>KMC003125A_C01 KMC003125A_c01
TTAAAAATAAGAAACATCCGTTTTATCATTAGATATCAAAATTTTCTATCTCAAAACAAG
TCCAACCCCCGTGCTACAAACTACAGTAACTTCATGCAAAGGTTTACATCTATTTATGGT
ATAGATGAACAGCACTGAAAATGAGGAAACCTGCCATCTGCATCATAATCAAAAGGTTAA
ATTACCAAAAAGCCATGAAGAATTTTCTAAATGTGGCTCTCAAACAATCTGAAAATAGAG
AACTGGACCTGCCTTTAGGAAAGATTAATCACCTTTTGGGGATGCACATAAAGGTTTATC
CAGAGTATCAAAGTCGAAAATCAAAGATTATATTTCAACTTACCCAAGGTGTGTGTGTGT
GTGTGTGTGAGAGAGAGAGAGAGAGATCCTTACCATGGAAAATGATGCTCCAGTAATATG
GAGTAAGCTGTTGGTATAAATCTCTCATATTCATTGTGTCTGAAGGGGCGAACAGGAATC
TGCTTTGATAATGATAAGCTTGTGTATCCAAGTCTTTCATACTCAACTTTGAATTGGAAA
ACCCCATAGACATCTGGAACCTTAAATGATGTGAAATAACGACCCTTCCCGTCAGTTGAT
AAAGTCTTCAAGACATAGGGGCTCATCATGTAAAATTGCACTTGAACATCATTGGCTACA
TAGGGTTCCCAAGTTGTCCCAGACCACTCATAAATTTCAACAGAGTATTCCAGATCATCG
TTGATCCTATAGATGGATGGTTCATCAGCTTCACCGGCTTTATGGTGTTGCACATTCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003125A_C01 KMC003125A_c01
         (778 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_569038.1| dolichyl-di-phosphooligosaccharide-protein glyc...   219  3e-56
gb|AAM63023.1| dolichyl-di-phosphooligosaccharide-protein glycot...   219  3e-56
dbj|BAA97274.1| dolichyl-di-phosphooligosaccharide-protein glyco...   219  3e-56
gb|AAK59450.1| putative dolichyl-di-phosphooligosaccharide-prote...   216  2e-55
dbj|BAA23670.1| oligosaccharyltransferase [Homo sapiens]              127  2e-28

>ref|NP_569038.1| dolichyl-di-phosphooligosaccharide-protein glycotransferase
           (oligosaccharyltransferase)-like; protein id:
           At5g66680.1, supported by cDNA: 18419., supported by
           cDNA: gi_14334505, supported by cDNA: gi_16226811
           [Arabidopsis thaliana]
           gi|16226812|gb|AAL16268.1|AF428338_1 AT5g66680/MSN2_7
           [Arabidopsis thaliana] gi|23297258|gb|AAN12926.1|
           putative dolichyl-di-phosphooligosaccharide
           glycotransferase (oligosaccharyltransferase)
           [Arabidopsis thaliana]
          Length = 437

 Score =  219 bits (558), Expect = 3e-56
 Identities = 101/118 (85%), Positives = 110/118 (92%)
 Frame = -3

Query: 776 NVQHHKAGEADEPSIYRINDDLEYSVEIYEWSGTTWEPYVANDVQVQFYMMSPYVLKTLS 597
           N+ HH+ GE DEP+IYRI DDLE+SVEIYEWSG +WEPYVANDVQVQFYMMSPYVLKTLS
Sbjct: 296 NLVHHRVGETDEPAIYRIKDDLEFSVEIYEWSGKSWEPYVANDVQVQFYMMSPYVLKTLS 355

Query: 596 TDGKGRYFTSFKVPDVYGVFQFKVEYERLGYTSLSLSKQIPVRPFRHNEYERFIPTAY 423
           TD KG + TSFKVPDVYGVFQFKVEYE+LGYT+LSLSKQIPVRP+RHNEYERFIPTAY
Sbjct: 356 TDKKGLFHTSFKVPDVYGVFQFKVEYEKLGYTTLSLSKQIPVRPYRHNEYERFIPTAY 413

>gb|AAM63023.1| dolichyl-di-phosphooligosaccharide-protein glycotransferase
           (oligosaccharyltransferase)-like [Arabidopsis thaliana]
          Length = 437

 Score =  219 bits (558), Expect = 3e-56
 Identities = 101/118 (85%), Positives = 110/118 (92%)
 Frame = -3

Query: 776 NVQHHKAGEADEPSIYRINDDLEYSVEIYEWSGTTWEPYVANDVQVQFYMMSPYVLKTLS 597
           N+ HH+ GE DEP+IYRI DDLE+SVEIYEWSG +WEPYVANDVQVQFYMMSPYVLKTLS
Sbjct: 296 NLVHHRVGETDEPAIYRIKDDLEFSVEIYEWSGKSWEPYVANDVQVQFYMMSPYVLKTLS 355

