>KMC003152A_C01 KMC003152A_c01
ccccaaaatttcgatgcagacacaagactaatgcttggcacagagcctcagtctggccca
gagccatccaatgttacaaaTTACTCACTTGGCACTACAGTCTGGGCTCCAGGATACAGG
GATAACATCAGAGAACCGAATAAGCAATCCTAAAAGATGTAGATATTTTGGCTGTAAAAA
GGGAGCTCGCGGTGCTTCAGGACTTGGTATTGGACATGGTGGTGGACAGAGATGTCAGAA
ACCAGGATGCAACAAGGGTGCTGAGAGCCGTACTGCTTACTGTAAGGCCCACGGTGGGGG
GAGGAGGTGCAACCACTTAGGGTGTACTAAAAGTGCTGAGGGGAAGACAGATTATTGCAT
AGCACACGGTGGTGGCAAGCGATGTGGTTATCCAGATGGGTGCACGAAAGCTGCACGAGG
TAAGTCAGGACTTTGCATTAGACATGGAGGGGGTAAGAGATGCAGGATAGAAGGTTGCGC
CAGGAGTGCTGAAGGGCAGGCTGGCTTGTGCATCTCTCATGGGGGAGGACGCCGTTGTCA
GTACCTAGGATGCTCAAAGGGCGCGCAAGGGAGCACCATGTTTTGCAAGGCTCATGGAGG
CGGAAAGCGTTGTTCATTTGCAGGGTGCAGTAAAGGAGCTGAAGGAAGCACTCCACTGTG
CAAGGCACATGGTGGGGGGAAGCGTTGCCTTTACAATGGCGGTGGCATTTGCGGAAAAAG
CGTTCATGGAGGGACAAACTTCTGTGTTGCTCATGGTGGTGGAAAGAGGTGTGCTGTTTC
AGGCTGCACCAAGAGTGCTCGTGGCCGCACTGACTGTTGTGTTAGGCATGGTGGGGGAAA
ACGGTGCAAGTCTGAAGGCTGTGCTAAGAGTGCacagggtagcacagatttctgcaaggc
ccacggtggaggaaagcgatgtagctggggagatggaaagtgtgagaaatttg

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003152A_C01 KMC003152A_c01
         (953 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_201260.1| putative protein; protein id: At5g64550.1, supp...   533  e-150
ref|NP_196529.1| putative protein; protein id: At5g09670.1 [Arab...   516  e-145
dbj|BAB21076.1| P0501G01.5 [Oryza sativa (japonica cultivar-group)]   482  e-135
pir||H96665 protein F22C12.10 [imported] - Arabidopsis thaliana ...   469  e-131
ref|NP_176596.1| hypothetical protein; protein id: At1g64140.1 [...   469  e-131

>ref|NP_201260.1| putative protein; protein id: At5g64550.1, supported by cDNA:
            gi_20259337 [Arabidopsis thaliana]
            gi|10178058|dbj|BAB11422.1|
            emb|CAB89363.1~gene_id:MUB3.7~strong similarity to
            unknown protein [Arabidopsis thaliana]
            gi|20259338|gb|AAM13994.1| unknown protein [Arabidopsis
            thaliana] gi|23296960|gb|AAN13211.1| unknown protein
            [Arabidopsis thaliana]
          Length = 634

 Score =  533 bits (1374), Expect = e-150
 Identities = 233/277 (84%), Positives = 257/277 (92%), Gaps = 1/277 (0%)
 Frame = +2

Query: 125  TSENRISNPKRCRYFGCKKGARGASGLGIGHGGGQRCQKPGCNKGAESRTAYCKAHGGGR 304
            +S+ R+SNPK+C++ GC KGARGASGL IGHGGGQRCQK GCNKGAES+T +CKAHGGG+
Sbjct: 205  SSQQRMSNPKKCKFMGCVKGARGASGLCIGHGGGQRCQKLGCNKGAESKTTFCKAHGGGK 264

Query: 305  RCNHLGCTKSAEGKTDYCIAHGGGKRCGYPDGCTKAARGKSGLCIRHGGGKRCRIEGCAR 484
            RC HLGCTKSAEGKTD CI+HGGG+RCG+P+GC KAARGKSGLCI+HGGGKRCRIE C R
Sbjct: 265  RCQHLGCTKSAEGKTDLCISHGGGRRCGFPEGCAKAARGKSGLCIKHGGGKRCRIESCTR 324

