Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003072A_C03 KMC003072A_c03
(594 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM13199.1| alpha-galactosidase-like protein [Arabidopsis tha... 244 6e-64
ref|NP_196455.1| glycosyl hydrolase family 27 (alpha-galactosida... 244 6e-64
pir||T04422 alpha-galactosidase (EC 3.2.1.22) - barley (fragment... 201 8e-51
gb|AAG13536.1|AC023240_9 putative alpha-galactosidase [Oryza sat... 190 1e-47
dbj|BAC55816.1| putative alpha-galactosidase [Oryza sativa (japo... 181 6e-45
>gb|AAM13199.1| alpha-galactosidase-like protein [Arabidopsis thaliana]
Length = 410
Score = 244 bits (623), Expect = 6e-64
Identities = 118/143 (82%), Positives = 131/143 (91%)
Frame = -1
Query: 594 GMTKDEYIVHFSLWAISKAPLLLGCDVRNVTKETMEIVSNKEVIAVNQDPLGVQAKKVRM 415
GMTKDEYIVHFS+WAISKAPLLLGCD+RN+TKETMEIV+NKEVIA+NQDP GVQAKKVRM
Sbjct: 269 GMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRM 328
Query: 414 EGDLEIWAGPLSGYKVALVLLNRGPQQRQSITANWDDIGIPPKSAVEARDLWEHKTLKRP 235
EGDLE+WAGPLSGY+VAL+LLNRGP R SITA W+DI IP S VEARDLWEH+TLK+
Sbjct: 329 EGDLEVWAGPLSGYRVALLLLNRGP-SRTSITALWEDIEIPANSIVEARDLWEHQTLKQK 387
Query: 234 FVDKLTATVDSHACKMYVLKPIA 166
FV LTATVDSHACK+YVLKP+A
Sbjct: 388 FVGNLTATVDSHACKLYVLKPVA 410
>ref|NP_196455.1| glycosyl hydrolase family 27 (alpha-galactosidase/melibiase);
protein id: At5g08380.1, supported by cDNA: gi_20260601
[Arabidopsis thaliana] gi|10178280|emb|CAC08338.1|
alpha-galactosidase-like protein [Arabidopsis thaliana]
Length = 410
Score = 244 bits (623), Expect = 6e-64
Identities = 118/143 (82%), Positives = 131/143 (91%)
Frame = -1
Query: 594 GMTKDEYIVHFSLWAISKAPLLLGCDVRNVTKETMEIVSNKEVIAVNQDPLGVQAKKVRM 415
GMTKDEYIVHFS+WAISKAPLLLGCD+RN+TKETMEIV+NKEVIA+NQDP GVQAKKVRM
Sbjct: 269 GMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRM 328
Query: 414 EGDLEIWAGPLSGYKVALVLLNRGPQQRQSITANWDDIGIPPKSAVEARDLWEHKTLKRP 235
EGDLE+WAGPLSGY+VAL+LLNRGP R SITA W+DI IP S VEARDLWEH+TLK+
Sbjct: 329 EGDLEVWAGPLSGYRVALLLLNRGP-SRTSITALWEDIEIPANSIVEARDLWEHQTLKQK 387
Query: 234 FVDKLTATVDSHACKMYVLKPIA 166
FV LTATVDSHACK+YVLKP+A
Sbjct: 388 FVGNLTATVDSHACKLYVLKPVA 410
>pir||T04422 alpha-galactosidase (EC 3.2.1.22) - barley (fragment)
gi|2204224|emb|CAA74161.1| alpha-galactosidase [Hordeum
vulgare subsp. vulgare]
Length = 159
Score = 201 bits (510), Expect = 8e-51
Identities = 92/142 (64%), Positives = 117/142 (81%)
Frame = -1
