Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003100A_C03 KMC003100A_c03
(634 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9M571|PEAM_SPIOL Phosphoethanolamine N-methyltransferase gi|... 154 7e-39
ref|NP_175293.1| phosphoethanolamine N-methyltransferase, putati... 150 4e-38
dbj|BAC57432.1| phosphoethanolamine N-methyltransferase [Suaeda ... 144 1e-35
ref|NP_177501.1| phosphoethanolamine N-methyltransferase, putati... 142 3e-35
sp|Q9C6B9|PEM3_ARATH Putative phosphoethanolamine N-methyltransf... 142 3e-35
>sp|Q9M571|PEAM_SPIOL Phosphoethanolamine N-methyltransferase
gi|7407189|gb|AAF61950.1|AF237633_1 phosphoethanolamine
N-methyltransferase [Spinacia oleracea]
Length = 494
Score = 154 bits (390), Expect(2) = 7e-39
Identities = 69/104 (66%), Positives = 91/104 (87%)
Frame = -3
Query: 632 RSFYKWLKPGGKLLITDYCKSAGSPSLEFAEYIKKGGYYIHDMKAYEQMLENAGFDDIIA 453
RSF+KWLKPGGK+LI+DYCKSAG+PS EFA YI++ GY +HD+KAY +ML++AGF ++IA
Sbjct: 372 RSFHKWLKPGGKVLISDYCKSAGTPSAEFAAYIRQRGYDLHDVKAYGKMLKDAGFVEVIA 431
Query: 452 ENRTDLFVKTLKQELEALENKKDDFICEFGEEDYNEIVERWKRK 321
ENRTD F++ L++EL+ALE +KDDFI +F EEDYN+IV+ WK K
Sbjct: 432 ENRTDQFIQVLQKELDALEQEKDDFIDDFSEEDYNDIVDGWKAK 475
Score = 28.1 bits (61), Expect(2) = 7e-39
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = -1
Query: 340 WKDGSAKQIRGESLEQMWGLFIAEK 266
WK AK +R EQ WGLFIA+K
Sbjct: 472 WK---AKLVRTTEGEQQWGLFIAKK 493
>ref|NP_175293.1| phosphoethanolamine N-methyltransferase, putative; protein id:
At1g48600.1, supported by cDNA: gi_16226648, supported
by cDNA: gi_17380783 [Arabidopsis thaliana]
gi|24212079|sp|Q944H0|PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2
gi|16226649|gb|AAL16223.1|AF428454_1 At1g48600/T1N15_20
[Arabidopsis thaliana] gi|17380784|gb|AAL36222.1|
putative phosphoethanolamine N-methyltransferase
[Arabidopsis thaliana] gi|22136802|gb|AAM91745.1|
putative phosphoethanolamine N-methyltransferase
[Arabidopsis thaliana]
Length = 475
Score = 150 bits (378), Expect(2) = 4e-38
Identities = 67/104 (64%), Positives = 89/104 (85%)
Frame = -3
Query: 632 RSFYKWLKPGGKLLITDYCKSAGSPSLEFAEYIKKGGYYIHDMKAYEQMLENAGFDDIIA 453
R+F+KWLKPGGK+LITDYC+SA +PS EFAEYIK+ GY +HD++AY QML++AGFDD+IA
Sbjct: 353 RTFFKWLKPGGKVLITDYCRSAETPSPEFAEYIKQRGYDLHDVQAYGQMLKDAGFDDVIA 412
Query: 452 ENRTDLFVKTLKQELEALENKKDDFICEFGEEDYNEIVERWKRK 321
E+RTD FV+ L++ELE +E +K++FI +F EEDYN+IV W K
Sbjct: 413 EDRTDQFVQVLRRELEKVEKEKEEFISDFSEEDYNDIVGGWSAK 456
Score = 30.0 bits (66), Expect(2) = 4e-38
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = -1
Query: 328 SAKQIRGESLEQMWGLFIAEKK 263
SAK R S EQ WGLFIA+KK
Sbjct: 454 SAKLERTASGEQKWGLFIADKK 475
>dbj|BAC57432.1| phosphoethanolamine N-methyltransferase [Suaeda japonica]
Length = 494
Score = 144 bits (363), Expect(2) = 1e-35
Identities = 65/104 (62%), Positives = 84/104 (80%)