Query: 485  SAEGQAGLCISHGGGRRCQYLGCSKGAQGSTMFCKAHGGGKRCSFAGCSKGAEGSTPLCK 664
            SAEGQAGLCISHGGGRRCQ  GC+KGAQGST +CKAHGGGKRC FAGC+KGAEGSTPLCK
Sbjct: 325  SAEGQAGLCISHGGGRRCQSSGCTKGAQGSTNYCKAHGGGKRCIFAGCTKGAEGSTPLCK 384

Query: 665  AHGGGKRCLYNGGGICGKSVHGGTNFCVAHGGGKRCAVSGCTKSARGRTDCCVRHGGGKR 844
            AHGGGKRC+++GGGIC KSVHGGT+FCVAHGGGKRC V+GCTKSARGRTDCCV+HGGGKR
Sbjct: 385  AHGGGKRCMFDGGGICPKSVHGGTSFCVAHGGGKRCVVAGCTKSARGRTDCCVKHGGGKR 444

Query: 845  CKSEGCAKSAQGSTDFCKAHGGGKRCSW-GDGKCEKF 952
            CKS+GC KSAQGSTDFCKAHGGGKRCSW GD KCEKF
Sbjct: 445  CKSDGCEKSAQGSTDFCKAHGGGKRCSWGGDWKCEKF 481

 Score = 43.5 bits (101), Expect = 0.005
 Identities = 19/50 (38%), Positives = 27/50 (54%)
 Frame = +2

Query: 773 AVSGCTKSARGRTDCCVRHGGGKRCKSEGCAKSAQGSTDFCKAHGGGKRC 922
           +VS  +  +   T    R    K+CK  GC K A+G++  C  HGGG+RC
Sbjct: 192 SVSAFSDRSASATSSQQRMSNPKKCKFMGCVKGARGASGLCIGHGGGQRC 241

>ref|NP_196529.1| putative protein; protein id: At5g09670.1 [Arabidopsis thaliana]
           gi|11357501|pir||T49931 hypothetical protein F17I14.140
           - Arabidopsis thaliana gi|7671422|emb|CAB89363.1|
           putative protein [Arabidopsis thaliana]
           gi|9758995|dbj|BAB09522.1|
           gb|AAF24563.1~gene_id:MTH16.9~strong similarity to
           unknown protein [Arabidopsis thaliana]
           gi|22530998|gb|AAM97003.1| putative protein [Arabidopsis
           thaliana]
          Length = 546

 Score =  516 bits (1328), Expect = e-145
 Identities = 226/275 (82%), Positives = 246/275 (89%), Gaps = 1/275 (0%)
 Frame = +2

Query: 131 ENRISNPKRCRYFGCKKGARGASGLGIGHGGGQRCQKPGCNKGAESRTAYCKAHGGGRRC 310
           + R SNP++C++ GC KGARGASGL I HGGGQRCQKPGCNKGAES+T +CK HGGG+RC
Sbjct: 147 QQRTSNPRKCKFMGCVKGARGASGLCISHGGGQRCQKPGCNKGAESKTTFCKTHGGGKRC 206

Query: 311 NHLGCTKSAEGKTDYCIAHGGGKRCGYPDGCTKAARGKSGLCIRHGGGKRCRIEGCARSA 490
            HLGCTKSAEGKTD+CI+HGGG+RC + +GC KAARG+SGLCI+HGGGKRC IE C RSA
Sbjct: 207 EHLGCTKSAEGKTDFCISHGGGRRCEFLEGCDKAARGRSGLCIKHGGGKRCNIEDCTRSA 266

Query: 491 EGQAGLCISHGGGRRCQYL-GCSKGAQGSTMFCKAHGGGKRCSFAGCSKGAEGSTPLCKA 667
           EGQAGLCISHGGG+RCQY  GC KGAQGST +CKAHGGGKRC F+GCSKGAEGSTPLCKA
Sbjct: 267 EGQAGLCISHGGGKRCQYFSGCEKGAQGSTNYCKAHGGGKRCIFSGCSKGAEGSTPLCKA 326

Query: 668 HGGGKRCLYNGGGICGKSVHGGTNFCVAHGGGKRCAVSGCTKSARGRTDCCVRHGGGKRC 847
           HGGGKRCL +GGGIC KSVHGGTNFCVAHGGGKRC V GCTKSARGRTD CV+HGGGKRC
Sbjct: 327 HGGGKRCLADGGGICSKSVHGGTNFCVAHGGGKRCVVVGCTKSARGRTDSCVKHGGGKRC 386

