072), Expect = e-115
Identities = 207/233 (88%), Positives = 220/233 (93%), Gaps = 2/233 (0%)
Frame = -1
Query: 1001 MDQNFDLSAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRS 822
MD N D APHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRS
Sbjct: 1 MDLNLD--APHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRS 58
Query: 821 GSAADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLSYNNKNFLETGLIVGGWDKY 642
GSAADSQVVSDYVRYFLHQHTIQ GQPATVKV+ANL+R+L+YNNKN L+TGLIVGGWDKY
Sbjct: 59 GSAADSQVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQTGLIVGGWDKY 118
Query: 641 EGGQIFGVPLGGTIVQQPFAIGGSGSSYLYGFFDQAWKDGMTKDEAEDLVKKAVSLAIAR 462
EGG+I+G+PLGGT+V+QPFAIGGSGSSYLYGFFDQAWKD MTK+EAE LV KAVSLAIAR
Sbjct: 119 EGGKIYGIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIAR 178
Query: 461 DGASGGVVRTVIINSEGVTRNFYPGDQLPLWHEELEPQNSLLDILGA--PEPM 309
DGASGGVVRTVIINSEGVTRNFYPGD+L LWHEELEPQNSLLDIL A PEPM
Sbjct: 179 DGASGGVVRTVIINSEGVTRNFYPGDKLQLWHEELEPQNSLLDILNAAGPEPM 231
>gb|AAM64316.1| multicatalytic endopeptidase complex, proteasome precursor, beta
subunit [Arabidopsis thaliana]
Length = 223
Score = 403 bits (1036), Expect = e-111
Identities = 198/221 (89%), Positives = 212/221 (95%), Gaps = 2/221 (0%)
Frame = -1
Query: 965 MGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDY 786
MGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGSAADSQVVSDY
Sbjct: 1 MGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDY 60
Query: 785 VRYFLHQHTIQLGQPATVKVAANLVRLLSYNNKNFLETGLIVGGWDKYEGGQIFGVPLGG 606
VR+FLHQHTIQ GQPATVKV+ANL+R+L+YNNKN L+TGLIVGGWDKYEGG+I+G+PLGG
Sbjct: 61 VRHFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQTGLIVGGWDKYEGGKIYGIPLGG 120
Query: 605 TIVQQPFAIGGSGSSYLYGFFDQAWKDGMTKDEAEDLVKKAVSLAIARDGASGGVVRTVI 426
T+V+QPFAIGGSGSSYLYGFFDQAWKD MTK+EAE LV KAVSLAIARDGASGGVVRTVI
Sbjct: 121 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 180
Query: 425 INSEGVTRNFYPGDQLPLWHEELEPQNSLLDILGA--PEPM 309
INSEGVTRNFYPGD+L LWHEELEPQNSLLDIL A PEPM
Sbjct: 181 INSEGVTRNFYPGDKLQLWHEELEPQNSLLDILNAAGPEPM 221
>sp|P93395|PSB6_TOBAC Proteasome subunit beta type 6 precursor (Proteasome delta chain)
(Tobacco cryptogein-induced protein 7) (tcI 7)
gi|7435893|pir||T03985 C 3.4.25.1 proteasome
endopeptidase complex () delta chain - common tobacco
gi|1743356|emb|CAA70699.1| proteasome delta subunit
[Nicotiana tabacum]
Length = 234
Score = 391 bits (1004), Expect = e-107
Identities = 188/229 (82%), Positives = 209/229 (91%)
Frame = -1
Query: 995 QNFDLSAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGS 816
+N D+ PHSMGTTIIGVTYNGGVVLGADSRTSTG+YVANRASDKITQLTDNVYVCRSGS
Sbjct: 2 ENTDVDQPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGS 61
Query: 815 AADSQVVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLSYNNKNFLETGLIVGGWDKYEG 636
AADSQ+VSDYVRYFLHQHTIQLGQPATVKVAANL RLLSYNNK+ L+TG+I+GGWDKYEG
Sbjct: 62 AADSQIVSDYVRYFLHQHTIQLGQPATVKVAANLTRLLSYNNKDRLQTGMIIGGWDKYEG 121