Query: 594 GMTKDEYIVHFSLWAISKAPLLLGCDVRNVTKETMEIVSNKEVIAVNQDPLGVQAKKVRM 415
GMT DEYIVHFS+WAISKAPL++GCDVR+++++T +I++NKEVIAVNQDPLGVQ KKVRM
Sbjct: 13 GMTNDEYIVHFSIWAISKAPLIIGCDVRHMSQDTYDILANKEVIAVNQDPLGVQGKKVRM 72
Query: 414 EGDLEIWAGPLSGYKVALVLLNRGPQQRQSITANWDDIGIPPKSAVEARDLWEHKTLKRP 235
EG EIWA PL+GY+ A++LLNR + ITA+WDDIG+P +AVEARDLW HKTL+
Sbjct: 73 EGSSEIWAAPLTGYRTAVLLLNRHAKDEAQITAHWDDIGLPAGTAVEARDLWLHKTLEPK 132
Query: 234 FVDKLTATVDSHACKMYVLKPI 169
F DK++ V HA +M+VL P+
Sbjct: 133 FTDKMSFNVTPHAARMFVLTPL 154
>gb|AAG13536.1|AC023240_9 putative alpha-galactosidase [Oryza sativa]
Length = 413
Score = 190 bits (483), Expect = 1e-47
Identities = 88/145 (60%), Positives = 115/145 (78%), Gaps = 3/145 (2%)
Frame = -1
Query: 594 GMTKDEYIVHFSLWAISKAPLLLGCDVRNVTKETMEIVSNKEVIAVNQDPLGVQAKKVRM 415
GMT DEYIVHFSLWAISKAPL++GCDVR++++ET +I++NKEVIAVNQD LG+Q KKVRM
Sbjct: 263 GMTNDEYIVHFSLWAISKAPLIIGCDVRHMSQETYDILANKEVIAVNQDALGIQGKKVRM 322
Query: 414 EGDLEIWAGPLSGYKVALVLLNRGPQQRQSITANWDDIGIPPKSAVEARDLWEHKTLK-- 241
EG E+WA PLSGY+ A++LLNR + +ITA+WDD+G+P +AVEARDLW H T+
Sbjct: 323 EGSSEVWAAPLSGYRTAVLLLNRHAAEAAAITAHWDDVGLPAGTAVEARDLWRHATVAGA 382
Query: 240 -RPFVDKLTATVDSHACKMYVLKPI 169
+K+T V H+C+M +LKP+
Sbjct: 383 GGELTEKITLDVAPHSCRMLLLKPL 407
>dbj|BAC55816.1| putative alpha-galactosidase [Oryza sativa (japonica
cultivar-group)]
Length = 390
Score = 181 bits (459), Expect = 6e-45
Identities = 84/143 (58%), Positives = 111/143 (76%), Gaps = 1/143 (0%)
Frame = -1
Query: 594 GMTKDEYIVHFSLWAISKAPLLLGCDVRNVTKETMEIVSNKEVIAVNQDPLGVQAKKVR- 418
GMT DEY+VH SLWAI+KAPL++GCDVR++++ETMEI+SN EVIA+NQDPLGVQ KKVR
Sbjct: 240 GMTNDEYVVHISLWAIAKAPLIIGCDVRSISRETMEILSNPEVIAINQDPLGVQGKKVRK 299
Query: 417 MEGDLEIWAGPLSGYKVALVLLNRGPQQRQSITANWDDIGIPPKSAVEARDLWEHKTLKR 238
+ ++E+WAGPLS + A++LLNRG + ITA W DIG+ P AVEA+++W H T
Sbjct: 300 YDNEIEVWAGPLSQQRTAVLLLNRGATGSRQITAAWQDIGVGPGVAVEAKNVWLHATAPG 359
Query: 237 PFVDKLTATVDSHACKMYVLKPI 169
F LTA V +H+CK++VL P+
Sbjct: 360 RFTGSLTAEVAAHSCKLFVLTPV 382
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 507,116,929
Number of Sequences: 1393205
Number of extensions: 10533837
Number of successful extensions: 30188
Number of sequences better than 10.0: 91
Number of HSP's better than 10.0 without gapping: 29267
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30121
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22854740960
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