Query: 848 KSEGCAKSAQGSTDFCKAHGGGKRCSWGDGKCEKF 952
           K   C KSAQGSTDFCKAHGGGKRCSWGDGKCEKF
Sbjct: 387 KIIDCEKSAQGSTDFCKAHGGGKRCSWGDGKCEKF 421

 Score =  117 bits (294), Expect = 2e-25
 Identities = 55/102 (53%), Positives = 63/102 (60%), Gaps = 4/102 (3%)
 Frame = +2

Query: 152 KRCRYFG---CKKGARGASGLGIGHGGGQRCQKPGCNKGAESRTAYCKAHGGGRRCNHLG 322
           KRC   G   C K   G +   + HGGG+RC   GC K A  RT  C  HGGG+RC  + 
Sbjct: 331 KRCLADGGGICSKSVHGGTNFCVAHGGGKRCVVVGCTKSARGRTDSCVKHGGGKRCKIID 390

Query: 323 CTKSAEGKTDYCIAHGGGKRCGYPDG-CTKAARGKSGLCIRH 445
           C KSA+G TD+C AHGGGKRC + DG C K ARGKSGLC  H
Sbjct: 391 CEKSAQGSTDFCKAHGGGKRCSWGDGKCEKFARGKSGLCAAH 432

>dbj|BAB21076.1| P0501G01.5 [Oryza sativa (japonica cultivar-group)]
          Length = 646

 Score =  482 bits (1240), Expect = e-135
 Identities = 220/311 (70%), Positives = 248/311 (79%), Gaps = 12/311 (3%)
 Frame = +2

Query: 56   AQSHPMLQ---ITHLALQSGLQDTGITSENRISNPKRCRYFGCKKGARGASGLGIGHGGG 226
            A S P++    +T +          I  + R S  K C++ GC KGARGASG  I HGGG
Sbjct: 226  AMSSPVISSTLVTSMKSPVACTSGSINPQQRNSITKNCQFPGCVKGARGASGHCIAHGGG 285

Query: 227  QRCQKPGCNKGAESRTAYCKAHGGGRRCNHLGCTKSAEGKTDYCIAHGGGKRCGYPDGCT 406
            +RCQKPGC KGAE RT YCKAHGGGRRC  LGCTKSAEG+TD+CIAHGGG+RC + DGC+
Sbjct: 286  RRCQKPGCQKGAEGRTIYCKAHGGGRRCQFLGCTKSAEGRTDHCIAHGGGRRCSH-DGCS 344

Query: 407  KAARGKSGLCIRHGGGKRCRIEGCARSAEGQAGLCISHGGGRRCQYLGCSKGAQGSTMFC 586
            +AARGKSGLCIRHGGGKRC+ E C RSAEG +G CISHGGGRRCQ+  C+KGAQGST FC
Sbjct: 345  RAARGKSGLCIRHGGGKRCQKENCIRSAEGHSGFCISHGGGRRCQFPECTKGAQGSTKFC 404

Query: 587  KAHGGGKRCSFAGCSKGAEGSTPLCKAHGGGKRCLYNGGGICGKSVHGGTNFCVAHGGGK 766
            KAHGGGKRC+F+GC+KGAEGST  CK HGGGKRCL+ GGG+C KSVHGGT +CVAHGGGK
Sbjct: 405  KAHGGGKRCTFSGCNKGAEGSTLFCKGHGGGKRCLFQGGGVCPKSVHGGTQYCVAHGGGK 464

Query: 767  RCAVSGCTKSARGRTDCCVRHGGGKRCKSEGCAKSAQGSTDFCKAHGGGKRCSWGD---- 934
            RCA+SGCTKSARGRT+ CVRHGGGKRCK EGCAKSAQGSTDFCKAHGGGKRCSWG     
Sbjct: 465  RCAISGCTKSARGRTEYCVRHGGGKRCKFEGCAKSAQGSTDFCKAHGGGKRCSWGQVDLN 524

Query: 935  -----GKCEKF 952
                  +C+KF
Sbjct: 525  FGVGAPQCDKF 535

 Score =  217 bits (552), Expect = 2e-55
 Identities = 99/186 (53%), Positives = 118/186 (63%), Gaps = 13/186 (6%)
 Frame = +2