Frame = -3
Query: 632 RSFYKWLKPGGKLLITDYCKSAGSPSLEFAEYIKKGGYYIHDMKAYEQMLENAGFDDIIA 453
RSFYKWLKPGGK+LI+DYCK AG PS EFA YIK+ GY +HD+K Y QML++AGF D++A
Sbjct: 372 RSFYKWLKPGGKVLISDYCKKAGPPSPEFAAYIKQRGYDLHDVKEYGQMLKDAGFVDVLA 431
Query: 452 ENRTDLFVKTLKQELEALENKKDDFICEFGEEDYNEIVERWKRK 321
E+RT+ F++ L++ELE +E +KD FI +F EEDYN+IV W K
Sbjct: 432 EDRTEQFIRVLRKELETVEKEKDVFISDFSEEDYNDIVGGWNDK 475
Score = 27.7 bits (60), Expect(2) = 1e-35
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -1
Query: 340 WKDGSAKQIRGESLEQMWGLFIAEKK 263
W D + +GE Q WGLF+A+KK
Sbjct: 472 WNDKLRRTAKGE---QRWGLFVAKKK 494
>ref|NP_177501.1| phosphoethanolamine N-methyltransferase, putative; protein id:
At1g73600.1 [Arabidopsis thaliana]
gi|25406280|pir||H96762 hypothetical protein F6D5.1
[imported] - Arabidopsis thaliana
gi|12323692|gb|AAG51806.1|AC079676_1 phosphoethanolamine
N-methyltransferase, putative; 6854-3993 [Arabidopsis
thaliana]
Length = 555
Score = 142 bits (359), Expect(2) = 3e-35
Identities = 63/104 (60%), Positives = 86/104 (82%)
Frame = -3
Query: 632 RSFYKWLKPGGKLLITDYCKSAGSPSLEFAEYIKKGGYYIHDMKAYEQMLENAGFDDIIA 453
R FYKWLKPGGK+LITDYC+S +PS +FA YIKK GY +HD++AY QML +AGF+++IA
Sbjct: 433 RRFYKWLKPGGKVLITDYCRSPKTPSPDFAIYIKKRGYDLHDVQAYGQMLRDAGFEEVIA 492
Query: 452 ENRTDLFVKTLKQELEALENKKDDFICEFGEEDYNEIVERWKRK 321
E+RTD F+K LK+EL+A+E +K++FI +F +EDY +I+ WK K
Sbjct: 493 EDRTDQFMKVLKRELDAVEKEKEEFISDFSKEDYEDIIGGWKSK 536
Score = 27.7 bits (60), Expect(2) = 3e-35
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -1
Query: 340 WKDGSAKQIRGESLEQMWGLFIAEK 266
WK +K +R S EQ WGLFIA++
Sbjct: 533 WK---SKLLRSSSGEQKWGLFIAKR 554
>sp|Q9C6B9|PEM3_ARATH Putative phosphoethanolamine N-methyltransferase 3
Length = 490
Score = 142 bits (359), Expect(2) = 3e-35
Identities = 63/104 (60%), Positives = 86/104 (82%)
Frame = -3
Query: 632 RSFYKWLKPGGKLLITDYCKSAGSPSLEFAEYIKKGGYYIHDMKAYEQMLENAGFDDIIA 453
R FYKWLKPGGK+LITDYC+S +PS +FA YIKK GY +HD++AY QML +AGF+++IA
Sbjct: 368 RRFYKWLKPGGKVLITDYCRSPKTPSPDFAIYIKKRGYDLHDVQAYGQMLRDAGFEEVIA 427
Query: 452 ENRTDLFVKTLKQELEALENKKDDFICEFGEEDYNEIVERWKRK 321
E+RTD F+K LK+EL+A+E +K++FI +F +EDY +I+ WK K
Sbjct: 428 EDRTDQFMKVLKRELDAVEKEKEEFISDFSKEDYEDIIGGWKSK 471
Score = 27.7 bits (60), Expect(2) = 3e-35
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -1
Query: 340 WKDGSAKQIRGESLEQMWGLFIAEK 266
WK +K +R S EQ WGLFIA++
Sbjct: 468 WK---SKLLRSSSGEQKWGLFIAKR 489
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 494,148,801
Number of Sequences: 1393205
Number of extensions: 9850746
Number of successful extensions: 25342
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 24651
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25323
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26154777244
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