Query: 635 GQIFGVPLGGTIVQQPFAIGGSGSSYLYGFFDQAWKDGMTKDEAEDLVKKAVSLAIARDG 456
G+I+G+P GGT+++QPFAIGGSGSSYLYGFFDQAWK+GMT++EAE LV AVSLAIARDG
Sbjct: 122 GKIYGIPPGGTVLEQPFAIGGSGSSYLYGFFDQAWKEGMTQEEAEKLVVTAVSLAIARDG 181
Query: 455 ASGGVVRTVIINSEGVTRNFYPGDQLPLWHEELEPQNSLLDILGAPEPM 309
ASGGVVRTV IN +G TR FY GD L LWHEELEP NSLLD++ A P+
Sbjct: 182 ASGGVVRTVTINKDGATRKFYSGDSLQLWHEELEPVNSLLDVVFASSPV 230
>dbj|BAA96834.1| beta 1 subunit of 20S proteasome [Oryza sativa (japonica
cultivar-group)]
Length = 246
Score = 374 bits (960), Expect = e-102
Identities = 181/226 (80%), Positives = 203/226 (89%), Gaps = 2/226 (0%)
Frame = -1
Query: 980 SAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQ 801
S H MGTTI+GV Y+GGVVL ADSRTSTG+YVANRASDKITQLTDNVY+CRSGSAAD+Q
Sbjct: 19 SGEHRMGTTIVGVCYDGGVVLAADSRTSTGMYVANRASDKITQLTDNVYICRSGSAADTQ 78
Query: 800 VVSDYVRYFLHQHTIQLGQPATVKVAANLVRLLSYNNKNFLETGLIVGGWDKYEGGQIFG 621
V+SDYVRYFLHQHTIQLGQPATVKVAANL+RLL+Y NKN L+ G+IVGGWDKYEGGQIF
Sbjct: 79 VISDYVRYFLHQHTIQLGQPATVKVAANLIRLLAYQNKNMLQAGMIVGGWDKYEGGQIFS 138
Query: 620 VPLGGTIVQQPFAIGGSGSSYLYGFFDQAWKDGMTKDEAEDLVKKAVSLAIARDGASGGV 441
VPLGGTI++QPFAIGGSGSSYLYG D WK+GM+++EAE V K VSLAIARDGASGGV
Sbjct: 139 VPLGGTILRQPFAIGGSGSSYLYGLLDHEWKEGMSQEEAEKFVVKVVSLAIARDGASGGV 198
Query: 440 VRTVIINSEGVTRNFYPGDQLPLWHEELEPQNSLLDIL--GAPEPM 309
VRTV IN++GV+R F+PGD+L LWHEELEPQNSLLDIL G P+PM
Sbjct: 199 VRTVTINADGVSRKFHPGDKLQLWHEELEPQNSLLDILAAGNPDPM 244
>pir||JE0101 proteasome subunit 1 - slime mold (Dictyostelium discoideum)
gi|3116216|dbj|BAA25923.1| proteasome subunit
[Dictyostelium discoideum]
Length = 213
Score = 228 bits (580), Expect = 2e-58
Identities = 107/201 (53%), Positives = 150/201 (74%)
Frame = -1
Query: 965 MGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDY 786
+GT+I+ V Y+GGV++GADSRT+TG Y+ANR ++KIT + + +Y CRSGSAAD+Q +SDY
Sbjct: 13 LGTSIMAVEYDGGVIMGADSRTTTGAYIANRVTNKITPIHERIYCCRSGSAADTQAISDY 72
Query: 785 VRYFLHQHTIQLGQPATVKVAANLVRLLSYNNKNFLETGLIVGGWDKYEGGQIFGVPLGG 606
VRY+L HT +L VK AA L +LL Y+NKN L G+IV GWDK++ G ++ + LGG
Sbjct: 73 VRYYLEMHTSELCDEPDVKTAACLFQLLCYSNKNNLMAGIIVAGWDKHQ-GSVYNISLGG 131
Query: 605 TIVQQPFAIGGSGSSYLYGFFDQAWKDGMTKDEAEDLVKKAVSLAIARDGASGGVVRTVI 426
++V+QPFAIGGSGS+Y+YG+ D +K MTKD + V+ +++LA+ RDG+SGGV+R I
Sbjct: 132 SMVKQPFAIGGSGSTYIYGYCDSKFKPKMTKDRCIEFVQNSLALAMFRDGSSGGVIRLCI 191
Query: 425 INSEGVTRNFYPGDQLPLWHE 363
I+ GV R PG+ LP + E
Sbjct: 192 IDKNGVERKMIPGNNLPRFWE 212
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 954,144,570
Number of Sequences: 1393205
Number of extensions: 23507831
Number of successful extensions: 92568
Number of sequences better than 10.0: 513
Number of HSP's better than 10.0 without gapping: 78279
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91283
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 61532797421
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