Query: 152 KRCRYFGCKKGARGASGLGIGHGGGQRCQKPGCNKGAESRTAYCKAHGGGRRCNHLGCTK 331
           KRC+   C + A G SG  I HGGG+RCQ P C KGA+  T +CKAHGGG+RC   GC K
Sbjct: 361 KRCQKENCIRSAEGHSGFCISHGGGRRCQFPECTKGAQGSTKFCKAHGGGKRCTFSGCNK 420

Query: 332 SAEGKTDYCIAHGGGKRCGYPDG--CTKAARGKSGLCIRHGGGKRCRIEGCARSAEGQAG 505
            AEG T +C  HGGGKRC +  G  C K+  G +  C+ HGGGKRC I GC +SA G+  
Sbjct: 421 GAEGSTLFCKGHGGGKRCLFQGGGVCPKSVHGGTQYCVAHGGGKRCAISGCTKSARGRTE 480

Query: 506 LCISHGGGRRCQYLGCSKGAQGSTMFCKAHGGGKRCSFAG-----------CSKGAEGST 652
            C+ HGGG+RC++ GC+K AQGST FCKAHGGGKRCS+             C K A   T
Sbjct: 481 YCVRHGGGKRCKFEGCAKSAQGSTDFCKAHGGGKRCSWGQVDLNFGVGAPQCDKFARSKT 540

Query: 653 PLCKAH 670
            LC AH
Sbjct: 541 GLCSAH 546

>pir||H96665 protein F22C12.10 [imported] - Arabidopsis thaliana
            gi|6692098|gb|AAF24563.1|AC007764_5 F22C12.10
            [Arabidopsis thaliana]
          Length = 646

 Score =  469 bits (1208), Expect = e-131
 Identities = 212/289 (73%), Positives = 238/289 (81%), Gaps = 5/289 (1%)
 Frame = +2

Query: 95   LQSGLQDTGITSE-----NRISNPKRCRYFGCKKGARGASGLGIGHGGGQRCQKPGCNKG 259
            + SG   +G++ +        S+ K C+  GC KGARGASG  I HGGG+RCQK GC+KG
Sbjct: 233  ISSGTCTSGLSQQLKPQLKNSSSSKLCQVEGCHKGARGASGRCISHGGGRRCQKHGCHKG 292

Query: 260  AESRTAYCKAHGGGRRCNHLGCTKSAEGKTDYCIAHGGGKRCGYPDGCTKAARGKSGLCI 439
            AE RT YCKAHGGGRRC  LGCTKSAEG+TD+CIAHGGG+RC + D CT+AARG+SGLCI
Sbjct: 293  AEGRTVYCKAHGGGRRCEFLGCTKSAEGRTDFCIAHGGGRRCSHED-CTRAARGRSGLCI 351

Query: 440  RHGGGKRCRIEGCARSAEGQAGLCISHGGGRRCQYLGCSKGAQGSTMFCKAHGGGKRCSF 619
            RHGGGKRC+ E C +SAEG +GLCISHGGGRRCQ  GC+KGAQGSTMFCKAHGGGKRC+ 
Sbjct: 352  RHGGGKRCQRENCTKSAEGLSGLCISHGGGRRCQSNGCTKGAQGSTMFCKAHGGGKRCTH 411

Query: 620  AGCSKGAEGSTPLCKAHGGGKRCLYNGGGICGKSVHGGTNFCVAHGGGKRCAVSGCTKSA 799
            +GC+KGAEGSTP CK HGGGKRC + G   C KSVHGGTNFCVAHGGGKRCAV  CTKSA
Sbjct: 412  SGCTKGAEGSTPFCKGHGGGKRCAFQGDDPCSKSVHGGTNFCVAHGGGKRCAVPECTKSA 471

Query: 800  RGRTDCCVRHGGGKRCKSEGCAKSAQGSTDFCKAHGGGKRCSWGDGKCE 946
            RGRTD CVRHGGGKRC+SEGC KSAQGSTDFCKAHGGGKRC+WG  + E
Sbjct: 472  RGRTDFCVRHGGGKRCQSEGCGKSAQGSTDFCKAHGGGKRCAWGQPETE 520

 Score =  114 bits (285), Expect = 2e-24
 Identities = 55/113 (48%), Positives = 65/113 (56%), Gaps = 15/113 (13%)
 Frame = +2

Query: 152 KRCRYFG---CKKGARGASGLGIGHGGGQRCQKPGCNKGAESRTAYCKAHGGGRRCNHLG 322
           KRC + G   C K   G +   + HGGG+RC  P C K A  RT +C  HGGG+RC   G
Sbjct: 432 KRCAFQGDDPCSKSVHGGTNFCVAHGGGKRCAVPECTKSARGRTDFCVRHGGGKRCQSEG 491