Query: 596 TDGKGRYFTSFKVPDVYGVFQFKVEYERLGYTSLSLSKQIPVRPFRHNEYERFIPTAY 423
           TD KG + TSFKVPDVYGVFQFKVEYE+LGYT+LSLSKQIPVRP+RHNEYERFIPTAY
Sbjct: 356 TDKKGLFHTSFKVPDVYGVFQFKVEYEKLGYTTLSLSKQIPVRPYRHNEYERFIPTAY 413

>dbj|BAA97274.1| dolichyl-di-phosphooligosaccharide-protein glycotransferase
           (oligosaccharyltransferase)-like [Arabidopsis thaliana]
          Length = 442

 Score =  219 bits (558), Expect = 3e-56
 Identities = 101/118 (85%), Positives = 110/118 (92%)
 Frame = -3

Query: 776 NVQHHKAGEADEPSIYRINDDLEYSVEIYEWSGTTWEPYVANDVQVQFYMMSPYVLKTLS 597
           N+ HH+ GE DEP+IYRI DDLE+SVEIYEWSG +WEPYVANDVQVQFYMMSPYVLKTLS
Sbjct: 301 NLVHHRVGETDEPAIYRIKDDLEFSVEIYEWSGKSWEPYVANDVQVQFYMMSPYVLKTLS 360

Query: 596 TDGKGRYFTSFKVPDVYGVFQFKVEYERLGYTSLSLSKQIPVRPFRHNEYERFIPTAY 423
           TD KG + TSFKVPDVYGVFQFKVEYE+LGYT+LSLSKQIPVRP+RHNEYERFIPTAY
Sbjct: 361 TDKKGLFHTSFKVPDVYGVFQFKVEYEKLGYTTLSLSKQIPVRPYRHNEYERFIPTAY 418

>gb|AAK59450.1| putative dolichyl-di-phosphooligosaccharide-protein
           glycotransferase [Arabidopsis thaliana]
          Length = 437

 Score =  216 bits (551), Expect = 2e-55
 Identities = 100/118 (84%), Positives = 109/118 (91%)
 Frame = -3

Query: 776 NVQHHKAGEADEPSIYRINDDLEYSVEIYEWSGTTWEPYVANDVQVQFYMMSPYVLKTLS 597
           N+ HH+ GE DEP+IYRI DDLE+SVEIYEWSG +WEPYVAN VQVQFYMMSPYVLKTLS
Sbjct: 296 NLVHHRVGETDEPAIYRIKDDLEFSVEIYEWSGKSWEPYVANGVQVQFYMMSPYVLKTLS 355

Query: 596 TDGKGRYFTSFKVPDVYGVFQFKVEYERLGYTSLSLSKQIPVRPFRHNEYERFIPTAY 423
           TD KG + TSFKVPDVYGVFQFKVEYE+LGYT+LSLSKQIPVRP+RHNEYERFIPTAY
Sbjct: 356 TDKKGLFHTSFKVPDVYGVFQFKVEYEKLGYTTLSLSKQIPVRPYRHNEYERFIPTAY 413

>dbj|BAA23670.1| oligosaccharyltransferase [Homo sapiens]
          Length = 456

 Score =  127 bits (319), Expect = 2e-28
 Identities = 58/117 (49%), Positives = 78/117 (66%)
 Frame = -3

Query: 773 VQHHKAGEADEPSIYRINDDLEYSVEIYEWSGTTWEPYVANDVQVQFYMMSPYVLKTLST 594
           V HH+ GE   P+ Y + D +EYS+ I + S   W P+  +D+Q++F  + P+V   L  
Sbjct: 311 VSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNAKWVPFDGDDIQLEFVRIDPFVRTFLKK 370

Query: 593 DGKGRYFTSFKVPDVYGVFQFKVEYERLGYTSLSLSKQIPVRPFRHNEYERFIPTAY 423
            G G+Y   FK+PDVYGVFQFKV+Y RLGYT L  S Q+ VRP +H +YERFIP+AY
Sbjct: 371 KG-GKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAY 426

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 674,917,927
Number of Sequences: 1393205
Number of extensions: 14931212
Number of successful extensions: 35051
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 31893
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34111
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 38375267554
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD081a06_f BP050432 1 537 2 GNf051f12 BP071173 70 608 3 MWL034f06_f AV769155 137 434 4 MR035d04_f BP078699 140 440 5 GNf085f02 BP073650 156 637 6 MPD010a09_f AV770633 182 405 7 MPD063f06_f AV774215 199 636 8 SPD073d10_f BP049833 205 491 9 MPD056b12_f AV773738 206 637 10 GNf089f10 BP073956 210 613 11 SPDL031b01_f BP053918 211 754 12 MF021c08_f BP029367 220 586 13 MFB032h04_f BP036381 224 684 14 MFL017c06_f BP033821 227 776 15 MR051c02_f BP079931 238 639 16 SPD087e02_f BP050950 239 785 17 MPD099f10_f AV776477 241 312 18 MF019f08_f BP029270 245 636 19 GNf021f09 BP068911 249 740

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