Query: 323 CTKSAEGKTDYCIAHGGGKRC--GYPDG----------CTKAARGKSGLCIRH 445
           C KSA+G TD+C AHGGGKRC  G P+           CT  ARGK+GLC  H
Sbjct: 492 CGKSAQGSTDFCKAHGGGKRCAWGQPETEYAGQSSSGPCTSFARGKTGLCALH 544

>ref|NP_176596.1| hypothetical protein; protein id: At1g64140.1 [Arabidopsis thaliana]
          Length = 658

 Score =  469 bits (1208), Expect = e-131
 Identities = 212/289 (73%), Positives = 238/289 (81%), Gaps = 5/289 (1%)
 Frame = +2

Query: 95   LQSGLQDTGITSE-----NRISNPKRCRYFGCKKGARGASGLGIGHGGGQRCQKPGCNKG 259
            + SG   +G++ +        S+ K C+  GC KGARGASG  I HGGG+RCQK GC+KG
Sbjct: 245  ISSGTCTSGLSQQLKPQLKNSSSSKLCQVEGCHKGARGASGRCISHGGGRRCQKHGCHKG 304

Query: 260  AESRTAYCKAHGGGRRCNHLGCTKSAEGKTDYCIAHGGGKRCGYPDGCTKAARGKSGLCI 439
            AE RT YCKAHGGGRRC  LGCTKSAEG+TD+CIAHGGG+RC + D CT+AARG+SGLCI
Sbjct: 305  AEGRTVYCKAHGGGRRCEFLGCTKSAEGRTDFCIAHGGGRRCSHED-CTRAARGRSGLCI 363

Query: 440  RHGGGKRCRIEGCARSAEGQAGLCISHGGGRRCQYLGCSKGAQGSTMFCKAHGGGKRCSF 619
            RHGGGKRC+ E C +SAEG +GLCISHGGGRRCQ  GC+KGAQGSTMFCKAHGGGKRC+ 
Sbjct: 364  RHGGGKRCQRENCTKSAEGLSGLCISHGGGRRCQSNGCTKGAQGSTMFCKAHGGGKRCTH 423

Query: 620  AGCSKGAEGSTPLCKAHGGGKRCLYNGGGICGKSVHGGTNFCVAHGGGKRCAVSGCTKSA 799
            +GC+KGAEGSTP CK HGGGKRC + G   C KSVHGGTNFCVAHGGGKRCAV  CTKSA
Sbjct: 424  SGCTKGAEGSTPFCKGHGGGKRCAFQGDDPCSKSVHGGTNFCVAHGGGKRCAVPECTKSA 483

Query: 800  RGRTDCCVRHGGGKRCKSEGCAKSAQGSTDFCKAHGGGKRCSWGDGKCE 946
            RGRTD CVRHGGGKRC+SEGC KSAQGSTDFCKAHGGGKRC+WG  + E
Sbjct: 484  RGRTDFCVRHGGGKRCQSEGCGKSAQGSTDFCKAHGGGKRCAWGQPETE 532

 Score =  114 bits (285), Expect = 2e-24
 Identities = 55/113 (48%), Positives = 65/113 (56%), Gaps = 15/113 (13%)
 Frame = +2

Query: 152 KRCRYFG---CKKGARGASGLGIGHGGGQRCQKPGCNKGAESRTAYCKAHGGGRRCNHLG 322
           KRC + G   C K   G +   + HGGG+RC  P C K A  RT +C  HGGG+RC   G
Sbjct: 444 KRCAFQGDDPCSKSVHGGTNFCVAHGGGKRCAVPECTKSARGRTDFCVRHGGGKRCQSEG 503

Query: 323 CTKSAEGKTDYCIAHGGGKRC--GYPDG----------CTKAARGKSGLCIRH 445
           C KSA+G TD+C AHGGGKRC  G P+           CT  ARGK+GLC  H
Sbjct: 504 CGKSAQGSTDFCKAHGGGKRCAWGQPETEYAGQSSSGPCTSFARGKTGLCALH 556

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 990,049,167
Number of Sequences: 1393205
Number of extensions: 28721171
Number of successful extensions: 133930
Number of sequences better than 10.0: 1646
Number of HSP's better than 10.0 without gapping: 90456
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 119474
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 53801056208
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf022g02 BP068988 1 449 2 MWL067f01_f AV769776 330 953

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